• Restorative Dentistry and Endodontics
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• v.15 no.2
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• pp.122-126
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• 1990

To examine the microleakage of posterior esthetic restorative materials, using 2% methylene blue dye solution, dye penetration was determined with 80 permanent posterior teeth after thermocycling. The following results were obtained. 1. Amalgam group showed the most severe dye penetration of all test groups through the enamel and dentin / cementum margins. 2. Clearfil group showed much more dye penetration than P-50 at the enamel margin and than resin inlay system at the dentin / cementum margin. 3. Through the etched enamel margin, P-50 and resin inlay system groups showed no dye penetration, if any, but through the dentin / cementum margin, all test groups showed dye penetration. 4. Resin inlay system showed the least dye penetration of all test groups through the dentin / cementum margin.

• Restorative Dentistry and Endodontics
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• v.29 no.5
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• pp.413-422
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• 2004

Established microleakage tests have their own disadvantages. In this study, 3D reconstruction method was tried to overcome these disadvantages. Four types of microleakage tests were used and relationships among them were estimated: penetrated dye volume: marginal adaptability: degree of dye penetration and relative penetrated length to cavity wall. Twenty-four Class V cavities were bulk filled with composite (Esthet X) following surface treatments: N group (no treatment): E group (etching only): T group (etching + Prime & Bond NT). 50% silver nitrate was used as a dye solution after thermocycling ($5^{\circ}C{\;}&{\;}55^{\circ}C$, 1.000 times). Teeth were serially ground with a thickness of 0.2 mm. Volume of dye penetration was estimated from a three-dimensionally reconstructed image with a software (3D-DOCTOR). Percentage of margin without gap was estimated from SEM and degree of dye penetration and the relative length of dye penetration to overall cavity wall were also estimated. ANOVA and Scheffe test for dye volume, Kruskal-Wallis and Mann-Whitney test for marginal quality, Spearman's rho test for checking of relationships among methods were used. The results were as follows: 1. Dye penetration could be seen from several directions, furthermore, its volumetric estimation was possible. 2. Reverse relationship was found between dye volume and marginal quality (r = -0.881/ p = 0.004). 3. Very low relationship was seen between dye volume and two-dimensional tests (degree of dye penetration and relative length). However, 2D evaluation methods showed high relationship (p = 0.002-0.054) each other. 4. Three times vertical section could be recommended as a 2D test.

• Restorative Dentistry and Endodontics
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• v.26 no.5
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• pp.379-386
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• 2001

This study was performed to evaluate the sealing ability of various retrograde filling materials by using bacterial penetration and dye penetration test. One hundred and forty extracted human teeth with single, straight canals and mature apiece were collected and used for this study. All canals were instrumented using an engine driven Ni-Ti file (ProFile). After removing 3mm from the apex of tooth, a standardized 3mm root end cavity was prepared using an ultrasonic instrument. The 70 teeth were randomly divided into 7 groups : 6 groups for retrograde filling using Super-EBA, ZOE, Chelon-Silver, IRM, ZPC and amalgam. The 7th group was used as a negative control. Nail varnish was applied to all external root surfaces to the level of the reseated root ends to prevent lateral microleakages. The specimens were then sterilized in an ethylene oxide sterilizer for 24 hours. 2 mm of the reseated root was immersed in a culture chamber containing a Tripticase Soy Broth with a phenol red indicator. The coronal access of each specimen was inoculated every 72 hours with suspension of Proteus vulgaris. The culture media were observed every 24hours for color change indicating bacterial contamination. The specimens were observed for 4weeks. The remaining 70 teeth were submitted to a dye penetration test. The canals of all teeth were first sealed with AH26 and obturated using an Obtura II system. Root resection, root end preparation and retrograde filling was performed as above. All specimens were suspended in 2% methylene blue dye for 72 hours before being ion gitudinally split. The degree of dye penetration was then measured using a stereomicroscope at 10 magnification and evaluated. The results were as floows : 1. In the bacterial penetration, the degree of leakage was the lowest in the Super-EBA, followed by, in ascending order, ZOE, Chelon-Silver IRM and ZPC. The amalgam showed highest bacterial leakage of all(p<0.01). 2. In the dye penetration, the degree of microleakage was the lowest in the Chelon-Silver and Super-EBA, followed by, in ascending order, IRM, ZPC. The ZOE and amalgam showed the highest microleakage of all (p<0.05). These results suggested that the eugenol based cement, Super-EBA, have excellent sealing ability as a retrograde filling material.

• Restorative Dentistry and Endodontics
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• v.31 no.5
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• pp.337-343
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• 2006

The purpose of this study was to estimate the relation between techniques used for microleakage from dye penetration and for marginal adaptation from SEM evaluation of the restoration. Using high speed #330 bur class V cavities ($4{\times}3{\times}1.5 mm$ around CEJ) were prepared on the buccal surface of 20 extracted human molars. Six dimples as reference points for SEM and dye penetration evaluation were made with 1/2 round bur. Cavity was bulk filled with microhybrid composite resin (Esthet X) and all-in-one adhesive (Xeno III). Teeth were stored in saline solution for one day, after then, they were finished and polished using Sof-Lex system. Fifty percent silver nitrate dye solution was used for the evaluation of microleakage and resin replica was used for marginal adaptation. All of these were done after 1000 times thermocycling between 5 and $55^{\circ}C$. Vertical sections were made through three dimples of restoration to obtain samples for the evaluation of dye penetration and inner marginal adaptation. Outer adaptational estimation was done with an intact restoration before sectioning. Dye penetration was determined in three degrees and percentage of outer and inner leaky margin was estimated from SEM image The data were analysed statistically: Spearman's rho test were used to check relationships between two methods. The result were as follows : 1. There were significant relationships between degree of dye penetration and inner and outer marginal adaptations each (p < 0.01). 2. However, there was no significant relationship between the results or inner and outer marginal adaptation. Within the results of this study, relationship between the percentage of marginal adaptation and microleakage shows significant relationship. However, inner and outer marginal adaptation did not show any significant relationship mutually.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.11a
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• pp.719-724
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• 2001

In this study, wedge splitting tensile test(WST) using dye penetration method was carried out to investigate cracking criterion and fracture characteristics of concrete. For the this purpose, three levels of compressive strength of 180, 300 and 600 kgf/$\textrm{cm}^2$ and five testing age of 1, 3, 7, 14 and 28 days were selected as test variables. The specimen was loaded in a controlled manner and then dye was inserted at the load of 40%, 70% of the presumed peak load and at the load of 90% just after peak load. The fracture process zone was measured at each load step of a specimen. Test results were compared with analytic results by linear elastic fracture mechanics(LEFM) and numerical results through fictitious crack model(FCM) and finite element method(FEM).

• Restorative Dentistry and Endodontics
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• v.19 no.2
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• pp.585-601
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• 1994

The purpose of this study was to evaluate the ability of several intracoronal base materials to prevent cervical leakage of a bleaching agent into the dentinal tubules and along the root canal. In this study, thirty-two anterior teeth were used. After lingual access was prepared in each tooth, tooth was instrumented with a step-back technique to a Nos. 40-50 using K-type files. All teeth were obturated with a lateral condensation technique. Excess gutta percha was removed with a warm instrument to the facial level of the CEJ. Teeth were divided into four groups : Teeth in control group were not filled with base material. Teeth in groups 1, 2, and 3 had 2mm of gutta percha removed with a warm instrument, then Dycal, Fuki II LC and Z-100 were filled with palstic instruments on the top of the gutta percha respectively. All teeth were bleached for 7 days, fresh bleach was added for another 7 days, then a 10 % methylene blue dye was placed inside the access preparation. They were stored at $37^{\circ}C$ and $100^{\circ}C$ humidity for 5 days. Each tooth was sectioned perpendicular to the long axis using a diamond disk. Initial cuts were made at the most coronal level of facial and lingual CEJ's, then another cuts continued appically in the levels of 0.5mm, 1.5mm, and 2.0mm respectively. The amount of dye leakage through the dentinal tubules was determined at each cut section. In addition, when the cut specimen was determined to be last penetration of any dye, this level was recorded as depth of apical leakage from the coronal terminus of the gutta percha, Dycal, Fuji II LC and Z-100. The acquired data were analyzed by Tukey's Multiple Range Test adn Cochran-Mantel-Haenszel Test to see if there was any statistically significant difference in dye penetration and linear apical leakage among the groups. The results were as follows : 1. Control group at levels of CEJ and 0.5mm, group 3 at level of 1.5mm, and group 2 AND 3 at level of 2.0mm showed the least dye penetration through the facial or lingual dentinal tubules, but there were no significant difference among three groups. 2. Group 2 at levels of CEJ and 0.5mm, group 3 at level of 1.5mm, and group 2 and 3 at level of 2.0mm showed the least dye penetration through the proximal dentinal tubules, but there were no significant difference among control group, group 2, and group 3. 3. Group 1 showed the greatest dye penetration through the facial or lingual and proximal dentinal tubules at all levels, and there were significant difference with other three groups. 4. Control group and group 1 showed 2mm apical dye leakage at facial or lingual and proximal aspects, group 2 showed 1.5mm, and group 3 showed 0.5mm.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.11a
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• pp.378-379
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• 2008

Recently, composite hollow bushings have been increasingly employed mainly from the various characteristics. Composite bushings are superior to porcelain bushings in several respects, including lighter weight, better anti-pollution and anti-explosion properties, and easer manufacturing. Filament wound GRP tubes which have various winding angle were manufactured by using a filament winding machine. This paper will show some design issues and winding condition for composite bushing. And, results show that the winding condition of composite GRP tubes can be used to improved their bending strength and pressure, For bending and pressure tested, tubes with the hybrid winding pattern show higher strength than those of unit winding pattern. Also, the influence of absorption was evaluated through such as measurement of the dye penetration test and water diffusion test, also aspects of surface state using scanning electron microscopy.

• The Journal of the Korean dental association
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• v.15 no.11
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• pp.757-761
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• 1977

The purpose of this study was to evaluate the marginal sealing ability of Hipol composite resin. Using freshly extracted human teeth and 2% acquous methylene blue, the marginal leakage of dye in restorative materials such as Hi-pol, Adaptic, Nuvasystem, Epolite and Amalgam was investigated at 37℃ and under temperature cycling between 4℃ and 60℃. The results were as follows; 1. All the filling materials revealed the penetration of dye between cavity walls and restorations. 2. Hi-pol and Adaptic showed more marginal leakage than other materials and the degree of dye penetration in Hi-pol was similar to that of Adaptic. 3. Nuva system showed the least marginal leakage at 37℃ and under temperature cycling between 4℃ and 60℃. 4. Under temperature cycling, all materials showed a slight increase in marginal leakage except Epolite, which showed the greastest change in leakage.

• The Journal of the Korean dental association
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• v.16 no.12 s.115
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• pp.935-940
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• 1978

The purpose of this study was to evaluate the marginal sealing ability of 'Hi-Pol' Enamel Bond system. Using freshly extracted human teeth and 2% acquous methylene blue, the marginal leakage of dye in restorative materials such as 'Hi-Pol' Enamel Bond system, Hi-Pol, Adaptic, Nuva system, Epolite and Amalgam were investigated at 37℃ and under temperature cycling in range of 4℃-60℃. the results as follows; 1. All filling materials showed some degree of marginal penetration by 2% methylene blue dye. 2. 'Hi-Pol' Enamel Bond system revealed the least marginal penetration by 2% methylene blue as Nuva did at 37℃, but under temperature cycling in range of 4℃-60℃ it showed the greatest marginal penetration. 3. Hi-Pol restoration showed a tendency the similar to the other composite restoration. 4. Nuva system had the most effective marginal sealing ability either at 37℃ and under temperature cycling.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.31 no.2
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• pp.173-183
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• 2021

Objectives: This study evaluated the skin permeability of lawsone in henna hair dyes to understand the exposure characteristics of henna hair dyes in the human body. It examined the protective effects of protectants by applying protectants A, B, and C to test skin. Methods: Skin absorption tests were conducted using Franz diffusion cells according to OECD test guideline 428. After applying one kind of natural henna hair dye and chemical henna hair dye, respectively, to a standardized pig skin model, samples of receptor fluid were collected at 1h, 3h, 6h, and 24h. The skin permeation of lawsone was determined using HPLC. After the skin absorption experiment, the skin to which hair dye was applied was analyzed to determine the residual amount of lawsone in the skin. Results: The cumulative permeation of both natural and chemical henna hair dyes increased over time, and the natural henna hair dye had a flux value (t=3.194, p<.05) high both in the K_p value (t=3.207, p<.05) and the residual amount (t=22.701, p<.001). For skin treated with a protectant, the cumulative permeation of natural henna hair dye 24h control and the cumulative permeation of protectant A, B, and C increased over time. Flux and K_p values were in the order control > protectant A > protectant C > protectant B. The residual amount (F=4.469, p<.05) was in the order of protectant C > protectant A > protectant B > control. At 3h, the dye application time of natural henna hair dye, the lawsone flux value (F=4.454, p<.05) and K_p value (F=4.455, p<.05) were higher in the control group than in the protectant groups. The 24h cumulative permeation of the chemical henna hair dye increased with time in both the control and the protectant groups, and the flux and K_p values were in the order of protectant A > protectant C > protectant B > control. The residual amount (F=7.901, p<.01) was in the order of protectant B > protectant A> protectant C > control. Conclusions: Within the normal dyeing time for henna hair dye (three hours for natural henna hair dyes and 30 minutes for chemical henna hair dyes) lawsone skin penetration was not observed even when no protective agent was applied. After that time, however, evidence of skin penetration and retention of lawsone and the protective effect of protective agents were observed.