• Title/Summary/Keyword: EHD

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Molecular Authentication of Schisandrae Fructus and Analysis of Phylogenetic Relationship based on nrDNA-ITS sequences (nrDNA-ITS 분자마커를 이용한 오미자(五味子) 종 감별 및 기원분석 -ITS 염기서열을 이용한 오미자(五味子) 감별-)

  • Moon, Byeong-Cheol;Ji, Yun-Ui;Seo, Hyeong-Seok;Lee, A-Young;Chun, Jin-Mi;Kim, Ho-Kyoung
    • The Korea Journal of Herbology
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    • v.25 no.4
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    • pp.47-54
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    • 2010
  • Objectives : The original plant species of Schisandrae Fructus (O-mi-ja) is prescribed as Schisandra chinensis $B_{AILL.}$, in Korea, but S. chinensis $B_{AILL.}$ and S. sphenanthera $R_{EHD.}$ et $W_{ILS.}$ in China. Moreover, fruit of several other species in genus Schisandra also have been used as the same herbal medicines. To develop a reliable method for correct identification of Schisandrae Fructus and to evaluate the phylogenetic relationship of S. chinensis and its related species, we analyzed internal transcribed spacer (ITS) sequences of nuclear ribosomal DNA (nrDNA). Methods : Twenty-four plant samples of three Schisandra species and one Kadsura species, S. chinensis $B_{AILL.}$, S. spenanthera $R_{EHD.}$ et $W_{ILS.}$, S. nigra $M_{ax.}$ and Kadsura japonica $D_{UNAL}$ were collected from each different native habitate and farm in Korea and China. The nrDNA-ITS region of each samples were amplified using ITS1 and ITS4 primer and nucleotide sequences were determined after sub-cloning into the pGEM-Teasy vector. Authentic marker nucleotides were estimated by the analysis of ClastalW based on the entire nrDNA-ITS sequence. Results : In comparative analysis of the nrDNA-ITS sequences, we found specific nucleotide sequences including indels (insertions and deletions) and substitutions to distinguish C. chinensis, S. spenanthera, S. nigra, and K. japonica. These sequence differences at corresponding positions are avaliable nucleotide markers to determine the botanical origin of O-mi-ja. Moreover, we evaluated the phylogenetic relationship of four plant species by the analysis of nrDNA-ITS sequences. Conclusions : These marker nucleotides would be useful to identify the official herbal medicines by the providing of definitive information that can identify each plant species and distinguish it from unauthentic adulterants for O-mi-ja.

Image Retrieval Using Multiresoluton Color and Texture Features in Wavelet Transform Domain (웨이브릿 변환 영역의 칼라 및 질감 특징을 이용한 영상검색)

  • Chun Young-Deok;Sung Joong-Ki;Kim Nam-Chul
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.43 no.1 s.307
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    • pp.55-66
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    • 2006
  • We propose a progressive image retrieval method based on an efficient combination of multiresolution color and torture features in wavelet transform domain. As a color feature, color autocorrelogram of the hue and saturation components is chosen. As texture features, BDIP and BVLC moments of the value component are chosen. For the selected features, we obtain multiresolution feature vectors which are extracted from all decomposition levels in wavelet domain. The multiresolution feature vectors of the color and texture features are efficiently combined by the normalization depending on their dimensions and standard deviation vector, respectively, vector components of the features are efficiently quantized in consideration of their storage space, and computational complexity in similarity computation is reduced by using progressive retrieval strategy. Experimental results show that the proposed method yields average $15\%$ better performance in precision vs. recall and average 0.2 in ANMRR than the methods using color histogram color autocorrelogram SCD, CSD, wavelet moments, EHD, BDIP and BVLC moments, and combination of color histogram and wavelet moments, respectively. Specially, the proposed method shows an excellent performance over the other methods in image DBs contained images of various resolutions.

Evaluation of the Characteristics of High-Flux Reverse Osmosis Membranes with Various Additives (다양한 첨가제에 따른 고투과성 역삼투막의 특성평가)

  • Hyun Woong Kwon;Kwang Seop Im;Gede Herry Arum Wijaya;Seong Min Han;Seong Heon Kim;Jun Ho Park;Dong Jun Lee;Sang Min Eom;Sang Yong Nam
    • Membrane Journal
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    • v.33 no.6
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    • pp.427-438
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    • 2023
  • In this study, in order to improve the performance of the reverse osmosis membrane with high water flux and high salt rejection, a study was conducted on the evaluation of characteristics according to the curing temperature and time during various additives and interfacial polymerization. The morphology of the membrane with no additives and the membrane with additives both showed a "rigid-and-valley" structure, confirming that the polyamide layer was successfully polymerized on the surface of the porous support layer. In addition, the additive of 2-Ethyl-1,3-hexanediol (EHD) had improved hydrophilicity and water flux, which was confirmed by measuring the contact angle. Finally, a highly permeable TFC membrane with NaCl and MgSO4 salt rejection of 97.78% and 98.7% and a high water flux of 3.31 L/(m2⋅h⋅bar) was prepared.

High Speed and Continuous Electrospinning Printing Using Polymer Ink (고분자 폴리머 잉크를 이용한 고속 연속 전기 방사 프린팅)

  • Zhang, Da-Hai;Kwon, Kye-Si
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.39 no.4
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    • pp.379-384
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    • 2015
  • Electrospinning has recently been used for micropatterning. The electrospinning method as a patterning tool has the advantage of a rapid patterning speed because it is based on a continuous printing mode rather than a drop-on-demand mode. To obtain stable continuous printing, a high molecular weight polymer must be mixed with functional materials for patterning. In this paper, polyethylene oxide (PEO) was used. The effect of polymer on viscosity and formation of a Taylor cone jet from the electrospinning nozzle was investigated. Finally, the electrospinning patterning results of a silver paste ink on a glass substrate were investigated.

An experimental study for boiling heat transfer enhancement under electric fields (전기장하에서의 비등 열전달 촉진에 관한 실험적 연구)

  • O, Si-Deok;Gwak, Ho-Yeong
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.20 no.7
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    • pp.2298-2314
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    • 1996
  • Electric field effect on boiling of refrigerants R11, R113, and FC72 has been investigated experimentally. One purpose of the experimental investigation is to determine the effects of the electrode arrangements on electrohydrodynamic boiling of the above mentioned liquids. The test equipment employed in the experiment consists of a shell and tube heat exchanger with six or six and twelve rows of electrode wires around the tube. It has been found that the applied voltage promotes the boiling heat transfer coefficient except FC72. Boiling heat transfer enhancement obtained is about 230% for R11, 280% for R113. It has also been observed that bubbles detached from the tube aggregate at the place where the electrical gradient force balances with the buoyancy one. These aggregated bubbles force to decrease the boiling heat transfer coefficient as well as to reduce the voltage needed to the dielectric breakdown.

The impact behaviors of electrified micro-droplet with existence and nonexistence of electrical charged for surface (표면 전하 유무에 따른 대전된 미소액적의 충돌 현상)

  • Lee, Jaehyun;Kim, Jihoon;Byun, Doyoung
    • Journal of the Korean Society of Visualization
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    • v.13 no.1
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    • pp.49-53
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    • 2015
  • Recently, researches for droplet impact phenomena have been faced a new phase in the direction of studying the effect of complex external conditions (e.g. wettability, temperature, morphology, electric field, etc.) for depth understanding and precise controlling in various applications. Hence, here we investigated the electrified droplet impact phenomena, because there were few quantitative researches for electrified droplet impact when we considering many real applications such as electrospray, electrohydrodynamic (EHD) jet printing. To observe interaction effect of surface charge between substrate and droplet simultaneously, micro-droplets with various Reynolds number (Re) and Weber number (We) were dripped on super-hydrophobic surface with existence and nonexistence of electrical surface charge. It shows three kinds of impact behaviors, fully bouncing, partial bouncing, and splashing with different We. Also, charged droplet bounced higher on electrically charged surface than on non-charged surface. Additionally, transition regions of three impact behaviors were classified quantitatively with water hammer pressure value, which means instant pressure inside droplet at the impact moment.

Molecular Cloning of a Cellulase Gene from Abalone Haliotis discus hannai and Its Expression in E coli

  • Park, Eun-Mi;Han, Yun-Hee;Park, In-Suk;Nam, Bo-Hye;Kong, Hee Jeong;Kim, Woo-Jin;Lee, Sang-Jun;Kim, Young-Ok
    • Journal of Marine Bioscience and Biotechnology
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    • v.2 no.2
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    • pp.108-112
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    • 2007
  • A cellulase (endo-${\beta}$-1,4-D-glucanase(E.C.3.2.1.4)) was isolated from the hepatopancreas of abalone Haliotis discus hannai by EST analysis. The abalone cellulase named HdEG compassed 1977 bp, including 195 bp in the 5'untranslated region, 1680 bp in the open reading frame which encodes 560 amino acid residues, and 92 bp in the 3'-untranslated region. The C-terminal region of the HdEG showed 44-52% identity to the catalytic domains of glycoside hydrolase family 9 (GHF9)-cellulases from arthropods and bacteria. The recombinant cellulase, pEHdEG was produced in E. coli with being fused with C-terminal His-tag. The expressed protein showed a single band (~62 kDa) on Western blotting which was consistent with the value (61,878 Da) calculated from the DNA sequence.

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Context Extraction and Analysis of Video Life Log Using Bayesian Network (베이지안 네트워크를 이용한 동영상 기반 라이프 로그의 분석 및 의미정보 추출)

  • Jung, Tae-Min;Cho, Sung-Bae
    • Proceedings of the Korean Information Science Society Conference
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    • 2010.06c
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    • pp.414-418
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    • 2010
  • 최근 라이프 로그의 수집과 관리에 관련된 연구가 많이 진행 중에 있다. 또 핸드폰 카메라, 디지털 카메라, 캠코더 등의 발전으로 자신의 일상생활을 비디오로 저장하고, 인터넷을 통해 공유하는 사람도 증가하고 있다. 비디오 데이터는 많은 정보를 포함하고 있는 라이프 로그의 한 예로. 동영상의 촬영 및 수집이 활발해짐에 따라 동영상의 메타정보를 생성하고, 이를 이용해 동영상 검색과 관리에 이용하려는 연구들이 진행 중이다. 본 논문에서는 라이프 로그를 수집하고 수집된 동영상과 라이프 로그를 이용하여 의미정보를 추출하는 시스템을 제안한다. 의미정보란 사용자의 행동을 나타내는 정보로써 컴퓨터 사용, 식사, 집안일, 이동, 외출, 독서, 휴식, 일, 기타로 9가지의 의미정보를 추출한다. 제안하는 방법은 사용자로부터 GPS, 가속도센서, 캠코더를 이용해 실제 데이터를 수집하고, 전처리 과정을 통하여 특징을 추출한다. 이때 추출될 특징은 위치정보와 사용자의 상태정보 그리고 영상처리릍 통한 RGB와 HSL 색공간의 요소와 MPEG-7의 EHD(Edge Histogram Descriptor). CLD(Color Layout Descriptor)이다. 추출된 특징으로부터 사람 행동과 같은 불안정한 상황에서 강점을 보이는 확률모델 네트워크인 베이지안 네트워크를 이용하여 의미정보를 추출한다. 제안하는 방법의 유용성을 보이기 위해 실제 데이터를 수집하고 추론하고 10-Fold Cross-validation을 이용하여 데이터를 검증한다.

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Batch and dynamic study of lactic acid extraction using emulsion liquid membrane

  • Berrama, Tarek;Pareau, Dominique;Durand, Gerard
    • Membrane and Water Treatment
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    • v.6 no.4
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    • pp.277-292
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    • 2015
  • The extraction of lactic acid by an emulsion liquid membrane (ELM), in batch and continuous mode, has been reported. On the basis of preliminary experiments, the optimum composition of the organic phase (membrane) is determined. When the SPan 80 is used as surfactant, the emulsion breakage exceeds 50%, but only 10% is obtained when the ECA4360 is used. The effects of surfactant, carrier and solute concentrations, phase volume ratio, and stirring speed on the extraction yield were examined and optimized. Surfactant, carrier and diluent used were ECA4360, trilaurylamine (TLA) and dodecane, respectively; 2-ethylhexane-1,3-diol (EHD) is used as a co-surfactant. Under optimal conditions, emulsion breaking is very low and the swelling is kept at its lowest level. Under the pH conditions of fermentation medium, the extraction yield is lower. A mixer-settler continuous system was used for testing these conditions. The residence time, the number of extraction stages and the stability of the emulsion were studied and optimized. The extraction yield obtained exceeds 90%.

Current Status of Management on Pharmacopuncture in Korea through Introduction of an Accreditation System

  • Sung, Soo-Hyun;Shin, Byung-Cheul;Park, Min-Jung;Kim, Kyeong Han;Kim, Ji-Won;Ryu, Ji-Yeon;Park, Jang-Kyung
    • Journal of Pharmacopuncture
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    • v.22 no.2
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    • pp.75-82
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    • 2019
  • Objectives: Pharmacopuncture is a new form of acupuncture treatment that injects herbal medicine into acupuncture points. This paper introduces the management status of pharmacopuncture through accreditation, and examines the effect of accreditation on pharmacopuncture management. Methods: The Accreditation System of External Herbal Dispensaries (EHDs) of traditional Korean medicine clinics announced by the Ministry of Health and Welfare in September 2018 were investigated. Results: The Accreditation System of EHDs assesses and certifies herbal medicine and pharmacopuncture preparations. Regular components for the 'pharmacopuncture' certification consist of nine standards, 30 categories, and 165 items. The nine standards include: herbal dispensary facilities, clean room management, management and organization operation, employee management, document management, continuous quality control, herbal medicine management, management of preparation, and pavement management. Conclusion: Through EHD accreditation and certification system, traditional Korean medicine clinics and EHDs can now manage pharmacopuncture medicine quality and promise safe pharmacopuncture treatment for the people.