• BMB Reports
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• v.35 no.4
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• pp.403-408
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• 2002

A hypothetical 21.0 kDa protein (ORF O197) from Escherichia coli K-12 was cloned, purified, and characterized. The protein sequence of ORF O197(termed EcO197) shares a 33.5% identity with that of a novel NTPase from Methanococcus jannaschii. The EcO197 protein was purified using Ni-NTA affinity chromatography, protease digestion, and gel filtration column. It hydrolyzed nucleoside triphosphates with an O6 atom-containing purine base to nucleoside monophosphate and pyrophosphate. The EcO197 protein had a strong preference for deoxyinosine triphosphate (dITP) and xanthosine triphosphate (XTP), while it had little activity in the standard nucleoside triphosphates (dATP, dCTP, dGTP, and dTTP). These aberrant nucleotides can be produced by oxidative deamination from purine nucleotides in cells; they are potentially mutagenic. The mutation protection mechanisms are caused by the incorporation into DNA of unwelcome nucleotides that are formed spontaneously. The EcO197 protein may function to eliminate specifically damaged purine nucleotide that contains the 6-keto group. This protein appears to be the first eubacterial dITP-and XTP-hydrolyzing enzyme that has been identified.

• Magazine of the Korean Society of Agricultural Engineers
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• v.31 no.1
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• pp.96-105
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• 1989

This laboratory study was performed to produce basic data for the estimation of water requirements for desalinization, through analyzing changes of the electrical conductivity and the exchangeable sodium percentage during the desalinization of reclaimed tidelands. Desalinization experiments were carried out by two water management practices, namely, the leaching method by subsurface drainage and the rinsing method by surface drainage, using samples of silt loam soil and silty clay loam soil collected in reclaimed tidelands. The results obtained from this study were summarized as follows : 1. The sample soils used in this study were saline-sodic soils with the high electrical conductivity and the high exchangeable sodium percentage. 2. Changes of the electrical conductivity and the exchangeable sodium percentage with water requirements for desalinization showed a similar tendency in the desalinization experiment by the same water management practice. 3. The regression equation between the relative electrical conductivity(EC/EC) and water requirements for desalinization(Dw/Ds) could be described by Dw/Ds=O. 29x(EC/EC.) -0.982 (Leaching method), Dw/Ds=3. 678X0. 030(EC/EC ) (Rinsing method). 4. The regression equation between the relative exchangeable sodium percentage (ESP/ESP ) and water requirements for desalinization (Dw/Ds) could be expressed in Dw/Ds = 0.039 x (ESP/ESP. ) - 1. 134 (Leaching method), Dw/Ds=7. 197X0. 024(ESP/ESP ) (Rinsing method). 5. It was estimated that water requirements for the adequate desalinization would be Dw/Ds=0.3 (Leaching method) and Dw/Ds=3.0 (Rinsing method)

• Journal of Industrial Technology
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• v.26 no.A
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• pp.189-197
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• 2006

Water shortage as well as water control problems including urban flood and drought have led to developing technologies for rainwater utilization in order to provide a sustainable water supply. However, relatively few works have been done to improve the water quality during rainwater utilization. Therefore, in this study, the characteristics of rainwater were examined in terms of pollutant concentrations and outflow rate. A experimental rain and roof model($1m{\times}1m$) were produced to evaluate wash-off properties of pollutants. And rainfall intensity were standardized in 10 mm/hr, a experimental model roof catchment surface were coated in $TiO_2$ photocatalyst. Samples of runoff rainwater collected from a experimental model were analyzed for physicochemical parameters such as Turbidity, Suspended Solid, EC. The results show that the first flush of rainwater contains substantial amount of contaminants that potentially pollute the whole rainwater. Surface treatment of roof catchment area using $TiO_2$ photocatalyst allowed a better runoff property of rainwater because of its improved hydrophilicity.

• Journal of the Korean Electrochemical Society
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• v.15 no.3
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• pp.190-197
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• 2012

We employed the vinyl ethylene carbonate (VEC) as an electrolyte additive and investigated the effect of the electrolyte additive on the electrochemical performance in hybrid capacitor. The activated carbon was adopted as cathode material, and the $Li_4Ti_5O_{12}$ oxide was used as anode material. The electrolyte was prepared with the $LiPF_6$ salt in the mixed solvent of ethylene carbonate (EC), dimethyl carbonate (DMC), and ethyl methyl carbonate(EMC). We evaluated the electrochemical performance of the hybrid capacitor with increasing the amount of the VEC electrolyte additive, which is known as the remover of oxygen functional group and the stabilizer of the electrode by reducing the surface of electrode, and obtained the superior performance data especially at the addition of the VEC electrolyte additive of around 0.7 vol%. On the contrary, the addition of the VEC more than 0.7 vol% in the electrolyte leads to the degradation in electrochemical performance of hybrid capacitor, suggesting the increase of the side reaction from the excessive VEC additive. X-ray photoelectron spectroscopy (XPS) revealed that the addition of the VEC suppressed the formation of LiF component, which is known as the insulator, on the surface of electrode. The optimized addition of VEC exhibited the improved capacity retention around 82.7% whereas the bare capacitors without VEC additive showed the 43.2% of capacity retention after 2500 cycling test.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.6
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• pp.983-991
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• 2012

These days, agricultural products cultivated in facilities occupy the highest percentage of agricultural output price. Specifically cucumbers have been one of the crops that farmers prefer to growing, because their prices were high. However, cucumber crop is sensitive to the soil and environments and it requires the exact crop management. In order to establish cultivation techniques for cucumbers, the current situation of cucumber cultivation was surveyed from ten cucumber farmlands; five farmlands of cucumber cultivation in forcing and five of semi-forcing practicing systems, respectably. The soil conditions were alluvial or valley in soil topology, moderately or poorly drainage in soil drainage classes, coarse loamy in soil texture family. Soil was managed with deep plowing combined with application of basal fertilizers such as compost, rice straw, oil cake, wood chip and chemical fertilizer. The whole soil was prepared in uniformly with rotary. Three major nutrients ($N-P_2O_5-K_2O$)of basal application were 815-464-529 kg $ha^{-1}$ in forcing and 197-135-151 kg $ha^{-1}$ in semi-forcing cultivation. Top dressing of fertilizer was supplied in fertigation system of macro and micro elements in 2~3 day interval with water irrigation. The average yields of cucumbers were $168t\;ha^{-1}$ with 381,000 thousand won $ha^{-1}$ in average gross profit (AGP) in forcing cultivation and $115t\;ha^{-1}$ with 177,000 thousand won $ha^{-1}$ in AGP in semi-forcing cultivation. Cucumber production during the winter season was considered to increase the gross profit because cucumber price tends to stay in high level during this time. The accumulation of soil chemicals like EC, available $P_2O_5$ and exchangeable cations could be controlled by rice straw application. The rice straw application increased soil temperature during the winter season, in exchange of soil air, and in extension of plant roots. In addition, the rice straw application somewhat affected decrease of salts accumulation.

• Korean Journal of Agricultural Science
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• v.45 no.2
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• pp.197-203
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• 2018

Biochar is the carbon solid produced through the pyrolysis of a biomass from organic sources such as agricultural waste, animal manure, and sludge under limited or anaerobic conditions. Biochar has the effect of reducing greenhouse gases through the carbon sequestration method; additionally, biochar is known to function as a soil amendment. This experiment was conducted to evaluate the application of biochar on the growth characteristics of Chinese cabbage at Chungnam National University in Daejeon, Korea. The Chinese cabbage was grown for 50 days in a glasshouse in pots. A pruning branch was used to produce the bead and pellet forms of biochar through pyrolysis. The biochar was added to the soil at 0, 2, and 5% by weight. The Chinese cabbage with the 2% treatment of the bead form of biochar had the highest fresh weight ($149.43{\pm}15.92g\;plant^{-1}$) which was increased by 10% compared to the control ($136.91{\pm}31.46g\;plant^{-1}$). Moreover, for the 5% treatment of the bead form of biochar ($60.91{\pm}9.82g\;plant^{-1}$), the growth decreased by 57% compared to the control. As the content of the bead form of biochar increased, the shoot dry weight, leaf number, leaf length and lead width that appeared decreased. An increase in the total organic matter, Avail. $P_2O_5$, Ex. cation and EC was observed when the biochar content was increased. Our results support the application of 2% biochar in the bead form for increased growth of Chinese cabbage.

• Journal of Animal Science and Technology
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• v.49 no.1
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• pp.137-146
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• 2007

This study was conducted to determine fertilizer nutrient and pollutant production of Holstein dairy cattle by estimating manure characteristics. The moisture content of feces was 83.9% and 95.1% for urine. The pH of feces and urine were in the ranges of 7.0～7.4 and 7.5～7.8, respectively. The average BOD5, COD, SS, T-N, T-P concentrations of the dairy feces were 18,294, 52,765, 102,889, 2,575, and 457mg/ℓ, respectively. Dairy urine showed lower levels of BOD5(5,455mg/ℓ), COD(8,089mg/ℓ), SS(593mg/ℓ), T-N(3,401mg/l), and T-P(13mg/ℓ) than feces. The total daily produced pollutant amounts of a dairy cow were 924.1g(Milking cow), 538.8g(Dry cow), 284.4g(Heifer) of BOD5, 2,336.5g (Milking cow), 1,651.8g(Dry cow), 734.1g(Heifer) of COD and 4,210.1g(Milking cow), 2,417.1g(Dry cow), 1,629.1g(Heifer) of SS and 194.8g(Milking cow), 96.4g(Dry cow), 58.3g(Heifer) of T-N and 24.0g(Milking cow), 10.2g(Dry cow), 6.1g(Heifer) of T-P. The calculated amount of pollutants produced by a 450kg dairy cow for one year were 181.3kg of BOD5, 492.5kg of COD, 899.9kg of SS, 36.0kg of T-N and 4.1kg of T-P. The total yearly estimated pollutant production from all head(497,261) of dairy cattle in Korea is 90,149 tons of BOD5, 244,890 tons of COD, 447,491 tons of SS, 17,898 tons of T-N and 2,008 tons of T-P. The fertilizer nutrient concentrations of dairy feces was 0.26% N, 0.1% P2O5 and 0.14% K2O. Urine was found to contain 0.34% N, 0.003% of P2O5 and 0.31% K2O. The total daily fertilizer nutrients produced by dairy cattle were 197.4g (Milking cow), 97.4g(Dry cow), and 57.9g(Heifer) of Nitrogen, 54.2g(Milking cow), 22.2g(Dry cow), and 14.2g(Heifer) of P2O5 and 110.8g(Milking cow), 80.4g (Dry cow), and 39.5g(Heifer) of K2O. The total yearly estimated fertilizer nutrient produced by a 450kg dairy animal is 36.2kg of N, 8.8kg of P2O5, 24.6kg of K2O. The estimated yearly fertilizer nutrient production from all dairy cattle in Korea is 18,000 tons of N, 4,397 tons of P2O5, 12,206 tons of K2O. Dairy manure contains useful trace minerals for crops, such as CaO and MgO, which are contained in similar levels to commercial compost being sold in the domestic market. Concentrations of harmful trace minerals, such as As, Cd, Hg, Pb, Cr, Cu, Ni, Zn, met the Korea compost standard regulations, with some of these minerals being in undetected amounts.

• Journal of Environmental Impact Assessment
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• v.24 no.4
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• pp.352-366
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• 2015

The purpose of this study is to offer the basic data to the habitat conservation and management by surveying and analysing the ecological characteristics such as the flora, vegetation structure and soil of Rhodotypos scandens habitat. The flora were summarized as 131 taxa including 57 families, 99 genera, 107 species, 1 subspecies, 17 varieties and 6 forms. The life forms grouped as in the follows; the megaphanerophytes(MM) were 21 taxa, 24 taxa of the microphanerophytes(M), 30 taxa of nanophanerophytes(N), 6 taxa of chamaiphytes(Ch), 20 taxa of hemicryptophytes(H), 17 taxa of geophytes(G), 12 taxa of therophytes(Th) and 1 taxa of hydrophytes(HH). The present conditions of communities were 8 types including Zelkova serrata, Quercus aliena, Ulmus parvifolia, Rhamnella frangulioides, Castanea crenata, Albizia julibrissin, Celtis sinensis and Robinia pseudoacacia. In case of the dominant species by layers, the tree layer species were Zelkova serrata, Quercus aliena, Ulmus parvifolia, Castanea crenata, Albizia julibrissin, Celtis sinensis and Robinia pseudoacacia, and the subtree layer species were Rhamnella frangulioides, Q. aliena, Z. serrata, A. julibrissin, U. parvifolia and Broussonetia kazinoki. The shrub layer species was Rhodotypos scandens. In the results of analyzing the diversity index, $H^{\prime}{_{max}}$ from 1.691 to 2.610, from 2.197 to 3.466 in H'max, from 0.646 to 0.903 in J' and from 0.097 to 0.354 in D. In the results of analysing the soil, there showed that acidity was 5.6, 0.5dS/m of electrical conductivity(EC), 59.1mg/kg of available $P_2O_5$, 49.7% of organic matter content, $0.4cmol^+/kg$ of exchangeable $K^+$, $13.5cmol^+/kg$ of exchangeable $Ca^{2+}$, $3.3cmol^+/kg$ of exchangeable $Mg^{2+}$.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.10 no.3
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• pp.189-197
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• 2012

Geochemical composition of fracture filling minerals and groundwater was investigated to characterize geochemical characteristics of groundwater system at the KURT site. Minerals such as calcite, illite, laumontite, chlorite, epidote, montmorillonite, and kaolinite, as well as I/S mixed layer minerals were detected in the minerals extracted from the fracture surfaces of the core samples. The groundwater from the DB-1, YS-1 and YS-4 boreholes showed alkaline conditions with pH of higher than 8. The electrical conductivity (EC) values of the groundwater samples were around $200{\mu}S/cm$, except for the YS-1 borehole. Dissolved oxygen was almost zero in the DB-1 borehole indicating highly reduced conditions. The Cl- concentration was estimated around 5 mg/L and showed homogeneous distribution along depths at the KURT site. It might indicate the mixing between shallow groundwater and deep groundwater. The shallow groundwater from boreholes showed $Ca-HCO_3$ type, whereas deep groundwater below 300 m from the surface indicated $Na-HCO_3$ type. The isotopic values observed in the groundwater ranged from -10.4 to -8.2‰ for ${\delta}^{18}O$ and from -71.3 to -55.0‰for ${\delta}D$. In addition, the isotope-depleted water contained higher fluoride concentration. The oxygen and hydrogen isotopic values of deep groundwater were more depleted compared to the shallow groundwater. The results from age dating analysis using $^{14}C$ indicated relatively younger (2000~6000yr old) groundwater compared to other european granitic groundwaters such as Stripa (Sweden).