• Title/Summary/Keyword: E1

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Shielding Analysis for Industrial Package: Focusing on Dry Active Waste (IP형 운반용기 차폐해석-잡고체폐기물을 중심으로)

  • Lee Kang-Wook;Cho Chun-Hyung;Jang Hyun-Kie;Choi Byung-Il;Lee Heung-Young
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2005.06a
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    • pp.523-530
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    • 2005
  • In this study, maximum exposure rate at DAW(Dry Active Waste) drum surface which is satisfying regulation limit was calculated for conceptual design of IP(Industrial Package). DAW can be classified as combustible and non-combustible waste and the calculation was conducted for single and mixed radionuclide for each type of waste. In case of combustible waste that mixed radionuclide is uniformly distributed, the maximum exposure rates at drum surface were 3.60E-01, 8.85E-01 and 1.27E+01 mSv/hr for IP Type 1, 2-a and 2-b, respectively. and 3.60E-01, 8.85E-01, 1.27E+01 mSv/hr for single radionuclide(Co-60). In case of non-combustible waste that mixed radionuclide is uniformly distributed, the maximum exposure rates at drum surface were 7.14E-01, 1.83E+00, 2.69E+01 mSv/hr for IP Type 1, 2-a and 2-b, respectively. and 7.13E-01, 1.81E-01, 2.62E+01 mSv/hr for single radionuclide(Co-60). Through this study, the maximum amount of DAW can be transported by IP was suggested as maximum exposure rate at drum surface and the calculation for the other types of waste will be conducted.

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유기용매에 의한 CYP2E1의 유도발현 : 단백질형성 효율의 증가에 따른 조절규제기전

  • ;Raymond F. Novak
    • Toxicological Research
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    • v.9 no.2
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    • pp.153-158
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    • 1993
  • Pyridine and acetone are efficacious inducers of CYP2E1 in both rats and rabbits. The response in the elevation of CYP2E1 levels, changes in CYP2E1 mRNA levels, and enhanced translational processing of hepatic CYP2E1 mRNA during the early phase of CYP2E1 induction by the solvents pyridine and acetone were examined. Time-depen-dent incease in CYP2E1 levels occurred at early times (6-24h) following a single dose of pyridine treatmene, as assessed by Western immunoblot analysis, whereas the levels in CYP2E1 mRNA transiently decreased at 12h post-treatment, returning to the level present in untreated animals.

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MC1R Genotypes, Coat Color, and Muzzle Phenotype Variation in Korean Native Brindle Cattle (MC1R 유전자의 유전자형과 칡소의 모색 발현 및 비경색 분포에 관한 연구)

  • Park, Jae-Hee;Lee, Hae-Lee;Kim, Yong-Su;Kim, Jong-Gug
    • Journal of Animal Science and Technology
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    • v.54 no.4
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    • pp.255-265
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    • 2012
  • The objectives of this study were to investigate MC1R genotype, coat color, and muzzle phenotype variationsin the Korean native brindle cattle (KNBC) maintaining family lines and to establish the mating system for increased brindle coat color appearance. KNBC with genotype and phenotype records were selected as experimental animals. The relationship between melanocortin 1 receptor (MC1R) genotypes, verified by PCR-RFLP, and brindle coat color appearance was determined. Fragments of the MC1R gene amplified by PCR were digested with MspI and RFLP was determined. KNBC had $E^+E^+$, $E^+e$, and ee genotypes. The $E^+e$ genotype was most common with 65%, compared to $E^+E^+$ (33.33%), or ee (1.67%). When the sire had $E^+e$ genotype and the dam had $E^+E^+$ genotype, and both of them had the whole body-brindle coat color, all of their offspring (4/4) had whole body-brindle coat color. When the sire had $E^+E^+$ genotype and the dam had $E^+e$ genotype, and both had whole body-brindle coat color, 44.44% (4/9) of the offspring had whole body-brindle coat color. The mating between the sires and dams with these two genotypes with whole body-brindle coat color may have the highest whole body-brindle coat color appearance in their offspring. Muzzle grades 3 or 4 were more common than other muzzle grades. This is the first report indicating the segregation of MC1R genotypes and the inheritance of coat color through family lines in KNBC. The mating system proposed from this study may increase the possibility of brindle coat color appearance in KNBC.

E1B-19k does not Localize in Mitochondria nor Dimerize Bax even with the Staurosporine (E1B-19k의 세포내 위치와 Bax와의 Dimerization에 관한 연구)

  • Yoon, Soo Han;Kim, Jin Young;Park, Seung Woo;Ahn, Young Hwan;Ahn, Young Min;Cho, Ki Hong;Cho, Kyung Gi
    • Journal of Korean Neurosurgical Society
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    • v.29 no.6
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    • pp.725-730
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    • 2000
  • Purpose : The subcellular localization of E1B-19k has been known cytosol or nuclear membrane by immunohistochemical staining and could dimerize with Bax to regulate cell death also known by the in-vitro immunoprecipitation. We planed to confirm this dimerization of E1B-19k with Bax in vivo in Cos-7 cells by using green fluorescent protein. Material and Method : We cloned E1B-19k and Bax into C3-EGFP. C3-EGFP-E1B-19k, C3-EGFP-Bax, and C3-EGFP-E1B-19k and pcDNA3-Bax were transfected into Cos-7 cells. We explored location of E1B-19k and Bax, and confirmed its dimerization with Bax in transfected living healthy Cos-7 cells by following green fluorescent protein of E1B-19k on the confocal microscope. Results : E1B-19k was located diffusely in cytoplasm and in nucleus but not in mitochondria. It prevented cell death from the apoptosis by staurosporine but its location was not changed. GFP-E1B-19k is not changed its intracellular location with Bax even with staurosporine. Conclusion : These results support that E1B-19k does not localize in mitochondria nor dimerize with Bax even with staurosporine. We could anticipate E1B-19k prevent cell death via the other dimerizing partner or pathways.

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AN ACTION OF A GALOIS GROUP ON A TENSOR PRODUCT

  • Hwang, Yoon-Sung
    • Communications of the Korean Mathematical Society
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    • v.20 no.4
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    • pp.645-648
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    • 2005
  • Let K be a Galois extension of a field F with G = Gal(K/F). Let L be an extension of F such that $K\;{\otimes}_F\;L\;=\; N_1\;{\oplus}N_2\;{\oplus}{\cdots}{\oplus}N_k$ with corresponding primitive idempotents $e_1,\;e_2,{\cdots},e_k$, where Ni's are fields. Then G acts on $\{e_1,\;e_2,{\cdots},e_k\}$ transitively and $Gal(N_1/K)\;{\cong}\;\{\sigma\;{\in}\;G\;/\;{\sigma}(e_1)\;=\;e_1\}$. And, let R be a commutative F-algebra, and let P be a prime ideal of R. Let T = $K\;{\otimes}_F\;R$, and suppose there are only finitely many prime ideals $Q_1,\;Q_2,{\cdots},Q_k$ of T with $Q_i\;{\cap}\;R\;=\;P$. Then G acts transitively on $\{Q_1,\;Q_2,{\cdots},Q_k\},\;and\;Gal(qf(T/Q_1)/qf(R/P))\;{\cong}\;\{\sigma{\in}\;G/\;{\sigma}-(Q_1)\;=\;Q_1\}$ where qf($T/Q_1$) is the quotient field of $T/Q_1$.

Kinemetic analysis of a thumping security motion with an expandable barton (경호원의 삼단봉 머리치기 동작의 운동학적 분석)

  • Kim, Yong-Hak;Kim, Sin-Hye;Jung, Sung-Bae
    • Korean Security Journal
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    • no.36
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    • pp.93-109
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    • 2013
  • This research is mainly based on the experimental result due to seek different outcomes whena certain security motion with a paticular gear is applied in a plausible confrontational situation. For the purpose of this research an Expandable Baton, which is one of the most commonsecurity equipments, was chosen to be applied in a situation of hitting a person's head. Alsothe results will be studied in the view of Kinematic theory. To demonstrate, 10 students who were majeored in Escort Crane studies at 'H' university werechosen as testees. The participants were grouped into two-one is practiced with the 'expanadable baton use program' and the other is pre-practiced. In this report two groups abovewill be reffered as 'group A' and 'group B' for conveniency. There were a number of differences and changes between two groups. Group B took more timethan the other group did. Group A spent about 0.428sec in section 'e2' and 0.230sec in section'e3' while Group B took 0.435sec, 0.232sec in each sections.To add on, more distinctive results were out when it was more focused on physical movements. Two gropus presented considerable changes- in an 'left-right' moving displacement-Group A;$2.16{\pm}0.9cm$ (left side), $3.78{\pm}1.42cm$ (right side), total $5.94{\pm}2.03cm$. Group B; $2.97{\pm}1.01cm$ (left side),$4.56{\pm}1.57cm$ (right side), total $7.53{\pm}2.13cm$.Continuously, different outcomeswere shown between two groups in a 'back and forth' moving displacement-Group A;$32.48{\pm}3.86cm$, $35.21{\pm}4.64cm$, total $69.36{\pm}5.72$. Group B; $34.50{\pm}6.12cm$, $37.04{\pm}3.70cm$, total $71.46{\pm}7.17cm$. Furthermore, changes in an 'up and down' moving displacement were - GroupA; $5.62{\pm}2.41cm$, $4.54{\pm}1.87cm$, total $10.11{\pm}1.57cm$. Group B; $6.33{\pm}1.78cm$, $4.86{\pm}1.85cm$,total $10.68{\pm}1.81cm$. To continue, there were few modifications of degree on participants' joints, espcially on 'Wristjoint', 'Elbow joint' and 'Shoulder joint', depend on different sections -Wrist joint;Group A; e1 $114.62{\pm}7.13$, e2 $68.27{\pm}6.37$, e3 $131.64{\pm}6.27$. Group B; e1 $112.62{\pm}6.13$, e2 $66.28{\pm}7.38$, e3$137.42{\pm}4.28$ and Elbow joint ; Group A e1 $132.31{\pm}6.55$, e2 $117.92{\pm}8.42$, e3 $144.41{\pm}6.32$. Group B; e1 $133.58{\pm}8.56$, e2 $114.45{\pm}8.21$, e3 $139.89{\pm}4.38$. Lastly, degree changes ofshoulder joint were; Group A; e1 $13.55{\pm}3.85$, e2 $131.42{\pm}11.24$, e3 $78.32{\pm}6.28$. Group B; e1$9.45{\pm}1.23$, e2 $136.74{\pm}13.21$, e3 $79.75{\pm}4.24$.

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EVALUATION AS A BIOASSAY PREPARATION OF TORTOISE INTESTINE FOR PROSTAGLANDIN $E_1$

  • Hong, Ki-W.
    • The Korean Journal of Pharmacology
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    • v.11 no.2
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    • pp.41-46
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    • 1975
  • The isolated strips of tortoise intestine are evaluated as a test organ for bioassay of prostaglandin $E_1$. This preparation responded highly sensitively to $PGE_1$ and $PGE_2$ in picogram concentration range. The mean slope and the value of precision index among the doses of 0.1, 0.3 and 0.5ng/ml in final concentration were 37.7 and 0.143, respectively. And this was relatively insensitive to different prostaglandins; $E_1/E_2{\gtrsim}1$, $E_1/A_2{\sim}50$ and $E_1/F_{2\alpha}{\sim}100$, and showed the dual responses to 5-hydroxytryptamine and histamine; initial contraction followed by relaxation. The dose-ratio inducing the relative equal contraction height for $PGE_1$, acetylcholine, caerulein, angiotensin and barium chloride was 0.4 : 50 : 25 : 10 : 100 in this order. These results suggest that the intestinal strips of the tortoise are suitable for bioassay of prostaglandin $E_1$ and $E_2$ between the doses of 0.1 and 1.0 ng/ml level in the tissue extracts.

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Transcriptional activation of pref-1 by E2F1 in 3T3 L1 cells

  • Shen, Yan-Nan;Kim, Yoon-Mo;Yun, Cheol-Heui;Moon, Yang-Soo;Kim, Sang-Hoon
    • BMB Reports
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    • v.42 no.10
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    • pp.691-696
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    • 2009
  • The E2F gene family appears to regulate the proliferation and differentiation of events that are required for adipogenesis. Pref-1 is a transmembrane protein that inhibits adipocyte differentiation in 3T3-L1 cells. In this study, we found that the expression of pref-1 is regulated by the transcription factor E2F1. The expression of pref-1 and E2F1 was strongly induced in preadipocytes and at the late differentiation stage. Using luciferase reporter assay, ChIP assay and EMSA, we found that the -211/-194 region of the pref-1 promoter is essential for the binding of E2F1 as well as E2F1-dependent transcriptional activation. Knockdown of E2F1 reduced both pref-1 promoter activity and the level of pref-1 mRNA. Taken together, our data suggest that transcriptional activation of pref-1 is stimulated by E2F1 protein in adipocytes.

Deep Learning Based Digital Staining Method in Fourier Ptychographic Microscopy Image (Fourier Ptychographic Microscopy 영상에서의 딥러닝 기반 디지털 염색 방법 연구)

  • Seok-Min Hwang;Dong-Bum Kim;Yu-Jeong Kim;Yeo-Rin Kim;Jong-Ha Lee
    • Journal of the Institute of Convergence Signal Processing
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    • v.23 no.2
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    • pp.97-106
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    • 2022
  • In this study, H&E staining is necessary to distinguish cells. However, dyeing directly requires a lot of money and time. The purpose is to convert the phase image of unstained cells to the amplitude image of stained cells. Image data taken with FPM was created with Phase image and Amplitude image using Matlab's parameters. Through normalization, a visually identifiable image was obtained. Through normalization, a visually distinguishable image was obtained. Using the GAN algorithm, a Fake Amplitude image similar to the Real Amplitude image was created based on the Phase image, and cells were distinguished by objectification using MASK R-CNN with the Fake Amplitude image As a result of the study, D loss max is 3.3e-1, min is 6.8e-2, G loss max is 6.9e-2, min is 2.9e-2, A loss max is 5.8e-1, min is 1.2e-1, Mask R-CNN max is 1.9e0, and min is 3.2e-1.

Microsecond molecular dynamics simulations revealed the inhibitory potency of amiloride analogs against SARS-CoV-2 E viroporin

  • Jaber, Abdullah All;Chowdhury, Zeshan Mahmud;Bhattacharjee, Arittra;Mourin, Muntahi;Keya, Chaman Ara;Bhuyan, Zaied Ahmed
    • Genomics & Informatics
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    • v.19 no.4
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    • pp.48.1-48.10
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    • 2021
  • Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) encodes small envelope protein (E) that plays a major role in viral assembly, release, pathogenesis, and host inflammation. Previous studies demonstrated that pyrazine ring containing amiloride analogs inhibit this protein in different types of coronavirus including SARS-CoV-1 small envelope protein E (SARS-CoV-1 E). SARS-CoV-1 E has 93.42% sequence identity with SARS-CoV-2 E and shared a conserved domain NS3/small envelope protein (NS3_envE). Amiloride analog hexamethylene amiloride (HMA) can inhibit SARS-CoV-1 E. Therefore, we performed molecular docking and dynamics simulations to explore whether amiloride analogs are effective in inhibiting SARS-CoV-2 E. To do so, SARS-CoV-1 E and SARS-CoV-2 E proteins were taken as receptors while HMA and 3-amino-5-(azepan-1-yl)-N-(diaminomethylidene)-6-pyrimidin-5-ylpyrazine-2-carboxamide (3A5NP2C) were selected as ligands. Molecular docking simulation showed higher binding affinity scores of HMA and 3A5NP2C for SARS-CoV-2 E than SARS-CoV-1 E. Moreover, HMA and 3A5NP2C engaged more amino acids in SARS-CoV-2 E. Molecular dynamics simulation for 1 ㎲ (1,000 ns) revealed that these ligands could alter the native structure of the proteins and their flexibility. Our study suggests that suitable amiloride analogs might yield a prospective drug against coronavirus disease 2019.