• Journal of Plant Biology
• /
• v.39 no.3
• /
• pp.197-202
• /
• 1996

A pollen-specific cDNA clone, LMP50, was isolated from the mature pollen cDNA library of the Easter lily. The LMP50 transcript was highly abundant in mture pollen grains but not detectable in other organs. The LMP50 cDNA clone contains 1383 nucleotides and two open reading frames. The first codes for a peptide of 15 amino acid residues. The role of this peptide is nuclear. The second encodes a protein containing 329 amino acid residues. This protein exhibited a significant homology to human tartrate-resistant acid phosphatase and porcine uteroferrin. Both of these enzymes have been suggested to play a role in iron transport. Therefore, LMP50 may act as an iron carrier protein in mature pollen grains.

• Korean Journal of Agricultural Science
• /
• v.1 no.1
• /
• pp.83-89
• /
• 1974

Georgia lily bulbs from experimental plot of the Chungnam National University, weighting 14.1~18.0gm, were used for this study. These bulbs were treated for the natural cooling at cold frame for 90 days and refrigerating at $8^{\circ}C{\pm}1^{\circ}C$ for 50~90 days. And then, these bulbs were planted in the vinyl house. The results of forcing for each treatments were as follow; 1) The natural cooling treatment gave 3 days ealier flowering than the refrigeratings. 2) In the blooming rate, the height of plant, and the size of flower, all refrigerating treatments were bigger than the natural cooling. 3) Among the refrigerating treatments, the best results were attain in the 50 and 60 days treatments than others. 4) Therefore, it is recommended that Georgia lily, as the next crop after Chrysanthemum ect, can be forced effectively by refrigerating treatment at $8^{\circ}C$ for 50~60 days, if these bulbs can be planted until the end of December.

• Journal of Plant Biology
• /
• v.39 no.1
• /
• pp.9-13
• /
• 1996

A clone, LCM1, which encodes calmodulin (CaM) was isolated and characterized from monocot lily (Lilium longiflorum Thunb.) plants. The clone is 681 bps and contains the 447 bp coding region, 8 bp leader sequence, 210 bp 3'-untraslated region, and a poly(A) tail. The coding region of 149 amino acids encodes a protein of predicted Mr 17 kD. Comparison of the LCM1 amino acid sequence with other CaMs revealed that the protein is highly conserved among various living organisms. The expression level of calmodulin gene in lily was studied by RNA blot analysis. The LCM1 mRNA was present in all tissues tested. However, a higher level of calmodulin was observed in anther and floral bud. The level of calmodulin mRNA in anther was about 10 times higher than that in anther was about 10 times higher than that in vegetative tissues. The anther preferential expression of CaM in lily is currently investigated in dicot plants.

• Journal of Plant Biotechnology
• /
• v.6 no.1
• /
• pp.9-13
• /
• 2004

Transient uidA expression was used to optimize parameters required for biolistic transformation of suspension cells of Easter lily, Lilium longiflourm. Maximum uidA expression occurred following bombardment with gold particles as compared to tungsten. A 3hr pre-treatment of suspension cells with 0.125M osmoticum resulted in a 1.5X increase in uidA expression. A helium pressure of 1550 psi combined with a particle travelling distance of 6cm resulted in maximum uidA expression as compared to either 1100, 1200, or 1800 psi. Transient transformation resulted in up to 493 uidA expressing cells/Petri plate. For stable transformation suspension cells of Lilium longiflorum, were co-bombarded with plasmid DNA containing cucumber mosaic virus (CMV) replicase under the rice actin (Act1) promoter and either the bar or PAT genes under the cauliflower mosaic virus (CaMV 355) promoter. Ten regenerated plants contained the transgene as analyzed by PCR, and two of the ten plants were confirmed to contain the transgene by Southern hybridization. The two transgenic plants were independent transformants, one containing the bar gene and the other both the CMV replicase and bar genes. Plants were sprayed at the rosette stage and found to be resistant to 1000 mg/L of phosphinothricin (Trade name-Ignite) indicating expression of the bar gene throughout the leaves when bar was under control of the CaMV 35S promoter.

• Korean journal of applied entomology
• /
• v.16 no.2 s.31
• /
• pp.133-137
• /
• 1977

Easter lily (Lilium longiflorum Thunb) was newly found infested with Aphelenchoides ritzemabosi at vinyl house of agricultural college, Kyungpook National University, Daegu, Korea in 1976. The symptoms of the infested plants are yellow to bronze leaves which become brown but not drop and the stem become stunted, fail to produce flowerw. The plants occured dieback symptoms and died eventually. Morphological characteristics are described.

• The Plant Pathology Journal
• /
• v.16 no.6
• /
• pp.306-311
• /
• 2000

A new strain of Cucumber mosaic virus (CMV) from easter lily (Lilium longiflorum), Ly2-CMV, was identified and compared to the well-characterized Mf-CMV (subgroupⅠ) and LS-CMV (subgroupⅡ) by host reaction in several indicator plants, dsRNA analysis, serological property, RT-PCR analysis, restriction enzyme profile of the PCR products and nucleotide sequence of coat protein (CP) gene. Remarkable differences in symptoms of Ly2-CMV were found between Mf-CMV or LS-CMV in tobacco plants and Datura stramoinium. Ly2-CMV induced small necrotic ringspots on the inoculated leaves of Nicotiana tabacum cvs. Xanthi nc and Burley 21 and D. stramonium, and failed to infect these species systemically. Of the indicator plants tested, N. benthamiana only reacted with systemic infection by inoculation of Lr2-CMV. In experiments of dsRNA analysis, serology and RT-PCR of CP gene, Ly2-CMV was come within subgroupⅠ CMV. However, restriction enzyme analysis of the PCR products using MspⅠ showed that Ly2-CMV was distinct to Mf-CMV. The CP gene of Ly2-CMV contains 657 nucleotides, and the nucleotide sequence is similar to that of Mf-CMV. There is also a high degree of conservation between their putative gene products in Ly2-CMV and Mf-CMV, with five amino acid changes in the 218 amino acids of the CPs.