• Korean Journal of Food Science and Technology
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• v.39 no.6
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• pp.614-618
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• 2007

The effect of irradiation on the quality characteristics of lyophilized kimchi powder was investigated in order to develop a commercial kimchi seasoning. Fresh and fermented kimchi powders were irradiated at 0, 1.5, 5, 10 and 30 kGy using a Linear Accelerator. By increasing the irradiation dose level, $a^*$ (redness) and $b^*$ (yellowness) values of the kimchi powders were decreased, while $L^*$ (lightness) value remained relatively unchanged as compared to the control. As the main volatile compounds, butanal, 2-butanone and acetic acid were produced in both of the kimchi powders at 30 kGy and dipropyl disulfide was detected only in the fermented kimchi. The viable counts of aerobic bacteria, yeasts, molds, and lactic acid bacteria in the kimchi powder were significantly reduced by all irradiation doses. However, aerobic and lactic acid bacteria were still observed in both of the kimchi powders at 30 kGy. No significant off-odors or off-tastes were produced in either of the kimchi powders by irradiation, while pungency decreased after irradiation. These results suggest that irradiated kimchi powder could be used as a kimchi seasoning.

• Koreanishche Zeitschrift fur Deutsche Sprachwissenschaft
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• v.7
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• pp.237-256
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• 2003

Das Deutsche als Fremdsprach hat sich als akademische Disziplin etabliert; es hat seine - von vielerlei Irrungen und Wirrungen begleitete - Startphase hinter sich und ist in eine Phase der Konsolidierung eingetreten. Bei aller Unterschiedlichkeit der $Ans\"{a}tze$, Forschungsmethoden und Lemziele gibt es doch eine Einheit in der Vielfalt, was angesichts der erst wenige ]ahrzehnte alten Disziplin erstaunt. Im Zentrum stehen nach allgemeiner Auffassung die Theorie und Praxis des Lehrens und Lernens/ Erwerbens des Deutschen als Fremdsprache; unterschidliche Auffassungen bestehen vor allem daruber, ob Deutsch als Fremdsprache ein germanistisches oder aber ein fremdsprachenphilologisches Fach sei. Unbestritten sind dagegen Fremdperspektive und $Interdisziplinarit\'{a}t$ der Disziplin rnit zahlreichen Beztigen zu Nachbarwissenschaften. Eine wesentliche Rolle in der Diskussion spielte stets die Rolle der Grammatik im fremdsprachlichen Deutschunterricht. Freilich hat die Diskussion ergeben, dass dieses in Wahrheit eine Scheinaltemative ist, die auf einer Reihe falscher Annahmen oder $Missverst\"{a}ndnissen$ beruht, vor allem jenen, im Unterricht komme es $ausschlie{\ss}lich$oder zurnindest vorrangig auf Fertigkeiten und Kommunikation an, nicht oder nur am Rande auf die Vermittlung sprachsystematischer Erkenntnisse. Durchgesetzt hat sich heute freilich die Erkenntnis, dass Grammatik, verstanden in diesem weiten Begriffssinn, aus dem Fremdsprachenunterricht nicht zu verbannen ist. Weiterhin $d\"{u}rfte$ Einvemehmen dariiber herrschen, dass Grammatik und Kommunikation keinen Gegensatz darstellen, vielmehr das Lernziel kommunikative Kompetenz die Beherrschung grammatischer Regeln einbezieht, wenn nicht voraussetzt. Der Funktionsbegriff ist umstritten. Der Oberbegriffe $f\"{u}r$ alle Funktionen $w\"{a}re$ Kommunikation. Fur unser Verstandnis einer funktionalen Grammatik fur Lemende des Deutschen als Fremsprache ist die entscheidende Frage, welche Funktion in der Kommunikation eine bestimmte Struktur spielt. Das Ziel oder der Zweck der kommunikativen Handlung steht im Mittelpunkt. Wir meinen, dass auch jenseits des deutschen Sprachraums Form und Funktion des sprachlichen Zeichens eine Einheit darstellen und im Unterricht nicht getrennt werden $k\"{o}nnen$. Zuerst lernen wir die Formen und Strukturen sprachlicher Mittel kennen, und danach werden wir sie praktisch an: Regelwissen, aber Versagen in der Kommunikation. Die Beispiele der sprachlichen $M\"{o}glichkeiten$ der Satzarten unter funktionalem Aspekt und des Ausdrucks der $Modalit\"{a}t$ im Deutschen machen die Notwendigkeit eines funktional-kommunikativen Ansatzes einer Grammatik fur Deutsch als Fremdsprache deutlich. Es belegt zugleich, dass - unter dem Primat des Didaktischen - die Grenze von Grammatik und Lexikon $\"{u}berwinden$ werden muss, um den Lemenden wirkliche $Erkl\"{a}rung$ und Lernhilfen zu bieten.

• Journal of rehabilitation welfare engineering & assistive technology
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• v.9 no.2
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• pp.89-103
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• 2015

The purpose of this research attempted to developed the Community Reintegration support Program(H-CRsP) on patients with Spinal Cord Injury(SCI) and identity the effect. To assess the effectiveness of a Hospital based H-CRsP with a comprehensive team approach for treating inpatients with Spinal Cord Injury(SCI), offered by the Korea National Rehabilitation Hospital. Participants with SCI were recruited from the Korean National Rehabilitation Hospital. Twenty-five participants who met inclusion criteria were provided with a H-CRsP. A H-CRsP with modules related to the training of daily living skills, driving, vocations and school support activities, leisure or recreational activities, peer counseling, sexual rehabilitation, and others support activities. The 25 enrolled patients had been treated with average of 6.1 programs 2 times a week for over 2 or 3months. COPM, HADS, WHOQOL-BREF, AD-R were administered before the treatment(pre-test) and also at the time of discharge(post-test) to compare the treatment outcomes in the 25 program participants. Measured level of community integration by CIQ at discharge. The data were analyzed by such statistical methods as frequency and paired t-test analysis. Significant improvements were found on the Occupational Performance and Satisfaction, Acceptance of Disability, Quality of Life score in the COPM, AD-R, WHOQOL-BREF (p<0.05). In addition, 10 of the 25 patients returned to home, work or school. These results demonstrated the effectiveness of the H-CRsP in helping patients with SCI return their communities to which they belong. It considered development of the variety of programs and prepare systematically related system.

• Journal of Haehwa Medicine
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• v.4 no.2
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• pp.335-336
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• 1996

Artemisinin, a new antimalarial to treat patients infected with strains of Plasmodium jalciparum, derived from the plant Artemisia annua Linn, has immunopharmacologic actions such as enhence the PHA -induced lymphocyte transformation rate, increased the weight of spleen but reduced the weight of thymus, reduced phagocytic function of peritoneal macrophage, remarkably reduced the level of serum IgG and hemolysin fonning capacity (sentitized with SRBC), inhibited the activity of Ts cells of donor mice by supraoptimal immunuization(SOI), but enhenced activity of Ts cells of recipient mice by SOI. These results suggested that Ts cells may be the target cells of artemisinin. To the serum complement C3 level of plasmodium berghei-infeted mice, artemisinin (i. m,) could remarkly increase it. The artemisinin also obviously reduced the prostaglandin E(PGE) in the mouse hind paw swelling induced by carrageenin. Numerous studies have demonstrated that pharmacologic doses of PGE attenuate the development of immunocomplex nephritis. Some autologous immune mechanisms may be invoolved In the pathogensis of some types of glomurulonephritis. Glomerular abnormalities can be induced in animals by variety of immunological manipulations. The resulting disorder has many clinical and pathogical similarities to the disease in human. Our purpose was therefore to test the ability of the artemisinin to lessen the severity of rabbit IgG accelerated nephrotoxic serum glomerulonephritis in mice model. Mice which had treated with rabbit IgG and NTS, administrated with saline, showed Significant inceases of urinary protein, cholesterol level, and decrease of serum albumin in NS group. On the contrary, By i.g. adminstration of artemisinin at dose of 12.5, 25 and 50 mg/kg for 14 days after NTS injection, shown that artemisinin inhibited the nephritic changes in some parameters by means of urinary protein(p<0.05, p<0.01) and serum choleterol(p<0.05, p<0.01) and albumin (p<0.05, p<0.01), blood urea nitrogen (p<0.05, p<0.01), serum albumin(p<0.05, p<0.01); Cyclophosphamide(i.p. 10mg/kg for 14d) had almost same effect as the artemisinin had. Morphological studies shown that The picture of kidney from the mouse with NTS-nephritis accerated with rabbit IgG, treated with i.g. saline as the control, the mesangiocapillary were enlarged and proliferated; There were inflammatory cells infiltrating around the glomeruli; The ethelial cell were proliferated in the wall of Bowman's capsule. Histopatholological picture of kidney from the NTS-nephritis accerated with rabbit IgG mouse treated with i.p. 10mg/kg cyclophosphamide as the positive control. No siginicant histopathological evidence were found. Treaded with i.p. 12.5mg/kg artemisinine, the picture shown that mesangiocapillary were lightly proliferated; There were inflammatory cells infiltrating around the glomeruli; Treaded with i.p. 25mg/kg artemisinine, The picture shown that the mesangiocapillary were lightly proliferated; Treaded with i.p. 50mg/kg artemisinine, The picture shown that both the mesangiocapillary proliferated and the inflammatory cells infiltrating around the glomeruli are less than treated with saline, 12.5 and 25 mg/kg artemisinine. On the basis of these studies we conclude that the artemisinin can relieve pathological change caused by NTS-nephritis aacerated with rabbit IgG.

• Korean Journal of Weed Science
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• v.15 no.2
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• pp.121-130
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• 1995

Greenhouse and laboratory studies were conducted to investigate allelopathic potential of some weed species on alfalfa(Medicago sativa L.) germination and seedling growth. In the comparison between top(leaves+stems) and root extracts, top extract exhibited greater allelopathic effects on alfalfa germination than that of root. The various weed species extract differently responded to alfalfa test species, WL-320, in terms of allelopathic effect. Top and root aqueous extracts of lambsquarter(Chenopodium album L.), giant foxtail(Setaria faberii Herrm.), redroot pigweed (Amaranthus retroflexus L.), velvetleaf(Abutilon theophrasti Medic.), crabgrass(Digitaria sanguinalis L.), canada thistle(Cirsium arvense L.) and prostrate knotweed(Polygonium aviculare L.) significantly inhibited germination, seedling length, weight, vigor, and rate of germination of alfalfa. The regression slopes of various top extracts showed that velvetleaf(b=3.69) extracts were the most inhibitory, while large crabgrass(b=2.39) extracts had the least allelopathic effect on alfalfa germination. Germination, seedling length and weight of alfalfa were inversely proportional to the concentration of dried velvetleaf extracts. Also, more of the toxic effects were observed from the dried extracts compared to the fresh extracts. Residue of velvetleaf inhibited significantly alfalfa emergence and survival percentage compared to the control. The emergence and survival percentage of alfalfa were 44%, 57% at 1.0% residue treatment, respectively. When weed residues were mixed with silica sand with incubation time, velvetleaf residue most inhibited alfalfa growth. The degree of inhibition increased as incubation time increased. An incubation for 72h caused the greatest inhibition of alfalfa growth. These results demonstrate the different allelopathic activity of weed species extracts on alfalfa and suggest that weed may affect alfalfa growth and development through the inhibitory effects of allelochemicals present in weed tissue.

• Molecules and Cells
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• v.40 no.2
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• pp.109-116
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• 2017

Soybean agglutinin (SBA) is an anti-nutritional factor of soybean, affecting cell proliferation and inducing cytotoxicity. Integrins are transmembrane receptors, mediating a variety of cell biological processes. This research aims to study the effects of SBA on cell proliferation and cell cycle progression of the intestinal epithelial cell line from piglets (IPEC-J2), to identify the integrin subunits especially expressed in IPEC-J2s, and to analyze the functions of these integrins on IPEC-J2 cell cycle progression and SBA-induced IPEC-J2 cell cycle alteration. The results showed that SBA lowered cell proliferation rate as the cell cycle progression from G0/G1 to S phase (P < 0.05) was inhibited. Moreover, SBA lowered mRNA expression of cell cycle-related gene CDK4, Cyclin E and Cyclin D1 (P < 0.05). We successfully identified integrins ${\alpha}2$, ${\alpha}3$, ${\alpha}6$, ${\beta}1$, and ${\beta}4$ in IPEC-J2s. These five subunits were crucial to maintain normal cell proliferation and cell cycle progression in IPEC-J2s. Restrain of either these five subunits by their inhibitors, lowered cell proliferation rate, and arrested the cells at G0/G1 phase of cell cycle (P < 0.05). Further analysis indicated that integrin ${\alpha}2$, ${\alpha}6$, and ${\beta}1$ were involved in the blocking of G0/G1 phase induced by SBA. In conclusion, these results suggested that SBA lowered the IPEC-J2 cell proliferation rate through the perturbation of cell cycle progression. Furthermore, integrins were important for IPEC-J2 cell cycle progression, and they were involved in the process of SBA-induced cell cycle progression alteration, which provide a basis for further revealing SBA anti-proliferation and anti-nutritional mechanism.

• Journal of Embryo Transfer
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• v.32 no.3
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• pp.87-94
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• 2017

Tissue engineering (TE) has been developed to create functional organs and tissue by combining 3D matrix and cells in vitro. Vascularization and angiogenesis are utmost important for supply of nutrients and oxygen in tissue engineered organs. The present study was performed to isolate and characterize primary endothelial cells (EC) from aorta of alpha 1, 3-enzyme galactosyltransferase knock out (GalT KO) pig, to minimize immune rejection and analyze body immune system for future xenotransplantation studies. Isolation of primary EC from aorta were performed by incubation with dispase for 8-10 min at $37^{\circ}C$. Primary EC were cultured in EC growth medium on different extra cellular matrix (ECM), either collagen or gelation. Primary EC exhibits morphological characteristics and showed positive expressions of EC specific marker proteins i.e. PECAM1, KDR and VWF despite of their ECM surface; however, on collagen based surface they showed increase in mRNA level analyzed by qPCR. Primary EC cultured on collagen were sorted by flow cytometer using KDR marker and cultured as KDR positive cells and KDR negative cells, respectively. KDR positive cells showed dramatically increased in PECAM1 and VWF level as compared to KDR negative cells. Based on the above results, primary EC derived from GalT KO are successfully isolated and survived continuously in culture without becoming overgrown by fibroblast. Therefore, they can be utilize for xeno organ transfer, tissue engineering, and immune rejection study in future.

• Journal of Climate Change Research
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• v.6 no.4
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• pp.357-366
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• 2015

This study was conducted to estimate carbon stocks of Quercus serrata with drawing volume of trees in each tree height and DBH applying the suitable stem taper equation and tree specific carbon emission factors, using collected growth data from all over the country. Information on distribution area, tree number per hectare, tree volume and volume stocks were obtained from the $5^{th}$ National Forest Inventory (2006~2010), and method provided in IPCC GPG was applied to estimate carbon storage and removals. Performance in predicting stem diameter at a specific point along a stem in Quercus serrata by applying Kozak's model,$d=a_1DBH^{a_2}a_3^{DBH}X^{b_1Z^2+b_2ln(Z+0.001)+b_3{\sqrt{Z}}+b_4e^Z+b_5({\frac{DBH}{H}})}$, which is well known equation in stem taper estimation, was evaluated with validations statistics, Fitness Index, Bias and Standard Error of Bias. Consequently, Kozak's model turned out to be suitable in all validations statistics. Stem volume tables of Quercus serrata were derived by applying Kozak's model and carbon stock tables in each tree height and DBH were developed with country-specific carbon emission factors ($WD=0.65t/m^3$, BEF=1.55, R=0.43) of Quercus serrata. As a result of carbon stock analysis by age class in Quercus serrata, carbon stocks of IV age class (11,358 ha, 36.5%) and V age class (10,432; 33.5%) which take up the largest area in distribution of age class were 957,000 tC and 1,312,000 tC. Total carbon stocks of Quercus serrata were 3,191,000 tC which is 3% compared with total percentage of broad-leaved forest and carbon sequestration per hectare(ha) was 3.8 tC/ha/yr, $13.9tCO_2/ha/yr$, respectively.

• The Journal of the Petrological Society of Korea
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• v.23 no.3
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• pp.187-195
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• 2014

In this paper, we report new OSL ages of the marine terrace sediments at Suryum fault site, using single grains of quartz, and briefly discuss their chronological implications on the timing of terrace formation along the southeastern coast of Korea. Of 1200 grains measured, 93 quartz grains were found to have OSL properties suitable for dating, the equivalent dose ($D_e$) values of which varied significantly, ranging from 50 Gy to 610 Gy with the overdispersion of $30{\pm}4%$. Applied to the Central Age Model (CAM) and Minimum Age Model (MAM), these quartz grains showed the OSL ages of $83{\pm}4ka$ and $60^{+3}{_{-7}}ka$, respectively, both of which are stratigraphically inconsistent with the previously reported OSL ages of lower $2^{nd}$ terrace (MIS 5a; ~80 ka). However, Finite Mixture Model (FMM) revealed that a small fraction of the measured quartz grains ($6{\pm}4%$) were of the ages ($194{\pm}24ka$) corresponding to MIS 7. Conclusively, based on single grain OSL ages, it would be prudent not to exclude the possibility that the marine terrace sediments at Suryum fault site have formed during MIS 7. Further, our single grain OSL ages imply that multiple grain(single aliquot) OSL dating methods are not applicable to the marine sediments at Suryum fault site.

• Current Optics and Photonics
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• v.1 no.1
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• pp.51-59
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• 2017

In addition to its typical use for skin rejuvenation, fractional laser irradiation of early cancerous lesions may reduce the risk of tumor development as a byproduct of wound healing in the stroma after the controlled injury. While fractional ablative lasers are commonly used for cosmetic/aesthetic purposes (e.g., photorejuvenation, hair removal, and scar reduction), we propose a novel use of such laser treatments as a stromal treatment to delay tumorigenesis and suppress carcinogenesis. In this study, we found that non-ablative fractional laser (NAFL) irradiation may have a possible suppressive effect on early tumor growth in syngeneic mouse tumor models. We included two syngeneic mouse tumor models in irradiation groups and control groups. In the irradiation group, a thulium fiber based NAFL at 1927 nm was used to irradiate the skin area including the tumor injection region with 70 mJ/spot, while no laser irradiation was applied to the control group. Numerical simulation with the same experimental condition showed that thermal damage was confined only to the irradiation spots, sparing the adjacent tissue area. The irradiation groups of both tumor models showed smaller tumor volumes than the control group at an early tumor growth stage. We also detected elevated inflammatory cytokine levels a day after the NAFL irradiation. NAFL treatment of the stromal tissue could potentially be an alternative anticancer therapeutic modality for early tumorigenesis in a minimally invasive manner.