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Bioinformatics based Identification and Characterization of Epoxide Hydrolase of Gordonia westfalica for the Production of Chiral Epoxides  

Lee Soo Jung (Department of Food Science and Technology, Kyungsung University)
Lee Eun Jung (Department of Food Science and Technology, Kyungsung University)
Kim Hee Sook (Department of Food Science and Technology, Kyungsung University)
Lee Eun Yeol (Department of Food Science and Technology, Kyungsung University)
Publication Information
KSBB Journal / v.20, no.4, 2005 , pp. 311-316 More about this Journal
Abstract
Epoxide hydrolases (EHs) are versatile biocatalysts for the preparation of chiral epoxides by enantioselective hydrolysis from racemic epoxides. Various microorganisms were identified to possess a EH activity by multiple sequence alignment and analysis of conserved domain sequence from genomic and megaplasmid sequence data. We successfully isolated Gordonia westfalica possessing EH activity from various microbial strains from culture type collections. G. westfalica exhibited (R)-styrene oxide preferred enantioselective hydrolysis activity. Chiral (S)-styrene oxide with high optical purity $(>\;99\%)\;ee)$ and yield of $36.5\%$ was obtained from its racemate using whole-cell of G. westfalica.
Keywords
Enantioselective hydrolysis; epoxide hydrolase; chiral epoixdes; Gordonia westfalica; multiple sequence alignment;
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