• The Korean Journal of Cytopathology
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• v.15 no.1
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• pp.52-55
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• 2004

Secretory meningioma is a distinct subtype of meningioma. We describe the cytologic features of a secretory meningioma on squash preparations, in comparision with other cytologic mimickers. A 54-year-old woman presented with hearing loss, vertigo, tinnitus, and headache for seven years. A brain MRI study revealed a 4.5cm sized mass in the cerebellopontine angle, which showed homogenous signal intensity in T2-weighted image. The intraoperative squash smear showed some well-defined, thin rimmed intracytoplasmic inclusions, containing a finely granular eosinophilic core among less cohesive meningiomatous cells. Histologic sections revealed a meningothelial meningioma with scattered inclusions, with periodic acid-Schiff, carcinoembryonic antigen, and cytokeratin positivity. Identification of characteristic intracytoplasmic inclusions is helpful for diagnosing secretory meningiomas. On squash preparations, differential diagnoses included tumors with inclusions or cytoplasmic vacuolizations, such as metastatic mammary infiltrating ductal carcinoma, gastric adenocarcinoma, hepatocellular carcinoma, and clear cell ependymoma, oligodendroglioma, hemangioblastoma, chordoma, and other variants of meningiomas (clear cell, xanthomatous, microcytic, and chordoid variants). In addition, the possibilities of glioma with eosinophilic granular body, and metastatic tumors from mammary infiltrating ductal carcinoma, gastric adenocarcinoma, and hepatocellular carcinoma in meningioma should be considered.

• Proceedings of the Korean Information Science Society Conference
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• 2001.10b
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• pp.340-342
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• 2001

본 논문은 유방질환 중에서 Duct(관)에 발생하는 유방 종양을 benign(양성종양)/DCIS (Ductal Carcinoma In Situ)/NOS(Invasive ductal carcinoma)로 자동 분류하기 위한 분류방법을 제안한다. 분류기 생성에서 가장 중요한 단계인 특징 추출단계에서는 wavelet 변환을 적용하였으며, wavelet 변환의 각 depth에 따라 분류기를 생성하여, depth와 생성된 분류기의 분류 정확도와의 상관관계를 비교.분석하였다. 현미경 100배 배율과 400배 배율의 유방 질환 영상을 1, 2, 3, 4단계(depth)의 wavelet 변환을 적용한 후, 분할된 서브밴드에서 GLCM을 이용하여 질감 특징(Entropy, Energy, Contrast, Homogeneity)을 추출하여, 이 특징값들을 조합하여 판별분석에 의해 분류기(classifier)를 생성한 후, 분류 정확도를 검증하였다. Benign/DCIS/NOS를 분류하려면 최소 3단계 이상의 wavelet 변환을 적용해야 하고, 400배 배율 영상보다는 100배 배율의 영상이 더 나은 결과를 보였다.

• Journal of the Institute of Convergence Signal Processing
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• v.2 no.4
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• pp.22-30
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• 2001

In this paper we propose three classification algorithms to classify breast tumors that occur in duct into Benign, DCIS(ductal carcinoma in situ) NOS(invasive ductal carcinoma) The general approach for a creating classifier is composed of 2 steps: feature extraction and classification Above all feature extraction for a good classifier is very significance, because the classification performance depends on the extracted features, Therefore in the feature extraction step, we extracted morphology features describing the size of nuclei and texture features The internal structures of the tumor are reflected from wavelet transformed images with 10$\times$ and 40$\times$ magnification. Pariticulary to find the correlation between correct classification rates and wavelet depths we applied 1, 2, 3 and 4-level wavelet transforms to the images and extracted texture feature from the transformed images The morphology features used are area, perimeter, width of X axis width of Y axis and circularity The texture features used are entropy energy contrast and homogeneity. In the classification step, we created three classifiers from each of extracted features using discriminant analysis The first classifier was made by morphology features. The second and the third classifiers were made by texture features of wavelet transformed images with 10$\times$ and 40$\times$ magnification. Finally we analyzed and compared the correct classification rate of the three classifiers. In this study, we found that the best classifier was made by texture features of 3-level wavelet transformed images.

• Radiation Oncology Journal
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• v.22 no.1
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• pp.11-16
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• 2004

Purpose: To evaluate the survival rate, local failure rate and patterns of failure, and analyze the prognostic factors affecting local relapse of ductal carcinoma in situ treated with breast conserving surgery and radiotherapy Materials and Methods: From June 1995 to December 2001, 96 patients with ductal carcinoma in situ treated with breast conserving surgery and radiotherapy were retrospectively analyzed. The operations were either local or wide excision in all patients, with an axillary lymph node dissection performed in some patients. Radiation dose to the whole breast was 50.4 Gy, over 5 weeks, with 1.8 Gy daily fractions, with additional doses ($10\~14$ Gy) administered to the primary tumor bed in some patients with close ($\leq$2 mm) or positive resection margin. The median follow-up period was 43 months (range $10\~102$ months). Results: The 5-year local relapse free survival and overall survival rates were 91 and $100\%$ respectively. Local relapse occurred in 6 patients ($6.3\%$). Of the 6 recurrences, one was invasive ductal cell carcinoma. With the exception of one, all patients recurred 2 years after surgery. There was no regional recurrence or distant metastasis. Five patients with local recurrence were salvaged with total mastectomy, and are alive with no evidence of disease. One patient with recurrent invasive ductal cell carcinoma will receive salvage treatment. On analysis of the prognostic factors affecting local relapse, none of the factors among the age, status of resection margin, comedo type and nuclear grade affected local relapse. Operation extent also did not affect local control (p=0.30). In the patients with close resection margin, boost irradiation to the primary tumor bed did not affect local control (p=1.0). Conclusions: The survival rate and local control of the patients with ductal carcinoma in situ treated with breast conserving surgery and radiotherapy were excellent. Close resection margin and boost irradiation to the primary tumor bed did not affect local relapse, but further follow-up with much more patients is needed.

• The Korean Journal of Cytopathology
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• v.9 no.1
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• pp.89-94
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• 1998

A case of fine needle aspiration cytology of an osteoclastic giant cell tumor of pancreas, which is an uncommon variant of ductal adenocarcinoma, is described. Aspirated tumor cells were characterized by three populations: (1) bland osteoclast like giant cells with multiple small, round nuclei with distinct nucleoli, and abundant cytoplasm, (2) Individually scattered or loosely clustered medium sized mononuclear tumor cells, having fine chromatin, smooth nuclear membrane, often prominent nucleoli, and high N/C ratio, (3) bland or atypical spindle shaped cells. Osteoid like lacy material was also seen on cell block section. The immunohistochemical studies using paraffin embedded cell block section showed positivities for vimentin and lysozyme in both giant and mononuclear turner cells. However, they were negative for cytokeratin, epithelial membrane antigen, S-100 protein, carcinoembryonic antigen, and p53.

• Archives of Pharmacal Research
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• v.21 no.4
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• pp.398-405
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• 1998

We investigated the effects of triterpene acids (TAs), ursolic acid (UA) and oleanolic acid (OA), on the induction of proliferation and differentiation of normal rat mammary epithelial cells (RMEC) or organoids cultured in Matrigel or primary culture system. To elucidate the effects, we tested their differentiation inducing activities with intercellular communication ability, cell cycle patterns, induction of apoptosis, and morphological differentiation in the three dimensional extracellular culture system. To study the changes of RMEC subpopulation in culture, the cultured cells were isolated, immunostained with peanut lectin (PNA) and anti-Thy-1.1 antibody and then analyzed with flow cytometry. Four different subpopulations, such as PNA and Thy-1.1 negative cells (B-), PNA positive cells (PNA+), Thy-1.1 positive cells (Thy-1.1+), PNA and Thy-1.1 positive cells (B+), were obtained and the size of each subpopulation was changed in culture with time in the presence of TAs. Intercellular communication was observed in culture for 7 days in TAs-treated cells, but not in culture for 4 days with scrape-loading dye transfer technique. $G_2$/M phase cells and the number of apoptotic population were increased in TAs-treated groups in cell cycle analyses. S phase fractions were reduced and the change of $G_1$ phase cells was not observed. The colonies with distinct multicelfular structures, such as stellate, ductal, webbed, squamous, lobulo-ductal colonies, were observed in Matrigel culture and the frequencies of each colony were changed in the presence of TAs. These results suggest that UA and OA have differentiation inducing effects on rat mammary epithelial cells in primary or in Matrigel culture.

• Clinical Endoscopy
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• v.56 no.3
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• pp.353-366
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• 2023

Background/Aims: This study aimed to clarify the efficacy and safety of pancreatic duct lavage cytology combined with a cell-block method (PLC-CB) for possible pancreatic ductal adenocarcinomas (PDACs). Methods: This study included 41 patients with suspected PDACs who underwent PLC-CB mainly because they were unfit for undergoing endoscopic ultrasonography-guided fine needle aspiration. A 6-Fr double lumen catheter was mainly used to perform PLC-CB. Final diagnoses were obtained from the findings of resected specimens or clinical outcomes during surveillance after PLC-CB. Results: Histocytological evaluations using PLC-CB were performed in 87.8% (36/41) of the patients. For 31 of the 36 patients, final diagnoses (invasive PDAC, 12; pancreatic carcinoma in situ, 5; benignancy, 14) were made, and the remaining five patients were excluded due to lack of surveillance periods after PLC-CB. For 31 patients, the sensitivity, specificity, and accuracy of PLC-CB for detecting malignancy were 94.1%, 100%, and 96.8%, respectively. In addition, they were 87.5%, 100%, and 94.1%, respectively, in 17 patients without pancreatic masses detectable using endoscopic ultrasonography. Four patients developed postprocedural pancreatitis, which improved with conservative therapy. Conclusions: PLC-CB has an excellent ability to detect malignancies in patients with possible PDACs, including pancreatic carcinoma in situ.

• Journal of Oral Medicine and Pain
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• v.37 no.2
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• pp.81-91
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• 2012

It has been known that saliva may affect the most of oral diseases. On the contrary, several systemic conditions may affect salivary flow and cause oral dryness and psychosocial stress especially may a crucial role in the etiology of hyposalivation and oral dryness. Many studies have focused on macroscopic effects of the stress on the salivary glands by autonomic respose, but on the other hand it has hardly been reported on cellular microscopic effects of the stress on the salivary glands. Therefore, this study was performed to examine clusterin, a antiapoptotic and cytoprotective protein, in the parotid glands under restraint stress condition. For this study, 10 rats were divided into 3 groups; 1) 2 rats of group I were selected as a normal control. 2) 2 rats of group II, as a experimental control were placed in the restraint cone for 2 hours 3) 6 rats of group III were placed in the restraint cone for 2 hours once a day. The rats were sacrificed immediately(group II, as a experimental control), 24, 48, and 72 hours after application of the stress and the parotid glands were excised. Western blotting and immunohistochemistry were performed. The finding were as follows: 1. In parotid glands, clusterin was mildly increased and clearly expressed in the ductal cell under restraint stress immediately after application of the stress. 2. In parotid glands, clusterin was significantly decreased and slightly stained in the ductal cell under restraint stress 24 and 48 hours after experiment. 3. In parotid glands, clusterin was prominently increased again and densely stained in the ductal cell under restraint stress 72 hours after experiment.

• Journal of Korean Dental Science
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• v.6 no.1
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• pp.22-26
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• 2013

Purpose: Transglutaminase 2 (TGase 2) is expressed by tumor necrosis factor-${\alpha}$ in various carcinoma. The role of TGase 2 expression in salivary gland tumors is not clear yet. Established slaivary gland tumor (SGT)cell line has been used to study the pathogenesis of salivary gland adenocarcinoma on a cellular level in vitro. The pupose of this study were to examine mRNA expression of TGase 2 in SGT cell line compared to other tumor cell lines, and to apply these results to the pathogenesis of salivary gland tumor. Materials and Methods: After SGT, SCC-15, HN 4, and HeLa tumor cell lines were cultured under preconfl uency, and 3 days after postconfl uency, the cells were harvested for total RNA extraction and cDNA preparation. Result: Reverse transcription polymerase chain reaction for semiquantitative mRNA analysis was done. TGase 2 mRNA expression was not induced by confl uency in all the cell lines. TGase 2 mRNA expression was variable but markedly enhanced in SGT cell line. Conclusion: mRNA expression of TGase 2 should play an important role in the pathogenesis of SGT cell line originated from ductal cell.

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2002.11a
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• pp.9-9
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• 2002

Grossly, the liver exhibits marked cirrhotic changes characteristics of the pre-transformation phase of WHV. Microscopically, focal hepatocyte necrosis and inflammatory cells were observed in midzonal and periportal areas. Bridging portal fibrosis produced pseudolobulation due to entrapment of hyperplastic hepatocytes. Biliary hyperplasia, ductal cell proliferation, and increased amounts of fibrous connective tissue expanded portal areas and extended into periportal areas. Myofibroblasts stained positive for -SMA were detected in proliferating fibrotic tissue and sinusoids.