• 한국식품저장유통학회지
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• 제22권2호
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• pp.290-296
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• 2015

해양생물 유래 기능성 소재 개발 연구의 일환으로, 국내 자생하는 감태 외 9종(곰피, 대황, 매생이, 모자반, 미역, 지충이, 청각, 톳, 파래)의 해조류을 이용하여 열수 및 에탄올 추출물을 제조하여 추출물의 항산화 활성 및 ${\alpha}$-glucosidase 저해활성을 조사하였다. 총 페놀 및 플라보노이드 함량은 대황 에탄올 추출물에서 각각 150.81, 77.02 mg/g으로 가장 많았다. DPPH radical 소거능은 대황, 감태, 지충이 에탄올 추출물이 각각 86.26, 78.72, 68.32%를 나타내었으며, ABTS radical 소거능은 대황, 감태, 지충이 열수 및 에탄올 추출물에서 모두 99% 이상의 높은 활성을 나타내었다. SOD 유사활성과 환원력은 대황 에탄올 추출물이 21.34%, 1.710($OD_{700}$)으로 가장 높았으며, 지방산패억제능도 대황 에탄올 추출물에서 83.95%로 가장 높았다. 또한, 해조류 추출물의 ${\alpha}$-glucosidase 저해 활성은 대황, 감태, 지충이 열수 추출물에서 97.75, 95.17, 87.13%를 나타내었으며, 에탄올 추출물에서는 3종류 모두 약 98%의 높은 저해활성을 나타내었다. 해조류 중 대황, 감태, 지충이의 항산화 활성 및 ${\alpha}$-glucosidase 저해활성이 우수하여 천연 기능성 소재 개발을 위한 좋은 원료가 될 수 있을 것으로 사료되며, 기능성소재로 사용하기 위하여 적용할 제품에 따라 추출물의 재료, 첨가농도, 제품의 생리활성 등과 관련된 광범위한 연구가 요구된다.

• Journal of Nutrition and Health
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• 제39권4호
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• pp.381-391
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• 2006

This study is performed to compare the dietary intakes and food sources of copper (Cu) using the database of Cu content developed in this study between the elementary schoolchildren in remote rural areas (RA, n=58, $9.9{\pm}1.7$ yrs) and those in urban area (UA, n=60, $9.4{\pm}1.8$ yrs), and to analyze the relationship between serum Cu concentration and serum antioxidant status in the RA. The results obtained in this study were as followings: 1) Dietary intakes of calorie, calcium and iron in the RA were in the 3/5-4/5 of the Korean RDA while the UA were similar to or more than the Korean RDA, 7th ed. except iron. 2) More than 273 kinds of food consumed by the subjects were analyzed the content of Cu and database of Cu content were developed in the present study. The mean dietary intake of Cu per day in the RA was $0.99{\pm}0.07mg/d$ ($170.0{\pm}13.2%$ of the USA RDA) while it was $1.22{\pm}0.07mg/d$ ($203.4{\pm}13.1%$ of the RDA) in the UA. The percentage of dietary intakes of Cu less than 213 of the RDA was 8.6% in the RA in comparison to 0% in the UA. 3) The RA and the UA consumed more than 80% of total dietary intakes of Cu from plant foods. Thus, the RA and the UA consumed Cu from cooked rice, vegetables and fruits as a major source. However the RA had less Cu from meat and their products than did the UA (p<0.05) .4) Crab stew including crab and juice was the highest food source of Cu for the total subjects, followed by seasoned bud of aralia, cooked; beef rib meat, roasted; soybean paste soup w/mallow; and soybean paste soup w/mallow & beef. Major food source of Cu was similar for the RA and the UA such as cooked rice, vegetables and fruits. 5) Mean concentration of serum Cu in the RA was $18.1{\pm}0.7{\mu}M/L$ that was in the normal value, and all subjects in this group were in more than normal value. In the RA serum Cu concentration related positively with serum ceruloplasmin concentration, serum vitamin C concentration and EC SOD activity, respectively. However, serum Cu concentration did not relate with serum TBARS concentration in the RA. Above results showed that the RA had good status of Cu nutrition based upon dietary intake and serum concentration, however some of the RA had lower intake of Cu than the RDA. The overall children in the UA had good Cu nutrition. Therefore, the subgroup of the RA should be supported to improve their Cu nutrition, and this support could give them better antioxidant status based upon positive relationship between serum Cu concentration and serum antioxidant status in the RA.

• 한국환경과학회지
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• 제24권7호
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• pp.917-925
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• 2015

Styela clava tunic is generated in large amounts as a waste from S. clava processing plants and leads to environmental problems. It destroys the beach scenery and causes a bad smell and pollution by trashing on the seashore. Therefore, purpose of this study was to investigate antioxidant and antihypertensive activities of different solvent extracts from S. clava tunic for recycling of fishery waste. Antioxidant and antihypertensive activities of all extracts were concentration-dependent. Of extracts, hot water extract showed the highest DPPH radical scavenging activity with the lowest effective concentration ($EC_{50}$) value (0.733 mg/ml). Chloroform extract exhibited the highest metal chelation activity with the lowest $EC_{50}$ value (2.696 mg/ml). Autoclaved water extract showed the highest NO radical scavenging activity with the lowest $EC_{50}$ value (0.491 mg/ml) and n-hexane extract showed the highest reducing power ($A_{700}=1.897$ at 100 mg/ml). And n-butanol extract showed the highest SOD-like activity with the lowest $EC_{50}$ value (19.116 mg/ml) and ACE inhibition activity with the lowest inhibitory concentration($IC_{50}$) value (0.149 mg/ml). These results indicate that extracts obtained from S. clava tunic may potential candidate to reduce diseases caused by various oxidative stresses and hypertension.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2010년도 정기총회 및 추계학술발표회
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• pp.14-14
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• 2010

Vitamin C (ascorbic acid) is an essential component for collagen biosynthesis and also for the proper functioning of the cardiovascular system in humans. Unlike most of the animals, humans lack the ability to synthesize ascorbic acid on their own due to a mutation in the gene encoding the last enzyme of ascorbate biosynthesis. As a result, vitamin C must be obtained from dietary sources like plants. In this study, we have developed two different kinds of transgenic potato plants (Solanumtuberosum L. cv. Taedong Valley) overexpressing strawberry GalUR and mouse GLoase gene under the control of CaMV 35S promoter with increased ascorbic acid levels. Integration of the these genes in the plant genome was confirmed by PCR and Southern blotting. Ascorbic acid(AsA) levels in transgenic tubers were determined by high-performance liquid chromatography(HPLC). The over-expression of these genes resulted in 2-4 folds increase in AsA intransgenic potato and the levels of AsA were positively correlated with increased geneactivity. The transgenic lines with enhanced vitamin C content showed enhanced tolerance to abiotic stresses induced by methyl viologen(MV), NaCl or mannitol as compared to untransformed control plants. The leaf disc senescence assay showed better tolerance in transgenic lines by retaining higher chlorophyll as compared to the untransformed control plants. Present study demonstrated that the over-expression of these gene enhanced the level of AsA in potato tubers and these transgenics performed better under different abiotic stresses as compared to untransformed control. We have also investigated the mechanism of the abiotic stress tolerance upon enhancing the level of the ascorbate in transgenic potato. The transgenic potato plants overexpressing GalUR gene with enhanced accumulation of ascorbate were investigated to analyze the antioxidants activity of enzymes involved in the ascorbate-glutathione cycle and their tolerance mechanism against different abiotic stresses under invitro conditions. Transformed potato tubers subjected to various abiotic stresses induced by methyl viologen, sodium chloride and zinc chloride showed significant increase in the activities of superoxide dismutase(SOD, EC 1.15.1.1), catalase, enzymes of ascorbate-glutathione cycle enzymes such as ascorbate peroxidase(APX, EC 1.11.1.11), dehydroascorbate reductase(DHAR, EC 1.8.5.1), and glutathione reductase(GR, EC 1.8.1.7) as well as the levels of ascorbate, GSH and proline when compared to the untransformed tubers. The increased enzyme activities correlated with their mRNA transcript accumulation in the stressed transgenic tubers. Pronounced differences in redox status were also observed in stressed transgenic potato tubers that showed more tolerance to abiotic stresses when compared to untransformed tubers. From the present study, it is evident that improved to lerance against abiotic stresses in transgenic tubers is due to the increased activity of enzymes involved in the antioxidant system together with enhanced ascorbate accumulated in transformed tubers when compared to untransformed tubers. At moment we also investigating the role of enhanced reduced glutathione level for the maintenance of the methylglyoxal level as it is evident that methylglyoxal is a potent cytotoxic compound produced under the abiotic stress and the maintenance of the methylglyoxal level is important to survive the plant under stress conditions.

• Archives of Reconstructive Microsurgery
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• 제15권1호
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• pp.1-9
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• 2006

The purpose of this study is to determine the effects of selective inducible nitric oxide synthase (iNOS) inhibitor N-[3-aminomethyl]benzyl]acetamidine (l400W) on the reperfused cremaster muscle. The extracellular superoxide dismutase knockout ($EC-SOD^{-/-}$) mice was used to make the experimental window for ischemia-reperfusion injury. The muscle was exposed to 4.5 h of ischemia followed by 90 min of reperfusion and the mice received either 3 mg/kg of 1400W or the same amount of phosphate buffered saline (PBS) subcutaneously at 10 min before the start of reperfusion. The results showed that 1400W treatment markedly improved the recovery of the vessel diameter and blood flow in the reperfused cremaster muscle compared to that of PBS group. Histological examination showed reduced edema in the interstitium and muscle fiber, and reduced nitrotyrosine formation (a marker of total peroxinitrite ($ONOO^-$) in 1400W-treated muscle compared to PBS. Our results suggest that iNOS and $ONOO^-$ products are involved in skeletal muscle I/R injury. Reduced I/R injury by using selective inhibition of iNOS is perhaps via limiting cytotoxic $ONOO^-$ generation, a reaction product of nitric oxide (NO) and superoxide anion ($O_2^-$). Thus, inhibition of iNOS appears to be a good treatment strategy in reducing clinical I/R injury.

• 한국수산과학회지
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• 제43권3호
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• pp.188-194
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• 2010

The in vitro antioxidant activities of five melania snails in the genus Semisulcospira (S. coreana, S. forticosta, S. libertina, S. tegulata and S. gottschei) were tested in detail. The total phenolic contents of the snails ranged from $32.3{\pm}1.0$ to $87.9{\pm}6.9$ mg gallic acid equivalent (GAE)/g dry sample. The $EC_{50}$ values for the 2,2-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities were $2.245{\pm}0.179$ mg/mL for S. coreana and $9.319{\pm}1.276$ mg/mL for S. gottschei and differed significantly (P<0.05) among the tested species. The superoxide dismutase (SOD)-like activity was highest for S. gottschei at 67.2% and lowest for S. forticosta at 4.7%. However, no significant differences among the species were recognized for the peroxynitrite anion scavenging activity. Comparing the correlation coefficients between the total phenolic contents and the DPPH radical and peroxynitrite anion scavenging activities, there was a low level relationship between each activity.

• 한국환경농학회지
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• 제33권1호
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• pp.9-16
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• 2014

BACKGROUND: This study was conducted to investigate the effects of seeding timing and method of rye and/or hairy vetch on seasonal soil and foliar nutrient concentrations as well as fruit quality in a pesticide-free pear orchard. METHODS AND RESULTS: The treatments included as followed: single seeding of rye in September (Sep-Mono), November (Nov-Mono), and January (Jan-Mono), or mix seeding of rye+hairy vetch in November (Nov-Mix) and January (Jan-Mix), or sod culture as a control. Cover crops or vegetation was mown and mulched on the soil surface in April and May for two years. Nov-Mix treatment produced the highest dry matter weight of $12,070kg\;ha^{-1}$, with the lowest dry matter weight for sod culture ($6,520kg\;ha^{-1}$), following Jan-Mix ($7,030kg\;ha^{-1}$). Nov-Mix treatments increased potential amount of N, P, and K from the raw materials of the cover crops as well as improved soil physical properties. Nov-Mix treatments overall elevated soil pH, EC, organic matter, and $P_2O_5$ in May compared to other cover crop treatments or sod culture. The difference of the seasonal nutrient concentrations in leaves or fruit qualities were not consistently occurred amongst treatments. CONCLUSION: Nov-Mix treatments showed playing role in a substitute of a chemical fertilizer. Delayed seeding of cover crops such as Jan-Mix did not increase the potential dry matter production due to the short growing period, and the seeding time would affect the dry matter production of cover crops.

• Nutrition Research and Practice
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• 제3권3호
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• pp.208-211
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• 2009

This study investigated the effect of vitamin $B_6$ deficiency on antioxidant enzyme activities and lipid profile in rats with exercise-induced oxidative stress. Forty eight rats were fed either a vitamin $B_6$ deficient diet (B6-) or a control diet (control) for 4 weeks and then subdivided into 3 groups: pre-exercise (PreE); post-exercise (PostE); recess after exercise (recessE). Compared to those of control group, plasma catalase and hepatic cytosol superoxide dismutase (SOD, EC 1.15.1.1) activities of B6- group were lower regardless of exercise. The ratio of reduced glutathione/oxidized glutathione (GSH/GSSG) of B6 - group was lower in PreE and there was no difference between PostE and recessE. The level of malondialdehyde (MDA) of B6- was significantly higher in PreE and PostE. High-density lipoprotein-cholesterol (HDL-C) level of B6- group was lower regardless of exercise. Atherosclerotic index of $B_6$- group was higher in PreE and there was no difference between PostE and recessE. It is suggested that a reduction in antioxidative status caused by vitamin $B_6$ deficiency may be aggravated under exercise-induced oxidative stress.

• 한국식품영양과학회지
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• 제45권10호
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• pp.1447-1452
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• 2016

본 연구는 항산화능이 우수한 것으로 알려진 쇠비름을 선택하여 쇠비름 페이스트를 만든 후 0, 1, 3, 5%를 첨가한 설기떡을 제조하여 품질 특성 및 항산화성을 분석하였다. 쇠비름 첨가량이 증가할수록 산도와 환원당이 증가하였다. 색도는 쇠비름 첨가량이 증가할수록 명도는 감소하고, 적색도와 황색도는 증가하였다. 총페놀 함량은 쇠비름 첨가량이 증가할 수록 증가하였다. DPPH 라디칼 소거능과 superoxide dismutase 유사 활성은 쇠비름 첨가량이 증가할수록 $IC_{50}$ 값과 $EC_{50}$ 값이 낮아져 항산화능이 높았다. 기호도는 쇠비름을 첨가할수록 외관의 기호도는 증가하지만 향미, 맛, 조직감, 전체적 기호도는 3% 쇠비름 첨가군이 가장 높게 나타났고, 5% 쇠비름 첨가군이 감소함을 보였다. 특히 전체적 기호도는 쇠비름 3% 첨가군이 가장 높은 점수를 받았다. 이상의 결과로 보아 쇠비름 첨가가 떡의 항산화능과 기호도를 증가시켜 설기떡의 가치를 높일 수 있을 것으로 보인다. 이 연구를 통해 쇠비름을 첨가한 설기떡의 항산화능이 우수한 것을 볼 수 있었다. 또한, 쇠비름의 향미와 맛을 고려하여 볼 때 쇠비름을 3% 첨가하여 제조하면 외관, 향미, 맛, 전체적 기호도 등 선호도 측면에서 적당할 것으로 생각한다.

• 한국식품영양과학회지
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• 제40권5호
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• pp.625-634
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• 2011

본 연구는 녹차씨껍질 분획 추출물 중 염증 저해능이 강력한 EtOAC분획을 선정하여 대식세포주인 RAW264.7 macrophage cell에서 항염증효과의 기전을 생화학적, 분자학적수준에서 분석하고자 하였다. 녹차씨껍질 추출물 100 mg당 총 페놀함량은 EtOAC분획에서 가장 높은 수준이었으며 EtOH추출물>PE분획>BuOH분획과 $H_2O$분획의 순으로 나타났다. 또한 EtOAC분획의 NO 억제능이 가장 강한 것으로 나타나, EtOAC분획의 polyphenol을 분석한 결과 EGC ($1146.5{\pm}11.01\;{\mu}g/g$)> tannic acid($967.0{\pm}32.24\;{\mu}g/g$)> EC ($70.9{\pm}4.39\;{\mu}g/g$)> gallic acid($947.6{\pm}1.03\;{\mu}g/g$)> caffeic acid($37.7{\pm}1.46\;{\mu}g/g$)> ECG($35.5{\pm}3.19\;{\mu}g/g$)> EGCG($15.5{\pm}0.09\;{\mu}g/g$)의 순으로 나타났다. 녹차씨껍질 EtOAC분획이 RAW264.7 macrophage cell에서 LPS 처리에 의한 산화적 스트레스로 발생되는 NO 생성을 농도 의존적으로 감소시키며($IC_{50}$: $80.11\;{\mu}g/mL$) $PGE_2$의 생성을 억제하였다. 염증생성 전사인자인 iNOS의 유전자 발현은 농도 의존적으로 억제시켰으나 COX-2의 단백질 발현에는 영향을 미치지 않았다. 녹차씨껍질 EtOAC분획은 총 항산화능과 GSH 수준을 증가시켜 산화적 스트레스를 경감시키는 역할을 하며 항산화효소계인 catalase, GSH-red 및 Mn-SOD 활성의 단백질 발현을 증가시키는 것으로 나타났다. 핵에서의 p65 농도는 대조군에 비해 녹차씨껍질 EtOAC분획을 처리한 군에서 현저하게 낮은 것으로 나타났다. 이상의 결과에서, 녹차씨껍질 EtOAC분획은 NF-${\kappa}B$ 활성을 억제함으로써 iNOS 단백질 발현을 억제하여 NO의 생성을 감소시키고 총 항산화능과 GSH 수준을 증가시키며, 항산화 효소계를 활성화시켜 세포내 산화적 스트레스를 감소시킴으로써 LPS 자극에 의한 염증반응을 지연하거나 억제하는 것으로 사료된다.