Browse > Article
http://dx.doi.org/10.15230/SCSK.2014.40.3.279

The Phytoestrogenic Effect of Daidzein in Human Dermal Fibroblasts  

Kim, Mi-Sun (R&D Center, LG Household & Healthcare Ltd.)
Hong, Chan Young (R&D Center, LG Household & Healthcare Ltd.)
Lee, Sang Hwa (R&D Center, LG Household & Healthcare Ltd.)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.3, 2014 , pp. 279-287 More about this Journal
Abstract
Estrogen deficiency results in a reduction of skin quality and function in postmenopausal women. Over the past decade, many studies have supported that estrogen provides anti-aging effects as a result of the ability of estrogen to prevent skin collagen decline, restore skin elasticity, and increase skin hydration in postmenopausal women skin. Due to their structural similarity with estrogen, isoflavones have been called phytoestrogens. Photoprotective effects of isoflavones are well established while their estrogenic-like activities are not fully understood in human skin. In this study, we investigated whether daidzein, an effective isoflavone, has phytoestrogenic activity and induces transcriptional change of extracellular matrix components in dermal fibroblasts. We examined the luciferase activity of daidzein and ${\beta}$-estradiol using transiently transfected NIH3T3-ERE cells. The estrogenic receptor-dependent transcriptional activity was increased in a dose-dependent manner when treated with daidzein, with a maximum of 2.5-fold induction at $10{\mu}g/mL$ of daidzein compared with non-treated control. In addition, daidzein significantly in creased the expressions of collagen type I, collagen type IV, elastin, and fibrillin-1 in human dermal fibroblasts. By comparing with the effects of ${\beta}$-estradiol through out all the experiments, we confirmed that daidzein had estrogenic activity and function in fibroblasts. These results suggest that daidzein-based application, having both photoprotective and phytoestrogenic effects, may be a powerful approach for skin anti-aging of postmenopausal women.
Keywords
daidzein; estrogenic activity; extracellular matrix; postmenopausal women;
Citations & Related Records
연도 인용수 순위
  • Reference
1 E. D. Son, J. Y. Lee, S. Lee, M. S. Kim, B. G. Lee, I. S. Chang, and J. H. Chung, Topical application of 17-$\beta$-estradiol increases extracellular matrix protein synthesis by stimulating TGF-$\beta$ signaling in aged human skin in vivo, J. Invest. Dermatol., 124, 1149 (2005).   DOI   ScienceOn
2 C. Kainz, G. Gitsch, J. Stani, G. Breitenecker, M. Binder, and J. B. Schmidt, When applied to facial skin, does estrogen ointment have systemic effects? Arch. Gynecol. Obstet., 253, 71 (1993).   DOI
3 J. Y. Lin, J. A. Tournas, J. A. Burch, N. A. Monteiro-Riviere, and J. Zielinski, Topical isoflavones provide effective photoprotection to skin, Photodermatol. Photoimmunol. Photomed., 24, 61 (2008).   DOI   ScienceOn
4 B. Iovine, M. L. Iannella, F. Gasparri, V. Giannini, G. Monfrecola, and M. A. Bevilacqua, A comparative analysis of the photo-protective effects of soy isoflavones in their aglycone and glucoside forms, Int. J. Mol. Sci., 13, 16444 (2012).   DOI
5 M. Masilamani, J. Wei, and H. A. Sampson, Regulation of the immune response by soybean isoflavones, Immunol. Res., 54, 95 (2012).   DOI   ScienceOn
6 R. L. Jackson, J. S. Greiwe, and R. J. Schwen, Ageing skin: oestrogen receptor $\beta$ agonists offer an approach to change the outcome, Exp. Dermatol., 20, 879 (2011).   DOI
7 G. G. Kuiper, J. G. Lemmen, B. Carlsson, J. C. Corton, S. H. Safe, P. T. van der Saag, B. van der Burg, and J. A. Gustafsson, Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor $\beta$, Endocrinology, 139, 4252 (1998).   DOI
8 S. Ogawa, S. Inoue, T. Watanabe, H. Hiroi, A. Orimo, T. Hosoi, Y. Ouchi, and M. Muramatsu, The complete primary structure of human estrogen receptor $\beta$ (hER $\beta$) and its heterodimerization with ER $\alpha$ in vivo and in vitro, Biochem. Biophys. Res. Commun., 243, 122 (1998).   DOI
9 M. T. Patriarca, A. R. Barbosa de Moraes, H. B. Nader, V. Petri, J. R. Martins, R. C. Gomes, and J. M. Soares Jr, Hyaluronic acid concentration in postmenopausal facial skin after topical estradiol and genistein treatment: a double-blind, randomized clinical trial of efficacy, Menopause, 20, 336 (2013).
10 A. B. Moraes, M. A. Haidar, J. M. Soares Junior, M. J. Simoes, E. C. Baracat, and M. T. Patriarca, The effects of topical isoflavones on postmenopausal skin: double-blind and randomized clinical trial of efficacy, Eur. J. Obstet. Gynecol. Reprod. Biol., 146, 188 (2009).   DOI   ScienceOn
11 P. Walter, S. Green, G. Greene, A. Krust, J. M. Bornert, J. M. Jeltsch, A. Staub, E. Jensen, G. Scrace, M. Waterfield, and P. Chambon, Cloning of the human estrogen receptor cDNA, Proc. Natl. Acad. Sci. USA, 82, 7889 (1985).   DOI
12 G. Pelletier and L. Ren, Localization of sex steroid receptors in human skin, Histol. Histopathol., 19, 629 (2004).
13 M. Saceda, M. E. Lippman, P. Chambon, R. K. Lindsey, M. Puente, and M. B. Martin, Regulation of the estrogen receptor in MCF-7 cells by estradiol, Mol. Endo., 2, 1157 (1988).   DOI   ScienceOn
14 S. J. Ellison-Zelski, N. M. Solodin, and E. T. Alarid, Repression of ESR1 through actions of estrogen receptor $\alpha$ and Sin3A at the proximal promoter, Mol. Cell. Biol., 29, 4949 (2009).   DOI
15 M. P. Brincat, Y. M. Baron, and R. Galea, Estrogens and the skin, Climacteric, 8, 110 (2005).   DOI   ScienceOn
16 M. Brincat, S. Kabalan, J. W. Studd, C. F. Moniz, J. de Trafford, and J. Montgomery, A study of the decrease of skin collagen content, skin thickness, and bone mass in the postmenopausal woman, Obstet. Gynecol., 70, 840 (1987).
17 L. Rittie, S. Kang, J. J. Voorhees, and G. J. Fisher, Induction of collagen by estradiol: difference between sun-protected and photodamaged human skin in vivo, Arch. Dermatol., 144, 1129 (2008).
18 E. C. Naylor, R. E. Watson, and M. J. Sherratt, Molecular aspects of skin ageing, Maturitas, 69, 249 (2011).   DOI   ScienceOn
19 F. Vazquez, S. Palacios, N. Aleman, and F. Guerrero, Changes of the basement membrane and type IV collagen in human skin during aging, Maturitas, 25, 209 (1996).   DOI   ScienceOn
20 S. Stevenson and J. Thornton, Effect of estrogens on skin aging and the potential role of SERMs, Clin. Interv. Aging, 2, 283 (2007).   DOI
21 I. M. Braverman, Elastic fiber and microvascular abnormalities in aging skin, Clin. Geriatr. Med., 5, 69 (1989).
22 J. L. Bolognia, I. M. Braverman, M. E. Rousseau, and P. M. Sarrel, Skin changes in menopause, Maturitas, 11, 295 (1989).   DOI   ScienceOn
23 R. Punnonen, P. Vaajalahti, and K. Teisala, Local oestriol treatment improves the structure of elastic fibers in the skin of postmenopausal women, Ann. Chir. Gynaecol. Suppl., 202, 39 (1987).
24 A. H. Lichtenstein, Soy protein, isoflavones and cardiovascular disease risk, J. Nutr., 128, 1589 (1998).
25 D. F. Archer, Postmenopausal skin and estrogen, Gynecol. Endocrinol., 28, 2 (2012).
26 C. C. Zouboulis and E. Makrantonaki, Hormonal therapy of intrinsic aging, Rejuvenation. Res., 15, 302 (2012).   DOI   ScienceOn
27 N. Nisar and N. A. Sohoo, Frequency of menopausal symptoms and their impact on the quality of life of women: a hospital based survey, J. Pak. Med. Assoc., 59, 752 (2009).
28 J. Calleja-Agius, M. Brincat, and M. Borg, Skin connective tissue and ageing, Best Pract. Res. Clin. Obstet. Gynaecol., 27, 727 (2013).   DOI
29 Y. Y. Shu and H. I. Maibach, Estrogen and skin: therapeutic options, Am. J. Clin. Dermatol., 12, 297 (2011).   DOI   ScienceOn
30 J. B. M. Schmidt, M. M. Binder, G. M. Demschik, C. M. Bieglmayer, and A. M. Reiner, Treatment of skin aging with topical estrogens, Int. J. Pharm. Compd., 2, 270 (1998).
31 L. B. Dunn, M. Damesyn, A. A. Moore, D. B. Reuben, and G. A. Greendale, Does estrogen prevent skin aging? Results from the first national health and nutrition examination survey (NHANES I), Arch. Dermatol., 133, 339 (1997).   DOI
32 P. G. Sator, M. O. Sator, J. B. Schmidt, H. Nahavandi, S. Radakovic, J. C. Huber, and H. Honigsmann, A prospective, randomized, double-blind, placebo-controlled study on the influence of a hormone replacement therapy on skin aging in postmenopausal women, Climacteric, 10, 320 (2007).   DOI   ScienceOn
33 K. C. Chang, Y. Wang, I. G. Oh, S. Jenkins, L. P. Freedman, C. C. Thompson, J. H. Chung, and S. Nagpal, Estrogen receptor $\beta$ is a novel therapeutic target for photoaging, Mol. Pharmacol., 77, 744 (2010).   DOI   ScienceOn
34 E. Kohl, J. Steinbauer, M. Landthaler, and R. M. Szeimies, Skin ageing, J. Eur. Acad. Dermatol. Venereol., 25, 873 (2011).   DOI   ScienceOn