• KSBB Journal
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• v.19 no.1
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• pp.42-49
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• 2004

A gene coding for epoxide hydrolase (EH) of Aspergillus niger LK, a fungus possessing the enantioselective hydrolysis activity for racemic epoxides, was characterized by phylogenetic analysis. The deduced protein of A. niger LK epoxide hydrolase shares significant sequence similarity with several bacterial EHs and mammalian microsomal EHs (mEH) and belongs to the a/${\beta}$ hydrolase fold family. EH from A. niger LK had 90.6% identity with 3D crystal structure of lqo7 in Protein Data Bank. Sequence comparison with other source EHs suggested that Asp$\^$l92/, Asp$\^$374/ and His$\^$374/ constituted the catalytic triad. Based on the multiple sequence comparison of the functional and structural domain sequence, the phylogenetic tree between relevant epoxide hydrolases from various species were reconstructed by using Neighbor-Joining method. Genetic distances were so far as 1.841-2.682 but characteristic oxyanion hole and catalytic triad were highly conserved, which means they have diverged from a common ancestor.

• KSBB Journal
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• v.20 no.4
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• pp.311-316
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• 2005

Epoxide hydrolases (EHs) are versatile biocatalysts for the preparation of chiral epoxides by enantioselective hydrolysis from racemic epoxides. Various microorganisms were identified to possess a EH activity by multiple sequence alignment and analysis of conserved domain sequence from genomic and megaplasmid sequence data. We successfully isolated Gordonia westfalica possessing EH activity from various microbial strains from culture type collections. G. westfalica exhibited (R)-styrene oxide preferred enantioselective hydrolysis activity. Chiral (S)-styrene oxide with high optical purity $(>\;99\%)\;ee)$ and yield of $36.5\%$ was obtained from its racemate using whole-cell of G. westfalica.

• Journal of Ocean Engineering and Technology
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• v.28 no.1
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• pp.34-46
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• 2014

This paper discusses a new formulation for the failure strain in the average stress triaxiaility domain for a low-temperature high-strength steel (EH36). The new formula available at a low average stress triaxiality zone is proposed based on the comparison of two results from tensile tests of flat type specimens and their numerical simulations. In order to confirm the validity of the failure strain formulation, a user-subroutine was developed using Abaqus/Explicit, which is known to be one of the most popular commercial finite element analysis codes. Numerical fracture simulations with the user-subroutine were conducted for all the tensile tests. A comparison of the engineering stress-strain curves and engineering failure strain obtained from the numerical simulation with the user-subroutine for the tensile tests revealed that the newly developed user-subroutine effectively predicts the initiation of failure.

• Journal of Ocean Engineering and Technology
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• v.27 no.2
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• pp.19-26
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• 2013

Stress triaxiality is recognized as one of the most important factors for predicting the failure strain of ductile metals. This study dealt with the effect of the average stress triaxiality on the failure strain of a typical low-temperature high-strength marine structural steel, EH36. Tensile tests were carried out on flat specimens with different notches, from relatively smooth to very sharp levels. Numerical simulations of each specimen were performed by using ABAQUS. The failure initiation points in numerical simulations were identified from a comparison of the engineering stress vs. strain curves obtained from experiments with simulated ones. The failure strain curves for various dimensionless critical energy levels were established in the average stress triaxiality domain and compared with the identified failure strain points. It was observed that most of the failure initiation points were approximated with a 100% dimensionless critical energy curve. It was concluded that the failure strains were well expressed as a function of the average stress triaxiality.

• Journal of Life Science
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• v.30 no.1
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• pp.10-17
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• 2020

Kinesin-1 is microtubule-dependent plus-end direct molecular motor protein essential for intracellular transport. It is a member of the kinesin superfamily proteins (KIFs) which transport cargo, including organelles, vesicles, neurotransmitter receptors, cell-signaling molecules, and protein complexes through interaction between its light chain subunit and the cargo. Kinesin light chain 1 (KLC1) is a non-motor subunit that associates with the kinesin heavy chain (KHC). Although KLC1 interacts with many different adaptor proteins and scaffolding proteins, its binding proteins have not yet been fully identified. We used the yeast two-hybrid assay to identify proteins that interact with the tetratricopeptide repeat (TPR) domain of KLC1, and found an interaction between KLC1 and EH domain-binding protein 1 like 1 (EHBP1L1). EHBP1L1 bound to the region containing all six TPR repeats of KLC1 and did not interact with KIF5B (a motor protein of kinesin 1) or KIF3A (a motor protein of kinesin 2) in the yeast two-hybrid assay. The carboxyl-terminus of the coiled-coil domain of EHBP1L1 is essential for interaction with KLC1. However, another EHBP1L1 isoform, EHBP1, did not interact with KLC1 in the yeast two-hybrid assay. KLC1 interacted with GST-EHBP1L1 and its coiled-coil domain but not with GST only. When co-expressed in HEK-293T cells, EHBP1L1 co-localized with KLC1 and co-immunoprecipitated with KLC1 and KIF5B but not KIF3A. These results suggest that kinesin 1 motor protein may transport EHBP1L1-associated cargo in cells.

• Journal of Microbiology and Biotechnology
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• v.14 no.1
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• pp.134-139
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• 2004

Hybrid cluster protein (HCP) was investigated because of its unique iron-sulfur clusters, which have been found in bacteria and archaea. Here, HCP homologous proteins from the third domain, 'eukarya'(3 amitochondriate protozoans, Giardia lamblia, Entamoeba histolytica, and Trichomonas vaginalis), were identified. All three amitochondriate protozoan HCPs (GlHCP, EhHCP, and TvHCP) belonged to Class I on the basis of two key characters, the cysteine spacing, Cys-(Xaa)₂Cys(Xaa)/sub 7-8/-Cys(Xaa)/sub 5/-Cys, and the absence of N-terminal deletion characteristic to the Class III. In phylogenetic analysis performed with amino acid sequences of 3 eukaryal, 5 bacterial, and 4 archaeal HCPs, the maximum likelihood (ML) tree indicated that TvHCP was clustered with Class I HCPs, whereas the other two HCPs (GlHCP and EhHCP) formed an independent clade with a high bootstrapping value (96%) not belonging to any previously recognized HCP class. In spite of the relatively lower bootstrapping value (61%), the position of the new eukaryal GlHCP-EhHCP clade was close to Class I, including the TvHCP, and Classes II and III were closely related with each other. The finding of eukaryal HCPs would help to understand the evolutionary history of HCP.

• The Transactions of the Korea Information Processing Society
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• v.7 no.6
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• pp.1956-1963
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• 2000

In this paper, a wavelet image coding scheme exploiting the correlation of neighboring VQ indices in eh wavelet domain is proposed. the codewords in each sub-codebook are re-ordered in terms of their energies in order to increase the correlation of he indices. Then, the generated indices after VQ can be further encoded by non-adaptive DPCM/Huffman method. LBG algorithm and a fast-PNN algorithm using k-d trees are used for generating a multiresolution codebook. Experimental results show that or scheme outperforms the ordinary wavelet VQ and JPEG at low bit rates.

• Health Policy and Management
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• v.15 no.4
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• pp.110-135
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• 2005

The objectives of this study were (1) to describe doctors' perception and attitudes toward patient safety culture and medical error reporting in their working unit and hospitals, (2) to examine whether these perception and attitudes differ by doctors' characteristics, such as sex, position, and specialties, and (3) to understand the relationship between overall perception of patient safety in their working unit and each sub domain of patient safety culture. A survey was conducted with 135 doctors working in a university hospital in Korea. After descriptive analyses and chi-square tests of subgroup differences, a multivariate-regression of overall perception of patient safety in their unit with sub-domains of patient safety culture was conducted. Overall, a significant proportion of doctors expressed negative perception of their working units' patient safety culture, many reporting potentials for patient safety problems to occur in their unit. They also negatively viewed their hospital leadership's commitment on patient safety. Regarding the patient safety in their working unit, doctors were most worried about staffing level and observance of safety procedures. Most doctors did not know how and which medical error to report. They also perceived that medical errors would work against them personally and penalize them. About 22 percent of respondents believed that even seriously harmful medical errors were not reported.

• Journal of Ocean Engineering and Technology
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• v.29 no.6
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• pp.445-453
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• 2015

The stress triaxiality and lode angle are known to be most dominant fracture parameters in ductile materials. This paper proposes a three-dimensional failure strain surface for a ductile steel, called a low-temperature high-tensile steel (EH36), using average stress triaxiality and average normalized lode parameter, along with briefly introducing their theoretical background. It is an extension of previous works by Choung et al. (2011; 2012; 2014a; 2014b) and Choung and Nam (2013), in which a two-dimensional failure strain locus was presented. A series of tests for specially designed specimens that were expected to fail in the shear mode, shear-tension mode, and compression mode was conducted to develop a three-dimensional fracture surface covering wide ranges for the two parameters. This paper discusses the test procedures for three different tests in detail. The tensile force versus stroke data are presented as the results of these tests and will be used for the verification of numerical simulations and fracture identifications in Part II.