• The Korean Journal of Physiology and Pharmacology
• /
• 제22권6호
• /
• pp.705-712
• /
• 2018

The tube formation assay is a widely used in vitro experiment model to evaluate angiogenic properties by measuring the formation of tubular structures from vascular endothelial cells (ECs). In vitro experimental results are crucial when considered the advisability of moving forward to in vivo studies. Thus, the additional attentions to the in vitro assay is necessary to improve the quality of the pre-clinical data, leading to better decision-making for successful drug discovery. In this study, we improved the tube formation assay system in three aspects. First, we used human endothelial colony forming cells (ECFCs), which are endothelial precursors that have a robust proliferative capacity and more defined angiogenic characteristics compared to mature ECs. Second, we utilized a real-time cell recorder to track the progression of tube formation for 48 hours. Third, to minimize analysis error due to the limited observation area, we used image-stitching software to increase the microscope field of view to a $2{\times}2$ stitched area from the $4{\times}$ object lens. Our advanced tube formation assay system successfully demonstrated the time-dependent dynamic progression of tube formation in the presence and absence of VEGF and FGF-2. Vatalanib, VEGF inhibitor, was tested by our assay system. Of note, $IC_{50}$ values of vatalanib was different at each observation time point. Collectively, these results indicate that our advanced tube formation assay system replicates the dynamic progression of tube formation in response to angiogenic modulators. Therefore, this new system provides a sensitive and versatile assay model for evaluating pro- or anti-angiogenic drugs.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2006년도 추계학술대회논문집
• /
• pp.792-796
• /
• 2006

In recent years, the semiconductor industry and the optical industry is developed rapidly. The recent demand has expanded for optical components such as a optical lens, a optical semiconductor and a measuring instrument. Object transport systems are driven typically by the magnetic field and the conveyer belt. Recent industry requires more faster and efficient transport system. However, conventional transport systems are not adequate for transportation of optical elements and semiconductors. Because conveyor belts can damage precision optical elements by the contact force and magnetic systems can destroy the inner structure of semiconductor by the magnetic field. In this paper, the levitation transport system using ultrasonic wave is developed for transporting precision elements without damages. This transport system is using 2-mode ultrasonic wave excitation and flexural beam modes shapes are evaluated. It compared simulation results with experimental results

• 미생물학회지
• /
• 제4권1호
• /
• pp.1-13
• /
• 1966

The author examined for observing the structures of pyrenoid and cell wall of three strains of Chlorella ellipsoidea and relation of pyrenoid to starch grain formation at the ultrastructure level. 1. The development of pyrenoid of Chlorella species from the time of its initiation and its subdetail sequent activities are described in some pictures. 2. Close correlation between the findings of light microscopy and electron microscopy is proved. 3. The pyrenoid is a dynamic organellae which continues to change its appearance thoughout the development of the Chlorella cell. 4. The starch grains are continously formed by deposition of carbohydrate within the chloroplast with the aid of pyrenoid factors. 5. Some parental starch grains are passed on the daughter cell during cell division. 6. The Da stage cells contain only chlaroplast without pyrenoid matrix. In Da stage a pyrenoid is surrounded by starch and starch grains appear in chloroplast lamellae. In $L_1L_2$ stages, large starch grains of lens form accumulate in cell. In $L_3$ stage pyrenoid disappears for a time and starch grains are scattered. In cell division stage starch grains are divided into four groups. In $L_4$ stage, pyrenoid substance appears temporarily and disappears soon. At this stage the cell is constituted of Dn cell containing chloroplast only. 7. The cellular boundary of JE strain except Y 815 and Y 511 strain contains 250.angs. intermediate layer of unknown chemical composition between the fibrillar cellulose wall and the out capsule layer.

• 한국소음진동공학회:학술대회논문집
• /
• 한국소음진동공학회 2002년도 추계학술대회논문집
• /
• pp.1010-1014
• /
• 2002

A new type actuator has been designed and investigated to overcome thermal problems in slim optical disc drive which is adopted in mobile storage devices. Recently, in optical storage device technical trends, the size of optical disc drives is slimmer to adopt notebook computer and the spindle rotate velocity is faster to achieve high transfer rate and the power of actuator is higher to perform tilting, etc. However, these trends of optical disc drives tend to raise the environment temperature of drives, actuator power and parts temperature. Moreover, it is more difficult to remove the heat inside a drive and the temperature of an actuator increases and drive slims. As a result, increase of surface temperature of actuator body caused that second resonance of an actuator moves down to a lower frequency band and the performance of optical parts also deteriorates. Especially objective lens, coil and magnet of the actuator parts are easily damaged. To manage these thermal problems, in this paper an actuator with a hybrid blade, which is composed of vectra which has low thermal conductivity and magnesium which has high thermal conductivity, has been suggested and verified. Despite the high temperature environment, the proposed actuator showed good dynamic performance.

• 한국정밀공학회지
• /
• 제23권4호
• /
• pp.183-190
• /
• 2006

Digital 3D Real Object Duplication System (RODS) consists of 3D Scanner and Solid Freeform Fabrication System (SFFS). It is a device to make three-dimensional objects directly from the drawing or the scanning data. In this research, we developed an office type SFFS based on Three Dimensional Printing Process and an industrial SFFS using Dual Laser. An office type SFFS applied sliding mode control with sliding perturbation observer (SMCSPO) algorithm for control of this system. And we measured process variables about droplet diameter measurement and powder bed formation etc. through experiments. In case of industrial type SFFS, in order to develop more elaborate and speedy system for large objects than existing SLS process, this study applies a new Selective Dual-Laser Sintering (SDLS) process and 3-axis Dynamic Focusing Scanner for scanning large area instead of the existing f lens. In this process, the temperature has a great influence on sintering of the polymer. Also the laser parameters are considered like that laser beam power, scan speed, and scan spacing. Now, this study is in progress to evaluate the effect of experimental parameters on the sintering process.

• 한국소음진동공학회논문집
• /
• 제21권2호
• /
• pp.178-185
• /
• 2011

Optical image stabilization is a device to compensate the camera movement in the exposure time. The compensation is implemented by movable lens or image sensor that adjusts the optical path to the camera movement. Generally, the camera is moved by a handshake, thus the handshake is considered as an external disturbance. However, there are many other vibrations such as car and train vibration. In this paper, the optical image stabilization system in high frequency region is presented. Notch filter and lead compensator are designed and applied to improve the stability without changing the actuator. To verify the performance of the optical image stabilization system in high frequency region, the experiment equipment with moving object is established. It is confirmed that the opticalimage stabilization system does not diverge at the resonance frequency.

• 한국생산제조학회지
• /
• 제21권3호
• /
• pp.479-486
• /
• 2012

This paper presents a high-speed automatic micro-alignment system that is a part of an inspection machine for small-sized molded lenses of mobile phones, palm-top computers, and so on. This work was motivated by the shortcomings of existing highest-grade commercial machine. A simple tip/tilt/Z parallel mechanism is designed based on kinematic couplings, which is a 3-degree-of-freedom (3-DOF) moderate-cost alignment stage. It is used to automatically adjust the posture of each lens on the tray, which is impossible by the conventional instrument. Amplified piezoelectric actuators are used to ensure the accuracy and dynamic response. Forward kinematic analysis and simulation show that the parasitic motion is small enough compared to the actuator stroke. From the workspace analysis of the moving platform, it is clear that the output motion range satisfies the design requirements.

• 정보저장시스템학회:학술대회논문집
• /
• 정보저장시스템학회 2005년도 추계학술대회 논문집
• /
• pp.190-194
• /
• 2005

There is a great demand of micro-actuators for mobile information devices such as SFF optical drives and mobile camera phones. However, conventional magnetic coils of electromagnetic motors are a major obstacle for miniaturization because of their complicated structures and large power consumption. In this paper, a linear ultrasonic motor to actuate focusing lens of mobile devices is proposed. The new actuator uses a ring type bimorph piezoelectric material, and $d_{31}$ mode is adopted for applying linear motion. The interaction between inertia force and friction force makes linear motion by high-frequency saw signal input. The saw signal gives steady forces on the one direction by asymmetric inclination property of the signal itself on time domain. A commercial FEM (ANSYS) was used in this investigation for simulating structural analysis, identification of dynamic property, such as resultant displacement and coupled analysis with piezoelectric material. To evaluate the performance of the new design, a prototype was manufactured and experiments were carried out. Experimental results show the actuator motion of 1.52 mm/s at 10 kHz input signal in 5 V.

• 정보저장시스템학회논문집
• /
• 제7권2호
• /
• pp.70-74
• /
• 2011

The mobile camera module is a device to be inserted in the digital device for camera feature. The mobile camera module is being shaken by vibrations such as handshake during the exposure time. The clarity is compromised by these vibrations, thus the vibration is considered as an external disturbance. Moreover the use of mobile camera module has been being expanded for automotive vibration should be considered. These external disturbances can cause image blurring, thus optical image stabilization should be applied for image compensation. The compensator is fulfilled mechanically by movable lens group or image sensor that adjusts the optical path to the camera movement. Open loop control is useful for well-defined systems like compliant mechanism. Notch filter and lead compensator are designed and applied to improve the stability and bandwidth. The final level of image compensating is confirmed by image processing with MATLAB and CODE V to verify the better performance.

• 한국소음진동공학회논문집
• /
• 제18권1호
• /
• pp.11-19
• /
• 2008

This paper proposes small VCM(voice coil motor) type, auto-focusing and zoom actuators for mobile information devices. In order to meet the large output displacement within small height restriction, the proposed auto-focusing actuator adopts curved suspensions, which are similar to a leaf-spring type suspension of optical disk drives. The sensitivity of design parameters on output displacement and dynamic performance is implemented using ANSYS (3D FEM tool) to determine the optimal geometry and stiffness of the curved suspensions. This paper also investigates a new zoom actuator without a suspension supporting a bobbin. The zoom actuator uses a moving rail and a stoper mechanism by generating rotational force at lens holder. Magnetic flux density of the zoom actuator are calculated by both the FEM and permeance method. Experiments using prototypes of the proposed focusing and zoom models show that both actuators meet the required displacement and performance.