• Title/Summary/Keyword: Dye visualization

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EXPERIMENTAL STUDY ON TURBULENT SWIRLING FLOW IN A CYLINDRICAL ANNULI BY USING THE PIV TECHNIQUE

  • Chang, T.H.
    • International Journal of Automotive Technology
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    • v.5 no.1
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    • pp.17-22
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    • 2004
  • An experimental investigation was conducted to study the characteristics of turbulent swirling flow in an axisymmetric annuli. The swirl angle measurements were performed using a flow visualization technique using smoke and dye liquid for Re=60,000-80,000. Using the two-dimensional Particle Image Velocimetry method, this study found the time-mean velocity distribution and turbulence intensity in water with swirl for Re=20,000, 30,000, and 40,000 along longitudinal sections. There were neutral points for equal axial velocity at y/(R-r)=0.7-0.75, and the highest axial velocity was recorded near y/(R-r)=0.9. Negative axial velocity was observed near the convex tube along X/(D-d)=3.0-18.0 for Re=20,000.

Study on the flow characteristics and FFT analysis to the pressure fluctuation in a T-type ball valve (T형 볼밸브 내의 유동특성 해석 및 압력변동의 FFT 분석)

  • Yeo, Chang-Ho;Heo, Hyeung-Seok;Suh, Yong-Kweon
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2003.05a
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    • pp.240-244
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    • 2003
  • In this paper, we present the CFD and experimental results for flow characteristics as well as pressure fluctuation in a T-type ball valve. In the experiment, flow visualization for the wake is performed with a fluorescent dye. In CFD, the valve flow is simulated three-dimensionally using a commercial fluid analysis code, FLUENT 6.0. FFT analysis to the pressure fluctuation in the wake region is also calculated Analysis results show that the characteristic frequency of the wake flow is strongly dependent on the Reynolds number.

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An Experimental Study on Swirling Flow with Heat Transfer in the Horizontal Circular Annuli

  • Chang Tae-Hyun
    • Journal of Advanced Marine Engineering and Technology
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    • v.29 no.3
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    • pp.260-274
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    • 2005
  • An experimental investigation was performed to study the characteristics of turbulent swirling flow in the cylindrical annuli. The swirl angle measurements were performed by flow visualization technique using smoke and dye liquid. By using the particle image velocimetry method. this study has found the time-mean velocity distribution and turbulent intensity with swirl for Re=20,000. 30.000. 50.000. and 70,000 along longitudinal sections. The results appear to be physically reasonable. Other experimental study was performed to investigate heat transfer characteristics of turbulent swirling air flow in axisymmetric annuli. The static pressure. the local air flow temperature, and the wall temperature with decaying swirl were measured by using thermocouples and the friction factor and the local Nusselt number were calculated for Re=30,000. 50,000 and 70000. The local Nusselt number was compared with that obtained from the Dittus-Boelter equation with swirl and without swirl, respectively. The results showed that the swirl enhances the heat transfer at the inlet and the outlet of the test tube.

Site-Specific Labeling of Proteins Using Unnatural Amino Acids

  • Lee, Kyung Jin;Kang, Deokhee;Park, Hee-Sung
    • Molecules and Cells
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    • v.42 no.5
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    • pp.386-396
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    • 2019
  • Labeling of a protein with a specific dye or tag at defined positions is a critical step in tracing the subtle behavior of the protein and assessing its cellular function. Over the last decade, many strategies have been developed to achieve selective labeling of proteins in living cells. In particular, the site-specific unnatural amino acid (UAA) incorporation technique has gained increasing attention since it enables attachment of various organic probes to a specific position of a protein in a more precise way. In this review, we describe how the UAA incorporation technique has expanded our ability to achieve site-specific labeling and visualization of target proteins for functional analyses in live cells.

Flow Changes by Stent Insertion in Fusiform Aneurysm Models (스텐트 삽입에 의한 방추형 동맥류 내부 유동의 변화)

  • 이계한;서남현
    • Journal of Biomedical Engineering Research
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    • v.22 no.6
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    • pp.535-542
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    • 2001
  • Endovascular embolization technique using a steno is currently used to treat the wide neck aneurysm. Since intraaneurysmal flow characteristics affect thrombus formation and embolisation process. flow visualization technique incorporating photochromic dye was used to elucidate hemodynamic changes by stenting Inside the fusiform aneurysm models. Qualitative observation of flow field and measurement of wall shear rates were Performed at five aneurysm wall locations under pulsatile flow. Intraaneurysmal flow motion was reduced and sluggish vortical motion was maintained during late deceleration phase by stenting. Also wall shear rates were reduced and OSI's were increased in the stented model. These flow characteristics Provide hemodynamic environment favorable for thrombus formation and intimal hyperplasia. The results of this study show hemodynamic changes by stenting Promote thrombus formation and aneurysm embolisation.

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Process Improvement of Land System Engine for Visualization and Increasing Identification of Engine Oil Leakage (엔진오일 누유 가시화 및 식별력 증대를 위한 기동화력장비 엔진 공정개선)

  • Seo, Suk Ho;Kim, Ji Hoon;Oh, Dae San
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.3
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    • pp.321-327
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    • 2020
  • Engine oil leaks comprise the largest percentage of the major quality problems that have occurred during the engine production process for military land system equipment over the last two years. To minimize this, it is essential to understand the root cause of the engine oil leakage and perceive the more common leakage area, as well as reduce the quality costs of pre-identification for leaks in the engine assembly process and single items. Therefore, in this study, the mass production quality of engines installed and operated in power equipment was improved by visualizing the engine oil leakage injected with the fluorescent dye and increasing the identification of the engine oil leak phenomenon. For this reason, the possibility of substituting the engine oil was considered and the engine oil was injected with the fluorescent dye. The maintenance was improved by reforming the engine oil injection process and leakage inspection process. Finally, these results suggest that the quality costs between mass production processes can be minimized by ensuring the reliability of military engines by reducing the user's complaints and improving the quality control capabilities of engine manufacturers.

Influence of Design of Turbulence Generator on Flow Behavior in Pilot Headbox

  • Youn Hye Jung;Lee Hak Lae;Chin Seong Min
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.36 no.5 s.108
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    • pp.1-10
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    • 2004
  • The geometry of headboxes is of great importance in obtaining good formation, even basis weight profile and fiber orientation. Therefore, many attentions have been made to examine the influence of the geometry of headboxes on the flow behavior. In this study, to evaluate flow behavior in headboxes, three types of turbulence generators were examined using pilot headbox. Velocity profiles in MD and CD were measured using a pressure monitoring system and flow in headboxes was visualized by dye injection method. CD velocity profiles at three different locations inside the slice of Type A headbox showed that the velocity increased downstream to slice exit and had a pattern with four humps due to the persisting wall effect of step diffusors. Results from the evaluation of normalized velocity profile and flow visualization showed that L-shaped Type C headbox caused a large pressure drop but it lacked in flow stabilizing ability.

Solid Graphic Expression in Fashion Illustration Using CAD (CAD를 이용한 패션 일러스트레이션의 회화적 입체표현에 관한 연구)

  • 신상무;박영옥
    • Journal of the Korean Society of Costume
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    • v.44
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    • pp.131-141
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    • 1999
  • The purpose of this study was to investigate the various expressions with materials in fashion illustration using CAD comparing with hand work. The design motive for this study is to be selected from Bell Epoque era which was the revolutionary period in fashion illustration. The results of this study were as follows: 1. By using CAD, water color was well expressed to repeat brushing for the clarity, darkness, and brightness. It was more effective to use CAD in layering or duplicating complex and intricate patterns because the base color gets to be concealed under the repeated oil pastel. Acryl, like oil pastel, was easily absorbed in canvas, so it is effective to repeat brushing for expressing pure color. It was inconvenient to use wax crayon for controlling the moderate opacity because wax crayon absorbed water color dye stuffs, so crayon line was concealed when repetitions were being done. 2. The advantage of using CAD was convenience for getting rid of troublesome process and inefficient works. Also, CAD had a good tool like oil pastel in the use of coloring work by using pure color. By using CAD, various expressions on materials and texture of surface can be achieved effectively. Also, it is very strong substitute for time-saving, convenience, economic aspects from providing simple instrument, and production in the state of various kinds of paper and canvas as a method of visualization. Therefore, fashion illustration using CAD provides effective way of producing works, and gives promising vision in the future.

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Application of Micro-Thin Laser sheet and Mixed Solvent for Micro-LIF Measurement in a Microchannel (마이크로 채널 내부의 Micro-LIF 측정을 위한 마이크로 레이저 평면빔과 혼합용매의 적용)

  • Yoon Sang Youl;Kim Jae Min;Kim Kyung Chun
    • 한국가시화정보학회:학술대회논문집
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    • 2004.11a
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    • pp.86-89
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    • 2004
  • One most feasible way to measure the concentration field in the micro-channel is using micro-LIF(Laser Induced Fluorescence) method. However, an accurate concentration field at a given cross plane in a micro-channel has not been successfully achieved so far due to various limitations in the light illumination and fluorescence signal detection. The present study demonstrates a novel method to provide an ultra thin laser sheet beam having five(5) microns thickness by use of a micro focus laser line generator. The laser sheet beam illuminates an exact plane of concentration measurement field to increase the signal to noise ratio and considerably reduce the depth uncertainty. Nile Blue A was used as fluorescent dye for the present LIF measurement. The enhancement of the fluorescent intensity signals was performed by a solvent mixture of water $(95\%)$ and ethanol (EtOH)/methanol (MeOH) $(5\%)$ mixture. To reduce the rms errors resulted from the CCD electronic noise and other sources, an expansion of grid size was attempted from $1\times1$ to 3(3 or 5(5 pixel data windows and the pertinent signal-to-noise level has been noticeably increased accordingly.

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Bacterial Pattern Formation in Response to the Stiffness of Substrates (접촉 표면의 강성 변화에 따른 박테리아의 군집 패턴)

  • Kim, J.K.;Park, E.J.;Kim, S.R.;Cho, M.Y.;Han, H.
    • 한국가시화정보학회:학술대회논문집
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    • 2007.11a
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    • pp.88-91
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    • 2007
  • Animal cells show different behaviors in response to the mechanical properties of the substrates. We hypothesize that the rigidity of the substrates also affects the bacterial motility and controls the colony dynamics. It is found that the colony size of Escherichia colis and Bacillus subtilis grown on the agar plates is correlated with agarose gel concentrations and thus with the substrate rigidity. High- resolution microscopic imaging reveals that bacteria in single colonies form different aggregation patterns on the agar plates with varying gel concentration. We measured the apparent diffusion coefficients in the agarose gel plates made with different gel concentrations. Mathematical modeling and quantitative imaging of dye dispersion in the agar plates suggest that there is a close connection between the diffusion rate and the colony size. Nanoscale pore structures and kinetic constraints in the porous media may have an effect on bacterial colony dynamics.

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