• Journal of Embryo Transfer
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• v.12 no.3
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• pp.307-313
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• 1997

Experiments were conducted to assess the effect of quality and viability of bovine blastocysts derived from in-vitro culture(IVC) of in vitro matured and fertilized(IVM-IVF) oocytes during their transport 2 hours. Follicular oocytes were collected form ovaries obtained at a slaughterhouse and were cultured for 24 hours in TCM-199. The IVM oocytes were fertilized in vitro with caudal epididymis spermatozoa. Fertilized oocytes were cultured for 7 to 9 days, and embryos that developed to the blastocyst stage were used for the experiment. The blastocysts, packed in straws with storage medium that consisted TCM-199 with HEPES equilibratd in air and supplemented with 10% FCS were transported at 39~(2.0 h). The quality of blastocysts was assessed and ranked as A(excel-lent), B(Good), fair or poor after transportation. The percentages of A and B grade blastocysts after transport duration for < 1 hours(97.7%) were similar to the result from transport duration for 1~2 hours (92.9%) and 2~3 hours(89.6%), but significantly(P<0.05) higher than transpot duration for 3~4 hours(76.3%). The percentages of A and B grade blastocysts after transport duration for two hours from developed blastocyst at 7day(100%) and 8day(85.0%) were higher 9day(96.6%) and >9day (40.0%). And early to expanded blastocyst produced in vitro were transferred to recipient cow by additional embryos at 7 and 8th day after AI. Three of them were pregnant to term and produced four twin calves, and two calves was premature birth. The gestation lengths of male to female and female to female twin were 282 and 281 days, respectively. And birth weight of twin calves were male to female(22.Skg) and female to female twin(20.3Okg), respectively.

• Biotechnology and Bioprocess Engineering:BBE
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• v.4 no.1
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• pp.46-50
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• 1999

The application of pulsed electric field was investigated in the crossflow ultrafiltration of BSA (bovine serum albumn) to economize the application time of electric current as well as to avoid inherent problems of long-term application of electric field. During the application of various cyclic patterns of pulsed electric current, the averaged filtration flowrate and the degree of concentration were maintained higher than those obtained in the absence of electric current application. The temperature increase, pH change, and BSA loss by electrodeposition were all negligible during the operation. The averaged filtration flowrate increased as the ON/OFF duration ratio of electric current was higher and as the period of ON/OFF cycle was shorter. The re-establishment of concentration polarization was dependent to the duration of current OFF state and, therefore, a longer duration of OFF state was not favorable in maintaining higher filtration flow rate. Although the averaged filtration flowrate was enhanced as the magnitude of electric current increased, the flowrate enhancement became smaller as the magnitude of current value above which the degree of electrokinetic depolarization is no further improved.

• Structural Engineering and Mechanics
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• v.49 no.2
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• pp.261-271
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• 2014

This paper reported an experimental study on creep behaviors of PVB and Ionoplast laminated glass (LG) under load duration of 30 days. The tests were carried out in room temperature ($23^{\circ}C$). The study revealed that after sustaining loads for 30 days, the mid-span deflection of PVB LG increased by almost 102% compared with its short term deflection, while that of Ionoplast LG approximately increased by 14%; composite effects between two glass plies in PVB LG gradually reduced with time, but did not fully vanish at the 30th day; two glass plies in Ionoplast LG on the other hand was able to withstand loads as an effective composite section during the entire loading period; the creep behaviors of both LG were not finished yet at the 30th day. In addition to this, also studied was the varying of the bending stresses of PVB and Ionoplast LG under load duration of 2 hours. The tests were carried out in ambient temperatures of $30^{\circ}C$, $50^{\circ}C$ and $80^{\circ}C$ respectively. It was found that under a given load, although the bending stresses of both LG increased with increasing temperature, for PVB LG the increasing rate of the bending stress decreased with increasing temperature, while for Ionoplast LG the increasing rate of the bending stress increased with increasing temperature.

• Journal of Korean Society of Forest Science
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• v.97 no.5
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• pp.547-551
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• 2008

The study aimed to develop a cryopreservation method for long-term storage using mature somatic embryo of Japanese larch. In this study, desiccation treatments significantly affected re-induction rates of embryogenic tissue (ET) from dried somatic embryos. In the effect of different dehydration temperature and duration on the re-initiation ET. the highest frequency was shown when somatic embryos were dehydrated at $25^{\circ}C$ for 2 (45.5%) or 1 day (43.3%), respectively. In addition, low temperatures [$4^{\circ}C$, 2 days (44.2%) or 3 days (43.5%)] were marked higher ET initiation. After that, the initiation value was declined with dehydration duration. For comparison of different relative humidity on re-induction frequency of ET, the best re-induction (43.5%) was obtained from somatic embryos pre-dried at $(NH_4)_2SO_4$ (RH 79%). Both $Na_2HPO_4$ (RH 97%) and $Na_2CO_3$ (RH 88%) treatments were showed the similar rate, 34.6, 34.2%, respectively. However the lowest rate (19.6%) was observed in distilled water (RH 100%). In comparison of the various storage temperatures and duration of the dried somatic embryos, the highest frequency (66.9%) of re-initiation was obtained when somatic embryos were cryopreserved for one day. However, the frequency was gradually decreased as the time length of storage increased regardless of types of storage. None of ET re-initiated when stored at $4^{\circ}C$ for 1, 2 and 84 days.

• Journal of The Korean Society of Agricultural Engineers
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• v.51 no.5
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• pp.107-113
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• 2009

The purpose of this study is to estimate the design flood runoff for ungaged forest watershed to reduce the flood damage in national park. Daewonsa watershed in Jirisan National Park was selected as study watershed, of which characteristic factors were obtained from GIS data. Flood runoff was simulated using SCS unit hydrograph module in HEC-HMS model. SCS Curve Number (CN) was calculated from forest type area weighted average method. Huff's time distribution of second-quartile storm of the Sancheong weather station, which is nearest from study watershed, was used for design flood runoff estimation. Critical storm duration for the study watershed was 3 hrs. Based on the critical duration, the peak runoff for each sub-watershed were simulated. It is recommended to monitor the long-term flow data for major stream stations in National Park for a better reliable peak runoff simulation results.

• International Journal of Concrete Structures and Materials
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• v.4 no.2
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• pp.89-96
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• 2010

This paper focuses on the effect of creep on the critical compressive strain (CCS) of concrete. The strain of concrete corresponding to the peak compressive stress is crucial in the selection of the ultimate yield strength of the reinforcing bar used in reinforced concrete columns. Among the various influencing factors, such as the creep, shrinkage, loading rate and confinement, the effect of creep and shrinkage is the most significant. So far, investigations into how these factors can affect the CCS of concrete have been rare. Therefore, to investigate the effect of creep and shrinkage on CCS, an experimental (part I) and a parametric study (part II) were conducted, as presented in these papers (part I considers creep effect, part II considers effect of creep and shrinkage). In part I, experiments pertaining to the loading age, loading rate, loading duration and loading and creep levels were conducted to study the effect of these variables on the CCS of concrete. It was found that the effects of the loading rate, loading age, and level and duration on the CCS of concrete were negligible. However, it is very important to consider the effect of creep.

• Asian-Australasian Journal of Animal Sciences
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• v.6 no.4
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• pp.619-622
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• 1993

Rumen bacterial amino acids in sheep on urea diet were monitored to assess a possible change in amino acid synthesis as a long term response to high rumen ammonia environment. A sheep was fed a semipurified diet with soybean meal, followed by a diet with urea as a main nitrogen source. Mixed rumen bacteria were harvested from ruminal fluid taken 3 h after feeding (twice in soybean meal feeding and 6 times in urea feeding) and fractionated as cell wall, proteins and protein-free cell supernatant of monitor amino acids in each fraction. Ruminal ammonia concentration at the sampling ranged from 5.7 to 39.5 mgN/dl. Cell wall and protein fractions of mixed rumen bacteria were stable in their amino acid composition regardless of nitrogen sources of diet and the feeding duration. However, protein-free cell supernatant fraction showed a higher alanine proportion with urea feeding (18.6 and 28.2 molar % of alanine for samples from sheep fed soybean meal and urea, respectively) and its duration (20.6 and 32.9 molar % for samples from sheep on urea diet for 1 and 65 days, respectively). Total free amino acid level of bacteria was depressed in the initial period of urea feeding but restored on 65th day of the feeding. These results suggest that an alanine synthesizing system may develop in rumen bacteria as urea feeding becomes longer.

• Journal of Nutrition and Health
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• v.29 no.9
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• pp.1003-1012
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• 1996

We examined the relationship among riboflavin intake, work activity, erythrocyte glutathione reductase activity coefficient(EGR AC)and urinary riboflavin excretion. We also attempted to determine factors affecting seasonal riboflavin status of rural women. All information about nutrient intake, work activity and riboflavin biochemical status was repeatly collected in three seasons ; farming season(June), harvest season(October), nonfarming season(February). EGR AC was negatively correlated with riboflavin intake(P<0.005) and positively correlated with the duration(min) of farming activity(P<0.005) and the percentage of lean body mass(LBM) (%) representing long term physical activity(P<0.05) in harvestseason. Urinary riboflavin excretion was positively correlated with the ratio of riboflavin intake to 1,000kcal of energy expenditure (P<0.05) in farming season and negatively correlated with the duration(min) of farming activity (P<0.05) and crude nitrogen balance(P<0.005) in harvest season. It appeared that EGR AC seems to increase and urinary riboflavin excretion seems to decrease as work activity increase. Therefore work activity would be expected to deteriorate riboflavin status. Multiple regression analysis of variables showed that in general EGR AC was affected by riboflavin and energy intakes, energy expenditure, energy balance, the duration(min) of farming activity, LBM (%). Urinary riboflavin excretion was affected by riboflavin and protein intakes, LBM(kg) and crude nitrogen balance. Crude nitrogen balance affected urinary riboflavin excretion in all seasons. The result indicated that work activity as well as nutrient intake seemed to affect riboflavin status, especially EGR AC was affected preferentially be work activity in all seasons.

• Journal of Forest and Environmental Science
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• v.29 no.4
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• pp.307-313
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• 2013

Forest Survey consists of road survey, topographic survey, tree mapping survey, stream survey and also ridge survey. Information from forest survey is important and become essential in preparing base map to be used for forest harvesting planning and control. With the current technologies technique of data processing and mapping from traditionally hand drawn method had shifted to a computer system particularly the use of Computer Aided Design (CAD). This gives great advantages to the forest managers and logging operators. However data processing and mapping duration could be further reduced by integrating CAD with other established software such as Microsoft Excel. This time study to show that there is significance difference in term of duration for data processing and efficiency using AutoCAD in combination with Microsoft Excel program as compare to the use of AutoCAD program alone. From the study, it shows that the integration of AutoCAD and Microsoft Excel is able to reduce 70% of duration for data processing and mapping as compared to the use of AutoCAD program alone.

• Korean Institute of Interior Design Journal
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• v.22 no.1
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• pp.283-290
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• 2013

The change of shopping environment created new emerging type of shopping center after 1990's, Urban Entertainment Center(UEC) in northen America. One hand traditional shopping center was retail-centered, the other hand UEC is entertainment-centered and offers the trinity of synergy. Each components, that are retail, dining and entertainment, play a role of drawing people, extending duration of visiting and making people revisit then the synergy makes commercial profit in shopping center. As northen America many of shopping centers with complex have been built in Korea since 2000 and some projects is planning by the change of shopping environment and regenerating urban. Although the term of "UEC" is used in Korea, it seems to be added entertainment facilities to shopping center without considering on commercial strategy. This study will take a look at mix and duration of visit which ULI stresses in UEC development and comparing with those of Yongsan Station and Chyeongrangri Station which are built recently in seoul, it will get characters and situation of these UECs. Finally, the analysis is to be used as a planning data in UEC development.