• Food Science and Biotechnology
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• 제17권3호
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• pp.587-593
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• 2008

Microbial exopolysaccharide (EPS) is a biothickener that can be added to a wide variety of food products, where it serves as a viscosifying, stabilizing, emulsifying, and gelling agent. The objective of this study was to investigate the optimum conditions of pH, incubation temperature, and whey protein concentration (WPC) for EPS production by Lactobacillus rhamnosus ATCC 9595. We found that maximal EPS production was achieved at a pH of 5.5 and temperature of $37^{\circ}C$. At the same fermentation conditions, EPS production was affected by the addition of L. rhamnosus GG (a weak-EPS producer). After growth for 24 hr, total EPS production was $583{\pm}15.4mg/L$ in the single culture system, and $865{\pm}22.6\;mg/L$ in the co-culture system with L. rhamnosus GG. Based on the presence of WPC, EPS production dramatically increased from $583{\pm}15.4$ (under no WPC supplementation) to $1,011{\pm}14.7\;mg/L$ (under supplementation with 1.0% WPC). These results suggest that WPC supplementation and the co-culture systems coupled with small portions of weak-EPS producing strain can play an important role in the enhancement of EPS production.

• 한국지반공학회논문집
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• 제18권4호
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• pp.75-83
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• 2002

본 연구에서는 준설토, EPS 그리고 고화재를 혼합한 경량혼합토의 역학적 특성을 일축 및 삼축압축시험을 통하여 고찰하였다. 역학적 특성은 다양한 준설토의 초기 함수비, EPS 함유율, 시멘트 함유율 그리고 양생압력에 대한 경량혼합토의 압축강도에 대하여 연구하였다. 삼축압축상태에서 EPS를 함유한 경량혼합토의 압축강도는 유효구속압에 의존 하지 않는 것으로 나타났다. EPS 함유율이 감소할수록($A_E$<2%) 그리고 시멘트 함유율이 증가할수록($A_c$>2%) 삼축압축강도-변형 거동특성은 극한 압축강도를 지나 급격한 압축강도의 감소를 보이는 시멘트 혼합토의 압축강도-변형 거동특성과 유사한 것으로 나타났다. 200kpa 이상의 압축강도를 요구하는 개량지반에 본 경량혼합토를 적용하는 경우에서 적절한 준설토의 초기함수비, EPS 함유율은 각각 약 165%~175%이상 그리고 3%~4%이상으로 제시할 수가 있었다. 삼축 및 일축압축상태에서 극한 압축강도는 시멘트 함유율이 2%이상에서 거의 증가하지 않음으로서 한계 시멘트 함유율은 2%로 제시할 수가 있었다.

• 한국포장학회지
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• 제6권1호
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• pp.47-52
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• 2000

개발한 EPS 포장상자가 사과의 신선도 유지효과 기능이 있는지의 여부를 조사코자 사과를 포장하여 $20^{\circ}C$ 저장하면서 골판지상자와 0.02와 0.04mm 두께의 LDPE 필름, 기능성 MA필름과 50배로 발포시킨 EPS상자에 사과를 15kg씩 포장하여 포장재간의 신선도 유지효과를 비교하였다. 저장 40일 후 중량감소는 대조구는 5.7%, EPS 상자는 0.5%의 중량감소가 나타났고, 비타민C도 2.06과 2.31 mg%로 EPS 포장구가 높게 유지되고 있었다. 당도와 산도는 포장구간에 큰 차이가 없었으며 부패의 경우 대조구는 상자당 3-5개과가 발생했으나 EPS포장구는 발생하지 않았으며 외관도 양호한 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제28권5호
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• pp.663-670
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• 2018

The present study focused on the production, characterization, and in vitro prebiotic evaluation of an exopolysaccharides (EPS) from Bacillus sonorensis MJM60135 isolated from ganjang (fermented soy sauce). Strain MJM60135 showed the highest production ($8.4{\pm}0.8g/l$) of EPSs compared with other isolates that were screened for EPS production based on ropy culture morphology. Furthermore, MJM60135 was cultured in 5 L of medium and the EPS was extracted by ethanol precipitation. The emulsification activity of the EPS was higher in toluene than in o-xylene. Fourier transform infrared spectroscopy analysis showed the presence of hydroxyl and carboxyl groups and glycosidic linkages. The isolated EPS contained mannose and glucose, as observed by thin-layer chromatography analysis of the EPS hydrolysate. Lactic acid bacteria (LAB) and pathogenic E. coli K99 and Salmonella enterica serovar Typhimurium were tested for their growth utilizing the EPS from B. sonorensis MJM60135 as the sole carbon source for its possible use as a prebiotic. All the tested LAB exhibited growth in the EPS-supplied medium compared with glucose as carbon source, whereas the pathogenic strains did not grow in the EPS-supplied medium. These findings indicate that the EPS from B. sonorensis MJM60135 has potential application in the bioremediation of hydrocarbons and could also be used as a prebiotic.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권5호
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• pp.405-413
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• 2004

The rheological properties of an exopolysaccharide, EPS-R, produced by the marine bacterium Hahella chejuensis strain 96CJ 10356 were investigated. The $E_{24}$ of $0.5\%$ EPS-R was $89.2\%$, which was higher than that observed in commercial polysaccharides such as xanthan gum ($67.8\%$), gellan gum ($2.01\%$) or sodium alginate ($1.02\%$). Glucose and galactose are the main Sugars in EPS-R, with a molar ratio of ${\~}1:6.8$, xylose and ribose are minor sugar components. The average molecular mass, as determined by gel filtration chromatography, was $2.2{\times}10^3$ KDa, The intrinsic viscosities of EPS-R were calculated to be 16.5 and 15.9 dL/g using the Huggins and Kraemer equations, respectively, with a 2.3 dL/g overlap. In terms of rigidity, the conformation of EPS-R was similar to that of caboxymethyl cellulose ($5.0{\times}10^{-2}$). The rheological behavior of EPS-R dispersion indicated that the formation of a structure intermediate between that of a random-coil polysaccharide and a weak gel. The aqueous dispersion of EPS-R at concentrations ranging from 0.25 to $1.0\%$ (w/w) showed a marked shear-thinning property in accordance with Power-law behavior. In aqueous dispersions of $1.0\%$ EPS-R, the consistency index (K) and flow behavior index (n) were 1,410 and 0.73, respectively. EPS-R was Stable to pH and salts.

• 상하수도학회지
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• 제35권6호
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• pp.379-387
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• 2021

In general, trans-membrane pressure (TMP), flux, filtration resistance are used as indicators to evaluate the degree of fouling in MBR. However, they have limitations in determining the level of EPS generation, which is known as an important factor of fouling. Therefore, a new evaluation method is required to monitor the amount of EPS generation. In this study, the applicability of capillary suction time (CST), which is used to measure the dewaterability of sludge, was evaluated as an indirect fouling evaluation index. Statistical analysis was performed to evaluate the effect of EPS on CST, and to determine whether EPS has high similarity with representative fouling evaluation indicators and CST, and quantitatively compared them. As a result, the correlation coefficient between CST and bEPS was 0.7988, which was higher than the correlation coefficient between filtration resistance and bEPS. Since bEPS is a major factor inducing fouling by affecting the formation of the cake layer, it was evaluated that CST, which has a high correlation with bEPS, is suitable to represent EPS. In addition, it was evaluated that the correlation coefficient between filtration resistance and CST was high as 0.7187, which could be used as a fouling evaluation index.

• 한국자동차공학회논문집
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• 제8권4호
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• pp.186-193
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• 2000

EPS(Electric Power Steering) systems have many advantages over traditional hydraulic power steering systems in space efficiency engine efficiency and environmental compatibility. In this paper an EPS system control logic using a torque map is proposed. The main function of the EPS system is to reduce the steering torque exerted by a driver by assist of an electric motor. Vehcile speed steering torque and steering wheel angle are measured and fed back to the EPS control system where appropriate assist torque is generated to assist the operator's steering effort. Another capability of the EPS system for easy adaptation to different steering feels via simple tuning is demonstrated by the experiments. It will be also verified that the EPS system can also improve damping and return performance of the steering wheel by control of the assist motor.

• 한국포장학회지
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• 제14권1호
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• pp.31-33
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• 2008

The effects of packaging conditions on freshness extension of green pepper were investigated during 30days of storage at $10^{\circ}C$ and $18^{\circ}C$ Corrugated cardboard and EPS box used as packaging materials. The rate of weigh loss, vitamin C and chlorophyll contents, and hunter color of green paper were changed last significantly in the samples stored in the EPS box at low temperature storage condition($10^{\circ}C$) among treatments. The combination of EPS box and low temperature was the most effective treatment to extend the storability of green pepper.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.112-116
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• 2001

The production of extracellular polysaccharide, EPS WN9, from Paenibacillus sp. and its suitability as a viscosity modifying admixture for cement mortar mixing were investigated. After 48h culture in an optimized medium, cell growth and EPS production were 1.2g/L and 4.0g/L, respectively. By adding EPS WN9 to mortar, it was possible to prepare a homogeneous mortar without material segregation and excess air entrapment. The optimal amount of EPS addition to mortar found to be 0.02 to 0.05%(w/w) of the cement used. Increasing the dosage of EPS WN9 from 0 to 0.05%(w/w) resulted in a setting retardation of 0.14h to 0.8h and an increase in the compressive strength of mortar of 10 to 20%.

• 산업공학
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• 제13권2호
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• pp.157-165
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• 2000

In this paper, an efficient method is proposed to place the cooling lines(CLS) and ejector pins(EPS) of mold design. The other components of mold, except CLS and EPS, can be generated automatically by batch processing. But the placements and sizes of CLS and EPS depend on the shapes of a part, so that the design works of CLS and EPS should be processed interactively. Using the pre-defined reference points, the positions of CLS and EPS can be determined interactively. By the proposed method, the interference occurred during placing CLS and EPS can be avoided, and the proper lengths of them can be calculated automatically. The information of the positions and lengths are stored in BOM database for generating a machining data. The proposed method is implemented with Unigraphics API functions and C language, tested on Unigraphics V15.