• Mycobiology
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• v.31 no.1
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• pp.23-31
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• 2003

With universal primer ITS1-F, the specific DHJ2 primer was developed to detect the Ectomycorrhizal(ECM) root tips in soil and to identify the species of ECM fungi, as based on DNA sequences of rDNA stored in GeneBank of NCBI. This primer was designed with the common sites of rDNA of Amanita and Boletus, and was also designed with several DNA programs provided by NCBI. The DNA fragments synthesized by PCR were calculated to be 1,000 to 1,200 bps of DNA located to 18s to 28s rDNA to contain two variable sites of ITS, indicating much diversities for specific species or ecotypes of ECM fungi. The primer DHJ2 reacted with the genomic DNA's extracted from the tissues of basidiocarp at the rate of 73 of 80 fungi collected produced single bands with a 1,100 bps length. The DNA fragment synthesized with the genomic DNA that extracted from eight ECM tips of Pinus densiflora was confirmed and analysized to the rDNAs of ECM in full sequences, and informed to be a ECM fungal species in the forest.

• Mycobiology
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• v.41 no.2
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• pp.77-81
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• 2013

This study aimed to investigate differences in ectomycorrhizal (ECM) fungal communities between disturbed sites and undisturbed old forest sites. ECM root tips of Pinus densiflora were collected from 4 sites disturbed by human activities and 3 undisturbed old forest sites adjacent to the disturbed sites. Results in this study showed that the number of ECM root tips, species diversity, and number of species were significantly higher in the disturbed sites than in the undisturbed sites, suggesting that the ECM fungal community structure was affected by the degree of disturbance.

• The Korean Journal of Mycology
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• v.47 no.3
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• pp.199-208
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• 2019

In this study, the community structures of the ectomycorrhizal (ECM) fungi in the roots of Abies koreana and Taxus cuspidata were investigated at different altitudes of Mt. Halla. We identified the collected ECM root tips based on morphological characteristics and phylogenetic analysis through sequencing of the rDNA ITS regions. From the roots of A. koreana and T. cuspidata, 11 species and 12 species were identified, respectively. The Shannon's index and species evenness and abundance of the ECM fungi were higher in the higher than lower regions, regardless of host plant species, however, the number of ECM root tips showed the opposite pattern. The community similarity among the ECM fungi in A. koreana was significantly higher than that among the ECM fungi in T. cuspidata or than that between A. koreana and T. cuspidata (p < 0.05). These results could be useful for the conservation and management of the habitat of A. koreana, which is threatened with extinction due to increasing ambient air temperature.

• Journal of Ecology and Environment
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• v.32 no.1
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• pp.13-18
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• 2009

This study was conducted to investigate ectomycorrhizal (ECM) diversity on Pinus densiflora seedlings in a disturbed pine forest. Pine seedlings less than one year old were collected from disturbed and undisturbed sites in the Mt. Songni region. The belowground ECM fungal communities colonizing P. densiflora seedlings were studied using morphotyping and DNA sequencing. The relative abundance of ECM root tips was significantly higher in the undisturbed sites than in the disturbed sites, and the ECM species diversity was lower in the disturbed sites than in the undisturbed sites. In addition, the ECM community composition was significantly different in the disturbed and undisturbed forest sites.

• Journal of Ecology and Environment
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• v.29 no.2
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• pp.143-149
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• 2006

This study was conducted to identify native ectomycorrhizal (ECM) fungi colonizing Pinus densiflora for revegetation of abandoned coal mines in Korea. Seedlings of P. densiflora growing on coal mining spoils of a study site in Samcheok were collected. ECM roots were observed under stereomicroscope and their DNA were extracted from each root tip for a seedling for molecular identification. A PCR primer pair specific to fungi, ITS1F and ITS4, was used to amplify fungal DNA. Restriction enzymes, Alul and Hinfl were used for restriction fragment length polymorphism (RFLP). Combined with RFLP profiles and sequence analysis, total twenty one taxa were identified from the ECM root tips. Basidiomycetous fungi including Thelephoraceae, Pezizales, Laccaria, Pisolithus and Ascomycetous fungi including ericoid mycorrhizal fungi were identified from this study. Results showed that the most frequently found in the study sites was a species in Thelephoraceae. A possible use of ECM fungi identified in this study for the revegetation of abandoned coal mines with P. densiflora was discussed.

• The Korean Journal of Mycology
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• v.37 no.2
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• pp.134-138
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• 2009

This study was conducted to investigate colonization of ectomycorrhizal fungi(ECM) in roots of Abies koreana which is an endemic and endangered species in Korea. Roots of A. koreana were collected at Mt. Halla. ECM root tips were classified using morphotyping and identified using sequences of internal transcribed spacer (ITS) region of the fungal rDNA. Total 8 species of ECM fungi were identified from roots of 11 seedlings of A. koreana : Cenococum geophilum, Russula brevipes, 2 species of Russula, 2 species of Thelephora, Cortinarius camphorates and 2 species of Helotiales. These species were known to be typical ectomycorrhizal fungi found in coniferous mature forests.

• Journal of Korean Society of Forest Science
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• v.99 no.2
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• pp.172-178
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• 2010

The status of ectomycorrhizal (ECM) fungal colonization in Pinus thunbergii seedlings was investigated 2 years after planting in an eastern coastal area of Korea. We established three $10{\times}10$ m plots at a P. thunbergii plantation in Gangneung and sampled lateral roots from 10 seedlings in each plot. ECMs were classified into morphological groups and the number of root tips of each morphotype was counted. In total, 8 ECM morphotypes were observed and fungal species that form each morphotype were identified by sequencing of the internal transcribed spacer (ITS) region of the nuclear rDNA. Suillus granulatus was the most abundant species (44.1-65.7% of relative abundance) in all plots, followed by Tomentella ellisii (14.0-37.8%) and unidentified fungus belonged to Atheliaceae (10.6-20.1%). These 3 fungal species accounted for almost all of the ECM abundance in each plot (94.9-99.8%). The remaining 5 fungal species were uncommon and rare. There was no clear difference in ECM fungal communities among plots. Community structure of ECM fungi in the young P. thunbergii plantation was simple and composed of fungal species that were also observed in mature coastal pine forests.

• Mycobiology
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• v.34 no.4
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• pp.191-195
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• 2006

This study was conducted to investigate the different effects of ectomycorrhizal fungal (ECMF) species on the growth of seedlings of Pinus densiflora, and the effects of ECMF diversity on plant productivity. A total of five species of ECMF were isolated from root tips of pine seedlings collected from Mt. Songni and used as inocula. Pots containing pine seedlings were inoculated with either a single ECMF species or a mixture of five ECMF species. All of the seedlings formed ECM on their roots except for the control plants. The pine seedlings' growth responses varied by the different ECMF species. Also, pine seedlings inoculated with a mixture of five ECMF species showed the highest growth response. The results of the study suggest that the colonization of diverse species of ECMF will increase plant productivity, and the selection of suitable ECMF species could be an important factor for plant growth.