• Molecules and Cells
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• 제27권6호
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• pp.689-695
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• 2009

Chemically synthesized small interfering RNAs (siRNAs) can specifically knock-down expression of target genes via RNA interference (RNAi) pathway. To date, the length of synthetic siRNA duplex has been strictly maintained less than 30 bp, because an early study suggested that double-stranded RNAs (dsRNAs) longer than 30 bp could not trigger specific gene silencing due to the induction of non-specific antiviral interferon responses. Contrary to the current belief, here we show that synthetic dsRNA as long as 38 bp can result in specific target gene silencing without non-specific antiviral responses. Using this longer duplex structure, we have generated dsRNAs, which can simultaneously knock-down expression of two target genes (termed as dual-target siRNAs or dsiRNAs). Our results thus demonstrate the structural flexibility of gene silencing siRNAs, and provide a starting point to construct multifunctional RNA structures. The dsiRNAs could be utilized to develop a novel therapeutic gene silencing strategy against diseases with multiple gene alternations such as viral infection and cancer.

• 한국방사선학회논문지
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• 제17권1호
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• pp.107-114
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• 2023

엑스선형광분석은 비파괴적으로 시료에 포함된 원소와 농도를 분석할 수 있는 기법으로 과학 및 산업 분야에 광범위하게 사용되고 있다. 다양한 물질을 포함한 혼합물 또는 화합물 분석의 정밀도 향상을 위해 10 keV 근방의 저에너지와 20 keV 근방의 에너지 영역에 높은 강도 분포를 갖는 엑스선관이 요구된다. 두 에너지 영역에서 높은 강도 분포를 갖는 스펙트럼을 얻기 위하여 9.65 keV의 특성엑스선을 가지는 텅스텐과 17.48 keV의 몰리브덴 두 물질을 적층한 구조의 표적을 가지는 투과형 엑스선관을 몬테카를로 전산모사를 통해 스펙트럼을 분석하였다. W-Mo 구조의 표적을 통해 10 keV와 20 keV 근방의 강한 강도를 갖는 특성엑스선을 얻었다. 또한 4 ㎛ 두께의 Mo-W multilayers 구조의 표적을 통해 최적의 강도 분포를 갖는 스펙트럼을 얻을 수 있음을 확인하였다. 다양한 표적 물질을 선택 조합하고 두께 최적화를 통해 원하는 에너지 대역에서 높은 강도 분포를 갖는 스펙트럼을 얻는 것이 가능하다.

• 대한기계학회논문집A
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• 제40권8호
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• pp.721-724
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• 2016

실제 사용되는 기계 구조물의 수명 예측은 공정산포, 미래의 하중조건, 손상 모델의 불완전성 등으로 인하여 실험실에서 얻은 결과와 차이를 보일 수 밖에 없다. 본 연구에서는 이러한 불확실성이 내포된 기계 구조물의 피로파괴를 사전에 예지하기 위해 이중센서를 개발하고, 상온에서 단축 피로 하중을 받는 구조물에 적용하여 실증하였다. 이중 센서는 피로하중을 받는 대상 구조물보다 약간 더 큰 응력이 인가되도록 고안된 감지 구조물에 변형률 게이지를 부착한 형태이며, 균열이 센서자체에 먼저 발생함으로써 피로파괴를 예지하며, 쌍으로 제작된 감지부의 변형률 차를 이용하여 변동하중 하에서도 정상상태와 고장상태를 구분이 가능하다.

• KIEE International Transactions on Electrophysics and Applications
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• 제3C권1호
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• pp.23-27
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• 2003

Structural properties of Cu-Ni alloy films, such as preferred orientation, crystallite size, in-ter-planar spacing, cross-sectional morphology, and electrical resistivity, are investigated in terms of tar-get configurations that are used in the film deposition by means of magnetron co-sputtering. Two different target configurations are considered in this study: a dual-type configuration in which two separate tar-gets (Cu and Ni) and different bias types (RF and DC) are used and a Ni-on-Cu type configuration in which Ni chips are attached to a Cu target. The dual-type configuration appears to have some advantages over the Ni-on-Cu type regarding the accurate control of atomic composition of the deposited Cu-Ni alloy. However, the dual-type-produced film exhibits a porous and columnar structure, the relatively large internal stress, and the high electrical resistivity, which are mainly due to the relatively low mobility of adatoms. The affects of thermal treatment and deposition conditions on the structural and electrical properties of dual-type Cu-Ni films are also discussed.

• Asian Pacific Journal of Cancer Prevention
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• 제15권1호
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• pp.111-116
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• 2014

Objective: To study potential targets of Danshensu via dual inverse docking. Method: PharmMapper and idTarget servers were used as tools, and the results were checked with the molecular docking program autodock vina in PyRx 0.8. Result: The disease-related target HRas was rated top, with a pharmacophore model matching well the molecular features of Danshensu. In addition, docking results indicated that the complex was also matched in terms of structure, H-bonds, and hydrophobicity. Conclusion: Dual inverse docking indicates that HRas may be a potential anticancer target of Danshensu. This approach can provide useful information for studying pharmacological effects of agents of interest.

• Smart Structures and Systems
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• 제25권3호
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• pp.301-310
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• 2020

The laser ultrasonic technique is gaining popularity for nondestructive evaluation (NDE) applications because it is a noncontact and couplant-free method and can inspect a target from a remote distance. For the conventional laser ultrasonic techniques, a pulsed laser is often used to generate broadband ultrasonic waves in a target structure. However, for crack detection using nonlinear ultrasonic modulation, it is necessary to generate narrowband ultrasonic waves. In this study, a pulsed laser is shaped into dual-line arrays using a spatial mask and used to simultaneously excite narrowband ultrasonic waves in the target structure at two distinct frequencies. Nonlinear ultrasonic modulation will occur between the two input frequencies when they encounter a fatigue crack existing in the target structure. Then, a nonlinear damage index (DI) is defined as a function of the magnitude of the modulation components and computed over the target structure by taking advantage of laser scanning. Finally, the fatigue crack is detected and localized by visualizing the nonlinear DI over the target structure. Numerical simulations and experimental tests are performed to examine the possibility of generating narrowband ultrasonic waves using the spatial mask. The performance of the proposed fatigue crack localization technique is validated by conducting an experiment with aluminum plates containing real fatigue cracks.

• 한국군사과학기술학회지
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• 제14권3호
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• pp.345-352
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• 2011

This paper focuses on the properties analysis of 9mm bullet dual structure core to substitute current lead core by environment-friendly combination of W-Cu-Ni system high density composite materials. So the four combination samples were made of dual core with the different center of gravity location backward or forward compare to that of lead type bullet, and we experimented about the performance of 9mm bullet dual structure core. In the experimental results, the outer shape of core of four environment friendly samples on the target maintain marginally, while the current lead core bullets are completely crushed after hitting the target. The penetration depth of environment friendly samples excel seven times to lead type bullet and the three out of four samples with forward adjusted center of gravity penetrate deep as twice as ones backward. The impact tolerance of all four samples satisfies military specification, however, more firing tests are required to improve reliability of scattering distribution.

• 한국전자파학회논문지
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• 제18권4호
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• pp.398-407
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• 2007

본 논문에서는 부착될 물질이 가지는 전기적 특성에 의한 성능 변화가 적은 이중 선로의 비대칭 다이폴 형태 RFID 용도 태그 안테나(Asymmetric Dual-arm Dipole Antenna: ADDA)를 제안하였다. 제안된 태그 안테나는 상용 의료 폐기물 수거용 플라스틱 밀폐 용기(${\varepsilon}_r=1.7,\;tan\;{\delta}=0.002$)에 부착하여도 안테나의 동작 특성 변화가 적도록 설계되었다. 안테나의 세부적인 설계변수는 자유공간과 인식 대상 물체에 부착시 우수한 인식거리 성능을 나타내도록 Pareto 유전자 알고리즘을 사용하여 최적화하였다. 최적화 태그 안테나는 $100\;mm{\times}50\;mm$의 크기를 가지며, 3.7 %의 반사 손실 대역폭($S_{11}$< -10 dB)을 가지고 80 %의 복사효율을 나타내었다. 인식 거리 측정 결과, 자유공간에서 정면 방향(broadside) 5.3 m, 의료 폐기물 수거용 플라스틱 밀폐 용기에 부착시 최대 5.5 m의 인식거리 성능을 가지는 것을 확인하였다.

• 천문학회보
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• 제38권2호
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• pp.43.1-43.1
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• 2013

We report the preliminary results of our GMVA(Global mm VLBI Array) observation at the frequency of 86 GHz. Observation were made in the dual polarization mode (LCP and RCP), to produce the polarimetric maps with the maximum angular resolution which the array is capable of. We aim to link the source-integrated AGN polarization properties with the polarized spatial source structure, by mapping the polarized "fine structure" of the target AGN. We selected 2 targets, 0954+658 and 0716+714, which (1) have been observed multiple times by the PdBI polarimetric monitoring program; (2) have sufficient integrated fluxes ($S_{90GHz}$ > 1 Jy) ; (3) are close enough to resolve the source structure < 1 pc with given angular resolution ; and (4) are located at high northern declination for good UV coverages. As preliminary results, we present LL and RR polarized images of each target with the maximum angular resolution of ${\sim}60{\mu}as$. Extended structures, probably the jet outflows, are discovered in both sources.

• BMB Reports
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• 제49권7호
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• pp.355-356
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• 2016

The THO/TREX complex consists of several conserved subunits and is required for mRNA export. In metazoans, THO/TREX binds a subset of mRNAs during RNA splicing, and facilitates their nuclear export. How THO/TREX selects RNA targets is, however, incompletely understood. In our recent study, we reported that THO is loaded onto Piwi-interacting RNA (piRNA) precursor transcripts independent of splicing, and facilitates convergent transcription in Drosophila ovary. The precursors are later processed into mature piRNAs, small noncoding RNAs that silence transposable elements (TEs). We observed that piRNAs originating from dual-strand clusters, where precursors are transcribed from both strands, were specifically affected by THO mutation. Analysis of THO-bound RNAs showed enrichment of dual-strand cluster transcripts. Interestingly, THO loading onto piRNA precursors was dependent on Cutoff (Cuff), which comprises the Rhino-Deadlock-Cutoff (RDC) complex that is recruited to dual-strand clusters by recognizing H3K9me3 and licenses convergent transcription from he cluster. We also found that THO mutation affected transcription from dual-strand clusters. Therefore, we concluded that THO/TREX is recruited to dual-strand piRNA clusters, independent of splicing events, via multi-protein interactions with chromatin structure. Then, it facilitates transcription likely by suppressing premature termination to ensure adequate expression of piRNA precursors.