• Title/Summary/Keyword: EapC

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Microcosm Study for Bioremediation of Oil-Contaminated Pebble Environments (자갈로 구성된 미소환경에서 미생물제제에 의한 유류분해)

  • Sim, Doo-Suep;Sohn, Jae-Hak;Kim, Sang-Jin
    • Korean Journal of Microbiology
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    • v.34 no.3
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    • pp.101-107
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    • 1998
  • Biological treatment of Arabian light crude oil-contaminated pebble was investigated in laboratory microcosms after supplementation with inorganic nutrients and oil-degrading microorganisms. Glass columns ($10cm{\times}20cm$) were used as microcosms and each microcosm was filled with pebbles of diameter less than 40 mm. After initial oil contamination of 2.4% (w/v), Inipol EAP-22 or slow release fertilizer (SRF) was added as inorganic nutrients and microorganisms were sprayed over pebbles. When $C_{17}$/pristane and $C_{18}$/phytane ratios were used as a marker for oil biodegradation, both ratios for microcosm supplemented with SRF and microorganisms were the lowest (below detectable range) after 92 days. Elimination of oil by abiotic processes, however, were minimal with decrease of $C_{17}$/pristane and $C_{18}$/phytane ratios from 3.55 and 2.41 to 3.06 and 1.50, respectively. The numbers of heterotrophic and oil-degrading microorganisms, and biological activity (dehydrogenase activity) corresponded to the course of biodegradation activities in all microcosms. During the whole experimental period, there was no significant nutrient deficiency only in the microcosm with SRF and microorganisms. It seemed that a continuous supply of inorganic nutrients using SRF was the most important factor for the successful performance of biological treatment in oil-contaminated pebbles.

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Active Authentication Method using NFC (NFC를 활용한 능동형 인증 방법)

  • Lee, Min-Gu;Kim, Dong-Wan;Sohn, Jin-Soo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37 no.2C
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    • pp.140-156
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    • 2012
  • Since most of recently launched smart devices support NFC(Near Field Communication), RFID applications are tend to be replaced. For instance, previous RFID application areas such as entrance control, mobile e-ticket, electronic payment and et. al are subject to change using NFC. Due to the limitation of passive communication in RFID, it is impossible to cover all security requirements of authentication and authorization mechanism that wide areas of applications demand. Therefore authentication and authorization mechanism based on NFC is very attractive to such applications because active communication methods make it possible to be highly secure in authentication and authorization. In this paper, authors propose a new approach of secure authentication and authorization mechanism using NFC smart devices based on EAP(Extensible Authentication Protocol) and AAA(Authentication, Authorization and Accounting) protocols.

Isolation, Purification, and Characterization of the Lytic Enzyme of Anabaena cylindrica by Penicillium oxalicum (HCLF-34) (Penicillium oxalicum(HCLF-34)으로부터 남조세균 (Anabaena cylindrica) 분해효소의 분리 및 동정)

  • 현성희;이호용;최영길
    • Korean Journal of Microbiology
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    • v.36 no.1
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    • pp.14-19
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    • 2000
  • Algal lytic enzyme, an extracellular enzyme, was purified from the culture filtrate of Penicillium oxalicum(HCLF-34) by ultrafiltration, gel filtration chromatography, and anion exchange chromatography. The enzyme has a molecular mass of approximately 22 kDa, an it is a monomer by renaturation SDS-PAGE. The amino acid sequences of the enzyme was revealed to be NH2-Glu-Ser-Tyr-Ser-Ser-Asn-Ala-Ala-Gly-Ala-Val-Leu-Ile---, had about 84% identity with the mature light chain of aspergillopepsin II precursor and 81% identity with the mature protein of the acid proteinase EapC precursor.

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Validity and Reliability of the Employee's Stress Response Scale (직장인 스트레스 반응 척도 개발 및 타당화 연구)

  • Lee, Jeong Eun;Choi, Bora;Jung, Young-Eun;Song, Gary;Kang, Min Jae;Chae, Jeong-Ho
    • Anxiety and mood
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    • v.8 no.2
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    • pp.153-160
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    • 2012
  • Objectives : The purpose of this study was to develop an employee's stress response scale (ESRS) and examine its validity and reliability. Methods : The study was conducted as follows : item generation, and test of validity and reliability. Items were developed via literature review, review of instruments, and data acquired from email-survey of counselors in the field of Employee Assistance Program (EAP). In order to test validity and reliability, data were collected from 400 employees. Results : The result of exploratory factor analysis of ESRS suggested 4 factor structures (work-related, anger, somatization, depression & anxiety) with a total of 25 items. ESRS showed a relatively strong positive correlation with Hospital Anxiety-Depression scale (HAD), Perceived Stress Scale (PSS), and Psychological Wellbeing Index (PWI), which showed ESRS had high convergent validity. Cronbach's ${\alpha}$ and test-retest reliability was .95 and .610 respectively. Conclusion : The ESRS is reliable and valid brief scale for measuring stress responses of employees. This scale would be useful in quantitatively evaluating job stress in workplace.

EAP를 이용한 청정 에너지 수확 기술 개발

  • Kim, S.H.;Lee, S.J.;Yoon, D.H.;Yang, Y.S.;Moon, J.H.;Lim, S.C.;Park, J.A.;Kim, Z.S.
    • Electronics and Telecommunications Trends
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    • v.23 no.6
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    • pp.22-31
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    • 2008
  • 화석연료를 개발함에 따라 인류는 과거보다 풍요한 물질문명을 누리게 되었으나 그 부작용으로 지구 온난화라는 현실적 재난에 직면해 있다. 점점 뜨거워지는 지구를 식히기 위해 세계적으로 당장 시급한 대책은 화석연료의 생산, 수송 및 소비에 있어서 효율을 높이고, 에너지를 절약함으로써 온실가스를 감축하는 것이다. 하지만 궁극적으로는 신재생 에너지와 같이 온실가스를 배출하지 않는 지속 가능한 에너지원을 사용함으로써 청정한 미래형 산업 시스템이 이루어야 한다. 에너지의 해외의존도가 97%에 육박하고 있는 우리나라의 입장에서 세계적으로 연평균 전력발전량의 약 20%를 담당하고있는 수력, 풍력, 조력 발전은 에너지 자급에 의한 수급 안정성 확보 측면에서 중요한 의미를 가진다. 특히, 화력 및 원자력과 비교할 때 환경에 대한 영향이 거의 없는 청정한 에너지로서 개발이 유망한 에너지이다. 대규모 수력발전은 대형 댐의 건설로 인한 환경.생태적 문제점이 있을 수 있으나, 풍력과 조력발전은 상대적으로 환경에 미치는 영향이 작은 편이다. 본 보고서에서는 최근 연구되고 있는 신재생 에너지 중 전기활성 고분자(EAP)를 이용하여 조력이나 유력, 풍력을 이용하여 청정 에너지를 생산할 수 있는 방법에 대하여 논하고자 한다.

Experimental Study of Collapse Delay Effect of Riprap on Dam Slope (사력댐 사석 보호공의 붕괴 지연 효과에 대한 실험 연구)

  • Jeong, Seokil;Kim, Seung Wook;Kim, Hong Taek;Lee, Seung Oh
    • Journal of Korean Society of Disaster and Security
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    • v.11 no.1
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    • pp.31-38
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    • 2018
  • The 99.1% of small dam and most of the levees in Korea are soil dam which can be constructed with lower cost and less effort compared with ones made of concrete. However, they are so vulnerable to overflow. Sudden collapses of these strucrues lead to increase flow rate rapidly, which may cause catastrophic problems in downstream regions. In this study, the experimental study on the collapse delay effect of riprap that was laid on slope of soil levee was carried out. A prismatic rectangular open channel was used and three different sizes of the riprap were installed on slope of a scaled earth dam. A new formula for the collapse time of the levee with the installation of riprap was presented, using the previous researches and the dimensional analysis. In this process, an unsteady flow condition was considered to derive the deviation time of the riprap. And additional experiments were conducted to understand the effect of reinforcement of riprap, and it was found that the reinforcement of riprap was more effective than twice sizing of intial riprap. If the collapse time is delayed, EAP (Emergency Action Plan) and forecasting can greatly reduce the degree of flood damage. Also, it will be meaningful that the results of this study are used for river design.

Evaluation of Fertilizer Additions to Stimulate Oil Biodegradation in Sand Seashore Mesocosms

  • CHOI, SUNG-CHAN;KAE KYOUNG KWON;JAE HAK SOHN;SANG-JIN KIM
    • Journal of Microbiology and Biotechnology
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    • v.12 no.3
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    • pp.431-436
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    • 2002
  • Effects of fertilizer additions for oil degradation were examined in sand seashore mesocosms. Within 37 days, up to $85\%$ removal was achieved by the addition of slow-release type fertilizer (SRF) with the initial degradation rate of 423.3 mg oil $(kg sand)^-1\;day^-1$. The removal was mostly of biological origin based on the changes of $C_17$ /pristane and $C_18$/phytane ratios from 2.60 to 0.81 and from 3.55 to 1.29, respectively. The addition of oleophilic fertilizer (Inipol EAP22) was less effective and resulted in the removal of $64\%$ of the added oil ($3\%$, v/v) with a lower initial degradation rate. Petroleum-degrading bacteria had achieved a value of $1{\times}10^8$ CFU $(g sand)^-1$ at Day 3 and this peak exactly coincided with the initial degradation in the SRF-treated mesocosm. In this mesocosm, surface tension values were decreased drastically during Days 3 and 8, suggesting that microbially-produced surface-active agents actively enhanced the oil degradation rate and cell proliferation. Although the Inipol-treated mesocosm appeared to show significantly enhanced oil degradation compared to that of the untreated control mesocosm, Inipol was found to be less effective than SRF in enhancing a true oil-degrader when compared under similar experimental conditions.

Biodegradation of Pyrene in Marine Environment (해양환경에서 Pyrene의 생분해)

  • 황순석;송홍규
    • Korean Journal of Microbiology
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    • v.35 no.1
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    • pp.53-60
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    • 1999
  • The biodegradation of recalcitrant polycyclic aromatic hydrocarbon, pyrene was investigated in microcosm simulating the beach sand and seawater. The natural biodegradation rates of pyrene were between 30-2,200 ng/g(ml)/day in beach sand and seawater when the pyrenc loading rates were 100- 1,000 ppm at 5-$20^{\circ}C$. The effects of the inoculum size, pyrene concentralion, incubation temperature and surfactant addition were investigated in fertilized (Inipol EAP 22) samples. Generally the biodegradation in beach sand was higher than that in seawater. A mixed inoculum (Pseudomonus, Acinetobacter, Moruxella) showed the 3,120 nglglday of biodegradation rate in beach sand with 200 ppm pyrene, which was 7.8 times higher than the natural biodegradation rate. The highest transformation rate, 4,860 ng/g/day was obtained in the bioaugmented beach sand (1,000 ppm pyrene). The glucose and surfactant addition to enhance the removal have negatively influenced on the biodegradation of pyrene. In case ol surfactants, CMC (critical micell concentration) might bc the control factor for the biodegradation.

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