• Ocean and Polar Research
• /
• 제34권1호
• /
• pp.93-99
• /
• 2012

The effect of water temperature and body weight on oxygen consumption by the fasted olive flounder Paralichthys olivaceus was investigated in order to assess the metabolic rate of this species under different conditions. The oxygen consumption rate (OCR) was measured at three different water temperatures (15, 20 and $25^{\circ}C$) and two different body weights [$9.1{\pm}1.2$ g (mean${\pm}$SD) for the juvenile group and $266.4{\pm}29.3$ g for the immature group] at an interval of 5 minutes for 24 hours using a closed flow-through respirometer. For each treatment condition, three replicates were set up and 135 fish in the juvenile group and 18 fish in the immature group were used. The OCRs exhibited a linear increase described by OCR=-82.06+28.30T ($r^2$=0.96, p<0.001) in the juvenile group and OCR=-52.52+14.73T ($r^2$=0.97, p<0.001) in the immature group. The OCRs decreased with increasing body weights at a given water temperature (p<0.001). The metabolic rate was related to the body weight of the fish as a power function with a weight exponent of between 0.77 and 0.82. $Q_{10}$ values ranged 1.67~2.28 when the temperature was between 15 and $20^{\circ}C$, 1.57~1.93 when the temperature was between 20 and $250^{\circ}C$, and 1.79~1.89 when the temperature was between 15 and $250^{\circ}C$. The energy expenditure by respiration increased with increasing water temperature and decreasing body weight (p<0.001). The mean energy loss rates at 15, 20 and $25^{\circ}C$ were 115.9, 149.8 and 208.2 kJ $kg^{-1}d^{-1}$ in the juvenile groups and 53.8, 81.2 and 101.9 kJ $kg^{-1}d^{-1}$ in the immature groups.

• 고려인삼학회:학술대회논문집
• /
• 고려인삼학회 2002년도 학술대회지
• /
• pp.129-144
• /
• 2002

We investigated anti-hyperglycemic and anti-obese effects of Panax ginseng berry extract and its major constituent, ginsenoside Re, in obese diabetic C57BL/6J ob/ob mice and their lean littermates. Animals received daily intraperitoneal injections of Panax ginseng berry extract for 12 days. On Day 5, 150 mg/kg extract-treated ob/ob mice had significantly lower fasting blood glucose levels compared to vehicle-treated mice $(156{\pm}9.0\;mg/dl\;vs.\;243{\pm}15.8mg/dl,$ P<0.01). On Day 12, the extract-treated ob/ob mice became normoglycemic $(137{\pm}6.7\;mg/dl)$ and had significantly improved glucose tolerance. The overall glucose excursion during the two-hour intraperitoneal glucose tolerance test (IPGTT), calculated as area under the curve (AUC), decreased by $46\%$ (P<0.01) compared to vehicle-treated ob/ob mice. Glucose levels of lean mice were not significantly affected by the extract. The improvement in blood glucose levels in 150 mg/kg extracttreated ob/ob mice was associated with significant reduction in serum insulin levels of fed and fasting mice. Consistent with an improvement in insulin sensitivity, hyperinsulinemic euglycemic clamp study revealed a more than 2-fold increase in the rate of insulin-stimulated glucose disposal in treated ob/ob mice $(112{\pm}19.1\;vs.\;52{\pm}11.8{\mu}mol/kg/min$ for the vehicle group, P<0.01). In addition, 150 mg/kg extract-treated ob/ob mice, but not the lean mice, lost significant weight (from $51.7{\pm}1.9g\;on\;Day\;0\;to\;45.7{\pm}1.2$ on Day 12, P<0.01 compared to vehicle-treated ob/ob mice), associated with a significant reduction in food intake (P<0.05) and a very significant increase in energy expenditure (P<0.01) and body temperature (P<0.01). A 12-day treatment with 150 mg/kg Panax ginseng berry extract also significantly reduced plasma cholesterol levels in ob/ob mice. Additional studies demonstrated that ginsenoside Re, a major constituent of the ginseng berry, but not from the root, plays a significant role in anti-hyperglycemic action. This anti-diabetic effect of ginsenoside Re was not associated with body weight changes, suggesting that other constituents in the extract have distinct pharmacological mechanisms on energy metabolism. The identification of a significant anti-hyperglycemic activity in ginsenoside Re may provide an opportunity to develop a novel class of anti-diabetic agent.

• Journal of Community Nutrition
• /
• 제4권2호
• /
• pp.99-108
• /
• 2002

Recently interests on raw-food diets are rapidly increasing in relation to chronic diseases prevention in Korea, but studies on raw-food diets have been hardly performed by nutritionists. This study was performed to investigate the effects of low-calorie diets using a raw-food formula in the form of freeze-dried powder on obesity and its complications in the obese women (body mass index (BMI) $\geq$ 25kg/㎡) for eight weeks. Forty premenopausal women (mean BMI 28.04kg/㎡, mean age 28.33years old) participated in this diet intervention, and were controlled by eating 1 regular meal, 1-2 snacks and 2 raw-food formula (140kcal/pack) meals a day within the 1500-1300kcal ranges. Anthropo-mentric measurements, body compositions, physical exercise, and obesity-related risk factors were assessed before (the initial), during (the 4th week) and after (the 8th week) the study. All the data was analyzed by paired t-test, repeated measures ANOVA, and nonparametric rank test at p<0.05 level. Obesity was significantly increased during this study, and it was decreased in weight (-4.59%, p<0.000), BMI (-4.56%, p<0.000), body fat percent (-6.18%, p<0.000), fat mass (-10.19%, p<0.000), waist and hip circumferences(-5.69%, p<0.000 and -2.55%, p<0.000) and WHR (-3.24%, p<0.000). Energy expenditure of physical exercise was increased as much as 70kca1/day during the study (p=0.000), but it did not have any correlations with weight loss and changes of body compositions. Biochemical measurements including blood triglyceride(p <0.006) and leptin(p<0.000) levels were significantly decreased, LDL cholesterol level was increased(p<0.05), but all the blood lipid levels were in the normal ranges. Fatty liver echogenicity and menstrual irregularity were improved after the diet intervention(p<0.000 and p<0.034). In conclusion, this B-week low-calorie diet intervention using raw-food formula was effective for obese premenopausal women in reducing obesity and its risk factors so as not to proceed towards comorbidities. However, the variation of blood lipid levels should be observed for a longer Period.

• 대한영양사협회학술지
• /
• 제4권1호
• /
• pp.65-75
• /
• 1998

Recently, the number of patients who received Bone Marrow Transplantation(BMT) has been increased dramatically and the diseases for which BMT if efficacious are increasing. Adequate nutritional card for BMT patients is crucial for the success of BMT because nutritional deficiency could provoke deteriorative effects. However, little is known about nutritional status among BMT patients in Korea. This study was conducted to assess oral and parenteral intake of BMT patients and compare the change of nutritional status before and after BMT. Twenty-two BMT patients who were admitted to the Severance hospital from December in 1995 to September in 1997 participated in the study. Total calorie requirements were calculated for each patients individually and nutritional support for each patients consisted of oral and parenteral feeding. To assess oral intake of BMT patients, each patients recorded the amount of food they have eaten from 6 days before BMT to 28 days after BMT. The medical records of each patients were used to assess parenteral intake. To compare the nutritional status before and after BMT, the results of anthropometric and biochemical test from 14 days before BMT to 28 days after BMT were used. At the time of admission, the patients were in allowable nutritional status and their total calorie intake was 93% of total calorie requirement. When the preparative regimen for BMT was started, the patients' oral intake was dramatically decreased below 400kcal/day. Even though their oral intake was increased after BMT, their oral intake at the 4th week after BMT was only 752kcal/d, which is only 35.8% of total calorie requirement. The patients' mean oral intake during BMT period (from 6 days before BMT to 28 days after BMT) was only 439kcal/d. Although Total Parenteral Nutrition(TPN) was added when the preparative regimen was started, the patients' mean total caloric intake during BMT period was 111% of basal energy expenditure and 83% of total calorie requirement. The mean total protein intake was only 58% of total protein requirement. In the comparison of nutritional status between pretransplant phase and posttransplant phase in BMT patients, their body weight and serum albumin level were significantly decreased(p<0.001). These results show inadequacies in nutritional intake among BMT patients, and indicate the need of TPN during BMT period.

• Korean Chemical Engineering Research
• /
• 제56권4호
• /
• pp.496-523
• /
• 2018

본 연구의 목표는 톱밥의 건조, 급속열분해, 바이오오일 응축, 바이오오일 수첨개질, 전기생산, 폐수처리를 포함하는 2개의 바이오오일 생산공정들에 대한 경제적 타당성을 평가하는 것이다. 첫번째 공정은 수소생산을 위한 steam-methane reforming (SMR)을 포함하는 바이오오일 생산공정이다(Case 1). 두번째 공정은 SMR을 포함하지 않고 수소를 외부로부터 공급받아 수첨개질을 수행하는 바이오오일 생산공정이다(Case 2). 상용공정모사기인 ASPEN Plus를 이용하여 이두 공정에 대한 공정흐름도를 구축하였고, 물질 및 에너지 수지식을 계산하였다. 원료로서 40%의 수분을 포함하는 톱밥 100 t/d, 30% 자기자본비율, 자기자본에 상응하는 자본지출, 수소 구입가 $1,050/ton, 완전건조된 원료대비 수송용 연료 수율 20% (Case 1) 및 25% (Case 2) 로 가정하고, 4단계 경제성 분석기법인 4-level EP를 사용하여 기술경제성 분석을 수행하였다. 총투자비(TCI), 총생산비(TPC), 연간판매금액(ASR), 그리고 개질 바이오오일의 최소판매가격(MFSP)은 Case 1에 대하여 $22.2 million, $3.98 million/yr, $4.64 million/yr, 그리고 $1.56/l 이고, Case 2에 대하여 $16.1 million, $5.20 million/yr, $5.55 million/yr, $1.18/l로 산출되었다. 수소를 직접 생산하는 Case 1과 수소를 외부로 부터 공급받는 Case 2의 투자회수율(ROI)와 투자회수기간(PBP)은 큰 차이를 보여주지 않았고, Case 1과 Case 2의 원료 공급량을 1,500 t/d로 증가할 경우, ROI는 15% 이상으로 향상될 것으로 예상되었다.

• Journal of Nutrition and Health
• /
• 제4권1호
• /
• pp.49-62
• /
• 1971

한국인(韓國人)의 기초대사량(基礎代謝量)과 1일소비열량(日消費熱量)을 측정(測定)하기 위하여 Douglas씨(氏) ${\ulcorner}$빽${\lrcorner}$과 Scholander씨(氏) ${\ulcorner}$가스${\lrcorner}$ 분석기(分析器)를 사용(使用)하여 넓은 연령(年齡), 직종(職種), 활동(活動)의 영역(領域)에 긍(亘)하여 실험(實驗)을 행(行)하였다. 938 예(例)의 기초대사측정치(基礎代謝測定値)는 Boothby, Fleish, Robertson and Reid 등(等)의 표준치(標準値)와 근사(近似)하였다. 겨울에는 기초대사량(基礎代謝量)은 9.9%의 증가(增加)를 또한 수면중(睡眠中)의 대사량(代射量)은 기초대사량(基礎代謝量)의 약(約) 94%이었다. 또한 Sanborn Metabulator를 사용(使用)하여 1,000 명(名) 이상(以上)의 기초대사량(基礎代謝量)을 측정(測定)하였다. 또한 여러 가지 동작(動作) 활동중(活動中)의 소비열량(消費熱量)이 측정(測定)되었다. 하루의 전소비열량(全消費熱量)은 $3{\sim}7$일간(日間)에 긍(亘)하여 하루 24시간(時間)의 활동상황(活動狀況)을 분단위(分單位)로 기록(紀錄), 집계(集計)하고 이것과 기초대사량(基礎代謝量) 및 개별활동시(個別活動時)의 소비열량(消費熱量)을 참작(參酌), 계산(計算)하였다. 수면중(睡眠中)의 소비열량(消費熱量)은 기초대사량(基礎代謝量)에 준(準)하였는데 사무원(事務員), 교원(敎員), 의사(醫師), 약사(藥師), 점원(店員), 운전수(運轉手), 건축기사(建築技師), 설계사(設計師), 응원사(應園師), 목공(木工), ${\ulcorner}$펜기${\lrcorner}$ 공등(工等)의 1일소비열량(日消費熱量)은 FAO 한국협회책정(韓國協會策定)의 한국인(韓國人) ${\ulcorner}$칼로리${\lrcorner}$에 근사(近似)하였고 ${\ulcorner}$쿡${\lrcorner}$, ${\ulcorner}$웨이터${\lrcorner}$, 농부(農夫), 사공(士工), 기계공(機械工), 석공(石工)은 중등정도(中等程度)의 노동(勞動)의 3,000 ${\ulcorner}$칼로리${\lrcorner}$나 또는 중(重)한 노동(勞動)의 3,900 ${\ulcorner}$칼로리${\lrcorner}$에, 가정주부(家政主婦), 하녀(下女), ${\ulcorner}$디자이너${\lrcorner}$는 여자(女子)의 중등정도(中等程度) 노동(勞動)의 2,200 ${\ulcorner}$칼로리${\lrcorner}$, 간호원(看護員), 청소부(淸掃婦), ${\ulcorner}$쿡${\lrcorner}$ 방(房)바닥연마공(硏磨工)은 중(重)한 노동(勞動)의 2,800${\ulcorner}$칼로리${\lrcorner}$에 모심기 하는 날의 농부(農夫)는 격심(激甚)한 노동(勞動)의 4,100${\ulcorner}$칼로리${\lrcorner}$에 각각(各各) 가까웠다. 여자(女子)일지라도 포제용(鋪製用) 돌까기와 돌날르기는 남자(男子)의 중노동정도(重勞動程度)의 ${\ulcorner}$칼로리${\lrcorner}$를 소비(消費)하였다.

• Journal of Nutrition and Health
• /
• 제31권4호
• /
• pp.729-738
• /
• 1998

Antioxidants such as vitamin C and E may play a preventive role in the development of cancer and coronary heart disease. The status of vitamins C and E may be affected by lifestyle habits such as smoking , drinking, and exercise. These habits can modify the dietary requirements of vitamin C and vitamin E. the purpose of this study was to determine whether Korean young healthy men and women consume vitamins C and E in sufficient quantities relative to their lifestyle habits. Among the participants in this study, 52% of the men and none of the women were smokers. ; 84% of all subjects drank alcohol more than once a week ; and the men exercised more often than the women. The concentrations of serum total , HDL-, and LDL- cholesterol were higher in the women than in the men, but the serum triglyceride concentrations were higher in the men than in the women. The men consumed less satuated fat than the women (p<0.05) . The daily intakes of vitamin C for the men and the women were 47.1mg and 65.6mg, respectively. On the other hand , the daily vitamin E intake was higher in the men (11.8mg) than women(6.9mg). The serum $\alpha$-tocopherol concentrations of all subjects were in a normal range, and in no subjects were they below the minimum value of ranges. However, about 19% of male subjects and 10% of female subjects showed deficient status, although the mean serum vitamin C levels were normal . Lifestyle habits fo the sort mentioned above have little influence on the serum vitamin C and $\alpha$-tocopherol concentrations. The serum $\alpha$-tocopherol concentration had a positive correlation with total fat and alcohol consumption. The serum vitamin C concentration was positively associated with regular exercise, but it was negatively correlated with the number of cigarettes smoked. Meanwhile, the serum lipid persoxide concentration , the indirect index of oxidative stress, was influenced by certain variable such as body mass index , the number of cigarettes smoked , alcohol consumption, energy expenditure, vitamin C intake, and serum ${\gamma}$-tocopherol concentration. Serum lipid peroxide concentration was positively associated with body mass index, the number of cigarette smoked , serum triglyceride , and HDL-cholesterol concentration. In conclusion , the vitamin E requirements of the subjects were met by the Recommended Daily Allowance (RDA) regardless of lifestyle habits. However, serum vitamin C concentrations showed individual variation and was below the normal ranges. Smoking and exercise influenced serum vitamin C concentration. Therefore, a reevaluation of the requirements of vitamin C relative to lifestyle habits is necessary.

• 대한본초학회지
• /
• 제27권2호
• /
• pp.53-59
• /
• 2012

Objectives : This study was undertaken to investigate the effects of the GGEx18 ethyl acetate fraction (EF) on lipid accumulation and gene expression of fatty acid-oxidizing enzymes using 3T3-L1 adipocytes, C2C12 skeletal muscle cells, and NMu2Li liver cells. Methods : PPAR${\alpha}$, AMPK and UCPs transactivation was examined in NMu2Li hepatocytes, C2C12 myocytes, and 3T3-L1 preadipocytes using transient transfection assays. Results : 1. Compared with control, EF significantly increased the mRNA expression of VLCAD in 3T3-L1 adipocytes. 2. Compared with control, EF (0.1 ${\mu}g/ml$) significantly inhibited lipid accumulation in 3T3-L1 adipocytes. 3. EF significantly increased the mRNA expression of AMPK${\alpha}$1, AMPK${\alpha}$2 and PPAR${\alpha}$ in C2C12 skeletal muscle cells compared with control. 4. EF significantly increased the mRNA expression of genes involved in fatty acid ${\beta}$-oxidation, such as thiolase, MCAD, and CPT-1 in C2C12 skeletal muscle cells compared with control. 5. EF significantly increased the mRNA expression of UCP2 involved in energy expenditure in C2C12 skeletal muscle cells compared with control. 6. Compared with control, EF (10 ${\mu}g/ml$) significantly inhibited lipid accumulation in C2C12 skeletal muscle cells. 7. EF (10 ${\mu}g/ml$) significantly increased the mRNA expression of ACOX, HD, VLCAD and MCAD in NMu2Li liver cells compared with control. Conclusions : These results suggest that EF may prevent obesity by increasing the mRNA expression of mitochondrial fatty acid ${\beta}$-oxidizing enzymes in 3T3-L1 adipocytes, by not only regulating the fatty acid oxidation through activation of AMPK and PPAR${\alpha}$, but also increasing the UCP2 mRNA expression in C2C12 skeletal muscle cells, and by stimulating the mRNA expression of fatty acid-oxidizing enzymes in NMu2Li liver cells.

• 전자공학회논문지
• /
• 제52권7호
• /
• pp.135-142
• /
• 2015

인체 구동 방식의 자가 발전기는 외부 환경에 덜 의존한다는 장점 덕분에 비상용 혹은 야외 활동 중의 소규모 전력 공급원으로 주목 받고 있다. 하지만 대부분의 자가 발전기는 전자기 유도 결합의 효율이 높지 않아 출력이 미미하며, 특정 부위의 근육만을 지속적으로 사용하는 단조로운 구동 방식을 택하고 있어, 사용 중에 쉽게 지루해질 뿐만 아니라 근피로가 쉽게 누적되어 실제 원하는 출력을 얻을 만큼 충분한 시간 동안 구동하는 것이 거의 불가능하다. 이러한 낮은 효율과 사용성의 문제로 인해 인체 구동 방식의 자가발전기가 실생활에서 사용되는 빈도는 높지 않다. 본 연구에서는 전자기 유도 효율이 높은 종축 결합 방식의 발전 장치를 간단한 구조로 구현하여 출력을 높이는 동시에, 자성체의 운동 관성에 의한 가속이 가능한 인체 구동 방식의 발전기를 설계 제작하였다. 개발된 발전기와 기존 상용 자가 발전기와의 비교 실험을 통해 높은 출력 뿐 아니라 장시간 구동이 가능함을 검증하였다. 아울러 인체 구동 발전기에서 실질적으로 중요한 요소임에도 불구하고 기존 연구에서 제시된 바 없는 인체의 투입 에너지를 실증적으로 파악하기 위해 호흡 가스 기반의 실제 운동 대사량 측정법에 근거한 실험을 시행하여 자가 발전기의 운동 부하를 추정하였다. 향후 성능 개선과 휴대성 증진을 통해 모바일 기기의 전력 지속 문제를 해결하는 데 기여할 수 있을 것으로 기대한다.

• Toxicological Research
• /
• 제30권2호
• /
• pp.91-97
• /
• 2014

This study aimed to identify changes in the level of neuropeptides among current smokers, former smokers, and individuals who had never smoked, and how smoking habits affect obesity and metabolic syndrome (MetS). Neuropeptide levels, anthropometric parameters, and metabolic syndrome diagnostic indices were determined among male workers; 117 of these had never smoked, whereas 58 and 198 were former and current smokers, respectively. The total sample comprised 373 male workers. The results obtained from anthropometric measurements showed that current smokers attained significantly lower body weight, body mass index, waist circumference, and abdominal fat thickness values than former smokers and those who had never smoked. Current smokers' eating habits proved worse than those of non-smokers and individuals who had never smoked. The level of brain-derived neurotrophic factor (BDNF) in the neuropeptides in the case of former smokers was $23.6{\pm}9.2pg/ml$, higher than that of current smokers ($20.4{\pm}6.1$) and individuals who had never smoked ($22.4{\pm}5.8$) (F = 6.520, p = 0.002). The level of adiponectin among former smokers was somewhat lower than that of current smokers, whereas leptin levels were higher among former smokers than current smokers; these results were not statistically significant. A relationship was found between adiponectin and triglyceride among non-smokers (odds ratio = 0.660, ${\beta}$ value=-0.416, p < 0.01) and smokers (odds ratio = 0.827, ${\beta}$ value=-0.190, p < 0.05). Further, waist circumference among non-smokers (odds ratio = 1.622, ${\beta}$ value=0.483, p < 0.001) and smokers (odds ratio = 1.895, ${\beta}$ value=0.639, p < 0.001) was associated with leptin. It was concluded that cigarette smoking leads to an imbalance of energy expenditure and appetite by changing the concentration of neuropeptides such as adiponectin, BDNF, leptin, and hsCRP, and influences food intake, body weight, the body mass index, blood pressure, and abdominal fat, which are risk factors for MetS and cardiovascular disease.