• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2017년도 학술발표회
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• pp.249-249
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• 2017

본 연구에서는 낙동강 본류의 진동과 적포교, 남강의 거룡강, 대산, 정암 수위표의 관측된 2015년부터 2016년 2년간 수위자료를 이용하여, Self-Organizing Maps(SOM)과 LOcally WEighted Scatter plot Smoother(Lowess) 기법으로 패턴과 추세를 분석하였다. SOM 분석 결과, 낙동강 본 류의 진동과 적포교, 남강의 거룡강, 대산은 동일한 패턴과 추세를 나타냈다. 수위의 범위도, SOM 분석에서 진동 최소 EL. 4.41m, 최대 EL. 5.01m 범위, 적포교 최소 EL. 4.56m, 최대 EL. 5.38m 범위, 거룡강 최소 EL. 4.53m, 최대 EL. 5.18m 범위, 대산 최소 EL. 4.57m, 최대 EL. 5.35m 범위로 큰 차이가 발생하지 않았다. 거룡강과 대산 수위 관측지점은 낙동강 본류의 배수위 영향을 받는 것을 알 수 있었으며, 두 지점의 수위 관측 목적에 따라 상류로 지점 변경이 필요 할 수도 있을 것으로 판단된다.

• 한국응용곤충학회지
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• 제15권2호
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• pp.57-60
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• 1976

새로운 침투성 살균제 EL-291 (5-Methyl-1,2,4-tri-azolo(3, 4-b) benzothiazole, $75\%$ 수화제)의 벼 도열병 방제효과를 구명코저 온실검정과 포장시험을 실시하였다. EL-291은 병원균 접종후 살포보다 접종전 살포시 효과가 뚜렷하여 현저한 예방효과를 나타냈으나 가스가민, 벤레이트 상반된 반응을 나타내어 치료제로서 작용했다. 잎 도열병방제효과는 EL-291과 가스가민, 벤레이트간에 차이가 없었으나 이들 약제의 2회 살포에 비해 EL-291은 근침처리 또는 1회 살포한 것이므로 사용이 간편하고 경제적이라 할 수 있겠다. 이삭도열병에서도 1회 살포한 EL-291이 2회 살포한 가스가민, 벤레이트보다 월등히 효과가 좋았으며 수량도 EL-291 처리구가 더 많았다.

• 한국축산식품학회지
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• 제25권2호
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• pp.244-249
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• 2005

To investigate the effect of Holstein colostrum peptide fraction on proliferation of immune cell, polypeptide fractions were separated from acid whey into 3 fractions depending on molecular weight by ultrafiltration: Fraction I, which contains the polypeptide larger than 10,000 Da, Fraction n, which contains the polypeptide ranging from 1,000 Da to 10,000 Da and Fraction III, which contains the polypeptide smaller than 1,000 Da. EL-4 cell (murine T lymphoma cell) was used to evaluate immune enhancing effect of each fraction from Holstein colostrum. Fraction n showed the highest proliferative effect of the colostrum whey fractions on EL-4 cell at 1mg/mL compared with whole whey and other fractions and this proliferative activity was shown in dose dependent manner. Fraction n showed the highest proliferative effect on PMA (Phorbol 12-myristate 13-acetate) stimulated EL-4 cell. Heated Fraction n showed similar effect to native one on proliferation of both EL-4 cell and PMA stimulated EL-4 cell.

• International Journal of Oral Biology
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• 제37권3호
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• pp.130-136
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• 2012

The GroEL heat-shock protein from Fusobacterium nucleatum, a periodontopathogen, activates risk factors for atherosclerosis in human microvascular endothelial cells (HMEC-1) and ApoE-/- mice. In this study, we analyzed the signaling pathways by which F. nucleatum GroEL induces the proinflammatory factors in HMEC-1 cells known to be risk factors associated with the development of atherosclerosis and identified the cellular receptor used by GroEL. The MAPK and NF-${\kappa}B$ signaling pathways were found to be activated by GroEL to induce the expression of interleukin-8 (IL-8), monocyte chemoattractant protein 1 (MCP-1), intercellular adhesion molecule 1 (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), E-selectin, and tissue factor (TF). These effects were inhibited by a TLR4 knockdown. Our results thus indicate that TLR4 is a key receptor that mediates the interaction of F. nucleatum GroEL with HMEC-1 cells and subsequently induces an inflammatory response via the MAPK and NF-${\kappa}B$ pathways.

• Archives of Pharmacal Research
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• 제17권3호
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• pp.194-198
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• 1994

The antitumor effects of the 2E4 and anti--Tac, monoclonal antibodies directed to Il-2 receptor (IL-2R) conjugated with .alpha.-particle emitting radionuclide bismuth-212., were compared. The $^{212}Bi-2E4$ demonstratedspecific cytotocicity to EL4J3, 4, a I$L-2R^+$ cell line, than to EL4J, a $IL-2R^-$ cell line in thymidine incorporation assy. TEX>$^{212}Bi-2E4$ exerted the maximal antitumor effect in that % T/C in C57BL/6 mice implanted with EL4J3.4 ascitic tumor was 331% at the concentration of $50{\;}{\mu}Ci$, while that of $^{212}Bi-anti-Tac$ was 258% at $100{\;}{\mu}Ci$.

• 대한수의학회지
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• 제29권4호
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• pp.469-474
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• 1989

The changes of plasma prostaglandin $E_2$ level, natural killer cell activity and tumor cell growth were assayed after transplantation of EL-4 leukemia cells in C57BL/6 mice. The results were summarized as follows; 1. Plasma prostaglandin $E_2$ level was increased in EL-4 bearing mice, but indomethacin treated mice group showed low level. 2. The tumor-derived prostaglandin $E_2$ inhibited the post-target binding cytolytic process of natural killer activity. 3. Indomethacin inhibited the growth of prostaglandin secreting EL-4 solid tumor.

• Radiation Oncology Journal
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• 제8권2호
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• pp.137-144
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• 1990

Filter elution 방법으로 EL 4백서 백혈병 세포 및 C57BL/6백서 유래의 비장 임파구에 대한 Co-60 $\gamma$ 선의 DNA single-strand breaks (SSB) 효과를 정량적으로 측정하였다. 임파구는 [${^3}H$]thymidine을 표지하기 위하여lipopolysaccharide (LPS, 20 $\mug/ml$)를 첨가하여 자극하고 부유 상태의 EL 4 세포 및 임파구를 $0^{\circ}C$에서 0 Gy, 1 Gy, 5 Gy, 10 Gy 또는 15 Gy조사하였으며, elution용액의 pH는 12.1로 하였다. $\gamma$ 선 조사에 따른 single-strand breaks의 수는 방사선 조사량에 따라 증가 하였으며 21 ml elution 양을 기준으로 한 strand scission factor (SSF)는 EL 4 세포에서 $0.01301\pm0.00096\;Gy^{-1}(n=5)$이었고, 임파구는 $0.01097\pm0.00091\;Gy^{-1}(n=5)$를 나타내므로 본 실험에서는 EL 4 세포가 정상 임파구에 비하여 방사선에 의한 DNA SSB가 민감함을 알 수 있었다(p<0.005). 본 연구 결과 DNA strand breaks의 측정법을 이용하여 방사선의 특성 및 생물학적 효과의 파악은 물론 나아가 기존의 방호제 및 새로운 약제의 DNA에 대한 효과를 판별할 수 있을 것이다.

• 대기
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• 제22권4호
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• pp.465-472
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• 2012

Using various observed data, we examined the evolution of tropical Pacific sea surface temperature (SST) during 2011-2012, with focusing on the development of 2012 El Ni$\tilde{n}$o. It is observed that a La Ni$\tilde{n}$a event during 2011 was followed by a moderate El Ni$\tilde{n}$o during 2012 summer. The 2012 summer El Ni$\tilde{n}$o initiated near the west coast of South America on February 2012, and continued to expand westward till August. Given this evolutionary pattern, the 2012 summer El Ni$\tilde{n}$o can be categorized as 'Eastern Pacific (EP) El Ni$\tilde{n}$o' because Ni$\tilde{n}$o-3 index is greater than Ni$\tilde{n}$o-4 index, and it may be the first well-defined EP El Ni$\tilde{n}$o since 2001. On February 2012, this event was initiated mainly by the local air-sea interaction, and at the same time the ocean heat content was accumulated over the tropical western Pacific due to the easterly wind anomaly over the tropical western Pacific. Then, the accumulated heat content slowly propagates to the tropical eastern Pacific, which attributes to maintain El Ni$\tilde{n}$o state during 2012 summer. After August, the positive SST anomaly over the equatorial eastern Pacific decays possibly due to the exhausted heat content and the weakening of air-sea interaction, but the weak positive SST anomaly over the central Pacific remains till now (2012 November).

• 한국미생물·생명공학회지
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• 제24권3호
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• pp.344-351
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• 1996

Microorganisms growing alcoholic distillery wastes were isolated from soil. Of them, the selected strain EL-9 had a capability of accumulating poly-$\beta$-hydroxybutyrate (PHB) when grown in alcoholic distillery wastes. The strain EL-9 was identified as a genus Actinobacillus based on the morphological, cultural, and biochemical characteristics. The strain EL-9 was named temporarily as Actino- bacillus sp. EL-9. The optimal temperature and pH for cell growth of Actinobacillus sp. EL-9 were 30$\circ$C and 7.0, respectively. The optimal medium compositions for cell growth comprised glucose 2%, NH$_{4}$NO$_{3}$ 0.15%, Na$_{2}$HP0$_{4}$-12H$_{2}$O 0.25%, KH$_{2}$PO$_{4}$ 0.25%, MgSO$_{4}$4-7H$_{2}$O 0.15%, FeSO$_{4}$-7H$_{2}$O 0.005%, CaCl$_{2}$-2H$_{2}$O 0.003% and trace element solution 5m/l. To investigate the optimal conditions for PHB production, two-stage culture technique was used. The result showed that the optimal conditions for PHB production are identical those for cell growth. When propionic acid was added as a cosubstrate, PHB/HV copolymer was produced.

• 대한수의학회지
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• 제63권1호
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• pp.2.1-2.7
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• 2023

Fenbendazole (FBZ) is a benzimidazole anthelmintic widely used to treat parasitic infections. The anticancer effect of FBZ has been recently highlighted leading to its consideration as a potential anticancer agent. Although previous studies have demonstrated the effect of FBZ on cancer cells, there is a paucity of studies on the effect of FBZ on lymphoma cells and normal immune cells. Herein, we investigated the effects of FBZ on a mouse lymphoma cell line, EL-4 cells, and spleen cells, using vincristine as a positive control. The cellular metabolic activity of EL-4 cells was decreased by FBZ, but that of the spleen cells was not decreased. Moreover, FBZ reduced the mitochondrial membrane potential and induced reactive oxygen species production in EL-4 cells, but not in spleen cells. FBZ induced G2/M phase arrest and increased the sub G0/G1 phase ratio, indicating apoptosis. Furthermore, compared to the control cells, the reactivity of spleen cells pretreated with FBZ to lipopolysaccharide was maintained. In summary, FBZ is cytotoxic to EL-4 cells, but not to spleen cells. This study provides experimental evidence that FBZ exerts an anticancer effect, and less cytotoxic effects and functional damage to normal spleen cells.