• 대한치과의사협회지
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• 제11권1호
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• pp.59-66
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• 1973

• 경영정보학연구
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• 제3권2호
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• pp.337-347
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• 2001

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 1998년도 한국대기보전학회 추계학술대회 발표 논문집
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• pp.201-202
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• 1998

• Annals of Clinical Neurophysiology
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• 제12권1호
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• pp.32-34
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• 2010

• 한국수산과학회지
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• 제34권6호
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• pp.617-623
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• 2001

일본 박다만의 DO농도의 계절변동을 예측하기 위하여 일사량의 확률분포에 의해 생성된 발생가능한 서로 다른 20개의 시간별 일사량의 시계열을 기초로 DO농도변동에 대한 수치예측실험을 실시하여 수층과 저질간의 물질순환 및 일사량의 변동에 따른 DO농도의 계절변동에 대하여 검토하였다. 얻어진 결론을 요약하면 다음과 같다. 수질 및 물질순환의 예측결과 박다만의 동부해역 (HE해역)의 수질은 식물플랑크톤에 의한 내부생산에 크게 영향을 받고 있는 것으로 나타났으며, DIP의 물질수지로부터 저질의 영양염 플럭스가 만내의 수질변화에 영향을 미치고 있는 것으로 나타났다. 일별일사량의 관측자료와 근사식 및 난수발생에 의해 얻어진 CFD곡선은 잘 일치하였다 또한, DO농도가 해수교환정도에 크게 영향을 받고 있는 것으로 나타났으며. 만내부에 위치한 St. E-5에서는 약 1 mg/L의 이상의 농도차를 가진 시계열의 변동을 나타내었다 수치예측실험 20 Case의 DO농도 계산치를 평균하여 얻어진 St. E-2와 St. E-5에서의 최저 DO농도는 각각 4.44mg/L와 4.82mg/L 정도였으며 그 표준편차는 각각 0.1 mg/L와 0.123mg/L로 계산되었다. 어떤 해역에서 발생 가능한 일사량의 조건변화에 따른 DO농도의 시간별 예측은 수산자원의 피해를 최소화할 수 있는 적정 DO 농도의 예측에 합리적으로 이용될 수 있는 수법으로 제시되었다.

• 한국균학회지
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• 제46권4호
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• pp.415-425
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• 2018

다양한 Aureobaisidum속을 발굴하여 흑효모의 유용한 특성을 활용하기 위해 전라도 지역 꽃에서 효모 433균주를 분리하고 분자계통학 및 형태학적 분석을 통해 다양성을 확인하였다. large subunit (LSU) rDNA 염기서열 분석을 기준으로 전라도 지역의 Aureobasidium속을 6그룹으로 분류한 후, LSU와 ITS rDNA 염기서열 분석을 통해 전라도 지역 꽃에서 유래한 효모의 주요 우점종이 Group A와 Group D임을 확인할 수 있었다. GroupB, E, F는 전라남도에만 분포하는 것으로 보아 전라북도에 비해 전라남도에서 다양한 Aureobasidium종이 분포하는 것으로 생각된다. Group A는 A. pullulans, Group B는 A. melanogenum, Group F는 Aureobasidium 신종 후보군으로 분류할 수 있었다. Group C, D, E는 LSU와 ITS rDNA 분석에서 A. leucospermi, A. namibiae, A. subglaciale 사이의 명확하게 분류되지 않았으나 콜로니 형태에서 구분 가능한 특성을 보인 것으로 보아, Aureobasidium은 분자계 통학적 분석방법과 형태적 동정을 병행하여 종 수준 동정을 보완할 수 있을 것으로 생각된다.

• 한국언어정보학회지:언어와정보
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• 제20권1호
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• pp.51-71
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• 2016

The status of the Korean morphological marker '-e ci' has been controversial whether it is a passive marker, an anticausative marker, or a passive/anticausative marker. However, the previous approaches that tried to classify '-e ci' constructions based on the syntactic verb classes (i.e. intransitive or transitive) were short of explaining the properties of the constructions. In this study, the '-e ci' constructions were distinguished based on agentivity, following Levin & Rappaport Hovav (1995) and Alexiadou et al. (2006). Moreover, how the verbal root meaning is associated with the passive/anticausative construction was investigated by means of Distributed Morphology (DM) (Embick 2010; Marantz 1997). I argued that the morphological marker '-e ci' is the instantiation of the absence of external arguments. With respect to the behavior of the Korean '-e ci' constructions with the semantics of each verbal root class, I found out that the '-e ci' constructions can form passives with the verbal roots that require the external arguments; whereas, the anticausatives cannot be formed with the roots that necessarily require the agentive arguments. However, contrary to the previous arguments that '-e ci' passives can be only formed with transitive verbs, it is discovered that non-agentive transitive roots do form anticausatives. Moreover, I argued that there are two types of the anticausatives - zero and '-e ci' anticausatives. Since the valency reduction is marked by the non-active voice morphology, the zero anticausatives appear only with the roots that do not require external arguments. The different '-e ci' constructions (passives, '-e ci', and zero anticausatives) are represented by the distinct syntactic structures. I proposed that the morphological similarity between the passives and the '-e ci' anticausatives is due to the presence of VoiceP, which introduces the external arguments. Moreover, the lack of the voice morphology in the zero anticausatives is explained by the absence of the VoiceP.

• Parasites, Hosts and Diseases
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• 제55권3호
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• pp.337-340
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• 2017

Leishmaniasis is a neglected and endemic disease that affects poorest population mainly in developing countries. A lack of adequate and definitive chemotherapeutic agents to fight against this infection has led to the investigation of numerous compounds. The aim of this study was to investigate in vitro activity of boldine against Leishmania amazonensis murine cell infection. Boldine ((S)-2,9-dihydroxy-1,10-dimethoxy-aporphine) is an aporphine alkaloid found abundantly in the leaves/bark of boldo (Peumus boldus Molina), a widely distributed tree native to Chile. The in vitro system consisted of murine macrophage infection with amastigotes of L. amazonensis treated with different concentrations from 50 to $600{\mu}g/ml$ of boldine for 24 hr. Intracellular parasite destruction was assessed by morphological examination and boldine cytotoxicity to macrophages was tested by the MTT viability assay. When cells were treated with $100{\mu}g/ml$ of boldine the reduction of parasite infection was 81% compared with untreated cultures cells. Interestingly, boldine-treatment caused a concentration-dependent decrease of macrophage infection that culminated with 96% of reduction when cells were submitted to $600{\mu}g/ml$ of boldine. Cell cultures exposed to $100{\mu}g/ml$ of boldine and $300{\mu}g/ml$ of $Glucantime^{(R)}$ during 24 hr showed a significant reduction of 50% in parasitized cells compared with cell cultures exposed just to $Glucantime^{(R)}$. The study showed that treatment with boldine produces a better effect than treatment with the reference antimonial drug, glucantime, in L. amazonensis infected macrophage. Our results suggest that boldine is a potentially useful agent for the treatment of leishmaniasis.

• Journal of Microbiology and Biotechnology
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• 제20권3호
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• pp.643-649
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• 2010

Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. In Korea, an outbreak of aseptic meningitis caused by echovirus type 30 (E30) occurred from May to October in 2008. In order to determine the etiologic agent, CSF and/or stool specimens from 140 children hospitalized for aseptic meningitis at Soonchunhyang University Cheonan Hospital between June and October of 2008 were tested for virus isolation and identification. E30 accounted for 61.7% (37 cases) and echovirus 6 accounted for 21.7% (13 cases) of all the human enteroviruses (HEVs) isolates (60 cases in total). For the molecular characterization of the isolates, the VP1 gene sequence of 18 Korean E30 isolates was compared pairwise using the MegAlign with 34 reference strains from the GenBank database. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the Korean strains differed from those of lineage groups A, B, C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the complete VP1 nucleotide sequences resulted in a monophyletic tree, with eight clustered lineage groups. All Korean isolates were segregated from other lineage groups, thus suggesting that the Korean strains were a distinct lineage of E30, and a probable cause of this outbreak. This manuscript is the first report, to the best of our knowledge, of the molecular characteristics of E30 strains associated with an aseptic meningitis outbreak in Korea, and their respective phylogenetic relationships.

• IMMUNE NETWORK
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• 제13권5호
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• pp.213-217
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• 2013

Enterotoxigenic Bacteroides fragilis (ETBF) is a human gut commensal bacteria that causes inflammatory diarrhea and colitis. ETBF also promotes colorectal tumorigenesis in the Min mouse model. The key virulence factor is a secreted metalloprotease called B. fragilis toxin (BFT). BFT induces E-cadherin cleavage, cell rounding, activation of the ${\beta}$-catenin pathway and secretion of IL-8 in colonic epithelial cells. However, the precise mechanism by which these processes occur and how these processes are interrelated is still unclear. E-cadherin form homophilic interactions which tethers adjacent cells. Loss of E-cadherin results in detachment of adjacent cells. Prior studies have suggested that BFT induces IL-8 expression by inducing E-cadherin cleavage; cells that do not express E-cadherin do not secrete IL-8 in response to BFT. In the current study, we found that HT29/C1cells treated with dilute trypsin solution induced E-cadherin degradation and IL-8 secretion, consistent with the hypothesis that E-cadherin cleavage causes IL-8 secretion. However, physical damage to the cell monolayer did not induce IL-8 secretion. We also show that EDTA-mediated disruption of E-cadherin interactions without E-cadherin degradation was sufficient to induce IL-8 secretion. Finally, we determined that HT29/C1 cells treated with LiCl (${\beta}$-catenin activator) induced IL-8 secretion in a dose-dependent and time-dependent manner. Taken together, our results suggest that BFT induced IL-8 secretion may occur by the following process: E-cadherin cleavage, disruption of cellular interactions, activation of the ${\beta}$-catenin pathway and IL-8 expression. However, we further propose that E-cadherin cleavage per se may not be required for BFT induced IL-8 secretion.