• Nutrition Research and Practice
• /
• v.13 no.1
• /
• pp.17-22
• /
• 2019

BACKGROUND/OBJECTIVES: Successful recovery of an animal from exercise is essential, especially prior to the next exercise session. This study was conducted to find an effective exercise-to-rest period ratio for the restoration of energy sources and replenishment of anti-oxidative status in tissue after exercise. MATERIALS/METHODS: Thirty-two rats were assigned to either non-training or training exercise groups for 5 weeks. After that period, the two groups were subdivided into four smaller groups: non-exercise (NE), exercise 0.5 hour and rest 1 hour (ER0.5:1), exercise 1 hour and rest 1 hour (ER1:1), exercise 2 hours and rest 1 hour (ER2:1). RESULTS: In the training group animals and compared to the NE group, the levels of plasma glucose after the rest period were significantly high in all ER groups but highest in the ER2:1 group. Similarly, the liver glycogen level was highest in the ER2:1 group. The plasma FFA level reached the highest level in the ER2:1 group but was similarly high in the ER0.5:1 group. Liver TG level was unchanged in the ER2:1 and ER1:1 groups but was significantly high in the ER0.5:1 group. Muscle TG levels were decreased in all three ER groups. Plasma protein levels were significantly high in the ER2:1 and ER0.5:1 groups. In both training animal and non-training animals, the liver protein levels did not change significantly between the NE and ER groups, irrespective of the exercise-to-rest ratio. In the training animal group, muscle protein level was significantly low in the ER2:1 and ER0.5:1 groups. The activity levels of superoxide dismutase and catalase, as well as the malondialdehyde concentration, were not significantly different between NE and ER groups, irrespective of the exercise-to-rest period ratio. CONCLUSIONS: These results indicate that animals provided with a 0.5:1 to 1:1 exercise-to-rest period ratio can restore their muscle energy sources and recover their anti-oxidative defense system.

• Tribology and Lubricants
• /
• v.18 no.1
• /
• pp.16-23
• /
• 2002

For increasing the yield stress of ER fluids, the compressing ER electrode was developed and the compressing electrorheological (ER) behavior of anhydrous ER fluids in silicone oil of phosphorous ester cellulose powder was investigated. Under constant electric field, not only the current density but also the yield stress of anhydrous ER fluids were studied as varying the compressing length of ER electrode distance. From the experimental results the compressing of ER electrode had a large influence to the ER properties of anhydrous ER fluids. The current density was proportional to the compressing length of ER electrode under constant electric field and volume fraction also tile compressing yield stress was proportional to the volume fraction of dispersed particles under constant electric field and compressing length. When the ER electrode was compressed with 150mm after charging the electric field, 4 kV, tile yield stress of phosphoric ester cellulose ER fluids increased to thirteen times comparing with the yield stress measured at normal electrode.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2008.04a
• /
• pp.55-74
• /
• 2008

Previously, we have shown that hypoxia, through HIF-1, induces ligand-independent $ER{\alpha}$ activation and the physical interaction of HIF-1 and $ER{\alpha}$. However, the effect of hypoxia on the transactivation of $ER{\beta}$ is not yet known. In the present study, we found that hypoxia activated the $ER{\beta}$-mediated transcriptional response in the HEK 293 cell line, as determined by the transient expression of$ER{\beta}$ and ER-responsive reporter plasmids. The hypoxia-induced estrogen response element-mediated transcriptional response was dependent on $ER{\beta}$ expression and was inhibited by the ER antagonist ICI 182,780. Transactivation of $ER{\beta}$ was induced by the expression of HIF-$1{\alpha}$ under normoxic conditions, as determined by the expression of oxygen-independent stable GFP-HIF-$1{\alpha}$. HIF-$1{\alpha}$-induced $ER{\beta}$ transactivation was abolished by the inhibition of HIF-$1{\alpha}$ activation. This was determined by using chemical inhibitors for the MAPK pathway. In addition, HIF-$1{\alpha}$ interacted with $ER{\beta}$ in a mammalian-two hybrid assay. We conclude that hypoxia activates $ER{\beta}$ in a ligand-independent manner, possibly through the interaction of HIF-$1{\alpha}$ and $ER{\beta}$.

• Molecules and Cells
• /
• v.41 no.8
• /
• pp.705-716
• /
• 2018

The endoplasmic reticulum (ER) is a critical organelle for protein synthesis, folding and modification, and lipid synthesis and calcium storage. Dysregulation of ER functions leads to the accumulation of misfolded- or unfolded-protein in the ER lumen, and this triggers the unfolded protein response (UPR), which restores ER homeostasis. The UPR is characterized by three distinct downstream signaling pathways that promote cell survival or apoptosis depending on the stressor, the intensity and duration of ER stress, and the cell type. Mammalian cells express the UPR transducers IRE1, PERK, and ATF6, which control transcriptional and translational responses to ER stress. Direct links between ER stress and immune responses are also evident, but the mechanisms by which UPR signaling cascades are coordinated with immunity remain unclear. This review discusses recent investigations of the roles of ER stress in immune responses that lead to differentiation, maturation, and cytokine expression in immune cells. Further understanding of how ER stress contributes to the pathogenesis of immune disorders will facilitate the development of novel therapies that target UPR pathways.

• The Korean Journal of Rheology
• /
• v.10 no.3
• /
• pp.165-172
• /
• 1998

본논문에서는 전기장 부하에 따라 유동성질이 변화하는 ER유체의 빙햄특성을 실험 적으로 연구하였다. 특히 ER유체의 빙행특성에 영향을 주는 여러인자중 전극 간격 및 재질 에 따른 ER유체의 항복전단응력과 전류밀도의 변화를 온도에 따라 고찰하였다. 이를 위하 여 전극 간격을 가변시킬수 있는 전기 점도계를 세가지 재질로 자체 제작하였다. 전극간격 은 0.75 mm, 1.00mm 및 1.25 mm 로 설정하였으며 전극 재질은 스테인레스 스텔, 동 그리 고 기계구조용 탄소강(SMS45C)을 사용했다. 한편 실험에 사용된 ER유체는 자체 조성한 수 계 ER유체인 ERF-1과 외국의 우수하다고 알려진 비수계 ER유체인 ERF-2 두가지를 선택 하였다. 실험은 $25^{\circ}C$와 7$0^{\circ}C$ 및 10$0^{\circ}C$에서 수행하였으며 전기장은 0-4kV/mm 범위에서 온 도 및 ER유체의 종류에 따라 부하 가능한 전압까지 공급하였다. 전단변형률 50, 100, 150, 200, 400, 600, 800, 1000 및 1200 s-1에서 얻은 전단응력 실험결과로부터 최소오차선형법을 이용하여 전단변형률 영에서 동적 항복전단응력 값을 도출하였으며 그결과로부터 전극 간격 및 재질에 따른 ER효과의 변화를 고찰하였다. 또한 상온과 10$0^{\circ}C$에서 4kV/mm의 전기장을 부하하여 전기장에대한 ER유체의 응답특성을 실험을 수행했다.

• Journal of Life Science
• /
• v.31 no.9
• /
• pp.796-805
• /
• 2021

Hepatic endoplasmic reticulum (ER) stress contributes to the development of steatosis and insulin resistance. The components of unfolded protein response (UPR) regulate lipid metabolism. Recent studies have reported an association between ER stress and aberrant cellular lipid control; moreover, research has confirmed the involvement of sterol regulatory element-binding proteins (SREBPs)-the central regulators of lipid metabolism-in the process. However, the exact role of SREBPs in controlling lipid metabolism during ER stress and its contribution to fatty liver disease remain unknown. Here, we show that SREBP-1c deficiency protects against ER stress-induced hepatic steatosis in mice by regulating UPR, inflammation, and fatty acid oxidation. SREBP-1c directly regulated inositol-requiring kinase 1α (IRE1α) expression and mediated ER stress-induced tumor necrosis factor-α activation, leading to a reduction in expression of peroxisome proliferator-activated receptor γ coactivator 1-α and subsequent impairment of fatty acid oxidation. However, the genetic ablation of SREBP-1c prevented these events, alleviating hepatic inflammation and steatosis. Although the mechanism by which SREBP-1c deficiency prevents ER stress-induced inflammatory signaling remains to be elucidated, alteration of the IRE1α signal in SREBP-1c-depleted Kupffer cells might be involved in the signaling. Overall, the results suggest that SREBP-1c plays a crucial role in the regulation of UPR and inflammation in ER stress-induced hepatic steatosis.

• Korean Journal of Microbiology
• /
• v.43 no.1
• /
• pp.54-58
• /
• 2007

Fermented soybean, Chungkookjang contains microorganism, enzyme, and diverse bioactive compounds. Isoflavones in Chungkookjang might suppress breast and prostate cancers. Using HPLC and Mass analyses, it was found that 100% ethanol extract of Chungkookjang contains aglycone forms such as genistein, daidzein, and glycitein. 8-OH-genistein, 8-OH-daidzein were not found. There are two estrogen receptors, $ER{\alpha},ER{\beta}$. $ER{\alpha}$ might stimulate proliferation of breast and prostate cancer cells, and $ER{\beta}$ might suppress their growth. Using yeast transactivation assay under the control of human ER expression, it was demonstrated that isoflavones in Chungkookjang can stimulate $ER{\beta}_{1}$, selectively.

• Biomedical Science Letters
• /
• v.12 no.4
• /
• pp.435-438
• /
• 2006

In eukaryotes, ER stress induces UPR (unfolded protein response) via IRE1 activation which sends a molecular signal for XBP1 mRNA splicing in the cytosol. During this mRNA splicing, 23 nt removed in which contains PstI site and then resulting XBP1 product is not digested with PstI restriction enzyme. In this study, using this XBP1 mRNA splicing mechanism, the effect of heat shock on thyrocytes is studied, because heat shock response in the thyrocytes needs more study to understand thyroid physiology under alternative environments. ER inducible drugs (tunicamycin, DTT, $Ca^{2+}$ ionopore A23187, BFA) induce ER stress in the thyrocytes. From 3 hours after heat shock, ER stress is induced and which is reversible when heat shock is without. While $Ca^{2+}$ ionopore A23187 is reversible from ER stress by washing out the drug, thapsigagin is irreversible. Other ER inducible drugs are not so sensitive to ER stress repairing. XBP1 mRNA splicing in a cell is very available method to detect ER stress. It needs only a small quantity of total RNA and processing also very easy.

• Asian Pacific Journal of Cancer Prevention
• /
• v.13 no.4
• /
• pp.1111-1113
• /
• 2012

The ER-/PR+ breast tumor may be the result of a false ER negative result. The aim of this study was to investigate whether there is a difference in patient and tumor characteristics of the ER-/PR+ phenotype in an Asian setting. A total of 2629 breast cancer patients were categorized on the basis of their age, ethnicity, tumor hormonal receptor phenotype, grade and histological type. There were 1230 (46.8%) ER+/PR+, 306 (11.6%) ER+/PR-, 122 (4.6%) ER-/PR+ and 972 (37%) ER-/PR-. ER-/PR+ tumors were 2.5 times more likely to be younger than 50 years at diagnosis (OR: 2.52; 95% CI: 1.72-3.67). Compared to ER+/PR+ tumors, the ER-/PR+ phenotype was twice more likely to be associated with grade 3 tumors (OR:2.02; 95%CI: 1.00-4.10). In contrast, compared to ER-/PR- tumors, the ER-/PR+ phenotype was 90% less likely to be associated with a grade 3 tumor (OR: 0.12; 95%CI:0.05-0.26), and more likely to have invasive lobular than invasive ductal histology (OR: 3.66; 95%CI: 1.47-9.11). These results show that the ER-/PR+ phenotype occurs in a younger age group and is associated with intermediate histopathological characteristics compared to ER+/PR+ and ER-/PR- tumors. This may imply that it is a distinct entity and not a technical artifact.

• Tribology and Lubricants
• /
• v.15 no.1
• /
• pp.8-16
• /
• 1999

The electrorheological (ER) behavior of suspensions in silicone oil of phosphoric ester cellulose powder (average particle size : 18$\pm$1 ${\mu}{\textrm}{m}$) was investigated on the elevated temperature up to 10$0^{\circ}C$. For development of anhydrous ER fluids using at wide temperature range, it should be researched to how the effect of temperature on the ER activities. As a first step, the anhydrous ER suspensions mixing with the phosphoric ester cellulose particles which were made from the phosphoric ester reaction of cellulose were measured. As increasing the temperature, not only the analysis of electrical properties such as dielectric constant current density and electrical conductivity but also the rheological properties of ER fluids were studied. From the experimental results, the temperature had a large influence to the ER properties of anhydrous ER fluids. The current density, conductivity and elecoorheological effect ($\tau$$_{A}$$\tau$$_{0}$) of phosphoric ester cellulose ER fluids were proportional to the temperature with power law. And the shear stress of them was closely related with the square of dielectric constant mismatch parameter ($\beta$$^2$) under constant shear rate and electric field.d.