• Journal of Plant Biotechnology
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• 제32권4호
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• pp.313-319
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• 2005

다양한 탄소원을 사용하였을 시, 세포성장에 미치는 영향은 배양 5일제 glucose를 사용한 배양세포에서 dry cell weight가 11.2 g/L, wet cell weight가 310.8 g/L로 가장 높았다. 이후 배양 10일차에는 오히려 sucrose를 탄소원으로 사용한 배양세포에서 dry cell weight가 13.4 g/L, wet cell weight가 480 g/L로 가장 높이 성장한 것을 확인할 수 있었다. Total secreted protein의 경우는 배양 10일차에 sucrose를 기본 탄소원으로 사용하였을 때 110.3 mg/L로 가장 높게 나왔다. Total secreted protease 역시, 배양 10일차에 sucrose를 기본 탄소원으로 사용한 배양씩에서 3950 U/L로 가장 많은 양이 측정되었다. 최종적으로 재조합된 단백질인 hGM-CSF의 생산량에 대한 측정결과, sucrose를 기본 탄소원로 사용한 배지에서 배양 10일차에 56 mg/L로 가장 높게 측정됨을 확인할 수 있었다. 이 외에 다양한 탄소원 농도변화에 따른 total secreted protein과 hGM-CSF의 안정성 평가를 확인해본 결과, total secreted protein의 경우, 비교대상으로 사용한 sucrose에서는 농도변화에도 큰 손실률을 보이지는 않았으나, sorbitol, mannitol, fructose, glucose의 경우에는 농도가 높아질수록 배양액내의 protein의 손실률이 증가 하였다. 총 분비된 hGM-CSF 역시 sucrose에 비해 sorbitol, mannitol, fructose, glucose에서는 분비된 hGM-CSF의 양이 크게 감소하는 것을 확인할 수 있었고, 탄소원의 농도가 높아질수록 hGM-CSF의 양이 감소하는 경향이 크게 나타났다.

• KSBB Journal
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• 제4권3호
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• pp.271-275
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• 1989

본 논문에서는 Thalictrum rugosum 식물세포의 회분식 배양에 있어서 동역학적 특성들을 연구하였다. 본 실험에서 alkaloid의 생성은 일반적인 2차대사 산물의 생성 특성과 달리 세포성장에 비례함이 확인되었다. 세포 성장기의 비증식속도는 $0.20~0.25\;day\;^{-1}이었으며 FW / DCW 비가 배양상태를 나타내는 중요한 인자임을 알수 있었다. Cell yield는 0.36g cells/g sugar이었으며 berberine은 139mg/L까지 생성되었고 그중 120mg/L는 세포내에 존재하였다. 이를 specific content로 계산하면 dry cell weight의 1.10%가 된다. 세포 성장기에서 비증식속도와 당농도간의 관계는 Monod kinetics로 잘 기술될 수 있었다.

• Preventive Nutrition and Food Science
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• 제11권1호
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• pp.61-66
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• 2006

The objective of this study was to compare the color and microstructure of powder, redness, brownness, and antioxidative activity in extruded ginseng, white ginseng and red ginseng extracts. The colors of extruded dry ginseng powder (moisture content 30%, barrel temperature $110^{\circ}C$, and screw speed 200 rpm) were similar to those of red ginseng. Intact cell wall structure was examined in dried root ginseng at $70^{\circ}C$ (A), white ginseng with skin (D), white ginseng without skin (E), and red ginseng (F) under a scanning electron microscope. The cell wall was not detected in samples B and C (dry ginsengs extruded with 25% and 30% moisture contents, respectively). Intact starch granules were detected in samples A, D, and E under a scanning electron microscope. Melted starch granules were detected in samples B, C, and F. Colors (L, a, b) of 50% EtOH extracts were similar in samples C and F. Browniness and redness of extracts were high in extruded dry ginseng and red ginseng extracts. Extruded dry ginseng (B) showed higher electron donation ability and phenolic content than the other samples.

• 한국수소및신에너지학회논문집
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• 제27권3호
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• pp.264-269
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• 2016

A simple dry chemical approach was developed in order to load palladium (Pd) as a promoter on Pt/gas diffusion electrode (GDE) for polymer electrolyte membrane fuel cell (PEMFC). Palladium(II) bis (acetylacetonate), $Pd(acac)_2$ was sublimed, penetrated into Pt/GDE and then reduced to Pd nanoparticles simultaneously without any reducing agent and any solvent in a glass reactor of $N_2$ atmosphere at $180^{\circ}C$ for 3, 5 and 15 min. Pd distribution was analyzed by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS), and I-V curve was estimated by using a unit cell with $5{\times}5cm^2$ active area.

• KSBB Journal
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• 제14권3호
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• pp.264-272
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• 1999

Mandelate pathway를 거치는 Pseudomonas putida(KCTC 1751)의 전세포 benzoylformate dcarboxylase를 이용하여 benzoylformate를 benzaldehyde로 변환하였고 성장배지의 조성이 cell내부에 축적되는 benzoylformate dcarboxylase의 양에 미치는 영향을 조사하였다. 전세포효소의 재사용을 위하여 calcumalginate 캡슐 고정회법을 이용하여 캡슐고정화 Pseudomonas putida를 제조하였다. 캡슐 고정화 미생물을 M3배지에서 3일간 배양한 후 M1배지에서 1일간 배양한 결과 77.75g/L의 미생물 건조중량을 얻었다. 캡슐 고정화 전세포 benzoyltormate decarboxylase의 비활생도는 자유배양에 의한 전세포효소의 비활성도에 비해 약 1/2값을 나타내었으며 캡슐 고정화 전세포 benzoyltormate decarboxylase를 20회 재사용시 20%의 실활을 보였으며 캡슐 재사용 30회 이후 미생물의 건조중량은 약 10% 감소를 보였다.

• 식물조직배양학회지
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• 제21권2호
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• pp.91-97
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• 1994

고구마 현탁배양세포로부터 POD 고생산세포주로 선발한 SP-47세포주를 사용하여 POD생산성을 향상시키기 위하여 식물생장조절제 및 탄소원의 종류와 농도, 세포접종량 등의 배양조건을 적정화하였다. 30 g/L Surcrose, 1 mg/L 2,4-D가 첨가된 LS배지 50mL을 함유한 30 mL Erlenmeyer flask에 세포생중량 1 g을 접종하여 $25^{\circ}C$ 암소에서 100 rpm으로 진탕배양하였을 때 세포생장은 배양 후 15일에 절정에 달하였으나 단위세포당 POD 활성(unit/g dry cell wt)은 배양 25일에 약 6,800으로 이는 실생 서양겨자무뿌리의 것보다 약 30배 높았다. 배양시기별 단위세포당 단백질 함량은 계대배양후 약간 높았다가 감소한 후, 배양 25일까지는 거의 일정한 값을 유지하다가 계속 배양함에 따라 감소하였으나 POD 비활성(unit/mg protein)은 배양후 12일부터 배양말기 (40일)까지 계속하여 증가하였다. 배양시기별 POD 동위효소의 패턴은 배양시기에 관계없이 거의 일정하였으나 배양 25일이후 산성의 주요 동위효소들의 활성이 약간 증가하였다. 이러한 결과로부터 고구마 세포배양의 POD는 세포생장 및 계대배양과 배지고갈로 인한 배양스트레스와 밀접한 관련 이 있을 것으로 간주된다. 본 연구에서 확립한 SP-47세포주는 하나의 동위효소가 강하게 발현되어 높은 활성을 보임으로써 새로운 POD 대량생산 시스템에 활용될 수 있을 것으로 기대된다.

• 한국식생활문화학회지
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• 제18권5호
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• pp.437-442
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• 2003

This study was conducted to confirm the effect of drying method on quality of popped rice for making salyeotgangjung(popped rice lump with malt syrup) in process of drying gelatinized rice. The drying were performed at dry oven or convection oven, at $30^{\circ}C,\;50^{\circ}C\;and\;105^{\circ}C$, respectively. There were not significant differences in the hardness of popped rice among the drying methods. The dry at $30^{\circ}C$ constant in dry oven showed higher popping rate, good external surface, uniformed and well developed internal cell size. The dry at $30^{\circ}C$ in convection oven and $50^{\circ}C$ constant temperature in dry oven showed low expansion and poor external surface, respectively. In conclusion, the drying at $30^{\circ}C$ in non-convection oven had the highest quality of popped rice for salyeotgangjung.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.192-197
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• 2005

The effects of macronutrients $(NO_3^-,\; NH_4^+\;and\;PO_4^{3-})$ on cell growth and triterpenoids production in Centella asiatica cell suspension cultures were analyzed using the Box­Behnken response surface model experimental design. In screening and optimization experiments, $PO_4^{3-}$ as a single factor significantly influenced cell growth where increasing the phosphate level from 0.1 to 2.4 or 2.6 mM, elevated cell growth from 3.9 to $14\~16g/L$. The optimum values predicted from the response surface model are 5.05mM $NH_4^+$, 15.0mM $NO_3^-$ and 2.6mM $PO_4^{3-}$, yielding 16.0g/L cell dry weight with $99\%$ fitness to the experimental data. While the $NH_4^+-NO_3^-$ interaction influenced cell growth positively in the optimization experiment, $NH_4^+$ and $NO_3^-$ as single factors; and interactions of $NO_3^--PO_4^{3-},\;NH_4^+-PO_4^{3-}$ and $NH_4^+-NO_3^-$ were all negative in the screening experiment. Cell growth and the final pH level were positively affected by $PO_4^{3-}$, but negatively affected by $NH_4^+\;and\;NH_4^+-PO_4^{3-}$ interactions. The different effects of factors and their interactions on cell growth and final pH are influenced by a broad or narrow range of macronutrient concentrations. The productions of triterpenoids however were lower than 4mg/g cell dry weight.

• 한국미생물·생명공학회지
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• 제19권2호
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• pp.179-185
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• 1991

We examined optimum culture conditions of Rhodopseudomonas sphaeroides B5 for effective utilization of substrate and sunlight for hydrogen production. The optimum concentration range of DL-lactate as electron donor for hydrogen production by resting cells was from 5 to 50mM, and optimun CN ratio (lactate/glutamat) for maintenence of hydrogen production activity by growing cultures was from 5 to 6. Hydrogen production by the cultures of low cell density (0.36mg/ml dry cells) was saturated with 10 Klux light intensity. Under constant illumination of 50Klux which was set up as the average medium value of annual variation of sunlight intensity, hydrogen production with various cell densities in the culture resulted in highest production rate (132${\mu}$l/hr/mg dry cells) up to 0.64mg/ml dry cells. However, the amount of total hydrogen production was saturated with cell density of 2.1mg/ml dry cells. In addition to these, the optimum inner thickness pervious to light of the culture vessel for hydrogen production which was measured under sunlight was 5 cm.

• 운동영양학회지
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• 제23권4호
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• pp.14-22
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• 2019

[Purpose] Here, we aimed to determine the effect of Polygonum cuspidatum stem extract (PSE) on exorbital lacrimal gland-excised rat models and hyperosmotic stress-stimulated human conjunctival cells (HCCs). [Methods] Seven week old male Wistar rats were divided into six groups. Only the rats in the control group (NOR, n=5) did not undergo surgery. Three days after the surgery, the exorbital lacrimal gland-excised rats were randomly allocated to five groups: (1) vehicle-treated dry-eyed rats (DED, n=5); (2) PSE (10 mg/kg) treated DED rats (PSE-10, n=5); (3) PSE (100 mg/kg) treated DED rats (PSE-100, n=5); and (4) PSE (250 mg/kg) treated DED rats (PSE-250, n=5). In addition, the HCC line was co-treated with hyperosmolar media (528 mOsm) and PSE (1-100 μg/ml). [Results] PSE treatment restored the tear volume and goblet cell density by inhibiting severe corneal irregularities and damage. The treatment with PSE significantly attenuated the hyperosmolar stress-induced inflammation and cell death through the suppression of mRNA expression levels of Tumor necrosis factor-α (TNF-α), Interleukin-6 (IL-6), Interleukin-1β (IL-1β), and Interferon-γ (IFN-γ), and the expression of Bcl-2-associated X protein (Bax) as well as the activation of caspase-3 in vitro. [Conclusion] The inhibitory effects of PSE treatment on dry eye disease indicate the potential of nutritional intervention by PES against inflammatory diseases without adverse effects.