• 대한치과보철학회지
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• 제35권2호
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• pp.308-329
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• 1997

A lot of the research paper was reported about the result of influence of IMC (Intra-mobile connector) in the IMZ implant placed solely in the alveolar bone, but reports about the effect of IMC on functional load at state of connecting with natural teeth ere rare. The major propose of this study was find the mechanical character of IMC itself by using the finite element analysis program after simulated variance of condition connected with the natural teeth and implant on funcional load. When first and second premolar was lost, IMZ implant was placed with a diameter of 3.3mm and a length of 13mm with IMC in second premolar area. Rigid connection was done and the non-figid connention was located on the female part of the canine abutment and the implant respectively and then both the infraocclusion of $30{\mu}m$ and the non-infraocclusion under the load of 40kg applied to the portion of the natural teeth, the pontic and the implant. The displacement and the stress of it was estimated and analyzed IMC itself of the rigid connection and the non-rigid connection was grouped. The following result were obtained. 1. In all groups, the displacement of Y-axis was greater than that of X-axis and the aspect of displacement showed that IMC was displaced downward and to the center. 2. There was no differences in the displacement of IMC regardless of the connection type. 3. In the displacement of IMC, I 4 was the least, I 1 and I 3 are similar and I 2 was the greatest. 4. There was no differences in the peak value of maximal stress of IMC regardless of the connection type. 5. In the peak value of maximal stress of IMC, I 4 was the least, I 1 and I 3 were similar, and I 2 was the largest.

• Journal of Microbiology and Biotechnology
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• 제20권11호
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• pp.1563-1570
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• 2010

To construct a highly sensitive detection system for endocrine disruptors (EDs), we have compared the activity of promoters with the n-alkane-inducible cytochrome P450 gene (ALK1), isocitrate lyase gene (ICL1), ribosomal protein S7 gene (RPS7), and the translation elongation factor-1${\alpha}$ gene (TEF1) for the heterologous gene in Yarrowia lipolytica. The promoters were introduced into the upstream of the lacZ or hERa reporter genes, respectively, and the activity was evaluated by ${\beta}$-galactosidase assay for lacZ and Western blot analysis for hER${\alpha}$. The expression analysis revealed that the ALK1 and ICL1 promoters were induced by n-decane and by EtOH, respectively. The constitutive promoter of RPS7 and TEF1 showed mostly a high level of expression in the presence of glucose and glycerol, respectively. In particular, the TEF1 promoter showed the highest ${\beta}$-galactosidase activity and a significant signal by Western blotting with the anti-estrogen receptor, compared with the other promoters. Moreover, the detection system was constructed with promoters linked to the upstream of the expression vector for the hER${\alpha}$ gene transformed into the Y. lipolytica with a chromosome-integrated lacZ reporter gene under the control of estrogen response elements (EREs). It was indicated that a combination of pTEF1p-hER${\alpha}$ and CXAU1-2XERE was the most effective system for the $E_2$-dependent induction of the ${\beta}$-galactosidase activity. This system showed the highest ${\beta}$-galactosidase activity at $10^{-6}\;M\;E_2$, and the activity could be detected at even the concentration of $10^{-10}\;M\;E_2$. As a result, we have constructed a strongly sensitive detection system with Y. lipolitica to evaluate recognized/suspected ED chemicals, such as natural/synthetic hormones, pesticides, and commercial chemicals. The results demonstrate the utility, sensitivity, and reproducibility of the system for identifying and characterizing environmental estrogens.

• 한국치위생학회지
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• 제10권5호
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• pp.831-839
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• 2010

Objectives : The purpose of this study was to examine dental hygiene students' image of dental hygienist and factors affecting the image of dental hygienist in an effort to provide some information useful for dental hygiene education. It's basically meant to recreate the image of dental hygienist as a professional. Methods : The subjects in this study were 310 sophomores and seniors who ere selected by convenience sampling from three different three-year-course colleges located in Gyeonggi Province, Chungcheong Province and Gwangju. After a survey was conducted, the answer sheets from 277 respondents were analyzed except for 33 incomplete ones. The collected data were analyzed with a SPSSWIN 17.0 program, and statistical data on frequency and percentage were obtained to find out the general characteristics of the subjects, and another statistical data on mean and standard deviation for each item were acquired to check their image of dental hygienist. Besides, t-test, ANOVA and Kruscal-Wallis test, one of nonparametric test, were utilized to look for connections between their general characteristics and image of dental hygienist. Results : 1. They gave a mean of 3.53 to the image of dental hygienist, which showed that the dental hygiene students took a favorable view of dental hygienists in general. 2. Their response to four items showed that they looked at the image of dental hygienist in a positive manner. The four items respectively described dental hygienists as professionals, as ones who played a crucial role in the improvement of national oral health, as ones with professional knowledge and sophisticated knowhow, and as neat and decent people. And their response to the following five items indicated that they looked at the image of dental hygienists in a negative way. One was that dental hygienists were considered to be dental nurses, and another was that the occupation of dental hygienist was a demanding and stressful job. The third was that there was no single, fixed title to call dental hygienists, and the fourth was that dental hygiene wasn't recognized as an independent area. The fifth was that the social standing of dental hygienists was sort of high. 3. In regard to the impact of the general characteristics of the dental hygiene students on their image of dental hygienist, they had a more favorable image of dental hygienist when they perceived dental hygiene in a more positive manner(p<0.001). Conclusions : In order to boost the image of dental hygienist, the working conditions of dental hygienists should be improved, and educational programs should be prepared to provided them extended continuing educational opportunities. And the kind of educational environments that could nurture dental hygienists who are convinced and have a vision should be created.

• 한국가정과교육학회지
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• 제10권2호
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• pp.131-144
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• 1998

The lack of characteristical understanding of the Home Economics Education will have difficulty in achieving the goal of the Home Economics Education. To allow the Home Economics Education class in school contribute toward the enhancement of the quality of life, it is inevitable to reinforce the recognition of the importance and necessity of the Home Economics Education and to newly propose a directional guidance forward the coming 21st century. The purpose of this study was to provide basic data for planners of the future Home Economics Education by surveying research administrators in charge of programming the overall school curriculum. This study was conducted under the following procedure to have the outcome as follows ; 1. Answers from respondents of age over 50 or careers over 16 years tended to grasp the goal of Home Economics Education in relation to the enhancement of quality of life rather than to the accumulation of knowledge, skill or capacity. 2. Of all the surveyed, 9.64% regarded Home Economics Education as an essential part of school education and the positive ratio was higher with younger respondents. 3. To the question asking if the high school Home Economics Education could have influence on one's capacity, 97.2% of the answerers said yes. The respondents with career under 15 year showed 100% of positive agreement in this matter and of age under 40 marked much higher ratio of 98.84% than the elders. 4. The overall recognition of the necessity for separate sections in Home Economics Education programmed in accordance with the 6th Revision of High School Curriculum displayed a considerably high ratio, with a little differences among age and job groups. The purpose of this study is to analyze and understand adolescents'culture, thereby to provide fundamental information for Preventive Education for Adolescents'Problems in Home Economic Education. 745 articles collected in computer communication service were used for the analysis. The data were analyzed by frequency and percentage using the SPSS/PC+computer program. The most important results of this study were as follows ; 1) Concerning 'Adolescent's Problems's, problems such as violence, homicide and robbery were treated with the greatest frequency. It was also analyzed that the older generations are highly inclined to understand adolescents'culture as a negative one, for example, 'delinquency'or 'immaturity'. 2) Although the area of 'Adolescents'Education'was treated most frequently reflecting the high educational concern in our society, the significant part of those articles were on wrongful educational administration. As much as 70.6% of the articles analyzed were on 'Education'and 'Problems'. Thereby it may be concluded that the other areas such as health, activities, worries, values and harmful environments ere treated respectively as only a 'subculture'or'fragmentary culture'. The articles containing correlations and interactions among them and certain alternative proposals were very rare. 3) The areas such as 'Worries and Counselling'and 'Health'were occupying only a small portion of the articles. The articles which were treating the practical worries and their resolutions were even rarer. 4) 'Values'of the adolescents tended to be viewed by the older generations very negatively. It was also found that the older generations gave the tendency to see the 'consumption culture'of the adolescents as a kind of 'counter culture'or'subculture'.

• 한국식품영양학회지
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• 제10권1호
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• pp.25-30
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• 1997

Purge와 trap headspace sampler를 사용하여 녹차의 휘발성 성분을 추출, 확인한 후 성분조성을 동시증류추출장치로 추출된 성분가 비교하였다. Headspace법으로는 88개 성분을 확인하였으며 methyl butanal(3.1%), 1-penten-3-ol(5.48%), 2-penten-1-ol(2.89%), hexanal(5.77%), heptanal(2.24%) 등의 비교적 비점이 낮은 성분들과 2,4-heptadienal(4.28%), linalool(2.27%), nonanal(2.06%), 2,6-dimethyl cyclohexanol(2.57%) 및 $\alpha$-pinene(1.52%), caryophyllene(1.70%) 등의 hydrocarbon 화합물, $\beta$-cyclocitral(2.0%), $\alpha$-ionone(2.62%), $\beta$-ionone(2.98%) 같은 carbonyl 화합물이 주요 휘발성 성분으로 확인되었다. SDE법으로는 64개의 성분을 확인하였으며 저비점 성분들보다는 benzyl alcohol(3.79%), linalool(9.52%), terpineol(2.16%), geraniol(2.75%), nerolidol(6.50%) 등의 alcohol 성분들과 $\alpha$-ionone(1.77%), $\beta$-ionone(4.80%), geranyl acetone(1.82%) 등의 ketone 화합물, hexanoic acid(1.45%), nonanoic acid(1.11%) 등의 acid 화합물이 주요 성분으로 확인되었다.

• Journal of Periodontal and Implant Science
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• 제31권4호
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• pp.787-801
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• 2001

The molecular mechanisms control the function of PDL(periodonta1 ligament) cells and/or fibroblasts remain unclear. PDLsl7, PDL-specific gene, had previousely　identified the cDNA for a novel protein from cultured　PDL fibroblasts using subtraction hybridization between gingival fibroblasts and　PDL fibroblasts. The purpose　of this study was to determine the regulation by growth factors and cytokines on　PDLsl7 gene expression in　cultured human periodontal ligament cells and observe the immunohistochemical　localization of PDLsl7 protein in various tissues of mouse. Primary PDL fibroblasts isolated by scraping the root of the extracted human mandibular third molars. The cells were incubated with various concentration of human recombinant $IL-1{\beta}$, PDGF-BB and TGF\;${\beta}$ for 48h nd 2 weeks. At each time point total RNA was extracted and the levels of transcription ere assessed by reverse transcription-polymerase chain reaction (RT-PCR assay). polyclonal antiserum raised against PDLsl7 peptides, CLSVSYNRSYQINE and SEAVHETDLHDGC, were made, and stained the tooth, periodontium, developing bone, bone marrow and mid-palatal suture of the mouse. The results were as follows. 1. PDLsl7 mRNA levels were increased in response to PDGF (10ng/ml) and $TGF\;{\beta}$(20ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF{\beta}$for 48 h. 2. PDLsl7 was up-regulated only by $TGF{\beta}$(20 ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF\;{\beta}$ for 2 weeks and unchanged by the other stimulants. 3. PDLsl7 was a novel protein coding the 142 amino acid peptides in the ORF and the nucleotide sequences of the obtained cDNA from RT-PCR was exactly same as the nucleotides of the database. 4. Immunohistochemical analysis showed that PDLsl7 is preferentially expressed in the PDL, differentiating osteoblast-like cells and stromal cells of the bone marrow in the adult mouse. 5. The expression of PDLsl7 protein was barely detectable in gingival fibroblasts, hematopoetic cells of the bone marrow and mature osteocytes of the alveolar bone. These results suggest that PDLsl7 might upregulated by PDGF-BB or $TGF{\beta}$ and acts at the initial stage of differentiation when the undifferentiated mesenchymal cells in the bone marrow and PDL differentiate into multiple cell types. However, more research needs to be performed to gain a better understanding of the exact function of PDLsl7 during the differentiation of bone marrow mesenchymal and PDL cells.

• 대한화장품학회지
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• 제40권3호
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• pp.279-287
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• 2014

폐경 이후 여성에서 발생되는 에스트로겐의 감소는 피부노화와 밀접한 관련이 있으며, 피부의 정상적 상태와 기능을 저하시키게 된다. 지난 10여 년간 보고된 많은 연구결과를 살펴보면, 에스트로겐은 폐경 이후 여성의 피부에서 콜라겐 감소를 막아주고, 탄력을 회복시키며, 건조한 피부를 개선시키는 등의 피부 안티에이징 효능을 가지는 것을 알 수 있다. 에스트로겐과 유사한 구조로 인해 파이토에스트로겐이라 알려진 이소플라본은 자외선에 의해 유도된 피부 손상을 보호하는 기작이 널리 알려져 왔으나 피부세포에서 에스트로겐과 유사한 안티에이징 효능을 가지는지에 대해서는 많은 연구가 진행되지 않았다. 이에 본 연구에서는 콩류 등에 많이 함유된 이소플라본인 다이드제인이 에스트로겐과 유사한 활성 및 효능을 가지는지 밝히고자 하였다. 먼저 에스트로겐 수용체와의 결합을 통한 transcriptional activity에 미치는 효과를 luciferase assay를 통해 살펴본 결과, 다이드제인은 대조군에 비해 농도 의존적으로 estrogen receptor-dependent transcriptional activity를 유의하게 증가시켰다. 다음으로 사람의 피부 섬유아세포를 이용하여 다이드제인이 세포외 기질단백질 성분들의 발현에 미치는 효과를 조사한 결과, 다이드제인은 콜라겐 타입 I, 콜라겐 타입 IV, 엘라스틴 및 피브릴린-1의 발현을 유의하게 증가시키는 것을 확인할 수 있었다. 모든 실험조건에서 에스트로겐 단독 효능과의 비교 분석을 통해 다이드제인은 에스트로겐과 유사한 효능을 가진다는 것을 확인할 수 있었다. 본 연구 결과를 통해 다이드제인은 기존에 알려진 이소플라본의 광보호 효능과 더불어 파이토에스트로겐 효능을 가짐으로써 갱년기 여성의 피부 안티에이징을 위해 활용할 수 있을 것이라 제안한다.

• 대한치과교정학회지
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• 제20권3호
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• pp.447-462
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• 1990

The objective of this experiment is to observe structural differences in the othodontic movement of vital (control group) and devitalized (experimental group) teeth in the mongrel dogs. The 5 utilized dogs in this experiment were approximately 1 year of age and their average weight was about 12 Kg. Endodontic therapy was performed on the 2nd premolars in upper & lower jaws of each animal under the general anesthesia by intravascular injection of 25mg/kg of pentobarbital sodium. The canals of the teeth were obturated by using gutta percha in conjunction with root canal sealer (AH26 Densply). One of the roots in the 2nd premolars was hemisected to make an extraction space for the devitalized teeth to be moved. The edgewise technique was employed for the movement of the teeth. Orthodontic models and intraoral roentgenograms were taken before and after orthodontic tooth movement. The open coil springs (.010 x .040) were used at interbraket space in order to provide equal forces (75gm) between the teeth in each arch wire. After 13 weeks of active orthodontic tooth movement, dogs were sacrified and the experimental results w ere examined through the intraoral radiography, microscopic examination and scanning electromicroscopic examination at the root sulfate. From the results of the study, the following conclusions may be drawn: The root resolution and cemental deposition were observed within the pressure and tension site in both group. 2. The root resorbed lacunae were observed in the cementum and/or into the dentin in both group. 3. The prominent osteoblastic activities were observed on the alveolar margin in the tension site in both group. 4. A few of blood vessels were observed in the pressure site, but also lots of blood vessels were observed in the tension site especially in the periphery of the alveolar bone in both group. 5. In the pressure site, resorbed lacunae were formed with deep and narrow cavity in the control group; the shallow and wide cavity in the experimental group. 6. In the pressure site, the repaired cementum or cementoid tissue was lined on cementum in the experimental group, but not in the control group. 7. There was no significant difference between external root resolution of endodontically and vital teeth when both were subjected to orthodontic forces.

• 대한가정학회지
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• 제34권4호
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• pp.249-263
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• 1996

According to the design method for constructing the G gori(the traditional Korean jacket)collar, there are differences in form after its completion. In the construction design of the midsection of the gusset as in that of the basic G gori, not only was the positioning of the two sides of the collar not smooth, but also, when the collar strip was attached, there would be an imbalance on one side or the other so that it was difficult to achieve symetry. Therefore in this project, by applying the pattern design of the gusset midsection to that of the collar midsection, not only did attaching the collar and the collar strip create a visual effect by equalizing the lengths of the tow sides, but the construction was also easy. This method of construction introduces the three-dimensional aspect of the human form in its conic, spherical and cylindrical aspects, so that, through schematizing the scientific character of Korea's clothing, its appropriateness is verified. As a matter of fact, since th-ere in no standardization of pattern whether for educational use or for mass-produced clothing, so that even the patterns used in computer-assisted design are executed according to the designer's personal skill, the reality is that after completion of construction the quality has not been uniform. For this schematization, inverse calculation of measurements pertaining to the calculation formula and of teaching materials has been referenced. In particular, the partial requlation of the calculation formula pertaining to the basic pattern construction, the method of making the collar midsection, and the construction method of the extreme and mean ratio adjustment can be adjusted for all measurements, thereby providing the establishment of a design criterion and the possibility of the standardization of construction methods. The production method for the pattern design is as follows: 1) The conic angle for the G gori's girth, length and neck width is fixed at 70 degrees. 2) The radius of the cone is B/2. 3) The calculation formula is B/4 + 1.5cm 4) The armhole formula is B/4, the same as in the basic method. 5) The width formula is B/10. 6) The ratio of the collar junction(width of collar + width of collar strip)to the gusset length is 5:8. 7) The length of the side seam is a length intersecting the armhole line and the conic radius (B/2), that is an arc that exceeds the length of the G gori's midsection; the component ratio of this length to the collar junction is accordingly 13:5. 8) The curve frame length of the back midsection is an arc exceeding B/4(the armhole line). 9) The ratio of the sleeve opening calculation formula to the armhole length is 8:5, forming an arc with the midsection length.

• 치위생과학회지
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• 제10권5호
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• pp.315-321
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• 2010

본 연구는 2009년 8월부터 2009년 11월까지 편의추출된 서울, 인천, 대구지역 치과 병의원 3곳에서 1개 이상 3개 이하 치아에 대해 임플란트 시술을 받은 20세 이상의 남녀 성인 환자 중 102명을 대상으로 하여 3개 이하의 임플란트 고정성 보철물 장착 전후의 구강건강관련 삶의 질(OHIP-14) 변화에 관하여 조사하고 다음과 같은 결론을 얻었다. 1. 3개 이하의 임플란트 고정성 보철물 장착 후 OHIP-14 총점은 치료 전에 비해 유의하게 감소하여(p<0.001) 구강건강관련 삶의 질이 향상된 것으로 나타났다. 2. 인구통계적 특성에 따른 보철물 장착 전 시점의 OHIP-14 총점은 성별(p=0.01), 경제적 수준별(p=0.04), 교육수준별(p=0.01)에는 유의한 차이가 있었으나 보철물 장착 후에는 OHIP-14 총점의 유의한 차이가 나타나지 않았다. 3. 3개 이하의 임플란트 고정성 보철물 장착 후 North Texas Periodontal Associates에서 제시한 모형의 저작 기능, 사회적 기능, 심리적 기능 만족도에 있어서도 유의하게 변화하였으나(p<0.001) 세부지표 중 구강건강에 대한 염려의 전후 변화는 유의하지 않았다. 4. 3개 이하의 임플란트 고정성 보철물 장착 후 구강건강관련 관심도 변화에 있어서 잇솔질 횟수 증가(p<0.001), 주기적 구강검진(p<0.001), 구강검진필요 인지(p<0.001), 구강질병치료 인지(p<0.001) 등에 있어서도 유의하게 변화하였다. 따라서, 본 연구에서는 소수 치아 결손 시 임플란트를 이용한 고정성 보철물 장착이 구강건강관련 삶의 질, 저작기능, 사회적 기능, 심리적 기능 만족도, 구강건강관련 관심도를 향상시킬 수 있다고 판단되었다.