• Proceedings of the KSRS Conference
• /
• 2008.03a
• /
• pp.164-169
• /
• 2008

한국해양연구원에서 수신한 자료인 NOAA 12, 16, 17, 18호등의 Advanced Very High Resolution Radiometer(AVHRR) 센서자료와 국립조사원에서 제공하는 해양실측자료인 정선 관측 자료를 이용하여 두 가지의 알고리즘 적용을 통하여 비교 및 분석을 해보고자 한다. 연구 기간은 2006년 1월부터 4월 자료 중 구름의 영향이 없는 영상에서 실측자료와 동일한 날짜 총 107개의 정점 값을 추출하였다. 위성 자료에서 해수면 온도 추출방법은 split window 방법으로, 고정계수 값을 사용하는 linear algorithm(MCSST), nonlinear algorithm(NLMCSST)을 이용하였다. 연구 지역은 동해, 황해, 남해 지역에 대한 지역별로 두 알고리즘을 비교 적용하였다. 전 해역의 값을 이용하여 위성자료와 실측자료를 비교 분석한 결과 linear algorithm방법의 평균 오차 값은 0.71$^{\circ}C$이고 상관도는 1%이고, nonlinear 방법의 오차 값은 0.35$^{\circ}C$ 상관도는 1%로 나타났다. 해역별로는 linear한 알고리즘을 적용하여 동해는(ES)는 R=1, 오차 값은 0.37$^{\circ}C$ 황해(YS)는 R=0.99 오차 값은 0.125$^{\circ}C$ 남해(SS)는 R=0.99 오차 값은 1.2$^{\circ}C$보였다. nonlinear한 알고리즘을 적용하여 동해는(ES)는 R=1, 오차 값은 0.4$^{\circ}C$ 황해(YS)는 R=0.99 오차 값은 0.13$^{\circ}C$ 남해(SS)는 R=0.99 오차 값은 0.82$^{\circ}C$의 결과를 보여 주었다. 동해와 황해지역은 linear한 알고리즘을 적용한 결과가 실측자료와의 오차 값이 작았고, 남해지역은 linear한 알고리즘을 적용한 결과보다 nonlinear 알고리즘을 적용한 것이 작은 오차 값을 보여주었다. 이는 남해 해역의 자료가 대기의 상태나 다른 영향을 받아 해수면온도 값이 추정된 것으로 보여 진다. 해역별로 최적화된 알고리즘을 적용하여 해수면온도의 산출을 통해서 위성자료의 정밀도 지구환경변화 모니터링 등 많은 연구에 위성자료의 활용이 증대될 것으로 기대한다.

• Development and Reproduction
• /
• v.6 no.1
• /
• pp.55-66
• /
• 2002

Embryonic stem cells(ES cells) are derived from the inner cell mass(ICM) of blastocysts, which have the potentials to remain undifferentiated, to proliferate indefinitely in vitro, to differentiate into the derivates of three embryonic germ layers. ES cells are an attractive model system for studying the initial developmental decisions and their molecular mechanisms during embryogenesis. Additionally, ES cells of significant interest to those characterizing the various gene functions utilizing transgenic and gene targeting techniques. We investigated the effects of reproductive hormones, gonadotropins(GTH) and steroids on the induction of differentiation and expressions of their receptor genes using the newly established mouse ES cells. We collected the matured blastocysts of inbred mice C57BL/6J after superovulation and co-cultured with mitotically inactivated STO feeder cells. After 5 passages, we confirmed the expression alkaline phosphatase(Alk P) activity and SSEA-1, 3, 4 expressions. The protocol devised for inducing ES differentiation consisted of an aggregation steps, after 5 days as EBs in hormone treatments(FSH, LH, E$_2$, P$_4$, T) that allows complex signaling to occur between the cells and a dissociation step, induced differentiation through attachment culture during 7 days in hormone treatments. Hormone receptors were not increased in dose-dependent manner. All hormone receptors in ES cells treated reproductive hormones were expressed lower than those of undifferentiated ES cell except for LHR expression in E$_2$-treated ES cells group. After hormone induced differentiation, at least some of the cells are not terminally differentiated, as is evident from the expression of Oct-4, a marker of undifferentiated. To assess their differentiation by gene expression, we analyzed the expression of 7 tissue-specific markers from all three germ layers. Most of hormone-treated group increased in the expression of gata-4 and $\alpha$ -fetoprotein, suggesting reproductive hormone allowed or induced differentiation of endoderm.

• Development and Reproduction
• /
• v.16 no.4
• /
• pp.363-370
• /
• 2012

The outer dense fiber 2 (ODF2) protein is an important component of sperm tail outer dense fiber and localizes at the centrosome. It has been reported that the RO072 ES cell derived homozygote knock out of ODF2 results in an embryonic lethal phenotype, and XL169 ES cell derived heterozygote knock out causes severe defects in sperm tail development. The ODF2s splicing variant, Cenexin1, possesses a C-terminal extension, and the phosphorylation of serine 796 residue in an extended C-terminal is responsible for Plk1 binding. Cenexin1 assembles ninein and causes ciliogenesis in early stages of the cell cycle in a Plk1-independent manner. Alternatively, in the late stages of the cell cycle, G2/M phase, Cenexin1 binds to Plk1 and results in proper mitotic progression. In this study, to identify the in vivo function of Plk1 binding to phosphorylated Cenexin1 S796 residue, and to understand the in vivo functional differences between ODF2 and Cenexin1, we generated ODF2/Cenexin1 S796A/Cenexin1 WT expressing transgenic mice in a RO072 ES cell derived $ODF2^{+/-}$ knock out background. We observed a severe defect of sperm tail development by ectopic expression of Cenexin1 S796A mutant and no phenotypic differences between the ectopic expression of ODF2/Cenexin1 WT in $ODF2^{+/-}$ background and in normal wild type mice.

• Nutrition Research and Practice
• /
• v.6 no.5
• /
• pp.429-435
• /
• 2012

Atopic dermatitis (AD) has become a serious epidemic in Korean children. We aimed to investigate the association between vitamin C, E and other nutrients, and serum total IgE/specific IgE levels in children with AD. A total of 119 children (0-24 mo) diagnosed with AD were recruited for this cross-sectional study from a medical center in Seoul. A 24 h recall was used to assess dietary intakes. Serum total and six food-allergen specific IgE levels were measured by CAP-FEIA. Serum vitamin E was also measured but only in 25 out of the total 119 participants. Multiple linear regression analysis was performed to estimate the coefficients between serum IgE levels and dietary intake as well as serum vitamin E. Serum vitamin E levels showed a significantly inverse association with serum total IgE and all specific IgE levels (P < 0.05). Fat intake was inversely related with specific-IgEs for egg whites, milk, buck wheat, soy, and peanuts (P < 0.05). Positive associations were found between carbohydrate (CHO) intake and total IgE and specific IgEs to egg whites, milk, soy, and peanuts (P < 0.05). Vitamin C, E and n-3/n-6 fatty acids were not related with serum total IgE and specific IgE levels except for the association between buck wheat and vitamin E. In addition, there were no significant differences between males and females in dietary intake and serum IgE levels by student's t-test. Although dietary vitamin E showed no association with serum IgE levels, serum vitamin E drew a significant inverse relationship with serum IgE levels. The evidence seems to suggest that vitamin E may possibly lower total and specific-IgEs in children with AD, and that it is important to maintain a relatively high serum vitamin E level in children with AD.

• Journal of Animal Environmental Science
• /
• v.18 no.sup
• /
• pp.47-54
• /
• 2012

Effect of commercial Direct Fed Microbials (DFM) or protease treated feed (PTF) supplementation on growth rate and biogenic substances such as BUN, glucose, IgG, GOT, GPT and Vitamin A, C, E from Holstein steers was studied for 7 months. Thirty two steers aged 2~3 months were separated with 4 groups for control, DFM (PS), protease (ES) and their mix (PS + ES) supplementally fed 0, 100, 100 and 50 + 50 g/day respectively. Weight gain was averagely higher in PS than any others, although there were no differences significantly. All treatments enhanced to 3~8% of control in dry matter, crude protein and total digestible nutrient (P>0.05). Metabolic diseases with veterinary cure had not shown in this study. Plasma GOT and GPT were lower in the PS and ES than control. Plasma glucose concentration was also lower in PS than the others. Total cholesterol of ES was higher than the others but that of PS is the lowest. Plasma vitamin C was higher in PS than the others. It was shown that dietary PS affected change from glucose to vitamin C with not overloading liver. Conclusionally, PS and ES were shown to enhance metabolic health of steers during growing period.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2013.05a
• /
• pp.91-92
• /
• 2013

• Proceedings of the Korean Institute of Intelligent Systems Conference
• /
• 2007.11a
• /
• pp.71-74
• /
• 2007

오늘날 자연재해 발생 빈도수가 증가하고 있어 ESP(Energy Service Provider)와 대수용가에 서는 예기치 못한 정전 및 에너지 중단과 같은 이벤트가 발생하면 재산상의 손실과 같은 피해가 커지고 있다. 이에 에너지 포털 시스템에서 발생활 수 있는 다양한 이벤트들을 실시간으로 처리할 수 있는 효율적인 이벤트 모델링 개발이 필요하다. 그러나 현재 이러한 이벤트 처리를 위한 이벤트 모델링 개발은 거의 이루어지고 있지 않다. 이에 본 논문에서는 에너지 포털 시스템에서 발생하는 정전과 같은 이벤트와 원격 검침 둥과 같은 부가 서비스를 제공함에 따라 발생하는 이벤트들을 지능을 가진 실시간 에이전트를 사용하여 웹 환경에서 W3C 이벤트 처리 권고안인 DOM 모델을 기반으로 esXML(energy system eXtensible Markup Language)을 사용하여 효율적으로 처리할 수 있는 모델링을 개발하였다.

• Proceedings of the Korean Information Science Society Conference
• /
• 2005.11a
• /
• pp.673-675
• /
• 2005

본 논문에서는 J2ME상에서 JSR-184를 이용한 모바일 3D 엔진을 설계 및 구현하였다. 기존에는 모바일 표준 3D 그래픽 API(C언어 기반)인 OpenGL-ES를 사용하여 모바일 3D 엔진을 제작해, 핸드폰에 애플리케이션을 작동시켰으나, 저수준(Low-Level)의 다양한 기능만 제공함으로써, 다양한 콘텐츠제작 및 호환성에 제약이 많았다. 이에 OpenGL-ES보다 더욱더 다양한 고수준(High-Level)의 API를 제공하면서도 GSM 폰을 중심으로 J2ME상에서 자바환경에 최적화된 모바일표준 3D 표준 API(Java언어 기반)인 JSR-184로 모바일 3D 엔진을 제작하고, 스킨드 메시(Skinned Mesh) 형태를 가지는 모델의 처리속도를 향상시키는 방법을 제시한다.

• Microbiology and Biotechnology Letters
• /
• v.25 no.1
• /
• pp.51-55
• /
• 1997

To utilize the alkaline protease produced by Halomonas sp. ES-10 as an enzyme detergent, the crude enzyme was obtained by methanol precipitation and lyophilization. And it was processed to coated enzyme.The best mixing ratio of components such as coated enzyme, builders, actives, fillers and adjuvants on detergency was examined, and temperature and pH influencing detergency were also tested. Detergency test 0.15% detergent solution was carried out on EMPA test cloth #116 with shaking(90 rpm) for 10 min after 30 min of pretreatment. The detergent which contained coated-enzyme 1%, Zeolite 4A 20%, Tween 80 1. 5%, sodium borate 30%, sodium meta silicate 7.5% and water 40% showed about 90% of washing efficiency at 40$\circ $C and pH 10.0.

• Proceedings of the KIEE Conference
• /
• 1995.11a
• /
• pp.9-11
• /
• 1995

In this paper, tile squirrel case induction motors required multi-objective function are designed. As the objective function of the optimization program, we select the linear combination of loss and mass of motors by using weighting factors. Optimization process is performed by using the evolution strategy (ES). ES is the algorithm that can find the global minimum. To verify validity of the proposed method, a sample design is tried.