• Clinical and Experimental Reproductive Medicine
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• v.37 no.1
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• pp.13-23
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• 2010

Objective: The present study was carried out to induce differentiation of human embryonic stem cells (hESCs) into germ cells and to establish a culture condition for single hESCs dissociated by enzyme. Methods: Embryonic body (EB) was formed by hanging drop culture for 3 days from hESCs colony. The EBs were cultured in the medium supplemented with retionic acid (RA) or/and bone morphogenetic protein-4 (BMP4) for 14 days to differentiate into germ cells. Germ cell specific markers, c-kit and VASA were used for immunohistochemistry of EB. Human ESCs colonies were dissociated into single cells by Collagenase, Tryple and Accutase, and then colony formation rate of the single cells was examined. Rho-associated kinase inhibitor (ROCK inhibitor, Y27632) was added into the culture medium of single cells to reduce the apoptotic damage during the dissociation. Results: Single cells dissociated with Tryple or Accutase showed higher colony formation rates compared to the cells dissociated with Collagenase. Seeding of $5{\times}10^3$ cells/well (4 well dish) was efficient to obtain high colony formation rate compared to other concentrations of seeding cell. Addition of Y27632 significantly increased the colony formation rate of the single cells dissociated by Tryple. Immunohistochemistry of EB with c-kit and VASA markers showed a weak fluorescence signals compared to the signals from the testicular tissue. Conclusion: Dissociation with Tryple was useful to obtain healthy single cells and addition of Y27632 was beneficial for survival and colony formation of the single cells. Unlike other studies, we just observed a dim fluorescence staining of the germ cell markers, probably caused by the short-term culture for the differentiation of EB compared to other studies.

• Journal of Ecology and Environment
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• v.29 no.6
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• pp.551-558
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• 2006

Effects of Pb and $CO_2$ on soil microbial community associated with Pinus densiflora were investigated using community level physiological profiles (CLPP) and 16S rDNA PCR-denaturing gradient gel electrophoresis (DGGE) methods. Two-years pine trees were planted in Pb-contaminated soils and uncontaminated soils, and cultivated for 3 months in the growth chamber where $CO_2$ concentration was controlled at 380 or 760 ppmv. The structure of microbial community was analyzed in 6 kinds of soil samples (CA-0M : $CO_2$ 380 ppmv + Pb 0 mg/kg + initial, CB-0M : $CO_2$ 380 ppmv + Pb 500 mg/kg + initial, CA-3M : $CO_2$ 380 ppmv + Pb 0 mg/kg + after 3 months, CB-3M : $CO_2$ 380 ppmv + Pb 500 mglkg + after 3 months, EA-3M : $CO_2$ 760 ppmv + Pb 0 mg/kg + after 3 months, EB-3M : $CO_2$ 760 ppmv + Pb 500 mg/kg + after 3 months). After 3 months, the substrate utilization in the uncontaminated soil samples (CA-3M vs EA-3M) was not significantly influenced by $CO_2$ concentrations. However, the substrate utilization in the Pb-contaminated soil samples (CB-3M vs EB-3M) was enhanced by the elevated $CO_2$ concentrations. The results of principal component analysis based on substrate utilization activities showed that the structure of microbial community structure in each soil sample was grouped by Pb-contamination. The similarities of DGGE fingerprints were 56.3 % between the uncontaminated soil samples (CA-3M vs EA-3M), and 71.4% between the Pb-contaminated soil samples (CB-3M vs. EB-3M). The similarities between the soil samples under $CO_2$ 380 ppmv (CA-3M vs CB-3M) and $CO_2$, 760 ppmv (EA-3M vs EB-3M) were 53.3% and 35.8%, respectively. These results suggested that the structure of microbial community associated with Pinus densiflora were sensitively specialized by Pb-contamination rather than $CO_2$ concentration.

• Journal of the Korean Institute of Telematics and Electronics
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• v.26 no.2
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• pp.112-117
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• 1989

This paper describes to the unit rectangle EB direct writing lithography method using SEM. This method has the constant exposure time to any rectangle pattern. In order to change the EB current according to various rectangle size for the constant exposure time, the supply current of condenser lens in controlled by BITMAP-IV CAD system. By this method, the resizing procedure of density pattern area is not needed to pattern data conversion, and the through-put ofr exposure is increased about 172 times compared with the unit scan exposure method.

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.6
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• pp.256-262
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• 2022

The purpose of this study is to define the shear reinforcing effect of Near-Surface-Mounted (NSM) FRP strips in reinforced concrete (RC) member through a test. Three T shaped RC beams were made and two of them were strengthened with NSM FRP strips for increase shear strength. And those were tested to find the shear strengthening effect. In the test, two case of shear strengthening methods were considered such as 1) with NSM FRP strips having full embedded length and 2) with NSM FRP strips having some what short embedded length and additional externally bonded FRP sheet. As a result, the shear strengthening effect could be obtained when the NSM FRP strips are embedded to have full length up to the bottom of slab. However the shear strength was not increased in the case of having somewhat short embedded length of NSM FRP strips even additional EB sheet was enhanced.

• Proceedings of the Korean Information Science Society Conference
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• 2003.04a
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• pp.677-679
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• 2003

e-비즈니스의 활성화에 따른 기업 환경의 변화로 인해 전자상거래와 물류 및 공급망의 현실적/효율적 운영관리를 위한 새로운 개념의 워크플로우 관리 시스템의 필요성이 절대적으로 요구되고 있다. e-비즈니스는 기업 내부 프로세스와 기업간 거래 프로세스와의 연동을 수반하기 때문에 워크플로우 관리 시스템 또한 다중 기업들간의 워크플로우 모델의 상호 연동이 필요하다. 따라서 전자상거래를 위한 기업들은 서로 다른 워크플로우 관리 시스템 및 워크플로우 모델들간의 상호 운영적인 문제를 해결하는 방법에 대한 관심이 증대되었다. 본 논문은, 워크플로우 모델러와 ebXML 기반 비즈니스 프로세스 모델러의 연계를 통해 ebXML을 기반으로 e-비즈니스를 위한 워크플로우 모델링 기법을 제시함으로써, 거래 기업간의 표준화된 비즈니스 프로세스 모델링을 효율적으로 지원하는 것을 목적으로 한다.

• The KIPS Transactions:PartD
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• v.10D no.2
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• pp.351-358
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• 2003

SOAP is a protocol used for information exchange in distributed computing environment and defines mechanisms for message transferring between distributed system and remote procedure cai]/response process. This SOAP is able to be used independently on operating systems and program languages because it is a protocol using text-based XML. Therefore ebXML that is a standard for I-Commerce adopts SOAP for message exchange. In this paper, we developed XML Hub System based on SOAP By this in Hub System we can more easily exchange messages for B2B. Accordingly we can manage and integrate a lots of business partners.

• Journal of Life Science
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• v.22 no.10
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• pp.1407-1414
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• 2012

Hormone replacement therapy (HRT) is an effective regimen that has been found to prevent these diseases in postmenopausal women. However, HRT is accompanied by an increased risk of unfavorable outcomes. This study was conducted to evaluate the effects of Eisenia Bicyclis extract on lipids in ovariectomized rats. Fifty 7-week-old female Sprague-Dawley rats were randomly assigned to four groups: sham-operated rats (SHAM), ovariectomized rats (OVX-CON), and ovariectomized rats that were treated with Eisenia bicyclis extracts. The extract-treated diets were fed to the rats for 6 weeks after operation. Antioxidant effects were measured by DPPH free radical scavenging activity. Antioxidant activity of the ethanol extract increased in a dose-dependent manner and was about 55.9% in a concentration of 100 ${\mu}g/ml$. We measured the total cholesterol content, triglyceride content, HDL-cholesterol content, LDL-cholesterol content, atherosclerotic index, cardiac risk factor in serum, and anti-platelet aggregation and blood rheology. The total cholesterol and triglyceride concentration in serum increased for the OVX-control group, but supplementation with the E. bicyclis extract caused these factors to decrease. Notably, the serum LDL-cholesterol concentration in the OVX-EB200 group was significantly lower than the OVX-CON group. In addition, the blood passage times in rats that received the E. bicyclis extract were more rapid than the times in the untreated group (OVX-CON). Microscopic evaluation revealed that whole blood passed more smoothly through the microchannels in rats in the E. bicyclis extract supplement groups. Our results clarified the effects of E. bicyclis extract on serum lipid content in ovariectomized rats, and consequently we expect positive effects from providing E. bicyclis extract to postmenopausal women with cardiovascular disease.

• Journal of Embryo Transfer
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• v.20 no.3
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• pp.317-322
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• 2005

This study was conducted to improve the efficiency of embryo recovery and to establish the protocols of superovulation in Holstein cows. Sixteen Holstein cows were used the test the efficacy of three superovulation regimens using Folltropin. In the case of regimen 1, CIDR plus with E2 capsule was inserted in cows at the random stage of estrous cycle and the total of 400 mg Folltropin V was adminstered twice a day for 4 days(Folltropin V group). In regimen 2, CIDR was inserted and 3.0 mg estradiol benzoate was administered i.m. next day and the total of 400 mg Folltropin was adminstered twice a day for 4 days(Folltropin V+EB group). For regimen 3, CIDR insertion was same as in the regimen 2 and the total of 400 mg Folltropin diluted with $10\%$ PEG 8,000 was administered once(Folttropin V+PEG 8,000 group). In all the regimens, CIDR were removed on 12th day and 45 mg dinoprost was administered i.m. simultaneously. The heat detected donors were administered 200 ug LH-RH and inseminated twice with 2 straws of frozen semen 12 hours apart. Embryo were collected using Foley catherter in each uterine homs on 6${\~}$8 days after inseminations. The evaluation of collected embryos were according to the IETS manual. The CL responses according to the superovulation treatments were 5.8, 20.6, 24.0 in the Folltropin V, Folltropin+EB and Folltropin V+PE 8,000 groups, respectively and there were significant different among the treatments(p<0.01). Transferable embyos collected were 3.6$\pm$2.4, 3.3$\pm$l.8 and 2.8$\pm$2.3, in the Folltropin V, Folltropin+EB and Folltropin V+PE 8,000 groups, respectively. Degenerated and unfertilized embryos in regimen 2 and 3 than regimen 1. These results indicates that superovulation treatments with both multiple injections and a single injection using PEG of Folltropin combined with CIDR insertion at the random stage of estrus cycle can be used to produce Holstein embryos.

• Information Systems Review
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• v.8 no.1
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• pp.1-24
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• 2006

Now, the Korean footwear industry needs the concrete enhancement of its competitive edge for regaining of its previous well known reputation. For this purpose, this study emphasizes the cooperation between the members through the supply chain of Korean footwear industry, as a c-SCM(Collaborative Supply Chain Management) by aid of information system. The key issue will be how well to coordinate operations flow not only through internal process stages, but also through entire external supply chain stages. In other words, the target goal must be the system optimization through the entire supply chain beyond the local optimization of internal supply chain process. We, at first, analyze the traditional structure of supply chain in Korean footwear industry and find out critical problems, and then, we develop the collaborative information framework in conjunction with several collaborative process modules. The suggested collaborative production & supply planning system was designed for sharing information and it is based on ebXML(electronic business eXtensible Markup Language) framework. In this way, the enhancement of the efficiency and competitiveness can be expected through the synergy effect of coordination of information and material flow, the reduction of lead times, and production costs.

• Proceedings of the Korea Multimedia Society Conference
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• 2002.11b
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• pp.55-58
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• 2002

SOAP(Simple Object Access Protocol)은 분산 환경에서의 정보 교환에 사용되는 분산 컴퓨팅 프로토콜로 분산 시스템간에 메시지를 전달하는 방법과 원격 프로시저 호출/응답을 처리하는 방법 등이 정의되어 있다. 이러한 SOAP는 텍스트 기반 XML을 프로토콜로 사용하고 있기 때문에 하드웨어 플렛폼과 운영체제 및 프로그래밍 언어에 독립적으로 사용할 수 있다. 따라서 전자상거래 표준을 위한 ebXML에서도 메시지 전송을 위해 SOAP을 사용하고 있다. 본 논문에서는 SOAP을 기반으로 XML Hub 시스템을 개발하였다. 이에 따라 본 XML Hub시스템을 기반으로 B2B 메시지 전송이 용이하게 되어, 보다 많은 비즈니스 파트너를 효과적으로 통합 관리할 수 있을 것이다.