• 생명과학회지
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• 제27권1호
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• pp.95-99
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• 2017

우리는 이전실험에서 배양세포를 사용하여 4종류의 천연물(Corydaline, (${\pm}$)-Car-3-ene-2,5-dione, Cinobufagin, Corilagin)이 ERAP1와 FOXO1 (DFA16) 유전자발현을 2배 이상 상승시키는 것을 증명하였다. 본 실험은 1% agar, 5% sucrose, natural compound $20{\mu}l$를 넣은 먹이를 만들어 4시간 starvation후에 4시간 동안 먹였다. Cinobufagin와 Corilagin를 먹이면 대조군에 비하여 6-8일 정도 더 생존하였다. RT-PCR 실험결과 ERAP1와 FOXO1 유전자 발현을 조절하는 것이 증명되었다. 산업곤충질병 진단과 치료에 사용될 것이며, 초파리를 대신한 생쥐에서도 동일한 결과를 실험하여 수명연장을 위한 신약으로 발전시킬 것이다.

• 생명과학회지
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• 제26권3호
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• pp.359-363
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• 2016

한약진흥재단(NIKOM)의 640-천연물 라이브러리를 PC12세포에 10 μg/ml/day로 처리하였다. 면역강화에 관여하는 ERAP1 (Endoplasmic Reticulum AminoPeptidase 1)과 수명연장에 관여하는 FOXO1 (FOXO-family transcription factor) 유전자발현을 RT-PCR로 확인하였다. ERAP1 유전자를 1.5-2배까지 상승시킨 것은 22개, 2배 이상 발현시킨 것은 14개였다. FOXO1 유전자를 1.5-2배까지 상승시킨 것은 총 24개, 2배 이상 발현시킨 것은 4개였다. 동시에 ERAP1과 FOXO1 유전자를 2배 이상 상승시키는 것은 족도리풀(Asiasari radix)에서 분리된 (±)-Car-3-ene-2,5-dione, 두꺼비(Bufonis venenum)에서 분리된 Cinobufagin, 대국(Euphorbiae pekinensis)에서 분리된 Corilagin, 현호색(Corydalis tuber)에서 분리된 Corydaline 4종이다. 이들 4종의 천연물은 초파리를 이용한 개체의 수명연장실험에 사용될 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제5권3호
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• pp.419-426
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• 1992

After the virtual eradication of cattle during World War II, Papua New Guinea herds were rebuilt with Shorthorn and Aberdeen Angus cattle from Australia. These, and Red Sindhi and Sahiwal, imported in 1952, were considered unsuitable breeds. In 1954, Department of Agriculture, Stock and Fisheries imported three Brahman bulls and three heifers from Texas and in 1960 began importations of Afrikaner from Queensland. In Central Province, Brahmans were crossed with Angus and at Erap (Morobe) the hottest place in Papua New Guinea, Shorthorns were crossed with Afrikaners. In 1965, Brahman and Brahman-cross were sent to Erap. Records of breeding and growth rates were collected for use in upgrading in cattle of the basis of performance, not pedigree. The data are not ideal for genetic analysis, since no control groups were maintained. Birth weights (BWT), weaning weights (WWT) and calving intervals (CI) were analysed for the period 1969-1978. After exclusion of unsatisfactory data, 2,514 calf records were used, including both breeds from 1969 to 1973, but only Brahman-cross subsequently. Breed mean BWT ranged only from 30.6 to 33.8 kg. As Brahman content increased, BWT decreased and WWT increased; within a genotype, there was a negative maternal effect of high Brahman content on BWT and a positive effect on WWT which ranged from 138 to 174 kg. Afrikaner calves had heavier BWT but lighter WWT. As expected, bulls were heaviest, heifers lightest and mature cows bore and reared heavier calves. Calving interval (405 days, equivalent to 90% calving) was unaffected by breed but 4-year old cows averaged 423 days. Breed differences in BWT and WWT are consistent with the body of literature on performance of Brahmans and Afrikaners. Since cattle tick are not present and internal parasites are insignificant at Erap, the superiority of Brahmans indicates that they were better at utilizing the mediocre quality grazing of the Markham Valley or were more heat tolerant. Performance selection over ten years resulted in the virtual elimination of Afrikaners, with the final genotype approximately 9/16 Brahman, 3/8 Shorthorn and 1/16 Afrikaner.