• Korean Journal of Environment and Ecology
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• v.32 no.5
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• pp.486-496
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• 2018

In this study, we have selected Larix kaempferi as a study area in Woraksan National Park for understanding the ion index according to the difference of topography in national parks. We measured the weather and ion at two fixed points, ridge and valley, where the Larix kaempferi dominates in the same ecological structure in Woraksan National Park. The weather measurement results showed the average, maximum, and minimum temperatures of $28.22^{\circ}C$, $29.9^{\circ}C$, and $26.4^{\circ}C$, respectively at the ridge. The average, maximum, and minimum temperatures at the valley were $27.08^{\circ}C$, $27.8^{\circ}C$, and $25.5^{\circ}C$, respectively. The average, maximum, and minimum relatively humidities at the ridge were 67.02%, 75.25%, and 61.95%, respectively. he average, maximum, and minimum relatively humidities at the valley were 69.74%, 76.8%, and 63.75%, respectively. The average, maximum, and minimum amounts of positive ions generated in the ridge was $698.40{\pm}59.80ea/cm^3$, $885.88ea/cm^3$, and $597.88ea/cm^3$, respectively. The average, maximum, and minimum amounts of negative ions generated in the ridge were $736.07{\pm}83.89ea/cm^3$, $934.53ea/cm^3$, and $599.32ea/cm^3$, respectively. The ion index is calculated to be 1.06. The average, maximum, and minimum amounts of positive ions generated in the valley were $1,732.49{\pm}354.08ea/cm^3$, $2,652.10ea/cm^3$, and $1,110.92ea/cm^3$, respectively. The average, maximum, and minimum amounts of negative ions generated in the valley were $1,990.47{\pm}433.57ea/cm^3$, $3,126.75ea/cm^3$, and the minimum value was $1,352.17ea/cm^3$. The ion index is calculated to be 1.16. The difference in the amount of negative ions generated in ridge and valley was $1089.26ea/cm^3$, and the difference of the calculated ion index between the ridge portion and the valley portion was 0.10. The results of this study were provided as the reference weather data of national parks for health management.

• Journal of Environmental Science International
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• v.27 no.7
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• pp.489-497
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• 2018

In this study, the ion index characteristics of small and medium urban land use types were investigated in the city of Chungju. The average temperature for each land use type was in the order: general commercial district ($29.59^{\circ}C$) > general residential district ($28.34^{\circ}C$) > productive green district ($28.31^{\circ}C$). The average relative humidity was in the order: productive green district (70.12%) > general residential district (69.93%) > general commercial district (66.48%). The average wind speed was in the order: productive green district (0.95 m/s) > general commercial district (0.87 m/s) > general residential district (0.54 m/s). Positive and negative ions were investigated to determine the ion index by land use type. The average amount of positive ion generated was in the order: general commercial district ($737ea/cm^3$) > general residential district ($492ea/cm^3$) > productive green district ($445ea/cm^3$). The average negative ion production decreased in the order: productive green district ($930ea/cm^3$) > general residential district ($754ea/cm^3$) > general commercial district ($744ea/cm^3$). The ion index calculated from measured data can be arranged in the order: productive green district (2.09) > general residential district (1.53) > general commercial district (1.01). These results confirm the state of positive and negative ion generation in each land use type. Further, the differences in the ion index by land use type were confirmed. However, a limitation of this study is that simple summer measurements were conducted, and seasonal characteristics were not considered. Therefore, any future investigation and research should consider seasonal variation characteristics.

• YAKHAK HOEJI
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• v.43 no.4
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• pp.464-468
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• 1999

In order to find less toxic antiviral agents from basidiomycetes, EA, the water soluble substance, was isolated from the carpophores of Elfvingia applanata (Pers.) Karst. Anti-encephalomyocarditis (EMC) virus activity of EA was examined in Vero cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha and gamma were examined on the multiplication of EMC virus. EA exhibited a concentration-dependent reduction in the plaque formation of EMC virus with 50% effective concentration ($EC_{50}$) of 2.12 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha showed potent synergism with CI values of 0.40~0.60 for 50%, 70% and 90% effective levels, but that with IFN gamma showed antagonism with CI values of 2.16~2.83.

• Journal of Environmental Impact Assessment
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• v.32 no.2
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• pp.146-156
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• 2023

Since the tourism complex development projects is promoted for areas with good natural environment and excellent landscapes, it has s significant environmental impact on the surrounding area. Therefore, this study investigated the current status and various environmental influences of tourist complex based on environmental impact assessment reports from 2012 to 2021. Based on the results of the current status analysis of the development projects, EA-INDEX for the tourism complex development projects was developed and applied to comprehensively and quantitatively analyze various environmental impacts. Through this, we investigated eco-friendliness of the development projects by year in the natural environment conservation sector, resource conservation sector, and living environment protection sector. As a result we found that the tourism complex development projects were carried out in a way that increases eco-friendliness over the past ten years, especially in in the natural environment conservation sector. On the other hand, in the case of resource conservation, it has been confirmed that eco-friendliness is decreasing, so it is necessary to make efforts to improve it when establishing new development plans in the future. It is expected that the result of this study will be useful for identifying the development trend and environmental impact of development projects. If an analysis is made that synthesizes information such as policy events and social issues related to development projects in the future, it is also expected that a broader explanation will be possible to identify trends in development projects using EA-INDEX.

• Natural Product Sciences
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• v.5 no.2
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• pp.107-111
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• 1999

To investigate less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was isolated from the carpophores of Elfvingia applanata (pers.) Karst. Anti-varicella zoster virus (Oka strain; anti-VZV/Oka) activity of EA was examined in MRC-5 cells by plaque reduction assay in vitro. And the combined antiviral effects of EA with nucleoside anti-VZV agents, acyclovir and vidarabine, were examined on the multiplication of VZV/Oka. EA exhibited a concentration-dependent reduction in the plaque formation of VZV/Oka with a 50% effective concentration $(EC_{50})$ of $464.14\;{\mu}g/ml$. The results of combination assay were evaluated by the combination index (CI) that was calculated by the multiple drug effect analysis. The combination of EA with acyclovir showed more potent synergism with CI values of $0.18{\sim}0.62$ for $50{\sim}90%$ effective levels than that of EA with vidarabine with CI values of $0.67{\sim}1.04$.

• YAKHAK HOEJI
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• v.38 no.6
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• pp.742-748
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• 1994

As part of our search for less toxic antimicrobial agents from natural resources, the carpophores of Elfvingia applanata$(P_{ers}.)K_{ARST}.$ was extracted with hot water. EA, the aqueous extract from the carpophores of E. applanata, was lyophilized and a dark brownish powder was obtained. Antimicrobial activity of EA was tested in vitro against Gram positive and Gram negative bacteria by serial broth dilution method, and the antimicrobial activity was expressed by minimal inhibitory concentration(MIC). Among fourteen species of bacteria tested, the antimicrobial activity of EA was the most potent against Proteus vulgaris showing MIC of 1.250 mg/ml. To investigate the effect of antimicrobial combinations of EA with four kinds of antibiotics(ampicillin, cefazolin, oxytetracycline and chloramphenicol), the fractional inhibitory concentration index(FICI) was determined by checkerboard assay for each strain. The antimicrobial combinations of EA with four kinds of antibiotics resulted in synergism in four instances, but no antagonism was observed. Four instances of synergism were observed when EA was combined with ampicillin against Micrococcus luteus, with cefazolin against Bacillus subtilis, with cefazolin against Micrococcus luteus and with oxytetracycline against Staphylococcus aureus.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.25-33
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• 1999

In order to find less toxic antiviral agents from basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. EA was examined for antiviral activity against five strains of pathogenic viruses such as encephalomyocarditis virus (EMCV), vesicular stomatitis virus (VSV) Indiana and New Jersey strains, influenza A virus (Flu A), and varicella zoster virus (VZV) in vitro. Antiviral activity was evaluated by plaque reduction assay. Among five strains of viruses tested, EA exhibited the most potent antiviral activity against VSV Indiana strain with 50% effective concentration $(EC_{50})$ of 0.104 mg/ml in Vero cells, and its selectivity index (SI) was 36.5. EA was also examined for the virucidal activity, antiviral activity in preincubation on VSV Indiana strain in order to examine possible mode of antiviral activity. Preincubation of Vero cells with EA did not confer protection against VSV, however, prolonged exposure of cells to EA inhibited the replication of virus dose-dependently. In virucidal activity, the titer of infectious virus did not decrease significantly.

• Journal of Dairy Science and Biotechnology
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• v.36 no.2
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• pp.121-124
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• 2018

Ellagic acid (EA) is a naturally occurring polyphenolic compound in vegetables, nuts, and fruits such as berries. EA has antioxidant, anticancer, anti-allergy, and anti-inflammatory activities. The objectives of this research were to investigate the physicochemical properties of nanoparticles before and after nano-encapsulation of EA in dairy protein and to develop a functional (anti-inflammatory) dairy protein-based beverage containing EA. A particle size analyzer was used to determine the physicochemical and morphological properties. High performance liquid chromatography was used to evaluate the entrapment efficiency of EA. The nanoparticles containing EA were 100 to 200 nm in diameter. The determined poly dispersity index value of 0.3 to 0.4 indicated that the nanoparticles were uniformly distributed with similar size. Zeta-potential values were also similar between the control groups. The entrapment efficiency of EA was nearly 90%. The results indicate the potential for development of nanoparticles containing EA beverage products with anti-inflammatory activity.

• YAKHAK HOEJI
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• v.43 no.4
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• pp.469-473
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• 1999

EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers). Karst. Anti-influenza A virus (anti-Flu A) activity of EA was examined of Vero cells by plaque reduction assay in vitro. And the combined antiviral effects fo EA with interferon (IFN) alpha and gamma were examined on the multiplication of Flu A with 50% effective concentration ($EC_50$) of 1.50 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha on Flu A showed more potent synergism with CI values of 0.50~0.52 of 50%, 70%, 90% effective levels than that with IFN gamma with CI values of 0.82~0.99.

• The Korean Journal of Mycology
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• v.27 no.2 s.89
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• pp.175-179
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• 1999

In order to find less toxic antiviral agents from Basidiomycetes, EA, the water soluble substance, was prepared from the carpophores of Elfvingia applanata (Pers.) Karst. Antiviral activity of EA against vesicular stomatitis virus [New Jersey serotype, VSV(NJ)] was examined in Vero cells using plaque reduction assay in vitro. And the combined antiviral effects of EA with interferon (IFN) alpha and gamma were examined on the multiplication of VSV(NJ). EA caused a concentration-dependent reduction in the plaque formation of VSV(NJ) with 50% effective concentration $(EC_{50})$ of 2.10 mg/ml. The results of combination assay were evaluated by the combination index (CI) that was analysed by the multiple drug effect analysis. The combination of EA with IFN alpha showed more potent effect with CI values of $0.87{\sim}1.59$ for 50%, 70% and 90% effective levels than that with INF gamma with CI values of $1.05{\sim}2.03$.