• Asian-Australasian Journal of Animal Sciences
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• v.30 no.2
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• pp.236-245
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• 2017

Objective: The study was to investigate the effects of alanyl-glutamine (Ala-Gln) and glutamine (Gln) supplementation on the intestinal mucosa barrier in piglets. Methods: A total of 180 barrows with initial weight $10.01{\pm}0.03kg$ were randomly allocated to three treatments, and each treatment consisted of three pens and twenty pigs per pen. The piglets of three groups were fed with control diet [0.62% alanine (Ala)], Ala-Gln diet (0.5% Ala-Gln), Gln diet (0.34% Gln and 0.21% Ala), respectively. Results: The results showed that in comparison with control diet, dietary Ala-Gln supplementation increased the height of villi in duodenum and jejunum (p<0.05), Gln supplementation increased the villi height of jejunum (p<0.05), Ala-Gln supplementation up-regulated the mRNA expressions of epidermal growth factor receptor and insulin-like growth factor 1 receptor in jejunal mucosa (p<0.05), raised the mRNA expressions of Claudin-1, Occludin, zonula occludens protein-1 (ZO-1) and the protein levels of Occludin, ZO-1 in jejunal mucosa (p<0.05), Ala-Gln supplementation enlarged the number of goblet cells in duodenal and ileal epithelium (p<0.05), Gln increased the number of goblet cells in duodenal epithelium (p<0.05) and Ala-Gln supplementation improved the concentrations of secretory immunoglobulin A and immunoglobulin G in the jejunal mucosa (p<0.05). Conclusion: These results demonstrated that dietary Ala-Gln supplementation could maintain the integrity of small intestine and promote the functions of intestinal mucosa barriers in piglets.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.5
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• pp.433-441
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• 1995

A series of experiments was conducted to determine the influence of various pepsin-HCL pretreatment factor, hereby the factors of duration of washing for the retrieved bags, inherent to the mobile nylon bag technique (MNBT), on apparent ileal digestibility of crude protein (AIDCP) and apparent ileal digestibility of dry matter (AIDDM). At last, the AIDCP and apparent ileal digestibility of amino acids (AIDAA) in maize, barley, wheat, rapeseed meal, cottonseed meal and three mixed diets were determined with the MNBT and ileo-rectal anastomis pigs (IRAT). For the MNBT techniques, bag measuring $25{\times}40$ MM and containing 0.75 g feedstuff samples, after pre-digestion in vitro, were introduced into the ileo-rectal anastomis pigs (IRAT) gastrointestinal tract through a duodenal cannula and recovered in the ileal digesta between 6 and 12 h. later. 1. The apparent ileal digestibility of dry matter (AIDDM) and crude protein (AIDCP) of the tested samples, with the exception of fish meal, determined by MNBT were not affected by the different pepsin-HCL pretreatment times in vitro between 2.5 h. and 4 h. 2. There was no significant (p > 0.05) difference of the AIDCP and AIDDM of maize determined by the MNBT among different pepsin concentration (0.03%, 0.07% and 0.1 %) treatment in vitro. 3. The AIDCP determined with the MNBT was affected by the washed and unwashed recovered bags from the ileal digesta. 4. The AIDCP and AID amino acids (AIDAA) of maize, barley, wheat, rapeseed meal, soya-bean meal, cottonseed meal and three mixed diets from the MNBT, with a solution of 0.01N HCL (PH 2) and 0.1% of pepsin concentration, a pepsin-HCL pretreatment time in vitro or 4h. and a washing time of the recovered bag from the ileal digesta compared well with those from the IRAT. The linear regression analysis showed a significant correlation (p < 0.01) of AIDCP and AIDDA between the IRAT and MNBT.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.5
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• pp.510-521
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• 1998

The effects of two types of protein, soybean meal (SBM) and fish meal (FM); and two types of energy supplements, corn flour (CF) and paper pulp (PP), on intake of guinea grass (Panicum maximum), fibre digestion and microbial activities in four Merino rams with an average weight of $54.4{\pm}4.5kg$ were studied. Each animal was fitted with a ruminal cannula and a duodenal cannula at the proximal position. The animals were fed twice daily with chopped guinea grass (5 cm) ad libitum and one of the four dietary supplements: 170 g FM+268 g PP; 170 g FM+268 g CF; 200 g SBM+200 g PP or 200 g SBM+200 g CF. All the supplements were mixed with 100 g molasses. In sacco and in vivo digestibilities, digesta flow rates, fermentation and microbial population were studied in a $4{\times}4$ Latin square design with a $2{\times}2$ factorial arrangement of dietary treatments. The effects of energy or protein sources were not significant on grass intake of sheep. The potential degradabilities of NDF and ADF were not significantly affected by any of the supplements. However, the energy and protein sources had significant efects on disappearance rate of NDF and ADF. The disappearance rate of both NDF and ADF were significantly (p < 0.05) higher in animals fed PP when compared to animals fed CF. Animals fed FM also showed significantly (p < 0.03) higher disappearance rate of ADF than those fed SBM. Animals fed PP showed better digestion in the rumen and total tract. Total flow of NDF and ADF through the duodenum was not significantly affected by the various supplements. The mean rumen pH values (5.8-6.1) were not significantly different among the four different diets. The concentration of rumen ammonia was significantly (p < 0.0001) higher in animals fed SBM (235-266.4 mg N/L) supplement than in animals fed FM (174.9-179.7 mg N/L), while total VFA concentration was not significantly affected by both energy and protein supplements. Mean values of total VFA ranged from 72.5-82.3 mM. Molar proportions of acetate, propionate and butyrate were typical of a roughage type fermentation. Molar proportion of acetate was significantly (p < 0.0001) higher in sheep fed PP when compared to sheep fed CF. Animals fed FM had higher total viable bacterial counts, while animals fed CF showed higher protozoal numbers. Proportions of cellulolytic bacteria were only slightly higher in animals fed SBM or PP.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.1
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• pp.54-60
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• 2001

A study was conducted to assess the nutritive value of two diets based on a low-energy variety of wheat, RAC C1 and their effects on intestinal mucosal structure and function in broiler chickens. The diets were fed with or without microbial enzyme supplement to male and female broiler chickens. The digesta viscosity was reduced (p<0.001) through supplementation with a microbial enzyme in male and female chicks. Enzyme supplementation also improved the dietary apparent metabolizable energy content (p<0.001) and had slight but non-significant positive effects on chick growth and feed conversion ratio. Intestinal mucosal structure and enzyme function were not affected by microbial enzyme supplement. Male chicks consumed more feeds (p<0.001), attained higher final body weight (p<0.001) and were more efficient at feed utilization (p<0.01) than the female chicks. Except for duodenal villus surface area and ileal protein content, intestinal mucosal structure and enzyme activities were similar between the two sexes and dietary treatment groups. The study showed an improvement in the nutritive value of the diets in the presence of the microbial enzyme supplement.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.1
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• pp.38-44
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• 2002

Nine crossbred castrated lambs fitted with rumen and duodenum cannula and fed a diet of hay and concentrate formulated with ground corn and soybean meal as main ingredients were used to assess the duodenal ideal amino acid pattern. Three synthetic amino acid mixtures with different profile of essential amino acids were duodenally infused in order to get three different amino acid patterns flowing into the duodenum. The mixtures were designed to have similar amino acid profile as rumen microbial protein (Pm), casein (Pc) and modified muscle amino acid (Pmm). Results showed a lower urine nitrogen excretion (p=0.05), a higher nitrogen retention (p=0.04) and bodyweight gain with treatment Pmm. The modified muscle amino acid pattern also promoted a lower ratio of Gly to other amino acids in plasma (Gly/OAA) and a higher RNA and RNA/DNA concentration in the liver of the sheep. Meanwhile, the urea concentration in plasma was reduced and the insulin concentration was increased with Pmm treatment. No differences in glucose and growth hormone concentration in plasma were found among three treatments. All results obtained indicate that the modified muscle amino acid pattern (Lys 100%, Met+Cys 39%, Thr 76%, His 41%, Arg 72%, Leu 158%, Ile 81%, Val 105%, Phe 81% and Trp 13%) was the best for growing sheep.

• Biomolecules & Therapeutics
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• v.17 no.2
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• pp.125-132
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• 2009

Calbindin-$D_{9k}$ (CaBP-9k), a cytosolic calcium-binding protein, is expressed in a variety of tissues, i.e., the duodenum, uterus, placenta, kidney and pituitary gland. Duodenal CaBP-9k is involved in intestinal calcium absorption, and is regulated at transcriptional and post-transcriptional levels by 1,25-dihydroxyvitamin D3, the hormonal form of vitamin D, and glucocorticoids (GCs). Uterine CaBP-9k has been implicated in the regulation of myometrial action(s) through modulation of intracellular calcium, and steroid hormones appear to be the main regulators in its uterine and placental regulation. Because phenotypes of CaBP-9k-null mice appear to be normal, other calcium-transporter genes may compensate for its gene deletion and physiological function in knockout mice. Previous studies indicate that CaBP-9k may be controlled in a tissue-specific fashion. In this review, we summarize the current information on calcium homeostasis related to CaBP-9k gene regulation by GCs, vitamin D and its receptors, and its molecular regulatory mechanism. In addition, we present related data from our current research.

• Journal of Ginseng Research
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• v.47 no.4
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• pp.572-582
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• 2023

Background: Free fatty acid-induced lipotoxicity is considered to play an important role in pancreatic β-cell dysfunction. The effect of ginsenosides on palmitic acid-induced pancreatic beta-cells cell death and failure of glucose-stimulated secretion of insulin (GSIS) was evaluated in this study. Methods: Enzyme-linked immunosorbent assay kit for a rat insulin was used to quantify glucose-stimulated insulin secretion. Protein expression was examined by western blotting analysis. Nuclear condensation was measured by staining with Hoechst 33342 stain. Apoptotic cell death was assessed by staining with Annexin V. Oil Red O staining was used to measure lipid accumulation. Results: We screened ginsenosides to prevent palmitic acid-induced cell death and impairment of GSIS in INS-1 pancreatic β-cells and identified protopanaxadiol (PPD) as a potential therapeutic agent. The protection effect of PPD was likely due to a reduction in apoptosis and lipid accumulation. PPD attenuated the palmitic acid-induced increase in the levels of B-cell lymphoma-2-associated X/B-cell lymphoma 2, poly (ADP-ribose) polymerase and cleaved caspase-3. Moreover, PPD prevented palmitic acid-induced impairment of insulin secretion, which was accompanied by an increase in the activation of phosphatidylinositol 3-kinase, peroxisome proliferator-activated receptor γ, insulin receptor substrate-2, serine-threonine kinase, and pancreatic and duodenal homeobox-1. Conclusion: Our results suggest that the protective effect of PPD on lipotoxicity and lipid accumulation induced by palmitic acid in pancreatic β-cells.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.10
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• pp.1495-1500
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• 2003

Two bio-assays were conducted to evaluate turmeric root powder and mannan-oligosaccharides (MOS) as alternatives to feed antibiotics for broilers. In one trial, one hundred and eighty 19-days old broilers assigned to 18 groups of 10 were fed on one of six experimental diets with three replicates during four weeks. The diets included a basal feed without additives and with either virginiamycin, MOS, or turmeric at 1, 2 and 3 g/kg, respectively. In the second trial, one hundred and forty four 21-days old broilers arranged in 16 groups of nine were fed on the first four diets with four replicates for a similar period. Virginiamycin, MOS and turmeric (1 g/kg) in the first trial generally improved the weight gain of broilers by 3.4, 6.2 and 5.3%, respectively. In the second trial they increased the weight gain significantly (p<0.05) by 8.8, 8.0 and 15.1%, respectively. Additives improved the feed efficiency up to 15.1% and carcass recovery up to 3.1% (p<0.05). Virginiamycin, MOS and turmeric (1 g/kg) markedly reduced the abdominal fat content from 1.91% BW in the control to 1.44, 0.97 and 1.2% BW, respectively, in the first trial. The corresponding values obtained in the second trial were 1.01, 0.55 and 0.6%, respectively as compared to 1.22% in the control group. All additives showed a remarkable inhibition of duodenal coliform bacteria, yeast and mould in the caecum, and all viable microbes in the ileum. A significant (p<0.05) improvement in energy and protein utilization could be recorded with supplemented diets except for high turmeric diets. Dietary 2 and 3 g/kg addition of turmeric reduced energy and protein utilization as well as fat deposition. Present results reveal that turmeric and MOS are satisfactory alternatives to antibiotics in broiler feeds. Both MOS and turmeric possess an antimicrobial effect in vivo. Turmeric may also depress fat deposition in broilers.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.48
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• pp.58-64
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• 2003

To enjoy a healthy life, it is important to have a well-balanced diet. However, in today's society, there is an increase in the consumption of preprocessed foods and frequency of eating out. Also the western diet, which is becoming move popular worldwide, contains relatively high levels of protein and fat, and a low amount of fiber, Furthermore, the increased availability of favorite foods has created a condition were the individual diet is less variable. With these conditions, it is difficult to maintain a diet that is nutritionally balanced. With these unbalanced diets, which are difficult to change, there has been an increase in adult disease and health problems, such as colon and breast cancer, It is speculated that metabolites for carcinogens are produced from diet components and that intestinal bacteria contribute to the production of these metabolites. Therefore, it is necessary to evaluate the relationships between health, diet, and intestinal microflora. Soybean oligosaccharide is composed of water-soluble saccharides that have been extracted from soybean whey, a by-product from the production of soy protein. This is mainly a mixture of mono-, di-, tri-, and tetrasac-charides, with the principle components being the oligosaccharide raffinose and stachyose. When consumed by humans, the oligosaccharides cannot be digested in the human duodenal and small intestinal mucosa, and these are selectively utilized by beneficial bifidobacteria in intestines. The results of acute and subacute toxicity tests, soy-bean oligosaccharides were nonpoisonous. Soybean oligosaccharides promote the growth of indigenous bifido-bacteria in the colon which by their antagonistic effects, suppress the activity of putrefactive bacteria. Also, they reduce toxic metabolites, detrimental enzymes and plasma lipid, and increase in the frequency of bowel evacuation and fecal quantities. Consequently, soybean oligosaccharides as functional foods components have potential roles in the prevention and medical treatment of chronic adult diseases. The study of processing property and physiological function of soybean oligosacchavides and development of high oligosaccharide variety allow the creation of new and exciting foodstuffs that aye functional healthy.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.12
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• pp.1620-1624
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• 2009

This study aimed to clarify the effect of mobile bag size and ratio of sample size to bag surface area on intestinal digestibility of forage in sheep. Four Suffolk ewes fitted with ruminal and proximal duodenal cannulae were fed second-cut Italian ryegrass (Lolium multiflorum Lam.) hay twice daily, and the same forage was used to measure intestinal digestibility. The forage samples were incubated in the rumen for 16 h and then in pepsin-HCl solution for 3 h before intestinal incubation. The incubated forage samples were placed in a nylon mobile bag. The bag sizes used were either 20 mm${\times}$20 mm (small bag size; SBS) or 30 mm${\times}$30 mm (large bag size; LBS) and the ratio of the sample size to the surface area of the bag was either 5.5 $mg/cm^{2}$ (low ratio; LR) or 11.0 $mg/cm^{2}$ (high ratio; HR) resulting in four different treatment conditions: SBS-LR, SBS-HR, LBS-LR and LBS-HR. Eight bags per animal were inserted through the duodenal cannulae at 15-min intervals and were subsequently collected from the feces of the animal. The mean intestinal bag transition time did not differ significantly between animals, but ranged from 23.2 to 27.0 h. The intestinal digestibility of dry matter (IDDM) ranged from 0.162${\pm}$0.019 g/g in the SBS-HR treatment group to 0.195${\pm}$0.018 g/g in the SBS-LR treatment. The intestinal digestibility of crude protein (IDCP) ranged from 0.610${\pm}$0.031 g/g in the LBS-LR treatment to 0.693${\pm}$0.018 g/g in the SBS-LR treatment. There was no difference in the IDDM and IDCP between different treatments. It was therefore concluded that the size of the mobile bag and the ratio of the sample size to the bag surface area did not influence the intestinal digestibility of forage. Future studies should use bags with high ratios of sample size to surface area in order to obtain sufficient residue for further analysis.