• Asian-Australasian Journal of Animal Sciences
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• v.16 no.12
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• pp.1705-1710
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• 2003

A small body size of Brown Tsaiya laying duck is desirable to reduce maintenance requirements, so the body weight at 40 weeks of age (BW40) has to be maintained at its current level. Egg weight has to be maintained at around 65 g to meet market requirements. Eggshell strength at 40 weeks of age (ES40) must to be increased in order to maintain a low incidence of broken eggs. Thus, number of eggs laid up to 52 weeks of age (EN52) has to be increased without negative correlated response on ES40. A new linear genetic selection index was used: $I_g=a_0{\times}GEW40\;(g)+a_1{\times}GBW40\;(g)+a_2{\times}GES40\;(kg/cm^2)+a_3{\times}GEN52\;(eggs)$ where GEW40, GBW40, GES40 and GEN52 were the multitrait best linear unbiased prediction (MT-BLUP) animal model predictors of the breeding values respectively of egg weight and body weight at 40 weeks of age (EW40, BW40), ES40 and EN52. The coefficients $a_0$, $a_1$, $a_2$ and $a_3$ were calculated with constraints of 0.0 g, 0.0 g and $0.013kg/cm^2$ for expected genetic gains in EW40, BW40 and ES40 respectively and maximum gain in EN52. Since 1997, the drakes and the ducks were selected according to their own indexes, with this new genetic selection index. From G0 to G4, the average per generation predicted genetic responses in female duck were +0.05 g for EW40, +0.92 g for BW40, $+0.035kg/cm^2$ for ES40 and +2.13 eggs for EN52. Which represented respectively 0.07%, 0.06%, 0.67% and 1.0% of the means of the EW40, BW40, ES40 and EN52. For ES40 and EN52, it represented also respectively 16.1% and 21.6% of the additive genetic standard deviation of these traits. Thevse results indicated that selection of laying Brown Tsaiya by a restricted genetic selection index and with MT-BLUP animal model could be an efficient tool for improving the efficiency of egg production, increasing egg shell strength and egg number while holding egg weight and body weight constants.

• The Korean Journal of Mycology
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• v.25 no.1 s.80
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• pp.10-19
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• 1997

Fusarium wilt of radish (Raphanus sativus L.) is caused by the Fusarium oxysporum f. sp. raphani (FOR) which mainly attacks Raphanus spp. The pathogen is a soil-borne and forms chlamydospores in infected plant residues in soil. Infected pathogen colonizes the vascular tissue, leading to necrosis of the vascular tissue. Growth promoting beneficial organisms such as Pseudomonas fluorescens WCS374 (strain WCS374), P. putida RE10 (strain RE10) and Pseudomonas sp. EN415 (strain EN415) were used for microorganisms-mediated induction of systemic resistance in radish against Fusarium wilt. In this bioassy, the pathogens and bacteria were treated into soil separately or concurrently, and mixed the bacteria with the different level of combination. Significant suppression of the disease by bacterial treatments was generally observed in pot bioassy. The disease incidence of the control recorded 46.5% in the internal observation and 21.1% in the external observation, respectively. The disease incidence of P. putida RE10 recorded 12.2% in the internal observation and 7.8% in the external observation, respectively. However, the disease incidence of P. fluorescens WCS374 which was proved to be highly suppressive to Fusarium wilt indicated 45.6% in the internal observation and 27.8% in the external observation, respectively. The disease incidence of P. putida RE10 mixed with P. fluorescens WCS374 or Pseudomonas sp. EN415 was in the range of 10.0-22.1%. On the other hand, the disease incidence of P. putida RE10 mixed with Pseudomonas sp. EN415 was in the range of 7.8-20.2%. The colonization by FOR was observed in the range of $2.4-5.1{\times}10^3/g$ on the root surface and $0.7-1.3{\times}10^3/g$ in the soil, but the numbers were not statistically different. As compared with $3.8{\times}10^3/g$ root of the control, the colonization of infested ROR indicated $2.9{\times}10^3/g$ root in separate treatments of P. putida RE10, and less than $3.8{\times}10^3/g$ root of the control. Also, the colonization of FOR recorded $5.1{\times}10^3/g$ root in mixed treatments of 3 bacterial strains such as P. putida RE10, P. fluorescens WCS374 and Pseudomonas sp. EN415. The colonization of FOR in soil was less than that of FOR in root part. Based on soil or root part, the colonization of ROR didn't indicate a significant difference. The colonization of introduced 3 fluorescent pseudomonads was observed in the range of $2.3-4.0{\times}10^7/g$ in the root surface and $0.9-1.8{\times}10^7/g$ in soil, but the bacterial densities were significantly different. When growth promoting organisms were introduced into the soil, the population of Pseudomonas sp. in the root part treated with P. putida RE10 was similar in number to the control and recorded the low numerical value as compared with any other treatments. The population density of Pseudomonas sp. in the treatment of P. putida RE10 indicated significant differences in the root part, but didn't show significant differences in soil. The population densities of infested FOR and introduced bacteria on the root were high in contrast to those of soil. P. putida RE10 and Pseudomonas sp. EN415 used in this experiment appeared to induce the resistance of the host against Fusarium wilt.

• YAKHAK HOEJI
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• v.47 no.3
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• pp.159-166
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• 2003

In order to study the clinical usefulness of crude polysaccharides fractionated from Eleutherococcus senticosus, EN-3, in eliminating tumors, we have investigated the effect of combination therapy on the murine tumor metastasis and growth models. In experimental metastasis of colon26-M3.1 cells, prophylactic intravenous (i.v.) administration of EN-3 (0.5, 5, and 50 $\mu\textrm{g}$/mouse) inhibited tumor metastasis compared with tumor control group in 33.6, 66.8, and 81.8％ respectively. The administration of EN-3 (50 $\mu\textrm{g}$/mouse) also exhibited a 66.1％ therapeutic effect on lung tumor metastasis. Although EN-3 induced no toxic effect on both tumor cell and normal splenocyte in the concentration below 100 $\mu\textrm{g}$/mι in in vitro, it induced significant proliferating activity on normal splenocyte in the concentration-dependent manner. In an analysis of NK-cell activity, i.v. administration of EN-3 (4∼100 $\mu\textrm{g}$/mouse) significantly augmented NK cytotoxicity to YAC-1 tumor cells. The combination treatments of cisplatin (10 $\mu\textrm{g}$) and EN-3 (5 $\mu\textrm{g}$) induced synergistic effect on the inhibition of tumor metastasis in experimental tumor metastasis model produced by colon26-M3.1 cells. In addition, the combination treatments also exhibited prolongation of lifespan in S∼180 tumor bearing mouse for over the 60 days. Even though cisplatin (2.5 $\mu\textrm{g}$/mι) exhibited cytotoxicity to tumor cells and inhibited tumor growth over 95％ in in vitro, combination treatment with EN-3 (20 $\mu\textrm{g}$/mι) was induced splenocyte proliferation and produced cytokines, such as TNF-$\alpha$, IL-1 and IL-12, from the macrophages. These results suggested that EN-3 stimulate immune system non-specifically and apply to the biological response modifiers (BRM) in chemo-immunotherapy for tumor prevention.

• Korean Journal of Poultry Science
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• v.11 no.2
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• pp.86-92
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• 1984

Present study was carried out to estimate phenotypic and genetic parameters influencing body weight (BW) at 4 weeks of age egg breadth (EB), egg length(EL), egg shape index (SI) and egg weight (EW) at 32 weeks of age and egg numbers (EN) up to 38 weeks of age in broiler male and female lines. The data were collected from closed White Plymouth Rock (female line; G) and Cornish (male line; C) flocks involving 1193 pullets from 211 dams and 48 sires in 1982. The results obtained are summarized as follow: 1. General performance for various trails of lines C and G. The means and standard deviations of BW, EB, EL, SI, EW and EN were 668.34${\pm}$47.18, 4.23${\pm}$0,11, 5.49 ${\pm}$0.19, 77.06${\pm}$2.98, 55.73${\pm}$3.54 and 59.72${\pm}$13.39 in line C, respectively and 487.89${\pm}$ 41.43, 4.22${\pm}$0.11, 5.51${\pm}$0.19, 76.72${\pm}$3.20, 55.43${\pm}$3.26 and 76.93${\pm}$12.17 in line G, respectively. 2. Heritability Heritabilities were estimated from sire, dam and combined components. Estimates for BW, EB, EL, SI, EW and EN from combined components were 0.30, 0.29, 0,40, 0.22, 0.45 and 0.60 in line C, respectively and 0.33, 0.23, 0.28, 0.13, 0.49 and. 0.33 in line G, respectively. 3. Correlation Genetic and phenotypic correlations showed similar trend in line C and G. Genetic correlations, estimated EW with EB and EL, were high and positive (line C; 0.99, 0.75, respectively and line G; 0.94, 0.82, respectively), also correlation of EB with EL was 0.58 (both lines; 0.58). High and negative genetic correlations were shown between SI and EL in line C and G (-0.70, -0.65, respectively). Genetic correlations between SI and EW were relatively low and negative in line C and G (-0.11, -0.19, respectively) and between SI and EN were relatively low and positive in line C and G (0.25, 0.17, respectively). Between other traits, low genetic correlations were shown in both lines, High and positive correlation was estimated between hatchability and egg shape index and polynomial regression of egg shape index on hatchability was estimated; Y=-216.77＋7.6216X-0.0146939X$^2$.

• Korean Journal of Food Science and Technology
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• v.35 no.6
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• pp.1209-1215
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• 2003

This study was carried out to establish a quantitative analysis method of separating immuno-activating substance (EN-SP) from Acanthopanax senticosus (A. senticosus) by competitive direct ELISA. Mouse antiserum (anti-EN-SP) against EN-SP was generated by immunization (s.c.) of EN-SP purified from A. senticosus as an immunogen. The titer of anti-EN-SP was about 1 : 400, and the optimal dilution of EN-SP-HRP conjugate was 1 : 1,000. When the standard curve was constructed by ELISA, its sensitivity was about $0.2{\mu}g/mL$. The coefficient variation of intra- and inter-assay were $6.13{\sim}8.81%$ and $6.73{\sim}8.60%$, respectively. According to the standard curve, the concentration of EN-SP in various senticosus extracts was found to be only $59.85\;{\mu}g$ in 10mg of extract from the bark of A. senticosus. Similarly, the immunostimulating activity to produce $TNF-{\alpha}$ or IL-12 among the various extracts of Acanthopanax was shown to be correlated with the content of EN-SP. These results demonstrated that competitive ELISA was a convenient, fast, reproducible, and accurate method for the determination of EN-SP as an immunologically active standard substance in extract of A. senticosus.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.16 no.2
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• pp.189-211
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• 2003

The clinical data and thermographic imaging were analyzed on the 38 cases of Bell's palsy who were treated admission in the Oriental Medicine Hospital of Wonkwang University from January 2002 to May 2003. 38 patients with Bell's palsy were within one week after the onset of the paralysis, and thermal type in the DITI were hypo or hyper generally. Nerve conduction test(ENOG and EMG) examined in two weeks after onset. We studied interaction effect between thermal type and paralysis grade on admission day. We studied each main effect ; paralysis grade on admission day - nerve conduction test, nerve conduction test - thermal type, thermal type - paralysis grade after 4 weeks, paralysis grade on admission day - paralysis grade after 4 weeks, sasang constitution - nerve conduction test. The following results were obtained that interaction effect between thermal type and paralysis grade on admission day showed no significance, each main effect ; paralysis grade on admission day - nerve conduction test, paralysis grade on admission day - paralysis grade after 4 weeks, showed significance, each main effect ; nerve conduction test - thermal type, thermal type - paralysis grade after 4 weeks, sasang constitution - nerve conduction test, showed no significance.

• Mycobiology
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• v.35 no.3
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• pp.145-149
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• 2007

The Elfvingia applanata (EA), Hericium erinaceum (HE), Grifola frondosa (GF), Pholiota nameko (PN), Pleurotus eryngii (PE), Trametes suaveolens (TS), Fomes fomentarius (FF), and Inonotus obliquus (IO) could produce the endo- (EN) and exo-biopolymer (EX) in submerged culture. The highest anti-complementary activity of the EN was exhibited by PN (49.1%), followed by HE (38.6%), TS (37.0%), and FF (33.0%), whereas the high activity of the EX was found with GF (59.8%), followed by HE (36.3%), TS (30.8%), and IO (28.8%). The EN of P. nameko (EN-PN) and EX of G. frondosa (EX-GF) were found to contain 78.6% and 41.2% carbohydrates, while 21.4% and 58.8% protein, respectively. The sugar and amino acid compositions of EN-PN and EX-GF were also analyzed in detail.

• The Korean Journal of Malacology
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• v.30 no.4
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• pp.371-381
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• 2014

The bioaccumulation of 16 United States Environmental Protection Agency (USEPA) priority polycyclic aromatic hydrocarbons (PAHs) and alkylated PAHs in the Manila clam, Ruditapes philippinarum exposed to sediments artificially contaminated by Iranian Heavy Crude Oil was measured and the biota-sediment accumulation factor (BSAF) was estimated through laboratory experiments. The proportion of 16 PAHs accumulated in the tissue of R. philippinarum was only from 3 to 7% of total PAHs. Among 16 PAHs, the concentration of naphthalene was highest in the tissue. Alkylated PAHs were highly accumulated more than 93% of total PAHs. The C3 dibenzothiophene was most highly accumulated. The relative composition of alkylated naphthalenes in the tissue of R. philippinarum was lower than in the sediments. In contrast, those of alkylated compounds of fluorenes, phenanthrenes, dibenzothiophenes were higher in the tissue than the sediments. The BSAF for sum of 16 PAHs was 0.11 to 0.13 g carbon/g lipid and that for alkylated PAHs was 0.05 to 0.06 g carbon/g lipid. Naphthalene showed the highest BSAF value. Alkylated PAHs with the same parent compound, BSAF tended to increase with the number of alkylated branch increased, except for alkylated chrysenes. BSAF of total PAHs lies between that of field-based values, and are also similar to those of other persistent organic pollutants (PCBs, DDTs, HCHs). This study provides the BSAF values of individual alkylated PAHs accumulated in R. philippinarum for the first time and will be used as a basis for further understanding the bioaccumulation of organic contaminants in the marine benthic organisms.

• Korean Journal of Poultry Science
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• v.19 no.4
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• pp.187-195
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• 1992

This study carried out to investigate selection response of pure line population of layer chicken. Data for two layer pure line of Single Comb White Leghorn were collected from 1986 to 1991. Primary selection criteria were egg number to 270 days of age (EN270), egg weight(BW270), body weight (BW270) at 270 days of age and the days of the 1st egg(SM). The results obtained in this study are summarized as follows ; 1. Mean phenotypic values of two strains were decreased by 1.94 day(K), 1.320ay(F) for SM and increased by 0.09g(K), -0.05g(F) for EW 270 and 0.56(K), -0.78(F) for EN270. 2. The ratio of a realized selection differential to expected selection differentials for SM (sexual maturity), EW 270, and BW 270 were not significantly decreased during 5 generations. 3. Heritability were estimated from sib analysis. The means of estimates were 0.28(F), 0.39(K) for BW270, and 0.25(F), 0.29(K) for EN270 and 0.51(F), 0.48(K) for EW270. 4. The genetic gains were estimated by method of selection index in retrospect on the basis of selection differentials. EN270 and EW270 were found to increase by 2.01(F), 2.25(K) and 0.54 (F), 0.65(K) per generation.

• Journal of Integrative Natural Science
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• v.7 no.3
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• pp.188-192
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• 2014

Determination of cobalt(III) ion with a perfluorinated sulfonated polymer-ethylenediamine (nafion-en) modified glassy carbon electrode is studied. It is based on the chemical reactivity of an immobilized layer, nafion-en, to yield complex $[Co(en)_3]^{3+}$. The reduction peak potential by differential pulse voltammetry (DPV) is observed at $-0.437{\pm}0.047$ V (vs. Ag/AgCl). The linear calibration curve is obtained in cobalt(III) ion concentration range $1.0{\times}10^{-8}{\sim}1.0{\times}10^{-3}M$ ($5.893{\times}10^{-12}{\sim}5.893{\times}10^{-5}g/mL$).