• IMMUNE NETWORK
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• 제2권4호
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• pp.217-222
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• 2002

Background: Scid.adh is a recently developed murine thymic lymphoma cell line, which has been used as in vitro model for the study of double negative stage III thymocytes. In this study, we compared the expression profile of a number of genes and proteins, which are tightly related to T cell development and apoptosis, in thymic lymphoma cell lines, R1.1, EL-4, and Scid.adh for the developmental staging. Methods: We examined the expression of development marker genes and proteins in three lymphoma cell lines by flow cytometry and RT-PCR. In addition, the expression of apoptosis-related molecules including bcl-2, bax and Fas was also investigated. Results: As previously reported, Scid.adh cell line expressed CD8 and CD25 but not TCR ${\alpha}$ chain, while R1.1 cells expressed TCR ${\alpha}$ chain and both CD4 and CD8 transcripts. These suggest that R1.1 might be in double positive stage, and low level of CD44 expression and the absence of CD25 support this suggestion. In contrast, EL-4 cells showed high level of TCR ${\alpha}$ chain transcript, and low-level of CD4 expression, suggesting that EL-4 is in more mature stage than R1.1. Further, this suggestion was supported by the lack of mT-20 in EL-4 cells, which is expressed in the immature thymocytes, and Scid.adh and R1.1 cell lines, but not in the terminally differentiated thymocytes and peripheral T cells. Among the apoptosis-related gene, transcripts of bcl-2 gene were detected in both R1.1 and EL-4 but not in Scid.adh cells, while bax was expressed in all cell lines. Fas expression was the highest in EL-4 cells and low in Scid.adh cell line. Conclusion: R1.1 cell may represent double positive stage, and EL-4 is more differentiated cell line. In addition, Scid.adh and EL-4 cell lines are suspected to be useful for the study of function of bcl-2 family and Fas during the thymocyte development, respectively.

• 미생물학회지
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• 제35권4호
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• pp.270-276
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• 1999

환경오염원으로서 페녹시계 제초제인 ,2,4-D(2,4-dichlorophenoxyacetic acid)에 노출된 토양으로부터 분리된 세균인 Burkholderia cepacia YK-2에서 2,4-D에 의한 스트레스 충격 단백질의 생성을 조사하였다. 활발하게 생장을 하고 있는 B. cepacia YK-2의 배양은 다양한 농도의 2,4-D에 노출되어 스트레스 충격단백질을 생성하였다. 이 반응은 43kDa의 DnaK와 41kDa의 GroEL 단백질의 생성을 수반하였으며, 이 단백질들은 anti-DnaK 단일 항체와 anti-GroEL 단일 항체를 사용한 SDS-PAGE과 Western blot을 통하여 확인되었다. 생성된 총 스트레스 충격 단백질은 2-D PAGE 에 의하여 분석되었다. 다양한 2,4-D농도와 노출 시간에 따른 B. cepacia YK-2의 생존율을 분석한 결과, 이 세균의 생존율은 스트레스 충격 단백질의 생성과 비례하였다.

• 대한화학회지
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• 제52권3호
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• pp.328-337
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• 2008

• Archives of Pharmacal Research
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• 제9권1호
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• pp.25-28
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• 1986

Twenty nine substituted thiaxanthone derivatives were synthesized for schistosomicidal evaluation. The compounds, 1-morpholino-4-methyl-6-nitrothiaxanthone and 1-(2-diethyl-aminoethyl-amino)-4 methyl-6-nitrothiaxanthone dipicrate were found to possess promising schistosomicidal activity against Schistosoma mansoni in mice.

• Biomolecules & Therapeutics
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• 제16권1호
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• pp.40-45
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• 2008

The objective of this study was to investigate the single dose and 2-week repeated dose toxicity of Aceporol 330 in ICR mice following single intravenous administration and to compare its toxicity with a commercially available solubilizer of paclitaxel, Cremophor EL. In single dose toxicity test, $LD_{50}$ of Aceporol 330 in mice was estimated to be greater than maximum applicable dose, 4 ml/kg. However, $LD_{50}$ of Cremophor EL in male mice was determined to be 4 ml/kg. Maximum tolerated dose (MTD) of males and females in Aceporol 330-treated group and MTD of females in Cremophor EL-treated group were 3 ml/kg. MTD of males in Cremophor EL-treated group was less than 3 ml/kg. Characteristic toxic symptoms, and hematological and blood chemical changes were not observed after single dose and repeated dose of Aceporol 330 or Cremophor EL. No histopathological abnormalities were found in organs of all animal groups. Based on the linear pharmacokinetic property of paclitaxel and the higher $LD_{50}$ in mice, Aceporol 330 has a potential for use as a safer solubilizer for paclitaxel than Cremophor EL.

• Mycobiology
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• 제32권1호
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• pp.35-41
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• 2004

A total of 374 Pythium isolates were isolated and identified from the rhizosphere soils of maize plants grown in 100 different agricultural fields in nine provinces at El-Minia Governorate, Egypt. Five Pythium spp. of P. deliense, P. graminicola, P. irregulare, P. oligandrum and P. splendens were obtained P. deliense and P. oligandrum were predominant in all of the locations with 48.1% and 41.4% of total counts, respectively. P. graminicola, P. irregulare and P. splendens were not isolated in 4, 2 and 4 provinces out of 9 provinces with 5.3%, 3.5%, and 1.6% of isolation percentage from total counts, respectively. Number of Pythium isolates in each were 34, 31, 34, 33, 34, 96, 37, 37 and 38 out of 374, for locations of El-Edwa, Maghagha, Beni-Mazar, Matai, Samalout, El-Minia city, Abou-Querquas, Mallawi and Der Mawas, respectively. Pre- and post-emergence pathogenicity tests indicated that only P. deliense was highly pathogenic to germinating grains and seedlings of maize whereas P. oligandrum was non-pathogenic.

• 대한화학회지
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• 제56권2호
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• pp.264-273
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• 2012

Five derivatives of phenylthiourea namely: 1-(4-methoxyphenyl)-3-phenylthiourea (1), 1-(4-methylphenyl)-3- phenylthiourea (2), 1-(4-bromophenyl)-3-phenylthiourea (3), 1-(4-chlorophenyl)-3-phenylthiourea (4) and 1-phenylthiourea (5) have been evaluated as new inhibitors for the corrosion of carbon steel in 2 M HCl solution using potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) techniques. Potentiodynamic polarization measurements showed that these derivatives are mixed-type inhibitors. The inhibition efficiency was found to increase with inhibitor concentration and decreases with rise in temperature. The thermodynamic parameters of adsorption and activation were determined and discussed. Nyquist plots showed depressed semicircles with their centre below real axis. The adsorption process of studied derivatives on carbon steel surface obeys Temkin adsorption isotherm. The synergistic effect of these derivatives and some anions is discussed from the viewpoint of adsorption models. The electrochemical results are in good agreement with the calculated quantum chemical HOMO and LUMO energies of the tested molecules.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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• pp.351-354
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• 2006

We developed highly efficient fluorescent dopants for full-color OLEDs. For blue, green and red OLEDs, current efficiencies of 8.7cd/A, 20.5 cd/A and 11.4 cd/A at $10mA/cm^2$ were achieved, respectively. Lifetime of the blue device was estimated to be 23,000hours at an initial luminance of $1,000cd/m^2$. Moreover, long lifetime over 100,000 hours was estimated in the green and red devices. Furthermore, we obtained a three-component white OLED by using these new fluorescent materials. This white OLED shows a current efficiency of 16.1cd/A with extrapolated lifetime over 70,000 hours at $1,000cd/m^2$, and more excellent color reproducibility for full-color displays with color filters and general lighting, compared to previous fluorescent white OLEDs.

• KSBB Journal
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• 제14권4호
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• pp.429-433
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• 1999

$\beta$-Glucosidaes from Cellvibrio gilvus(CG) was successfully overproduced in soluble form in E. coli with the coexpression of GroEL/ES/. Without the GroEL/ES protein, the $\beta$-glucosidase overexpressed in E. coli constituted a huge amount(80%) of total cellular protein, but was localized in the insoluble fraction, and little activity was detected in the soluble fraction. Coexpression of the E. coli GroEL/ES had a drastic impact on the proper folding of the $\beta$-glucosidase; 20% of the overexpressed enzyme was recovered in the soluble fraction in active form. Similar effects of GroEL/ES were also observed on the overexpressed $\beta$-glucosidase from Agrobacterium tumefaciens(AT). And pET28(a)-RGRAR, partially deleted mutant lacking 5-amino acid residues at carboxy teminus also could be folded into an active form when expressed with the molecular chaperonin GroEL/ES, and its activity was higher than that of the without GroEL/ES system, In addition, the synergistic effect of GroEL/ES and the low induction temperature were important factors for solubilization of the inclusion body from overproduced $\beta$-glucosidases.

• Asian Pacific Journal of Cancer Prevention
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• 제17권12호
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• pp.5281-5285
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• 2016

Background: Brachytherapy is the most commonly used conservative treatment for the uveal melanoma. The aim of this study was to evaluate therapeutic results of Ruthenium-106 plaque brachytherapy in the management of localized uveal melanoma cases. Methods: We reviewed retrospectively the clinical records of all patients treated in our department for an uveal melanoma, undergoing Ruthenium-106 plaque brachytherapy, from January 1996 to December 2015. We focused on clinical features, therapeutic characteristics, local and distant tumor control and side effects. Results: Nineteen patients were enrolled in our study. Mean age was 56.2 years (28-79) and the sex ratio was 1.37:1 males to females. Diagnosis was made on the basis of ophthalmological clinical examination, angiography, ultrasound and/or magnetic resonance. Median tumor diameter was 9.7 mm (6-13) and median thickness 4.4 mm (2.5-8). The dose of Ruthenium-106 plaque brachytherapy prescribed to the apex of each tumor was 70 Gy in all cases. The median radiation dose to the sclera surface was 226.4 Gy (range: 179.6-342.3) and the median total application time 115.2 hours (range: 27 to 237). After a median follow-up of 61.5 months, local control was achieved in 17 patients (89%): 16 demonstrated a partial tumor response and 1 tumor stabilization. Two patients suffered local progression leading to enucleation, one dying of hepatic metastasis. Radiation-induced complications were cataracts in 3 cases and vitreal hemorrhage in 2. Conclusion: Ruthenium-106 plaque brachytherapy is an efficient treatment for localized uveal melanoma, offering good local control with low toxicity.