• The Korean Journal of Physiology and Pharmacology
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• v.16 no.3
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• pp.153-158
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• 2012

Cellular effects of ethanol in YD-15 tongue carcinoma cells were assessed by MTT assay, caspase activity assay, Western blotting and flow cytometry. Ethanol inhibited the growth and proliferation of YD-15 cells in a dose- and time-dependent manner in an MTT assay. The effects of ethanol on cell cycle control at low percent range of ethanol concentration (0 to 1.5%), the condition not inducing YD-15 cell death, was investigated after exposing cells to alcohol for a certain period of time. Western blotting on the expression of cell cycle inhibitors showed that p21 and p27 was up-regulated as ethanol concentration increases from 0 to 1.5% whilst the cell cycle regulators, cdk1, cdk2, and cdk4 as well as Cyclin A, Cyclin B1 and Cyclin E1, were gradually down-regulated. Flow cytometric analysis of cell cycle distribution revealed that YD-15 cells exposed to 1.5% ethanol for 24 h was mainly arrested at G2/M phase. However, ethanol induced apoptosis in YD-15 cells exposed to 2.5% or higher percent of ethanol. The cleaved PARP, a marker of caspase-3 mediated apoptosis, and the activation of caspase-3 and -7 were detected by caspase activity assay or Western blotting. Our results suggest that ethanol elicits inhibitory effect on the growth and proliferation of YD-15 tongue carcinoma cells by mediating cell cycle arrest at G2/M at low concentration range and ultimately induces apoptosis under the condition of high concentration.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.37 no.4
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• pp.307-311
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• 2004

Age and growth of red tongue sole (Cynoglossus joyneri), were studied using samples from the coastal waters off Yeosu, Korea, from January to December, 2001. Sagittal otoliths had relatively clear annuli. Marginal index of otolith dropped sharhly in August suggesting that each annulus was formed once a year In August. Monthly changes in the gonadosomatic index indicated that spawning peaked between July and September. The oldest fish observed in this study was 4 years old for both of females and males. Relationships between the otolith radius (R) and total length (L) were: L=14.1R-0.098 for females, and L=11.9R+1.83 for males. Relationships between total length and body weight $(W)\;were:\;W=0.0021L^{3.24}\;for\;females,\;and\;W=0.0014L^{3.39}$ for males. Growth in length of the fish was expressed by the von Bertalanffy's growth equation as:$L_{t}=29.06\;(1-e^{-0.19(t+2.40)})\;for\;females\;and\;L_{t}=27.44 (1-e^{-0.17(t+2.84)})$ for males.

• Phonetics and Speech Sciences
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• v.13 no.2
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• pp.1-7
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• 2021

Speech is a spatiotemporally coordinated structure of constriction actions at discrete articulators such as lips, tongue tip, tongue body, velum, and glottis. Like other human movements (e.g., reaching), each action as a linguistic task is completed by a synergy of involved basic elements (e.g., bone, muscle, neural system). This paper discusses how speech tasks are dynamically related to joints as one of the basic elements in terms of robotics of speech production. Further this introduction of robotics to speech sciences will hopefully deepen our understanding of how speech is produced and provide a solid foundation to developing a physical talking machine.

• Korean Journal of Head & Neck Oncology
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• v.25 no.1
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• pp.28-32
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• 2009

Background : The most frequently reported risk factors for head and neck suamous cell carcinoma are smoking and alcohol. But in a recent overview, human papilloma virus(HPV) infection was revealed the important carcinogenic factor in oropharyngeal cancer. We aimed to clarify whether HPV directly effects on the oncogenesis and biologic behavior of hean and neck squamous cell carcinoma by comparison with infection prevalence, and physical status of virus. Material and Method : We used HPV genotyping DNA chip(Biocore, Korea, Seoul) arrayed by multiple oligonucleotide probes of L1 sequence of 26 types of HPV and HPV genotypes are identified by fluorescence scanner. The copy numbers of HPV E2 and E6 open reading frames(ORF) were assessed using a TaqMan-based 5'-exonuclease quantitative real-time PCR assay. The ratio of E2 to E6 copy numbers was calculated to determine the physical status of HPV-16 viral gene. Results : We observed a significant difference in HPV prevalence between tonsillar cancer group and control group(73.1% vs. 11.6%), and most of the HPVs were type 16(87.2%) and integrated(94.1%) state. In terms of oral tongue cancer, we demonstrate that 30.5% has integrated HPV-16 in cancer tissue. But Glottic cancer only 1% is related to HPV-16 integration. Conclusion : This study revealed significant relationship of HPV prevalence with oropharyngeal and oral tongue squamous cell carcinoma. Most of HPV were 16 type and integrated or mixed, HPV-16 integration could be directly related to the carcinogenesis.

• Applied Microscopy
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• v.37 no.3
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• pp.147-156
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• 2007

The dorsal lingual papillae of Myotis macrodactylus were investigated morphologically using scanning electron microscopy. Three types of lingual papillae from Myotis macrodactylus were recognized. The filiform(Fi) papillae were distributed over the entire dorsal surface of the tongue, and they could be classified into 5 types of papillae by the shape, size and number of the protrusion. Type I was distributed on dorsal surface of the apex in the anterior region of the tongue, has a crow-like shape with ten to fifteen formed projections. Type II was located in the medial portion of the anterior region of the tongue, has an eight to ten spin-like protrusion. Type III was distributed on the medial portion of the tongue, has a scale-like papille with five to seven protrusion. Type IV was distributed on the both lateral portion of the posterior region of the tongue, has a small conical papillae, does not have needle projection. Type V have not needle projection, a large of conical papillae, on the both lateral portion of the posterior region of the tongue. Most Fu(Fungiform) papillae were distributed the both lateral region or medial portion of the posterior region of the tongue, has a round or oval shape with $40{\sim}45$ papillae. The small $(65{\mu}m)$, meddle $(75{\mu}m)$ and large $(120{\mu}m)$ fungiform papillae were distributed on dorsal surface of the apex in the anterior, the both lateral portions and medial portion of the posterior region of the tongue, respectively. Two large oval vallate (V) papillae are located in the medial portion of the posterior region of the tongue. In conclusion, the morphology of lingual papillae is useful to determine species relationship among Myotis species. It suggests that the difference of types and number of lingual papillae caused by the difference of food habit.

• The Korean Journal of Food And Nutrition
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• v.36 no.1
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• pp.58-68
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• 2023

This study investigated the quality characteristics of beyolmijang prepared with corn (steamed and roasted corn) with different pretreatments and simultaneously added starter (Bacillus velezensis JH1). pH decreased, whereas total acidity showed a tendency to increase. The moisture content decreased slightly according to the fermentation period. In color, the L and b values decreased in all samples, whereas the a value showed a tendency to increase significantly. In microorganisms, sample D had more lactic acid bacteria, whereas the mold content was lower. The total polyphenol content was highest at six weeks in sample E, and other samples showed a tendency to decrease over eight weeks. Antioxidant activity increased significantly. In particular, the content in sample E was significantly higher. Reducing sugar showed a tendency to increase as the fermentation period increased. The highest content was found in sample C containing roasted corn at six weeks of fermentation. Amino nitrogen and ammonia nitrogen content increased in all samples. As a result of electronic tongue, sample E prepared with steamed corn, roasted corn, and lactic acid bacteria showed a low salty taste, sour taste, and high umami taste and was considered a good material for the development of byeolmijang.

• Journal of dental hygiene science
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• v.23 no.4
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• pp.378-388
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• 2023

Background: With increasing interest in health in old age, aspects of oral aging are being considered. The Korean Academy of Geriatric Dentistry recently proposed the diagnostic criteria for oral frailty in older adults in Korea. This study aimed to conduct a cross-sectional survey of factors related to oral frailty among community-dwelling older adults and identify differences in oral frailty status according to age and sex. Methods: Among 217 older adults aged ≥60 years who visited a senior center in Wonju, 206 completed all tests for oral frailty. Among them, data from those with a Korean Version of the Modified Barthel Index score ≥90 were used in the final analysis. After evaluating oral frailty diagnostic factors such as chewing ability, occlusal force, tongue pressure, oral dryness, oral cleanliness, and swallowing function, oral hypofunction was determined according to the oral frailty diagnostic criteria. Subsequently, the evaluation results were compared based on sex and age. Results: Significant differences in chewing ability, maximum occlusal pressure, and maximum tongue pressure were observed between sexes. However, these differences did not affect oral frailty diagnosis. All diagnostic factors of oral frailty, except for the risk of oral dryness and swallowing dysfunction, showed significant differences with age. However, no significant difference was observed in the prevalence of oral frailty. Additionally, this study found no relationship between sex and oral frailty factors using the oral frailty diagnostic criteria. However, it also found that age plays a significant role as an oral frailty diagnostic indicator, in addition to oral dryness and swallowing function. Conclusion: Sex and age did not affect oral frailty diagnosis. However, patients' chewing ability, occlusal force, and tongue pressure were affected by sex and age. Therefore, sex and age should be considered when diagnosing and intervening in oral frailty in the future.

• Proceedings of the Acoustical Society of Korea Conference
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• spring
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• pp.69-72
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• 2004

In this paper, we tried to develop a vowel training assistant method using vowel formant statistics. Formant statistics were obtained from PBW set consists of 452 words from 8 persons. Then, we calculated distance from input formants to each center of vowel formant space. Based on the distance, direct ions to correct the speaker's manner of articulation, i .e. position of jaw and tongue.

• Proceedings of the IEEK Conference
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• 2003.11a
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• pp.325-328
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• 2003

In this paper, we tried to develop a vowel training assistant method using vowel formant statistics. Formant statistics were obtained from PBW set consists of 452 words from 8 persons. Then, we calculated distance from input formants to each center of vowel formant space. Based on the distance, directions to correct the speaker's manner of articulation, i.e. position of jaw and tongue.

• Speech Sciences
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• v.11 no.4
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• pp.7-15
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• 2004

In this paper, we develop a vowel training assistant method using vowel formant statistics. Formant statistics were obtained from a PBW set consisting of 452 words from 8 persons. Then we calculated distance from input formants to each center of vowel formant space. Based on the distance, directions could be given to correct the speaker's manner of articulations, i.e. position of jaw and tongue.