• Title/Summary/Keyword: EST Analysis

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An Electrochemical Analysis on the Alloy Deposition of 80Ni-20Fe Permalloy Thin Film (80Ni-20Fe Permalloy 박모 동시석출 기구에 관한 전기화학적 고찰)

  • Lee, K. H.;Kang, Tak;Ra, H. Y.
    • Journal of the Korean institute of surface engineering
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    • v.15 no.1
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    • pp.11-18
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    • 1982
  • 80% Ni-Permalloy is soft magnetic material with high initial permeability and low magnetic coercive force Hc, and is used to computer memory cores and minirelays of communication e-ngineering. In this paper 80 Permalloy thin film on copper cathode was alloy-deposited from Watts so-lution contatining FeSO4$.$7H2O. The amount of FeSO4$.$7H2O in the solution, pH, temperature of the solution and plating current density were varied as parameters and the resulting comp-osition changes of deposited film were analyzed electrochemically with respect to the parame-ters. From the above procedure electroplating conditions for deposition of 80 Permalloy were est-ablished as following: 17-21 g/$\ell$ of FeSO4$.$7H2O in Watts solution, current density 1.0-2.0 Amp/dm2, pH 2.5-3.0 and temperature range of 50-60$^{\circ}C$.

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An analysis of determining factor for frozen shoulder patients diagnosis and treatment of physical therapists (동결견 환자에 대한 물리치료사의 진단 및 치료방법 결정기준)

  • Oh, Young-Taek;Kim, Ki-Won;Kwon, Hyuk-Cheol
    • Journal of Korean Physical Therapy Science
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    • v.5 no.4
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    • pp.777-784
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    • 1998
  • The purpose of this study was to determine the diagnosis and treatment patterns for frozen shoulder patients. It was used Delphi survey techniques and the subjects were 35 expect physical therapists who agreed participated in the study. The data were analyzed by the x2-test at 0.05 level of statistical significance. The results were as follows : 1. In accordance with open ended question, 11 diagnosis and 14 treatment are collected in the first investigation. 2. In accordance with close ended question, they were selected 5 diagnosis and 5 treatments criteria from the results of the first replied questionnaire. 3. Finally, the third close ended questionnaire, they were selected 3 main driteria of the diagnosis and treatments from the results of the second investigation. The physical therapists prefer to 1) History 2) ROM test 3) Palpation & Inspection in the diagnostic field, and 1) EST and Convex-Concave rule 2) Codman's exercise and MFR 3) Deep friction massage in the treatment field.

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Molecular Cloning of a Cellulase Gene from Abalone Haliotis discus hannai and Its Expression in E coli

  • Park, Eun-Mi;Han, Yun-Hee;Park, In-Suk;Nam, Bo-Hye;Kong, Hee Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Young-Ok
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.108-112
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    • 2007
  • A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

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An Experimental Study on the Pozzolan Reaction of discarded Bentonite by the Cooling Method after Heat Treatment (소성가공한 폐 벤토나이트 분말의 냉각방법에 따른 포졸란 반응성에 관한 실험적 연구)

  • Kim, Hyo-Yeul;Kang, Byeung-Hee
    • Journal of the Korea Institute of Building Construction
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    • v.2 no.3
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    • pp.139-146
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    • 2002
  • As the bentonite is main material to prevent from collapse of drilling hole at underground excavation works, it is increased using quantity on construction industry day by day. But, the discarded bentonite that is over using at underground excavation works is caused various environmental trouble as soil and water pollution est. This study aims to propose a foundamental report for pozzolan reaction of discarded Bentonite powder by heat-treatment and cooling as concrete mineral admixture. To find out pozzolan reaction ability of discarded Bentonite powder by indirect cooling & cooling using of water after heat-treatment, the experiments are excuted Phenolphtalein test, setting test, pH test and the analysis by X-ray diffractor. As a result of this study, discarded Bentonite powder can be utilized as concrete mineral admixture by heat-treatment and especially, pozzolan reaction ability of discarded Bentonite powder is superior to the situation of 50$0^{\circ}C$~$700^{\circ}C$, 60min.

Gene Expression in the Muscles of young and Mature Channel Catfish (Ictalurus punctatus) as Analyzed by Expressed Sequence Tags and Gene Filters

  • Soon-Hag Kim
    • Journal of Aquaculture
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    • v.16 no.1
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    • pp.8-14
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    • 2003
  • To generate expressed sequence tags for genomics research involving genetic linkage analysis, to examine gene expression profiles in muscles of channel catfish in a non-normalized muscle cDNA library, to compare gene expression in young and mature channel catfish muscles using the EST reagents and gene filters to demonstrate the feasibility of functional genomics research in small laboratories. 102 randomly picked cDNA clones were analyzed from the catfish muscle cDNA library. Of the sequences generated, 90.2% of ESTs was identified as known genes by identity comparisons. These 92 clones of known gene products represent transcriptional products of 24 genes. The 10 clones of unknown gene products represent 8 genes. The major transcripts (70.1% of the analyzed ESTs) in the catfish muscle are from many major genes involved in muscle contraction, relaxation, energy metabolism and calcium binding such as alpha actin, creatine kinase, parvalbumin, myosin, troponins, and tropomyosins. Gene expression of the unique ESTs was comparatively studied in the young and adult catfish muscles. Significant differences were observed for aldolase, myostatin, myosin light chain, parvalbumin, and an unknown gene. While myosin light chain and an unknown gene (CM 192) are down-regulated in the mature fish muscle, the aldolase, myostatin, and parvalbumin are significantly up-regulated in the mature fish muscle. Although the physiological significance of the changes in expression levels needs to be further addressed, this research demonstrates the feasibility and power of functional genomics in channel catfish. Channel catfish muscle gene expression profiles provide a valuable molecular muscle physiology blueprint for functional comparative genomics.

Study on the Seasonal and Regional Variation of Rutin Content of Leonuri Herba (익모초의 계절 및 지역별 Rutin 함량변화 연구)

  • Lee, Seong-Wan;Kim, Gi-Eun;Chung, Sung-Hee;Kim, Sung-Gun;Kim, Do-Hoon;Kim, Jin-Hyo;Kim, Ho-Kyoung;Whang, Wan-Kyunn
    • YAKHAK HOEJI
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    • v.51 no.3
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    • pp.157-163
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    • 2007
  • In a pattern analysis of GC-MS of 21 Leonuri herba samples harvested in China and Korea, characteristic differences were observed between Chinese and Korean samples. specifically, Leonuri herba form Hanllasan showed unusual retention time. Rutin was employed as a standard compound of Leonuri herba. All samples had 0.32${\pm}$0.18(%) rutin contents in average, but Korean samples had more rutin than Chinese one, especially the sample from Hanllasan of Jeju showed high-est rutin content (3.2%). In rutin contents of seasonal variation, there were more rutin contents in after blooming, 2years aged Leonuri herba than before.

Mapping of Quantitative Trait Loci for Salt Tolerance at the Seedling Stage in Rice

  • Lee, Seung Yeob;Ahn, Jeong Ho;Cha, Young Soon;Yun, Doh Won;Lee, Myung Chul;Ko, Jong Cheol;Lee, Kyu Seong;Eun, Moo Young
    • Molecules and Cells
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    • v.21 no.2
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    • pp.192-196
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    • 2006
  • Salt tolerance was evaluated at the young seedling stage of rice (Oryza sativa L.) using recombinant inbred lines (MG RILs) from a cross between Milyang 23 (japonica/indica) and Gihobyeo (japonica). 22 of 164 MG RILs were classified as tolerant with visual scores of 3.5-5.0 in 0.7% NaCl. Interval mapping of QTLs related to salt tolerance was conducted on the basis of the visual scores at the young seedling stage. Two QTLs, qST1 and qST3, conferring salt tolerance, were detected on chromosome 1 and 3, respectively, and the total phenotypic variance explained by the two QTLs was 36.9% in the MG RIL population. qST1 was the major QTL explaining 27.8% of the total phenotypic variation. qST1 was flanked by Est12~RZ569A, and qST3 was flanked by RG179~RZ596. The detection of new QTLs associated with salt tolerance will provide important information for the functional analysis of rice salt tolerance.

Properties of SiOCH Thin Film Lour Dielectric by BTMSM/O2 Flow Rates (BTMSM/O2 유량변화에 따른 SiOCH 박막의 저유전 특성)

  • Park, In-Cheol;Kim, Hong-Bae
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.22 no.2
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    • pp.132-136
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    • 2009
  • SiOC thin film of hybrid-type that is the limelight as low dielectric material of next generation were deposited by plasma enhanced chemical vapor deposition (PECVD) method with bistrimethylsilylmethane (BTMSM) precursor increased by 2 sccms from 24 sccms to 32 sccm. Manufactured samples are analyzed components by measuring FT/IR absorption lines. It is a tendency that seems to be growing of Si-O-Si(C) bonding group and narrowing of Si-O-$CH_3$ bonding group relative to the increasing flow-rate BTMSM. The chemical shift in the XPS analysis was shown in the specimens between the BTMSM=26 sccm and BTMSM = 28 sccm. The binding energy of Si 2p, C 1s and O 1s electron orbit spectra was the low-est at the specimen of the BTMSM=26 sccm. From the results of electrical Properties using the 1 MHz C - V measurements, the dielectric constant was 2.32 at the specimen with the BTMSM = 26 sccm.

Detoxification Mechanism and Isoenzyme Pattern Changes against Cadmium in Rhizopus oryzae (Rhizopus oryzae의 카드뮴 해독기작과 이에 관련된 동위효소의 변화 양상)

  • Lee, Ki-Sung;Kim, Young-Ho;Park, Young-Sik;Park, Yong-Keun
    • The Korean Journal of Mycology
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    • v.23 no.1 s.72
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    • pp.86-91
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    • 1995
  • Isoenzymatic analysis related with cadmium adaptation and detoxifying mechanism were carried out upon Rhizopus oryzae. When cadmium was added into R. oryzae culture, activities of malate dehydrogenase (MDH) and glucose phosphate isomerase (GPI) related with carbohydrate metabolizing pathways were stimulated. Novel isoenzyme CAT-2 related with removing intracellular toxic peroxides, was induced lately and derepressed very highly. On the other hand, lactate-catabolizing enzymes such as lactate dehydrogenase (LDH) and alcohol dehydrogenase (ADH) were repressed. These results strongly suggest that, under cadmium stress, much of derepression of enzymes relating with central metabolism such as TCA cycle that produces high yield of energy and relating with removal of toxic peroxides should be necessary.

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In silico analysis of candidate genes involved in light sensing and signal transduction pathways in soybean

  • Quecini, V.;Zucchi, M.I.;Pinheiro, J.B.;Vello, N.A.
    • Plant Biotechnology Reports
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    • v.2 no.1
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    • pp.59-73
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    • 2008
  • Several aspects of photoperception and light signal transduction have been elucidated by studies with model plants. However, the information available for economically important crops, such as Fabaceae species, is scarce. In order to incorporate the existing genomic tools into a strategy to advance soybean research, we have investigated publicly available expressed sequence tag (EST) sequence databases in order to identify Glycine max sequences related to genes involved in light-regulated developmental control in model plants. Approximately 38,000 sequences from open-access databases were investigated, and all bona fide and putative photoreceptor gene families were found in soybean sequence databases. We have identified G. max orthologs for several families of transcriptional regulators and cytoplasmic proteins mediating photoreceptor-induced responses, although some important Arabidopsis phytochrome-signaling components are absent. Moreover, soybean and Arabidopsis genefamily homologs appear to have undergone a distinct expansion process in some cases. We propose a working model of light perception, signal transduction and response-eliciting in G. max, based on the identified key components from Arabidopsis. These results demonstrate the power of comparative genomics between model systems and crop species to elucidate several aspects of plant physiology and metabolism.