• Title/Summary/Keyword: ELF

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Endolichenic Fungal Community Analysis by Pure Culture Isolation and Metabarcoding: A Case Study of Parmotrema tinctorum

  • Yang, Ji Ho;Oh, Seung-Yoon;Kim, Wonyong;Hur, Jae-Seoun
    • Mycobiology
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    • v.50 no.1
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    • pp.55-65
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    • 2022
  • Lichen is a symbiotic mutualism of mycobiont and photobiont that harbors diverse organisms including endolichenic fungi (ELF). Despite the taxonomic and ecological significance of ELF, no comparative investigation of an ELF community involving isolation of a pure culture and high-throughput sequencing has been conducted. Thus, we analyzed the ELF community in Parmotrema tinctorum by culture and metabarcoding. Alpha diversity of the ELF community was notably greater in metabarcoding than in culture-based analysis. Taxonomic proportions of the ELF community estimated by metabarcoding and by culture analyses showed remarkable differences: Sordariomycetes was the most dominant fungal class in culture-based analysis, while Dothideomycetes was the most abundant in metabarcoding analysis. Thirty-seven operational taxonomic units (OTUs) were commonly observed by culture-and metabarcoding-based analyses but relative abundances differed: most of common OTUs were underrepresented in metabarcoding. The ELF community differed in lichen segments and thalli in metabarcoding analysis. Dissimilarity of ELF community intra lichen thallus increased with thallus segment distance; inter-thallus ELF community dissimilarity was significantly greater than intra-thallus ELF community dissimilarity. Finally, we tested how many fungal sequence reads would be needed to ELF diversity with relationship assays between numbers of lichen segments and saturation patterns of OTU richness and sample coverage. At least 6000 sequence reads per lichen thallus were sufficient for prediction of overall ELF community diversity and 50,000 reads per thallus were enough to observe rare taxa of ELF.

Analysis and Detection of Linux ELF File Viruses (리눅스 ELF 파일 변형 바이러스의 분석 및 탐지)

  • 곽효승;김두현;김판구
    • Proceedings of the Korean Information Science Society Conference
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    • 2001.10a
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    • pp.781-783
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    • 2001
  • 본 논문에서는 리눅스의 대표적인 파일 포맷인 ELF 파일의 구조에 대해서 알아보며 리눅스 ELF 파일 변형 바이러스의 패턴을 추출하기 위해서 몇몇의 ELF 파일 변형 바이러스를 분석해보고 이것을 바탕으로 패턴을 추출하였다. 바이러스를 탐지하기 위한 방법으로는 전체 검색법과 특정위치 검색법이 있는데 본 논문에서는 특정위치 검색법을 사용하여 ELF 파일 변형 바이러스를 탐지하는 시스템을 구성하였다. 본 논문에서 제안한 바이러스 탐지시스템은 리눅스 ELF 파일 변형 바이러스 탐색을 위해 대상 ELF 파일을 열어서 ELF 파일 여부를 확인하고, 그 파일의 헤더와 프로그램 헤더 부분을 읽어들인 다음 특정 위치를 추적해 바이러스 코드 패턴의 유무를 판별하도록 하였다. 시스템 구현결과 리눅스 ELF 파일 변형 바이러스도 기존의 Windows 계열 파일 변형 바이러스와 비슷한 행위를 수행함을 확인하였다.

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Biological effects of electromagnetic fields at extremely low frequencies (극저주파(Extremely Low Frequency : ELF) 대 전자기장(Electromagnatic field)의 생물학적 영향)

  • Chung, Hai-Won
    • Journal of Radiation Protection and Research
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    • v.20 no.3
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    • pp.187-200
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    • 1995
  • This paper reviews studies on potential health effects of electric and magnetic fields at extremely low frequencies(ELF). Recent studies focus on the epidemiological investigation of cancer, reproductive outcome and neurobehavioral effects due to residential and occupational exposure to ELF. Laboratory experiments are also carried out to find the possible mechanism of biological effects of ELF. Firm conclusion regarding whether ELF cause cancer are not yet conclusive but suggestive and other health effects of exposure to ELF have also been suggested but the evidence is even weaker than that related to cancer.

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Review on the Association between Exposure to Extremely Low Frequency-Magnetic Fields (ELF-MF) and Childhood Leukemia (극저주파 자기장의 소아백혈병 발생 위험 고찰)

  • Dong-Uk Park
    • Journal of Environmental Health Sciences
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    • v.49 no.2
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    • pp.57-65
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    • 2023
  • Background: The association between exposure to extremely low frequency-magnetic fields (ELF-MF) and childhood leukemia has been controversial. There is a need to clarify this relationship by summarizing key conclusions from systematic review articles. Objectives: The major aim of this study is to summarize key conclusions from systematic review articles on the association between exposure to ELF-MF and childhood leukemia based on childhood exposure to ELF-MF, proximity from childhood household to high voltage cables, and parental occupational exposure to ELF-MF. Methods: This study was conducted through a brief literature review focusing on systematic, meta-analysis, and pooled analysis methods. We conducted a literature search in PubMed using the key words "ELF-MF" and "childhood leukemia" singly or combined. Results: In 2002, the World Health Organization (WHO)'s International Agency for Research on Cancer (IARC) reviewed two manuscripts to conduct pooled analysis and concluded that there is a significant association between exposure to >0.3 μT or 0.4 μT and childhood leukemia. We found a total of four manuscripts for systematic or pool analysis that have been published since the IARC's conclusion. They consistently concluded that there was a significant association between exposure to >0.4 μT and childhood leukemia compared to ELF-MF exposure to below 0.1 μT. The proximity of children's households to high voltage cable lines and occupational exposure by their parents to ELF-MF during certain periods prior to or during pregnancy were inconsistently associated with childhood leukemia. The study found that many EU countries have implemented precautionary policies to prevent potential childhood leukemia due to exposure to ELF-MF. Conclusions: This study recommends implementing a precautionary policy that includes legal exposure limits for ELF-MF to minimize exposure to ELF-MF.

Brief Review on Exposure Characteristics, Monitoring Instruments and Threshold Limit Values for Extremely Low Frequency-Magnetic Field (ELF-MF) (직업성 극저주파 자기장 노출평가와 노출 기준에 대한 쟁점 고찰)

  • Dong-Uk, Park;Seunghee, Lee;Kyung Ehi, Zoh
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.32 no.4
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    • pp.381-392
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    • 2022
  • Objectives: Objective of this study is to review briefly exposure characteristics, monitoring instruments and threshold limit values for extremely low frequency-magnetic field (ELF-MF) methods. This study was undertaken through brief literature review. We performed a literature search in PubMed to identify ELF-MF studies conducted in workplaces. Initial search keywords such as 'extremely low frequency-magnetic field (ELF-MF)' and 'electromagnetic fields (EMF)' combined or singly. We limited our review to occupational rather than general nonworkplace environmental exposures. Methods: The contents we reviewed: key industry and occupations generating ELF-MF, several direct-reading instruments monitoring ELF-MF and threshold limit values (TLV) preventing health effects may be caused by the exposure to ELF-MF. Results: The industries related to the generation and supply of electricity, electrolytic installations, welding, and induction heating and more were regarded as high ELF-MF exposure industries. All jobs handling or employed performed in power cable lines, electrical wiring, and electrical equipment are found to be exposed to ELF-MF. Threshold or ceiling limit, 1,000 µT, is established to prevent acute effects of exposure to low-frequency EMFs on the nervous system: the direct stimulation of nerve and muscle tissues and the induction of retinal phosphenes. The International Agency for Research on Cancer (IARC) has classified ELF-MF as possibly carcinogenic to humans chiefly based on epidemiological studies on childhood leukemia. However, a causal relationship between magnetic fields and several types of cancer including childhood leukemia has not been established nor has any other long-term effects. Risk management using precautionary measures, has been initiated by the US and EU to prevent chronic health effects related to ELF-MF exposure in workplaces. Conclusion: This study recommends the implementation of various measures such as theestablishment of occupational exposure limit values for ELF-MF and precautionary principle to prevent potential chronic occupational health effects may be caused by ELF-MF in Korea.

An Energy-efficient Data Dissemination Protocol in Wireless Sensor Networks (무선 센서 네트워크에서 에너지 효율적인 데이타 전달 프로토콜)

  • Yi, Seung-Hee;Lee, Sung-Ryoul;Kim, Chong-Kwon
    • Journal of KIISE:Information Networking
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    • v.33 no.2
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    • pp.165-174
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    • 2006
  • Data dissemination using either flooding or legacy ad-hoc routing protocol is not realistic approach in the wireless sensor networks, which are composed of sensor nodes with very weak computing power, small memory and limited battery. In this paper, we propose the ELF(Energy-efficient Localized Flooding) protocol. The ELF is energy-efficient data dissemination protocol for wireless sensor networks. In the ELF protocol, there are two data delivery phases between fixed source and mobile sink node. The first phase, before the tracking zone, sensing data are forwarded by unicasting. After that, within the tracking zone, sensing data are delivered by localized flooding. Namely, the ELF Properly combines advantages from both unicasting and flooding. According to evaluation results by simulation, the proposed ELF protocol maintains very high data delivery ratio with using a little energy. Also, the property of average delay is better than others. From our research results, the ELF is very effective data dissemination protocol for wireless sensor networks.

Health Status Evaluation Based on Questionnaire SF-36 Among Residents Exposed and Non-exposed to Extremely Low Frequency-electric and Magnetic Fields (ELF-EMFs) (설문조사에 근거한 전자파 발생원 주변 주민과 비주변 주민의 건강수준 평가)

  • Jeon, Hyung Jin;Kim, Yoon Shin;Yoon, Jeong Ho;Cho, Nam Wook;Son, Seoung Woo
    • Journal of Environmental Health Sciences
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    • v.40 no.5
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    • pp.376-384
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    • 2014
  • Objective: The purpose of this study is to compare the health status of residents who were exposed to sources of extremely low frequency electromagnetic fields (ELF-EMFs) in the living environment and those who were not exposed to such a source of ELF-EMFs through a survey designed to appraise the residents' health levels. Method: Aimed at 251 residents exposed to a source of ELF-EMFs and 278 residents not exposed to a source, the survey was conducted to investigate the health status of the residents on the basis of questionnaire SF-36. Result: The health status scores of residents exposed to ELF-EMFs turned out to be lower than those of their counterparts. In terms of self-reported symptoms related to health and sleep quality, residents exposed to ELF-EMFs were found to have a higher complaint rate of self-reported symptoms such as dizziness, depression, and fatigue. Regarding sleep disorders, residents exposed to ELF-EMFs appeared to have a slightly higher complaint rate of sleep disorders compared to non-exposed residents. Conclusion: If a region is to be exposed to a source of ELF-EMFs, a more proactive policy for reducing such exposure is advised. In addition, it is necessary to better understand problems of residents by emplacing an ELF-EMFs risk information delivery system and building an efficient communication system for voluntary participation during the construction phase.

A Recovery Method of External Symbol Information in Statically-Linked ELF Files (정적 링크된 ELF 파일에서의 외부 심볼 정보 복구 기법)

  • Kim, Jung-In
    • Journal of Korea Multimedia Society
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    • v.13 no.2
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    • pp.161-170
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    • 2010
  • ELF, an abbreviation for Executable and Linkable Format, is the basic file format for shared libraries and executable files used in the Linux system, whereas 'Linker' copies the symbol information of static shared libraries into the symbol table in the target file generated by way of static linking. At this time, the symbol table keeps various pieces of debugging-related information including function names provided by the shared libraries, and it can be deleted to avoid debugging for security reasons by utilizing the fact that it does not directly affect the program execution. This paper proposes a method for restoring the symbol information of static shared libraries from the ELF object file in which the symbol table is deleted, and confirms that the symbol information is restored by conducting practical experiments.

Effect of a 60Hz electromagnetic field on the frequency of bleomycin-induced HPRT gene mutation and 1,2,4-benzenetriol-induced sister chromatid exchanges in CHO cell

  • Chung, Hai-Won;Kang, Su-Jin;Lee, Young-Joon;Kim, Su-Young
    • Journal of Radiation Protection and Research
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    • v.27 no.2
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    • pp.81-87
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    • 2002
  • The interaction of low density extremely low frequency magnetic field (ELF MF) in the frequency of hypoxanthine-guanine phosphoribosyltransferase (HPRT) mutation induced by bleomycin and on the frequency of sister chromatid exchanges (SCEs) induced by 1,2,4-benzenetriol(BT) was demonstrated. CHO cells pretreated with bleomycin or 1,2,4-benzenetriol were exposed for 24hrs to a sinusoidal 0.8mT magnetic field at 60Hz. Frequency of HPRT mutation and SCEs were determined. ELF MF exposure led to a two-fold increase of the frequency of HPRT mutation induced by bleomycin. No increase of mutation frequency was observed by ELF MF alone ELF MF also increased the frequency of SCEs induced by BT while no Increase of SCE frequencies were observed by ELF MF alone. These results suggest that low density ELF MF field would art as an enhancer rather than as an initiator of mutagenic effects in CHO cell.

Influence of Exposure to Extremely Low Frequency Magnetic Field on Neuroendocrine Cells and Hormones in Stomach of Rats

  • Hong, Min-Eui;Yoon, Kyu-Hyun;Jung, Yoon-Yang;Lee, Tae-Jin;Park, Eon-Sub;Sohn, Uy-Dong;Jeong, Ji-Hoon
    • The Korean Journal of Physiology and Pharmacology
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    • v.15 no.3
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    • pp.137-142
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    • 2011
  • Extremely low frequency magnetic fields (ELF-MF) have the ability to produce a variety of behavioral and physiological changes in animals. The stomach, as the most sensitive part of the neuroendocrine organ of the gastrointestinal tract, is crucial for the initiation of a full stress response against all harmful stress. Thus, the purpose of this study was to examine whether ELF-MF stimuli induce changes in the activity of neuroendocrine cells, considering their involvement in endocrine or paracrine effect on surrounding cells. The exposure to ELF-MF (durations of 24 h and 1 or 2 weeks, 60 Hz frequency, 0.1 mT intensity) altered the distribution and occurrence of gastrin, ghrelin and somatostatinpositive endocrine cells in the stomach of rats. The change, however, in the secretion of those hormones into blood from endocrine cells did not appear significantly with ELF-MF exposure. Comparing with sham control, ELF-MF exposure for 1 and 2 week induced an increase in $BaSO_4$ suspension propelling ratio of gastrointestinal tract, indicating that ELF-MF affects gastrointestinal motility. Our study revealed that ELF-MF exposure might influence the activity of endocrine cells, an important element of the intrinsic regulatory system in the digestive tract. The pathophysiological character of these changes and the mechanism responsible for neuroendocrine cell are still unclear and require further studies.