• Journal of Microbiology and Biotechnology
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• v.5 no.5
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• pp.257-263
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• 1995

As a first step for developing Lactobacillus strains capable of fermenting starch directly, the $\alpha$-amylase gene (amyL) from Bacillus licheniformis (Kim et al., 1988. Kor. J. Appl. Microbiol. Bioeng. 16: 369-373) was introduced into Lactobacillus casei strains and the level of $\alpha$-amylase expression in transformants was examined. 3 kb EcoRI fragments encompassing amyL were subcloned into the suitable lactococcal cloning vectors (pSA3, pMG36e, and p1L2530) and then recombinant plasmids were introduced into E. coli and L. casei strains by electroporation. Only one recombinant plasmid, $pIL2530\alpha$ was able to transform few L. casei strains tested at low efficiencies. The transformation efficiencies with the plasmid into L. casei YIT 9018 and L. casei A Tee 4646 were less than $10^2/\mu$ g pIL2530\alpha$. The level of amylase activities in L. casei was five to ten-fold lower than that in E. coli cells. $p1L2530\alpha$ was stably maintained in Lactobacillus strains in the presence of Em (5 $\mu $g/ml) but without antibiotic selection, it was unstable so more than 95$%$ of cells lost plasmids after a week of daily subculturing.

• Journal of the Korea Institute of Military Science and Technology
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• v.14 no.6
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• pp.1018-1025
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• 2011

The improved properties of corrosion for 90Cu10NiFe alloy in natural seawater were explained by sodium diethyl dithio carbamate(NaDDC), namely organic compound, which is reagent for heavy metal extractions of waste water. The efficiency of NaDDC as corrosion inhibitor for 90Cu10NiFe alloy has been investigated in seawater after immersion in various concentrations of NaDDC solutions for 12~36hrs at pH 8.2 by weight loss test and electrochemical techniques including potentiodynamic polarization and SEM-EDS measurements. The results showed that the corrosion resistance of 90Cu10NiFe alloy improves with the increasing concentration of NaDDC but it did not improves with increasing time any more, so the highest inhibition efficiency was 93% at 100mg/L, 36hrs. The results obtained from weight losses and corrosion rates in polarization curve measurements were in good agreement. Therefore, it showed that NaDDC is a good inhibitor for copper corrosion of 90Cu10NiFe alloy.

• Journal of Korean Society of Water and Wastewater
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• v.33 no.2
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• pp.131-140
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• 2019

This study used a packed column reactor and a horizontal flow mesh reactor to examine the removal of copper ions from aqueous solutions using pine bark, a natural adsorbent prepared from Korean red pine (Pinus densiflora). Both equilibrium and nonequilibrium adsorption experiments were conducted on copper ion concentrations of 10mg/L, and the removals of copper ions at equilibrium were close to 95%. Adsorption of copper ions could be well described by both the Langmuir and Freundlich adsorption isotherms. The bark was treated with nitric acid to enhance efficiency of copper removal, and sorption capacity was improved by about 48% at equilibrium; mechanisms such as ion exchange and chelation may have been involved in the sorption process. A pseudo second-order kinetic model described the kinetic behavior of the copper ion adsorption onto the bark. Regeneration with nitric acid resulted in extended use of spent bark in the packed column. The horizontal flow mesh reactor allowed approximately 80% removal efficiency, demonstrating its operational flexibility and the potential for its practical use as a bark filter reactor.

• Clean Technology
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• v.28 no.1
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• pp.32-37
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• 2022

Food waste is produced from food factories, food services, and home kitchens. The generated mass reached 5.4 million tons/year in 2020. The basic management technology for such waste has been biological degradation under an anaerobic environment. However, the whole process is intrinsically slow and considerably affected by the inner physicochemical properties of the waste and other surrounding conditions, which makes optimization of the process difficult. The most promising options to counter this massive generation of waste are eco-friendly treatments or recycling. As a preliminary step for these options, attempts were made to evaluate the feasibility and usability of three simulative models based on reaction kinetics. Model (A) predicted relative changes over reaction time for reactant, intermediate, and product. Overall, an increased reaction rate produced less intermediate and more product, thereby leading to a shorter total reaction time. Particle diminishing model (B) predicted reduction of the total waste mass. The smaller particles diminished faster along with the dominant effect of microbial reaction. In Model (C), long-chain cellulose was predicted to transform into reducing sugar. At a standard condition, 48% of cellulose molecules having 10⁵ repeating units turned into reducing sugar after 100 h. Also it was found that the optimal enzyme concentration where the highest amount of remnant sugar was harvested was 1 mg L^-1.

• Advances in concrete construction
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• v.13 no.3
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• pp.233-242
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• 2022

An attempt is made to develop an eco-friendly concrete with ground granulated blast furnace slag (GGBS) and pond ash as partial replacement materials for cement and fine aggregate, respectively without compromising the strength and durability. Sixteen concrete mixes were developed by replacing cement and fine aggregate by GGBS and pond ash, respectively in stages of 10%. The maximum replacement levels of cement and fine aggregates were 50% and 30% respectively. Experimental results revealed that the optimum percentage of GGBS and pond ash replacement levels were 30% and 20% respectively. The optimized mix was used further to study the flexural behavior and durability properties. Reinforced Concrete (RC) beams were cast and tested under a four-point bending configuration. Also, the specimens prepared from the optimized mix were subjected to alternate wet and dry cycles of acid (3.5% HCl and H₂SO₄) and sulphate (10% MgSO₄) solutions. Results show that the optimized concrete mix with GGBS and pond ash had a negligible weight loss and strength reduction.

• Corrosion Science and Technology
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• v.23 no.2
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• pp.139-144
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• 2024

Recently, due to the rapid spread and continuation of COVID-19, customer demand for health and hygiene has increased, requiring the development of new products that express antiviral and antibacterial properties. In particular, viruses are much smaller in size than bacteria and have a fast propagation speed, making it difficult to kill. POSCO has developed eco-friendly PCM color coated steel sheets with excellent antiviral properties by introducing inorganic composite materials to the color coating layer on the surface of Zn-Al-Mg alloy plated steels. The virus is not only destroyed by adsorption of metal ions released from the surface of the coating film, but is also further promoted by the generation of reactive oxygen species by the reaction of metal ions and moisture. As a result of evaluating the developed products under the International Standard Evaluation Act, the microbicidal activity was 99.9% for viruses, and 99.99% for bacteria and 0% fungi. In particular, excellent results were also shown in the durability evaluation for life cycle of the product. The developed product was applied as a wall of school classrooms and toilets and ducts for building air conditioning, resulting in excellent results. Developed products are being applied for construction and home appliances to practice POSCO's corporate citizenship.

• Electronics and Telecommunications Trends
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• v.39 no.1
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• pp.83-94
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• 2024

There have been continuous requirements for developing more reliable energy storage systems that could address unsolved problems in conventional lithium-ion batteries (LIBs) and thus be a proper option for large-scale applications like energy storage system (ESS). As a promising solution, aqueous metal-ion batteries (AMIBs) where water is used as a primary electrolyte solvent, have been emerging owing to excellent safety, cost-effectiveness, and eco-friendly feature. Particularly, AMIBs adopting mutivalence metal ions (Ca²⁺, Mg²⁺, Zn²⁺, and Al³⁺) as mobile charge carriers has been paid much attention because of their abundance on globe and high volumetric capacity. In this research trend review, one of the most popular AMIBs, zinc-ion batteries (ZIBs), will be discussed. Since it is well-known that ZIBs suffer from various (electro) chemical/physical side reactions, we introduce the challenges and recent advances in the study of ZIBs mainly focusing on widening the electrochemical window of aqueous electrolytes as well as improving electrochemical properties of cathode, and anode materials.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.2
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• pp.317-324
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• 2012

To reduce non-point source pollutants in Juam Lake eco-wetlands, purification efficiencies of pollutants were investigated at three different systems. The constructed wetlands (CWs) consisted of A system, B system and C system. A system consisted of $1^{st}$ free water surface (FWS) CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ subsurface flow (SSF) CW and $5^{th}$ SSF CW. B system consisted of $1^{st}$ FWS CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ FWS CW and $5^{th}$ SSF CW. C system consisted of $1^{st}$ FWS CW, $2^{nd}$ FWS CW, $3^{rd}$ FWS CW, $4^{th}$ FWS CW and $5^{th}$ SSF CW. The concentrations of BOD, COD, SS, T-N and T-P in inflow ranged 0.20 ~ 0.91, 1.24 ~ 8.00, 0.60 ~ 8.60, 0.04 ~ 2.50 and $0.001{\sim}0.685mg\;L^{-1}$ from March to October in 2011, respectively. Removal rates of BOD, SS, T-N and T-P were high in Autumn, Spring, Spring and Summer, respectively. In A system, $1^{st}$ FWS CW, $2^{nd}$ FWS CW and $3^{rd}$ FWS CW were dominated by Leersia oryzoides. In B system, $1^{st}$ FWS CW, $3^{rd}$ FWS CW and $4^{th}$ FWS CW were dominated by Leersia oryzoides. In C system, $2^{nd}$ FWS CW and $3^{rd}$ FWS CW were dominated by Nymphaea teragona.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.5
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• pp.418-428
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• 2007

Physical and chemical properties of the aggregate by-products including sludge and crushed dust samples collected from the 21 private companies throughout the country were analyzed to evaluate possible usage of the by-products as artificial soil materials for plantation. The pH of the materials ranged from 8.0 to 11.0. The organic matter content was $2.85g\;kg^{-1}$, and the total nitrogen content and available phosphate content were low as 0.7 percents and $12.98mg\;kg^{-1}$, respectively. Exchangeable $Ca^{2+}$, $Mg^{2+}$, $K^+$, and $Na^+$ were 2.29, 0.47, 0.02 and $0.05cmol\;kg^{-1}$, respectively. Heavy metal contents were lower than the limits regulated by environmental law of Korea. Textural analysis showed that most of the materials were silt loam with low water holding capacity ranged from 0.67 to 7.41 percents, and with low hydraulic conductivity ranged from 0.4 to $2.8m\;s^{-1}$. Mineralogical analysis showed that the aggregate by product materials were mostly composed of silicate, alumina and ferric oxides except calcium oxide dominant materials derived from limestones. The primary minerals were quartz, feldspars and dolomites derived from granite and granitic gneiss materials. Some samples derived from limestone material showed calcite and graphite together with the above minerals. According to the result, it can be concluded that the materials could be used as the artificial soil material for plantation after proper improvement of the physico-chemical properties and fertility.

• Journal of Korean Society of Water and Wastewater
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• v.19 no.4
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• pp.497-505
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• 2005

Domestic Sewage Treatment Plants are mostly based on biological treatment, in which large amounts of excess sludge are generated and occupy about 40 ~ 60% of the total sewage treatment costs. Several methods for sludge treatment has been so far reported as upgrading biodegradation of sludge; heat treatment, chemical treatment, including thermo-alkali and ozone, mechanical treatment including ultrasonic pulverization. But, it has a limitation in case of reducing the amount of excess sludge which are already producted. In this study, application of excess sludge reduction process using thermophilic aerobic bacteria for activated sludge was examined. The research was carried out two different stage. one for a biological wastewater treatment and the other for a thermophilic aerobic solubilization of the waste sludge. A portion of excess sludge from the wastewater treatment step was into the thermophilic aerobic sludge solubilization reactor, in which the injected sludge was solubilized by thermophilic aerobic bacteria. The solubilized sludge was returned to the aeration tank in the wastewater treatment step for its further degradation. Sludge solubilization reactor was operated at $63{\pm}2^{\circ}C$ with hydraulic retention time(HRT) of 1.5 ~ 1.7 day. Control group was operated with activated sludge process(AS) and experiment group was operated with three conditions(RUN 1, RUN 2, RUN3). RUN 1 was operated with AS without sludge solubilization reactor. RUN 2 were operated with AS with sludge solubilization reactor to examine correlation between sludge circulation ratio and sludge reduction ratio by setting up sludge circulation ratio to 3. RUN 3 was operated with sludge circulation ratio of 3 and MLSS concentration of 1,700~2,000mg/L to examine optimum operation condition. The quantity of excess sludge production was reduced sharply and in operation of RUN 3, sludge The quantity of excess sludge production was reduced sharply and in operation of RUN 3, sludge solubilization ratio and sludge reduction ratio were 53. 7%, 95.2% respectively. After steady state operation, average concentration of TBOD, SBOD, $TCOD_{Cr}$, $SCOD_{Cr}$, TSS, VSS, T-N, T-P of effluent were 4.5, 1.7, 27 .8, 13.8, 8.1, 6.2, 15.1, 1.8mg/L in the control group and were 5.6, 2.0, 28.6, 19.1, 9.7, 7.2, 16.1, 2.0mg/L in the experimental group respectively. They were appropriate to effluent standard of Sewage Treatment Plants.