• 한국미생물·생명공학회지
• /
• 제23권3호
• /
• pp.297-303
• /
• 1995

Antitumor antibiotic material was produced by Streptomyces sp. YBE-316 which was isolated from soil, and the optimal culture conditions for the antitumor antibiotic material production were as follows; 2.0% (w/v) sucrose, 0.8% (w/v) polypeptone, 0.4% (w/v) yeast extract, 0.2% (w/v) K$_{2}$HPO$_{4}$, pH 7.0, at 30$\circ$C, 150 rpm and for 100 hours culture. The antitumor antibiotic material had strong antitumor antibiotic activities against most testing tumor cell lines, gram positive and negative bacteria, yeasts, and, especially, Penicillium chrysogenum in fungi.

• Food Science and Biotechnology
• /
• 제14권6호
• /
• pp.875-877
• /
• 2005

Presence of Enterobacter sakazakii, occasional pathogen of powdered infant formula causing rare, but life-threatening diseases such as neonatal meningitis, bacteremia, necrotizing enterocolitis, and necrotizing meningoencephalitis after ingestion was examined in 45 powdered infant formula products manufactured in Korea using chromogenic Druggan-Forsythe-Iversen (DFI) medium, and isolates were identified with API 20E. Ent. sakazakii was isolated from three products. Ent. sakazakii isolates were genotyped by RAPD-PCR using two random primers, and their banding patterns were compared.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1995년도 추계학술발표회논문집(2)
• /
• pp.1021-1026
• /
• 1995

The seismic responses of a base isolated Pressurized Water Reactor (PWR) are investigated using a mathematical model which expresses the superstructure as lumped mass-spring model and the seismic isolator as an equivalent spring-damper. Time history analyses are performed for the 1940 E1 Centre earthquakes in both horizontal and vertical directions. In the analysis, structural damping of 5% is used for the superstructure. The isolator damping ratios of 12% for horizontal and 5% for vertical directions are used. The acceleration responses in base isolated PWR superstructure with high damping rubber bearings are much smaller than those in fixed base structure in horizontal direction. However, the vertical acceleration responses at the superstructure in the base isolation system are amplified to some extent. It is suggested that the vertical seismic responses at the superstructure should be reduced by introducing a soft vertical isolation device.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2002년도 춘계종합학술대회
• /
• pp.234-237
• /
• 2002

Sierpinski 프랙탈 삼각형을 이용한 원형 편파를 가지는 마이크로스트립 패치 안테나를 제안한다. Sierpinski 프랙탈 삼각형은 3개의 정삼각형을 가지는 구조로 이를 이용하여 sequential 회전에 의해 원형 편파를 발생시킬 수 있다. Sierpinski 구조의 $1\times3$ 안테나 배열에 대해 포트간 분리도(isolation) 와 AR(Axial Ratio)의 특성을 살펴보았다.

• Animal cells and systems
• /
• 제15권1호
• /
• pp.19-27
• /
• 2011

Hepatocytes, the major epithelial cells of the liver, maintain their morphology in culture dishes coated with extracellular matrix (ECM) components such as collagen and fibronectin or biodegradable polymers (e.g. chitosan, gelatin). In these coated dishes, survival of cells and maintaining of liver-specific functions may increase. The aim of this study was to determine a suitable, cost-effective and simple system for hepatocyte isolation and culture which may be useful for various applications such as in vitro toxicology studies, hepatocyte transplantation and bioartificial liver (BAL) systems. In order to obtain primary cultures, hepatocytes were isolated from liver by an enzymatic method and cultured on plates coated with collagen, chitosan or gelatin. Collagen, gelatin-sandwich and gelatin-cell mixture methods were also evaluated. Morphology and attachment of the cells were observed by inverted microscope and scanning electron microscope (SEM). An MTT assay was used to determine cell viability and mitochondrial activity.

• 대한미생물학회지
• /
• 제22권2호
• /
• pp.103-108
• /
• 1987

Vibrio vulnificus septicemia is nor-rare diease in Korea. Carriage rate of the orgaism by shellfish is not well known. In this study performance of SPS agar and SGP broth and effect of storage of specimen in the isolaton was determined using the shellfish specimens collected from the coast and market of Koonsan city. Isolation rate was similar with TCBS and with SPS, but the rate became much highher after enrichment in SGP broth. 80% of oyster speimes were positve when inoculated immediately, but the rate dropped rapidly after storage of specimens at freezing temperature for sometime. All of the isolates fermented lactose in 2 days. A few isolates were not identfiable with API 20E system, because of acid prduction from melibiose. Serover 04 was the frequent isolates.

• ETRI Journal
• /
• 제45권4호
• /
• pp.581-593
• /
• 2023

This paper proposes four-ring slot resonator-based MIMO antennas of 75×150 mm² without and with CSRR structures in the sub-6-GHz range. These orthogonal-fed antennas have shown diverse characteristics with dual polarization. L-shaped parasitic structures have increased the isolation (i.e., >40 dB) in the single-element antenna over the band of 3.4 GHz-3.8 GHz. A set of three CSRR structures in the MIMO antenna reduced the coupling between antenna ports placed in an inline arrangement and enhanced the isolation from 12 dB to 20 dB and the diversity characteristics. The S-parameters of both MIMO antennas are measured and used to evaluate MIMO parameters like ECC, TARC, MEG, and channel capacity loss. The simulation results show the variations in the gain and directivity on exciting linear and dual polarizations. The diversity performance of the reported MIMO antennas is suitable for 5G applications.

• 산업식품공학
• /
• 제23권3호
• /
• pp.193-199
• /
• 2019

식품첨가물등급의 protease를 이용하여 쌀가루로부터 쌀전분을 분리하는 효소적 쌀전분 분리·정제법을 구축하기 위해 protease의 반응시간, 반응온도와 농도를 요인으로 하여 변형된 23 완전요인설계법에 따라 protease 반응조건들을 설계하고 이에 따른 쌀전분들의 수율을 조사하였다. 설계된 반응조건들에 따라 제조된 쌀전분들의 수율들에 기초한 반응표면분석을 통해 쌀전분 수율에 대한 protease 반응조건들의 영향을 조사하였다. 또한 효소적 분리·정제법에 의한 쌀전분들의 상업적 활용도를 평가하기 위해 알칼리 침지법에 의해 제조된 쌀전분(대조군)과 물리화학적 특성을 대해 비교 분석하였다. Protease를 이용한 효소적 분리·정제법에 의한 쌀전분들의 수율은 대조군보다 낮았지만 그 상대적 순도는 높은 수준을 나타내었다. Protease에 미량함유되어 있는 amylase 계통의 효소들에 의한 쌀전분의 부분적인 손상이 예상됨에도 1.5% protease를 이용하여 15℃에서 24시간 동안 처리하여 제조된 쌀전분(RST2)의 아밀로오스 함량은 대조군의 것과 유의적인 차이를 보이지 않았다. 용해도는 효소적 분리·정제법에 의한 쌀전분들이 대조군보다 유의적으로 높은 수준을 나타내었다. 팽윤력은 RST2와 0.5% protease를 이용하여 15℃에서 24시간 동안 처리하여 제조된 쌀전분(RST3)이 대조군과 유의적인 차이를 보이지 않았다. 호화온도는 대조군에 비해 효소적 분리·정제법에 의한 쌀전분들이 높은 수준을 나타내었으나 호화엔탈피는 유의적으로 낮은 수준이었다. 페이스팅 점도는 대조군에 비해 효소적 분리·정제법에 의한 쌀전분들이 모든 온도프로파일에 있어 낮은 수준을 나타내었다. 이와 같은 결과들은 효소적 분리·정제법에 사용된 protease에 미량 함유되어 있는 amylase 계통의 효소들에 의한 쌀전분의 부분적인 손상과 protease 처리하는 동안 쌀전분에 있어 annealing이 진행된 결과인 것으로 생각된다. 그럼에도 본 연구에서 효소적 분리·정제법에 의해 제조된 쌀전분들은 높은 고형분 함량을 요구하며, 페이스트의 겔화 또는 노화의 진행이 지연되는 특성을 가공식품의 원료로 적합한 것으로 판단된다. 따라서 효소적 분리·정제법에 의한 쌀전분들은 기존의 알칼리 침지법에 의한 쌀전분과는 다른 특성을 보유한 쌀전분 소재로서 활용가능성이 있을 것으로 생각된다. 또한 효소적 분리·정제법은 알칼리 침지법에 비해 쌀전분의 제조 시간을 단축할 수 있으며, 고농도의 염용액을 배출하지 않아 경제적인 방법인 것으로 판단된다.

• 대한미생물학회지
• /
• 제14권1호
• /
• pp.17-25
• /
• 1979

Bacteriologic diagnosis of enteric infection remains to be an important role of clinical laboratory because of the prevalence of the infection. Often the determination of etiologic agent and its susceptibility to antibiotics are of vital importance for a proper management of the infection. In our previous paper, an analysis of the isolation of enteric pathogens for the years 1969-73 was reported to clarify the status of those years. The present analysis was made based on the data obtained during the years 1974-78, to see if any change of the status was rendered. 1. During the 5-year period, from the cultures of 7,308 stool or rectal specimens 833 patients yielded enteric pathogens: 468 Shigella, 295 Salmonella, 30 Vibrio parahaemolyticus and 40 enteropathogenic Escherichia coli(EPEC). 2. Of the 295 Salmonella, 271 were S. typhi Isolation of 12 S.paratyphi-A, 1 Salmonella group B, 4 group C, 5 group D and 2 group E meant a definite increase of these sero-groups, S. typhi was most frequently isolated in August and in December, and from 30- to 39-year-old patients. 3. Of the 468 Shigella, 10 were subgroup A, 338 subgroup B, 3 subgroup C and 117 subgroup D. Most of the subgroup B belonged to type 1,2, or 3. The proportion of S. sonnei decreased from 31.3% in 1974 to 18.2% in 1978. In foreign patients, S. sonnei remained to be the frequntly isolated species. Shigella isolation was frequent in August and in 2- to 5-year-old patients. 4. V. parahaemolyticus was isolated from 30 and EPEC from 40 patients. 5. Ninty-nine per cent and 99.5% of the S. typhi isolates were susceptible to chloramphenicol and to ampicillin respectively. 92.8% of S sonnei were susceptible to ampicillin. S. flexneri type 2 was notable for their markedely decreased proportion being susceptible to ampicillin: 84.4% in 1974 and 25.6% in 1978.

• 한국통신학회논문지
• /
• 제30권4A호
• /
• pp.350-357
• /
• 2005

근거리무선통신 (Dedicated Short Range Communication, DSRC)은 지능형교통시스템 서비스 제공을 위한 통신 수단으로, 수 미터에서 수백 미터인 근거리 영역의 노변장치(Road Side Equipment, RSE)와 차량탑재장치(On-Board Equipment, OBE)와의 양방향 고속통신을 수행하는 통신시스템이다. 본 논문에서는 SiGe HBT 공정을 이용하여 근거리무선통신 송신기용 5.8 GHz 상향믹서를 설계 및 제작하였다. 설계된 상향믹서는 믹서코어 회로와 더불어 IF/LO/RF 입출력 정합 회로, IF/LO 입력 발룬 회로와 RF 출력 발룬 회로가 단일칩으로 구현되었다. 제작된 상향믹서는 $2.7 mm\times1.6mm$의 크기를 가지며, 3.5 dB의 전력변환이득과 -12.5 dBm의 OIP3, 42 dB의 LO to E isolation, 38 dB의 LO to RF isolation, 3.0 V의 공급전압 하에서 29 mA의 전류소모로 측정되었다.