• Title/Summary/Keyword: ESB

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Inductions of Caspase-, MAPK- and ROS-dependent Apoptosis and Chemotherapeutic Effects Caused by an Ethanol Extract of Scutellaria barbata D. Don in Human Gastric Adenocarcinoma Cells

  • Shim, Ji Hwan;Gim, Huijin;Lee, Soojin;Kim, Byung Joo
    • Journal of Pharmacopuncture
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    • v.19 no.2
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    • pp.129-136
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    • 2016
  • Objectives: The crude extracts of Scutellaria barbata D. Don (SB) have traditionally demonstrated inhibitory effects on numerous human cancers both in vitro and in vivo. Gastric cancer is one of the most common types of cancer on world. The authors investigated the effects of an ethanol extract of Scutellaria barbata D. Don (ESB) on the growth and survival of MKN-45 cells (a human gastric adenocarcinoma cell line). Methods: The MKN-45 cells were treated with different concentrations of ESB, and cell death was examined using an MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay. Analyses of sub-G1 peaks, caspase-3 and -9 activities, and mitochondrial membrane depolarizations were conducted to determine the anti-cancer effects of SB on MKN-45 cells. Also, intracellular reactive oxygen species (ROS) generation was investigated. Results: ESB inhibited the growth of MKN-45 cells, caused cell cycle arrest, and increased the sub-G1 population. In addition, ESB markedly increased mitochondrial membrane depolarization and the activities of caspase-3 and -9. ESB exerted anti-proliferative effects on MKN-45 cells by modulating the mitogen-activated protein kinase (MAPK) signaling pathway and by increasing the generation of ROS. Furthermore, combinations of anti-cancer drugs plus ESB suppressed cell growth more than treatments with an agent or ESB, and this was especially true for cisplatin, etoposide, and doxorubicin. Conclusion: ESB has a dose-dependent cytotoxic effect on MKN-45 cells and this is closely associated with the induction of apoptosis. ESB-induced apoptosis is mediated by mitochondria-, caspase- and MAPK dependent pathways. In addition, ESB enhances ROS generation and increases the chemosensitivity of MKN-45 cells. These results suggest that treatment with ESB can inhibit the proliferation and promote the apoptosis of human gastric adenocarcinoma cells by modulating the caspase-, MAPK- and ROS-dependent pathway.

Design and Implementation of Lightweight ESBus Engine for Service Oriented Architecture (서비스 지향 아키텍처를 위한 경량 ESB 엔진의 설계 및 구현)

  • Kim, Yoon-Ho;Cho, Seong-Hwan
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.14 no.6
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    • pp.131-137
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    • 2014
  • Service Oriented Architecture(SOA) is a flexible structure which does not affect other services even when a specific service is changed. It provides the platform with neutral interfaces independent of any others, where services are loosely coupled in a standard way. While ESB (Enterprise Service Bus) is a key technology for service-oriented architecture, few research and development for ESB has been done. In this paper, we designed and implemented the key components of ESB such as mediation service, message oriented middleware service, operation service, monitoring service, application connection service, and database connection service. The performance evaluation was done by measuring the message processing time for the number of messages with various sizes.

Root Extract of Scutellaria Baicalensis Increases Gefitinib Sensitivity in H1975 Human Non-small Cell Lung Cancer Cells (H1975 세포에서 황금추출물에 의한 gefitinib 저항성 억제 효과)

  • Park, Shin-Hyung;Park, Hyun-Ji
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.35 no.4
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    • pp.117-123
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    • 2021
  • Gefitinib, a first generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR TKI), provides obvious clinical benefit in patients with EGFR-mutant non-small cell lung cancer (NSCLC). However, patients ultimately develop gefitinib resistance which mainly caused by EGFR T790M secondary mutation. In the current study, we investigated whether the root extract of Scutellaria baicalensis (SB) overcomes gefitinib resistance. Gefitinib-resistant H1975 human NSCLC cells (EGFR L858R/T790M double mutant) were treated with gefitinib and/or ethanol extract of SB (ESB) to evaluate the effect of ESB on the gefitinib sensitivity. The cell viability was measured by MTT assay and trypan blue exclusion assay. The colony-forming ability was evaluated by anchorage-dependent colony formation assay. Combined treatment with gefitinib and ESB markedly decreased the cell viability and colony formation than single treatment with gefitinib or ESB in H1975 cells. In addition, cells treated with both gefitinib and ESB exhibited a significant increase of sub-G1 DNA content which indicates apoptotic cells compared with those treated with gefitinib or ESB alone. As a molecular mechanism, combined treatment with gefitinib and ESB strongly downregulated the phosphorylation of ERK and JNK than single treatment with gefitinib or ESB. Taken together, our results demonstrate that ESB sensitizes H1975 cells to gefitinib treatment. We cautiously propose that ESB can be used in combination with gefitinib for the advanced NSCLC patients with acquired resistance to EGFR TKIs.

A Study on Environmental Standards of School Building (교사환경기준에 관한 연구)

  • Hong, Seok-Pyo;Park, Young-Soo
    • The Journal of Korean Society for School & Community Health Education
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    • v.1 no.1
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    • pp.11-43
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    • 2000
  • The purpose of this study was, through analyzing the previous researches, to grasp the present status of environment of school building(ESB), research the sundry records of each element and, through comparative analysis of the standard of ESB in Korea, the United States, and Japan, select the normative standard of ESB, to clarify the point at issue presented in Regulation of Construction & facility Management for Elementary and and Secondary School in Korea, and to suggest an alternative preliminary standard of ESB. To carry out a research for this purpose, these were required: 1. to investigate the existing present status of ESB, 2. to make a comparative analysis of the standard of ESB in each country, 3. to suggest the normative standard of preliminary standard of ESB, 4. to analyze the controversial points of the standard of ESB in Korea, 5. to suggest an alternative preliminary standard of ESB. The conclusions were as follows: 1. Putting, through analyzing the previous researches, the existing present status of ESB together, it seemed that lighting environment, indoor air environment and noise environment were all in poor conditions. 2. In the result of a comparative analysis of the standard of ESB in Korea, Japan and the United States, in Korea the factors of each lighting and indoor air environment were not presented properly, in Japan, in lighting environment aspect, the standard on natural lighting and the factors on brightness were not presented., and in the USA the essential factors of each environment were throughly presented. In the comparison of the standards on each factor, Korea showed that the standard level presented was less properly prescribed than those of the USA and Japan but it also showed that the standard levels prescribed in the USA and in Japan were mostly similar to the standard levels in records investigated. 3. With the result of the normative standard selection on School Builiding environment factor of prescribed in this study, the controversial points of the standard of ESB in Korea were analyzed and the result was utilized to suggest new preliminary standard of ESB. 4. As the result of the analysis of the controversial points of the standard of ESB in Korea, it was found that the standard of ESB in Korea should be established on a basis of School Health Act and be concretely presented in School Health Regulation and School Health Rule. The factors of each environment was improperly presented in the existing standard of ESB in Korea. Moreover the standard of them was inferior to that of the records investigated and those of in the USA and in Japan and it also showed that the standard of it in Korea was improper to maintain Comfortable Learning Environment. 5. A suggested preliminary standard of ESB acquired through above study as follows: 1) In this study a new kind of preliminary standard of ESB is divided into lighting environment, indoor air environment, noise environment, odor environment and for above classification, reasonable factor and standard should be established and the controling way on each standard and countermeasures against it should be considered. 2) In lighting environment, the factors of natural lighting are divided into daylight rate, brightness, glare. In the standard on each factor, daylight rate should secure 5% of a mean daylight rate and 2% of a minimum daylight rate, brightness ratio of maximum illumination to minimum illumination should be under 10:1, and in glare there should not be an occurrence factor from a reflector outside of the classroom. And the factors of unnatural lighting are illumination, brightness, and glare. In the standard on each factor, illumination should be 750 lux or more, brightness ratio should be under 3 to 1, and glare should not occur. And Optimal reflection rate(%) of Colors and Facilities of Classroom which influences lighting environment should be considered. 3) In indoor air environment factors, thermal factors are divided into (1) room temperature, (2) relative humidity, (3) room air movement, (4) radiation heat, and harmful gases (5) CO, (6) $CO_2$ that are proceeded from using the heating fuel such as oval briquettes, firewood, charcoal being used in most of the classroom, and finally (7) dust. In the standard on each factor, the next are necessary; room temperature: $16^{\circ}C{\sim}26^{\circ}C$(summer : $E.T18.9{\sim}23.8^{\circ}C$, winter: $E.T16.7{\sim}21.7^{\circ}C$), relative humidity: $30{\sim}80%$, room air movement: under 0.5m/sec, radiation heat: under $5^{\circ}C$ gap between dry-bulb temperature and wet-bulb temperature, below 1000 ppm of ca and below 10ppm of $CO_2$, dust: below 0.10 $mg/m^3$ of Volume of dust in indoor air, and ventilation standard($CO_2$) for purification of indoor air : once/6 min.(about 7 times/40 min.) in an airtight classroom. 4) In the standard on noise environment, noise level should be under 40 dB(A) and the noise measuring way and the countermeasures against it should be considered. 5) In the standard on odor environment, odor level under Physical Method should be under 2 degrees, and the inspecting way and the countermeasures against it should be considered.

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Validation of Equivalent Shear Beam Container Using Dynamic Centrifuge Tests (동적 원심모형실험을 이용한 등가전단보 토조의 성능 검증)

  • Kim, Yoon-Ah;Lee, Hae-In;Ko, Kil-Wan;Kim, Dong-Soo
    • Journal of the Korean Geotechnical Society
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    • v.36 no.11
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    • pp.61-70
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    • 2020
  • In dynamic centrifuge tests, equivalent shear beam (ESB) container minimizes the boundary effect between the soil model and the wall of the container so as to effectively simulate the boundary conditions of real field state. The ESB container at KAIST was evaluated to be performing properly by Lee et al. (2013). However, it is necessary to re-evaluate the performance of ESB container since the ESB container may have deteriorated over time. Thus, the performance of eight-year-old ESB container was re-evaluated through dynamic centrifuge tests. Firstly, the natural period of the empty ESB container was compared with the results of Lee et al. (2013). Then the boundary effect of sand-filled ESB container was evaluated. Results show that the dynamic behavior of the sand-filled ESB container was similar to that of the ground, despite a decrease in the natural period of the empty ESB container over time. In addition, the dynamic response of the ground built in the ESB container and the same ground simulated through numerical analysis with free-field boundary conditions were similar. Therefore, it was found that the boundary effect of the ESB container due to the decrease in the natural period was not significant.

Milk Conjugated Linoleic Acid (CLA) Profile and Metabolic Responses of Dairy Cows Fed with High-temperature-micro-time (HTMT) Treated Diets Containing High Quantity Extruded Soybean (ESB)

  • Lee, H.G.;Hong, Z.S.;Wang, J.H.;Xu, C.X.;Jin, Y.C.;Kim, T.K.;Kim, Y.J.;Song, M.K.;Choi, Yun.-Jaei
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.11
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    • pp.1504-1512
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    • 2009
  • A feeding trial was conducted to examine the effect of high-temperature-micro-time (HTMT) processing of diets containing extruded soybean (ESB) in high quantity on milk fat production, metabolic responses, and the formation of conjugated linoleic acid (CLA) and trans-vaccenic acid (TVA). Twenty-one multiparous Holstein cows in mid-lactation were blocked according to milk yield in the previous lactation. Cows within each block were randomly assigned to either normal concentrate or HTMT treated diets containing ESB (7.5% HTMT-ESB and 15% HTMT-ESB). It was hypothesized that the HTMT-ESB would affect the undegradable fatty acids in the rumen and, thus, would modify the fatty acid profile of milk fat. Both 7.5% and 15% HTMT-ESB did not affect milk yield, fat, protein, lactose and solid-not-fat (SNF), but the proportion of cis-9, trans-11 CLA in milk fat was significantly increased by these treatments. Content of TVA in milk fat was not affected by HTMT-ESB. The HTMT-ESB influenced the fatty acid profile in milk fat, but there was little difference between 7.5% and 15% of supplementation. HTMT-ESB feeding significantly decreased the concentration of plasma insulin and glucose, while plasma growth hormone (GH), triglyceride (TG), non-esterified fatty acid (NEFA) and HDLcholesterol were increased by 7.5% and 15% ESB-HTMT supplementation in comparison to the control group (p<0.05). However, no significant difference was observed in plasma LDL-cholesterol, insulin like growth factor (IGF)-1, T3, T4, and leptin concentrations among treatments (p>0.05). The present results showed that cis-9, trans-11 CLA production was increased by HTMT treatment of dietary ESB without reduction of milk fat, and the unchanged milk fat and yield was assumed to be associated with the constant level of thyroid hormones, leptin, and IGF-1.

Developing Service for Managing Data Quality on ESB (ESB 상에서 데이터 품질 관리를 위한 서비스 개발)

  • 문은영;최병주;이정원
    • Proceedings of the Korean Information Science Society Conference
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    • 2004.10b
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    • pp.517-519
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    • 2004
  • 기존의 컴포넌트 기반으로 응용 프로그램을 개발하던 소프트웨어 개발 방법론이 분산 컴퓨팅 환경에서 대규모 시스템 통합의 요구가 생겨남에 따라 서비스-기반 구조(SOA: Service-Oriented Architecture)로 점차 변화하고 있다. SOA를 구현하기 위한 최신 통합 기술인 엔터프라이즈 서비스 버스(ESB: Enterprise Service Bus)상에서 상호 작용하는 시스템 간에 교환되는 데이터 품질을 보장할 수 있다면 더 나은 서비스를 제공할 수 있다. 본 논문은 기존에 개발된 DAQUM(Data Quality Measuring tool)(1)을 이용하여 ESB상에서 통합된 시스템들간의 상호 작용하는 데이터 품질을 보장하기 위한 서비스를 개발한다.

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Revision of Ecological Score of Benthic Macroinvertebrates Community in Korea (한국의 저서성 대형무척추동물 생태점수 개선)

  • Kong, Dongsoo;Park, Youngjun;Jeon, Yong-Rak
    • Journal of Korean Society on Water Environment
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    • v.34 no.3
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    • pp.251-269
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    • 2018
  • In 1997, the environmental score (Q) corresponding the tolerance value of Korean benthic macroinvertebrate species and the related biotic score, the ecological score of benthic macroinvertebrates (ESB) were proposed; ESB was similar to Beck's biotic index or Chandler's biotic score. This study was carried out to revise the Q values of individual species and the assessment scheme of ESB based on the taxonomic performance and ecological information accumulated since then. The original ESB was renamed as TESB (total ESB), and AESB (average ESB) was newly proposed. AESB is calculated by dividing the TESB (for a given station) by the number of species present in the sample. In this study, TESB showed a positively skewed distribution, while AESB showed a negatively skewed distribution. The correlation between TESB and the concentration of $BOD_5$ was a little stronger than that of the original ESB. TESB showed a very strong correlation (correlation coefficient r = 0.98) with Margalef's species richness, of which correlation coefficient was higher than that of AESB (r = 0.85). AESB showed a strong correlation (r = -0.79) with the concentration of $BOD_5$, while TESB showed a weaker correlation (r = -0.67). Applying TESB and AESB together in an assessment of the environment may be comprehensive because the physical and chemical states of the environment can be evaluated together. AESB is less dependent on the sample size, while TESB tends to increase as the sample size increases. In the evaluation of the environment using TESB, it is necessary to standardize the methods on monitoring.

A Message Monitoring Framework for Tracing Messages on JBI-based Enterprise Service Bus (JBI 기반 ESB 환경에서 효과적인 메시지 추적을 위한 메시지모니터링 프레임워크)

  • Choi, Jae-Hyun;Park, Jae-Won;Lee, Nam-Yong
    • Journal of Information Technology Services
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    • v.9 no.2
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    • pp.179-192
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    • 2010
  • In order to resolve the problems of traditional Enterprise Application Integration (EAI) for system integration and to establish flexible enterprise IT environments, Enterprise Service Bus(ESB) which have distributed architecture and support Service Oriented Architecture(SOA) has introduced. Particularly, JBI which developed by the Java Community Process is most widely used to implement ESB for advantages of Java technology. In ESB based on JBI, reliable message delivery is very important to ensure stability of services and systems because it is a message driven architecture. But, it is difficult to verify messages and trace messages when system fault or service error occurred because JBI specification is not enough to address them. In this paper we has proposed the Message Monitoring Framework for JBI-based ESBs which for using in monitoring messages efficiently. It provides foundations for gathering and tracing message-related information about component installation, message exchange, service deploy by using proxy-based change tracking and delegation mechanism for data processing. The proxy which used in our solutions collects data about message automatically when it changed, and the delegation mechanism provides users flexibility for data processing. Also, we describe the performance evaluation results of our solution which is acceptable. We expect to it enables users to ensure reliability and stability of the JBI-based ESB by systematic monitoring and managing messages being used to interact among components.

A Study on Design Pattern Applied Message Monitoring System for Open Source Software based ESB (Enterprise Service Bus) (오픈 소스 기반 ESB의 패턴 적용 메시지 모니터링 시스템 구축 사례)

  • Lee, Sung-Jae
    • Proceedings of the Korea Information Processing Society Conference
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    • 2012.04a
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    • pp.1290-1293
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    • 2012
  • 오픈 소스 기반 ESB 모니터링 시스템 설계에 EIP(Enterprise Integration Pattern)을 적용하여 비즈니스 요구사항을 처리하는 통합 로직과 메시지 추적 관리를 위한 관리 로직을 분리하여, 메시지 관리 로직 처리에 따른 성능 저하 및 오류에 의한 영향도를 최소화하며 메시지 데이터를 중앙 저장 장치에 저장하여 메시지 추적 관리를 용이한 모니터링 시스템 구축 사례.