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Activities of Hepatocytes and Changes of Protein and Total RNA Contents in Liver and Muscle of Sebastes schlegeli with the Gonadal Maturation (조피볼락(Sebastes schlegeli)의 성숙에 따른 간세포 활성과 간 및 근육의 단백질$\cdot$총 RNA 함량변화)

  • CHUNG Ee-Yung;CHANG Young Jin;LEE Keun-Kwang
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.3
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    • pp.338-346
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    • 1995
  • Studies on activities of hepatocytes and changes of protein and total RNA contents in liver and muscle tissues associated with the gonadal maturation were performed using the adult rockfish, Sebastes schlegeli collected monthly from the adjacent waters at Poryong, Chungnam, Korea. Hepatosomatic index (HSI) of female increased from September and reached the maximum value (2.58) in February (growing stage of ovary). HSI of male began to increase from October (early maturation stage) and revealed the maximum value (3.20) in April (recovery stage). During the period of yolk formation in oocyte, the amount of glycogen particles and lipids in the hepatocytes of female gradually decreased, but total RNA contents increased. In the hepatocytes of male, a number of lipid droplets remained until the mature stage of testis. With the gonadal development of both sexes, granular endoplasmic reticula (Er) became abundant in the hepatocyte. These Ers might be supposed to have the leading role for the syntheses of protein and vitellogenin in the cytoplasm of the hepatocytes. Protein and total RNA contents in the liver and muscle tissues of female began to increase from January (growing stage) and reached the maximum value in early April (maturation stage), and then decreased in late April (gestation stage). In male, their contents began to increase from October (early maturation stage), thereafter reached the maximum value in December (maturation and copulation stage), and decreased in January (degeneration stage).

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Effect of feeding tamarind kernel powder extract residue on digestibility, nitrogen availability and ruminal fermentation in wethers

  • Wang, Lin;Nakanishi, Takashi;Sato, Yoshiaki;Oishi, Kazato;Hirooka, Hiroyuki;Takahashi, Kei;Kumagai, Hajime
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.3
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    • pp.379-385
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    • 2017
  • Objective: This study was to examine in vivo digestibility, nitrogen balance and ruminal fermentation of tamarind (Tamarind indica) kernel powder extract residue (TKPER) compared to soybean products and by-products in wethers. Methods: Four wethers with initial body weight (BW) of $51.6{\pm}5.5kg$ were assigned in a $4{\times}4$ Latin square design to investigate nutritional characteristics of TKPER, dry heat soybean (SB), dry soybean curd residue (SBCR) and soybean meal (SBM) feeding with ryegrass straw (R) at a ratio of 1:1 at 2% of BW in dry matter (DM) on a daily basis. Results: The digestibility of DM, crude protein, and ether extract (EE) of TKPER-R diet were 57.0%, 87.0%, and 86.0%, respectively. Higher non-fiber carbohydrates digestibility was observed in TKPER-R diet (83.2%) than in SB-R diet (73.9%, p<0.05). Wethers fed the TKPER-R diet had lower retention of nitrogen (N) and ruminal ammonia nitrogen ($NH_3-N$) contents at 4 h after feeding than those fed the SBM-R diet (p<0.05), which had values similar to the SB-R or SBCR-R diet. The TKPER feeding had higher propionate (C3) and lower butyrate content, as well as lower acetate to propionate ratio (C2:C3) in rumen fluid than SBM feeding at 4 h after feeding (p<0.05). Conclusion: TKPER did not bring any side effect to the wethers although it was lack of fiber, and could be used as a high protein and energy ingredient in concentrate with appropriate roughage to meet the fiber requirement for ruminants.

Oligosaccharides are a key factor in prediction of amino acid digestibility in soybean meal of different origins when fed to growing pigs

  • Li, Zhongchao;Wang, Qiuyun;Xie, Fei;Liu, Dewen;Li, Yakui;Lyu, Zhiqian;Lai, Changhua
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.12
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    • pp.1724-1732
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    • 2017
  • Objective: The objective of this experiment was to determine apparent ileal digestibility (AID) and standardized ileal digestibility (SID) of crude protein (CP) and amino acid (AA) in 15 sources of soybean meal (SBM) produced from soybeans from different countries and subsequently to establish equations for predicting the AID and SID in SBM based on their chemical composition. Methods: Eighteen barrows ($57.9{\pm}6.1kg$) fitted with a simple T-cannula were allotted into three $6{\times}6$ Latin square designs. Each period comprised a 6-d adaption period followed by a 2-d collection of ileal digesta. The 15 test diets included SBM as a sole source of AA in the diet. Another nitrogen-free diet was used to measure basal endogenous losses of CP and AA. Chromic oxide (0.3%) was used as an inert marker in each diet. Results: The AID of lysine in SBM from China and USA tended to be greater than in SBM from Brazil (p<0.10). The SID of valine and proline in SBM from China was greater than in SBM from Brazil (p<0.05). The SID of lysine, threonine, cysteine and glycine in SBM from China tended to be greater than in SBM from Brazil (p<0.10). From a stepwise regression analysis, a series of AID and SID prediction equations were generated. The best fit equations for lysine in SBM were: AID lysine = 1.16 sucrose-1.81 raffinose+82.10 ($R^2=0.69$, p<0.01) and SID lysine = 1.14 sucrose-1.93 raffinose-0.99 ether extract (EE)+85.26 ($R^2=0.77$, p<0.01). Conclusion: It was concluded that under the conditions of this experiment, the oligosaccharides (such as sucrose and raffinose) can be used to predict the AID and SID of AA in SBM with reasonable accuracy.

A Study on Drinking and Smoking Habits of the Community Residents (지역 주민의 음주 및 흡연 실태에 관한 연구)

  • Kim Keum-Ee
    • Journal of Korean Public Health Nursing
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    • v.20 no.1
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    • pp.39-56
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    • 2006
  • Objectives: To investigate the drinking and smoking habits among the community residents of a city. Methods: We surveyed 1.973 adults from October 1, 2005 to November 16, 2005. The data collected on alcohol drinking habits involved frequency, age of initiation, amount of alcohol consumed, number of attempts to reduce consumption and reasons for reducing it. The data collected on smoking habits involved amount, age of initiation, duration, situations, and the number of attempts to quit smoking. These were analyzed with frequency, percentage, mean, standard deviation, $x^2$-test, t-test and ANOVA. Results: The rate of drinking was 67.4%; 83.1% among males, and 52.5%among females. Among all drinkers, the largest group (29.8%) drank once or twice a week. The mean age of initiation was 21.2 years; 19.9 among males, and 22.8 among females. The average consumed amount was 5.8 glasses; males consumed 7.4 glasses, and females 3.3 glasses. The consumption was the highest among those in fifties. The rate of attempts to reduce drinking was 33.4%, mainly for health reasons. The rate of smoking was 38.0%; 72.4% among males, and 5.5% among females. Among all smokers, the largest group (44.1%) smoked between 11 and 20 cigarettes per day. The mean of the age of initiation was 20.7 years; 20.3 among males, 25.6 among females. The mean smoking duration was 20.9 years; 21.3 years among males, and 16.2 years among females. The main reason for the initial attempt was curiosity. The most common situation for smoking was after dinner. The rate of attempts to quit smoking was 77.1%, mainly out of concern for one's health. Alcohol consumption among smokers was higher than among any other group of nonsmokers. Also, the age of drinking initiation among smokers was lower than among any other group of nonsmokers. Conclusion: This study highlighted the actual data on drinking and smoking habits among community residents. The study can be utilized for creating programs aiming at reducing the consumption of alcohol and tobacco or stopping it altogether. Therefore, it is now possible to assess the indicators for interventions in each population group. Finally, the groups of heavy drinkers and smokers are expected to be given priority for intervention program.

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On the Maturity and Spawning of the Greenling, Hexagrammos grammus(Temminck et Schlegel) (노래미, Hexagrammos agrammus(Temminck et Schlegel)의 성성숙과 산란)

  • Chung, Ee-Yung;Kim, Sung-Yeon
    • Korean Journal of Ichthyology
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    • v.6 no.2
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    • pp.222-236
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    • 1994
  • Sexual maturation and spawning of the spottybelly greenling, Hexagrammos agrammus were histologically studied under photomicroscopy by considering changes in gonadosomatic index, hepatosomatic index and fatness, egg diameter composition, first sexual maturity and fecundity. The fish samples were collected monthly at the coastal area of Tongbaeksom, Pusan, Korea from July 1991 to July 1992. The gonadosomatic index(GSI) increased in September and reached the maximum value(female 4.31, male 1.61) in November when the gonad was mature and ripe. The values were decreased suddenly during the spawning season from December, and declined from January to August. The annual variations of hepatosomatic index(HSI) appeared to be correlated with those of GSI in female but were not significantly correlated in male. HSI in female began to increase in autumn with the increase of GSI, and reached the maximum in winter when the ovary was mature. Percentages of first sexual maturity in female and male fish were 50 % in 11.0~11.9 cm and 100 % in 13.0~13.9 cm groups. Both sexes participated in reproduction from one year old. H. agrammus was considered as a polycyclic species and spawns 3 times or more in the spawning season. Number of total and mature eggs in the absolute fecundity were proportional to standard length and body weight, respectively. Number of total and mature eggs in relative fecundity were also proportional to standard length, but rather decrease with increasing of body weight. Fatness coefficients reached the maximum value(female 15.32, male 15.14) in September(growing stage), and the values were sharply decreased after spawning. Thereafter, fatness values were gradually increased, therefore, the monthly changes in fatness coefficient closely correlated with the reproductive cycle. Sex ratios of female and male sexes of this species are showed 54.18 %, 45.82 %, respectively.

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Investigation of a nonreciprocal phase shift properties of optical waveguide isolators with a magneto-optic layer (자기 광학적층을 갖는 광 도파로 아이솔레이터 제작을 위한 비가역적 위상변위에 대한 연구)

  • Yang, Jeong-Su;Kim, Young-Il;Byun, Young-Tae;Woo, Deok-Ha;Lee, Seok;Kim, Sun-Ho;Yi, Jong-Chang
    • Korean Journal of Optics and Photonics
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    • v.14 no.2
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    • pp.142-145
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    • 2003
  • The nonreciprocal phase shift characteristics of infinite slab optical waveguides with magneto-optic materials in the cladding layer was calculated at 1.55 ${\mu}{\textrm}{m}$ for optical isolators. The infinite slab waveguide structures considered in this paper were as follows. rho magneto-optic materials used as a cladding layer were Ce:YIG and LNB(LuNdBi)$_3$(FeAl)$_{5}$)$_{12}$,). Their specific Faraday rotations Θ$_{F}$ are 4500$^{\circ}$/cm, 500$^{\circ}$/cm at wavelength 1.55 ${\mu}{\textrm}{m}$ respectively. The guiding layer with multi-quantum well structure was used, and it consists of 1.3Q and InGaAs. In order to investigate the effect of evanescent field penetrating the cadding, layer, guiding mode characteristics were calculated for the cases when the substrate is InP and air. We calculated the minimum lengths of 90$^{\circ}$ nonreciprocal phase shifters and their optimum guiding layer thicknesses in various optical waveguide structures.res.s.

A Neural Network for Long-Term Forecast of Regional Precipitation (지역별 중장기 강수량 예측을 위한 신경망 기법)

  • Kim, Ho-Joon;Paek, Hee-Jeong;Kwon, Won-Tae
    • Journal of the Korean Association of Geographic Information Studies
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    • v.2 no.2
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    • pp.69-78
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    • 1999
  • In this paper, a neural network approach to forecast Korean regional precipitation is presented. We first analyze the characteristics of the conventional models for time series prediction, and then propose a new model and its learning method for the precipitation forecast. The proposed model is a layered network in which the outputs of a layer are buffered within a given period time and then fed fully connected to the upper layer. This study adopted the dual connections between two layers for the model. The network behavior and learning algorithm for the model are also described. The dual connection structure plays the role of the bias of the ordinary Multi-Layer Perceptron(MLP), and reflects the relationships among the features effectively. From these advantageous features, the model provides the learning efficiency in comparison with the FIR network, which is the most popular model for time series prediction. We have applied the model to the monthly and seasonal forecast of precipitation. The precipitation data and SST(Sea Surface Temperature) data for several decades are used as the learning pattern for the neural network predictor. The experimental results have shown the validity of the proposed model.

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Preoperative Long Course Chemoirradiation in a Developing Country for Rectal Carcinoma: Kuala Lumpur Hospital Experience

  • Lee, Wei Ching;Yusof, Mastura Md.;Lau, Fen Nee;Ee Phua, Vincent Chee
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.6
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    • pp.3941-3944
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    • 2013
  • Background: The use of preoperative chemoirradiation is the commonest treatment strategy employed in Malaysia for locally advanced rectal cancer. We need to determine the local control and survival rates for comparison with established rates in the literature. Materials and Methods: This retrospective study analyzed all newly diagnosed patients with rectal adenocarcinoma who underwent long course preoperative radiotherapy (RT) at the Department of Radiotherapy and Oncology, Kuala Lumpur Hospital (HKL) between $1^{st}$ January 2004 and $31^{st}$ December 2010. The aim of the study was to determine the radiological response post radiotherapy, pathological response including circumferential resection margin (CRM) status, 3 years local control, 3 years overall survival (OS) and 3 years disease free survival (DFS). Statistical analysis was performed using the SPSS software. Kaplan-Meier and log rank analysis were used to determine survival outcomes. Results: A total of 507 patients with rectal cancer underwent RT at HKL. Sixty seven who underwent long course preoperative RT were eligible for this study. The median age at diagnosis was 60 years old with a range of 26-78 years. The median tumour location was 6 cm from the anal verge. Most patients had suspicion of mesorectum involvement (95.5%) while 28.4% of patients had enlarged pelvic nodes on staging CT scan. All patients underwent preoperative chemo-irradiation except for five who had preoperative RT alone. Only 38 patients underwent definitive surgery (56.7%). Five patients were deemed to be inoperable radiologically and 3 patients were found to have unresectable disease intraoperatively. The remaining 21 patients defaulted surgery (31.3%). The median time from completion of RT to surgery was 8 weeks (range 5.6 to 29.4 weeks). Fifteen patients (39.5%) had surgery more than 8 weeks after completion of RT. Complete pathological response was noted in 4 patients (10.5%). The pathological CRM positive rate after RT was 18.4%. With a median follow-up of 38.8 months, the 3 year local control rate was 67%. The 3 years rate for CRM positive (<2 mm), CRM clear (>2 mm) and pCR groups were 0%, 88.1% and 100% respectively (p-value of 0.007). The 3 year OS and DFS were 57.3% and 44.8% respectively. Conclusions: In conclusion, the approach of long course preoperative chemoirradiation for rectal cancer needs to be re-examined in our local setting. The high rate of local recurrence is worrying and is mainly due to patient defaulting post-preoperative chemoirradiation or delayed definitive surgery.

Enhancement of Whitening Effects of Lithospermum erythrorhizon Extracts by Ultra High Pressure (지치 초고압 추출물의 미백활성 증진)

  • Kim, Ji-Seon;Jeong, Myoung-Hoon;Choi, Woon-Yong;Seo, Yong-Chang;Ma, Choong-Je;Ahn, Ju-Hee;Kim, Nam-Seong;Hwang, Baik;Cho, Jeong-Sub;Lee, Hyeon-Yong
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.2
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    • pp.97-102
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    • 2011
  • In this study, whitening activity of Lithospermum erythrorhizon extracts were investigated according to several extraction processes: water extraction at $100^{\circ}C$ (WE100) and $60^{\circ}C$ (WE60), 70% ethyl alcohol extraction (EE) and ultra high pressure extraction (HPE) at 500 MPa for 30 minutes at $60^{\circ}C$. The extracts from ultra high pressure extraction showed the highest tyrosinase inhibition and melanogenesis inhibition activities as 52% and 79.5%, respectively, in adding $1mg/m{\ell}$ than others extraction processes. HPE extracts also showed the strong reducing power as 3.19 that absorbance at 450 mm. The contents of polyphenol in WE100, we measured as $10.1{\mu}g/m{\ell}$ in adding $1mg/m{\ell}$. Extracts have a high total flavonoid contents by HPE as $4.1{\mu}g/m{\ell}$ at $1mg/m{\ell}$. We can conclude that better whitening activity of extracts from high pressure extraction was due to high antioxidant activities which could be extracted by higher polyphenol and flavonoid contents in HPE than others.

Ultrastructural Study of Vitellogenesis during Oogenesis and Sexual Maturation of the Female Neptunea (Barbitonia) arthritica cumingii on the West Coast of Korea (한국 서해산 암컷 갈색띠매물고둥, Neptunea (Barbitonia) arthritica cumingii의 난자형성과정 중 난황 형성의 미세구조적 연구 및 성 성숙)

  • Chung, Ee-Yung
    • Development and Reproduction
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    • v.9 no.1
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    • pp.23-31
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    • 2005
  • Vitellogenesis during oogenesis, reproductive cycle and first sexual maturity of the female Neptunea (Barbitonia) arthritica cumingii was investigated by light and electron microscope observations. In the early vitellogenic oocyte, the Golgi complex and mitochondria were involved in the formation of lipid droplets and yolk granules. In late vitellogenic oocytes, the rough endoplasmic reticulum and multivesicular bodies were involved in the formation of proteid yolk granules in the cytoplasm. A mature yolk granule was composed of three components: main body(central core), superficial layer, and the limiting membrane. The spawning season was between May and August and the main spawning occurred between June and July when the seawater temperature rose to approximately $18{\sim}23^{\circ}C$. The female reproductive cycle can be classified into five successive stages: early active stage(September to October), late active stage(November to February), ripe stage(February to June), partially spawned stage(May to August), and recovery stage(June to August). The rate of individuals reaching the first sexual maturity was 53.1% in females of 51.0 to 60.9mm in shell height, and 100% in those over 61.0mm.

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