• Title/Summary/Keyword: Drug education

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Preparation of 5-fluorouracil-loaded Nanoparticles and Study of Interaction with Gastric Cancer Cells

  • Fan, Yu-Ling;Fan, Bing-Yu;Li, Qiang;Di, Hai-Xiao;Meng, Xiang-Yu;Ling, Na
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.18
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    • pp.7611-7615
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    • 2014
  • Aims: To prepare 5-fluorouracil (5-Fu) nanoparticles with higher encapsulation efficiency and drug loading, and then investigate interaction with the SGC-7901 gastric cancer cell line. Materials and Methods: Prescription was optimized by orthogonal experiments, the encapsulation efficiency and loading capacity were tested by high-performance liquid chromatography, and inhibition of proliferation by 5-Fu nanoparticles and 5-Fu given to cells for 24, 48 and 72 hours was investigated by methyl thiazolyl tetrazolium assay (MTT). In addition, 5-Fu nanoparticles were labeled by fluorescein isothiocyanate (FITC), and absorption into cells was tested by flow cytometry. Results: The optimal conditions for preparation were concentrations of 5-Fu of 5mg/ml, of $CaCl_2$ of 60 mg/ml and of chitosan of 2 mg/ml. With a stirring speed of 1200rpm, encapsulation efficiency of 5-Fu nanoparticles was $55.4{\pm}1.10%$ and loading capacity was $4.22{\pm}0.14%$; gastric cancer cells were significantly inhibited by 5-Fu nanoparticles in a time and concentration dependent manner, and compared to 5-Fu with slower drug release, in a certain concentration range, inhibition with 5-Fu nanoparticles was stronger. 5-Fu nanoparticles were absorbed by the cells in line with the concentration. Conclusions: 5-Fu nanoparticles can inhibit growth of gastric cancer cells in vitro to a greater extent than with 5-Fu with good adsorption characteristics, supporting feasibility as a carrier.

An Epidemiological Study for on Outbreak of Typhoid Fever in Busan Area (부산 일부지역에 유행한 장티푸스 환자에 대한 역학적인 조사)

  • Park, Chin-Hyung
    • Journal of Preventive Medicine and Public Health
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    • v.10 no.1
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    • pp.86-93
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    • 1977
  • An epidemiological study was conducted, from April 28 to June 3, 1975, to investigate source and course of infection of typhoid fever occurred in Munhyeon Dong, Nam Gu, Busan. The obtained results were as follows: 1. The entity of unknown febrile disease was identified as Salmonella typhi, D group and it's symptoms were abdominal pain, diarrhea, hepatomegaly, nausea and vomiting, aplenomegaly, rose spots, tenderness of ileocecal region etc., in that order. 2. The average duration from onset to recovery was 25.2 days and incidence rate was 39.5 per 1,000 population. 3. The source of infection was presumed a charity patient. 4. By the duration of report on the cases from onset, 10-19 days group was the highest. 5. The positive rate of initial stool culture was 38.9% and secondary was 5.6%. 6. Distribution of the cases by age and sex showed that 5-9 years old group was the highest as 30.5%, and male was higher than female. 7. Distribution of the cases by education level, under primary school as 66.6% was the highest. By the living standard, the highest was low income earner group as 77.8%. 8. Utilization rate of medical facilites was drug store (41.7%), herbal drug (8.3%), clinic (5.6%) in that order.

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Serum total Cholesterol Levels and Related Factors in a Rural Adult Population (농촌지역 성인들의 혈청 총콜레스테롤치 및 관련요인)

  • Ahn, Soon-Ki;Kam, Sin;Jin, Dae-Gu;Kim, Jong-Yeon;Chun, Byung-Yeol
    • Journal of Preventive Medicine and Public Health
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    • v.35 no.3
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    • pp.255-262
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    • 2002
  • Objectives : To investigate the fetal cholesterol levels and related factors in a rural adult population. Methods : 3,207 adults(1,272 men and 1,935 women) were examined in 1997 Their heights, weights, and fasting serum total cholesterol levels were measured, and their body mass indices were calculated. A questionnaire interviewing method was used to collect risk factor data. Results : The mean value of total cholesterol were 190.5 mg/dl, and 198.8 mg/dl, and the age-adjusted prevalence rates for hypercholesterolemia (above 240 mg/dl) were 13.7% and 10.2%, in men and women, respectively. From simple analyses, age, educational level, coffee intake, amount of meat and food intake, waist circumference, waist-hip circumference ratio(WHR), and body mass index(BMI) were significant risk factors in men (p<0.05) relating to serum total cholesterol levels. In women, age, educational level, hypertension history, diabetes history, herbal drug history, amount of feed intake, alcohol consumption, waist circumference, WHR, BMI, and menopausal status were significant risk factors (p<0.05). In multiple linear regression analyses, waist circumference (p<0.01), BMI (p<0.01), and coffee intake (p<0.05) proved to be significant risk factors in men. Whereas, menopausal status (p<0.01), BMI (p<0.01), herbal drug history (p<0.01), amount of food intake (p<0.05), waist circumference (p<0.05), and alcohol consumption (p<0.05) were significant risk factors in women. Conclusions : The significant risk factors, for both men and women, relating to the serum total cholesterol were waist circumference and BMI. Thus, for the reduction of serum total cholesterol level, it is recommended that health education for the control of obesity should be implemented.

Factors Affecting Performance Infection Control of MultiDrug-Resistant Organisms among Nurses:with focus of the Health Belief Model (종합병원 간호사의 다제내성균 감염관리 수행도에 영향을 미치는 요인: 건강신념모델 중심으로)

  • Park, Jung Hee;Lee, Mi-Hyang
    • Journal of Digital Convergence
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    • v.18 no.3
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    • pp.227-234
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    • 2020
  • This study was carried out to provide basic data for the development of an educational program by assessing the its effects on the MDROs infection management performances of nurses at general hospitals. Data was obtained through a structured questionnaire survey conducted on 202 nurses. The average of knowledge score was 17.68 and their perceived susceptibility, perceived severity, perceived benefits, and performances had correlation. There was difference in their performances in accordance with their general characteristics including marriage, clinical experiences, positions, and affiliated department. Factors that affected MDROs infection management performance included perceived benefits and affiliated department with an explanation power of 35%. Therefore, it is necessary to emphasize the benefits by including advantages and effects of performing the MDROs infection control at the time of the development. In addition, nurses are required to have MDROs infection control education.

Effects of Weight Reduction Program on Body Weight, Self Esteem and Self Efficacy of Chronic Mentally Ill Persons (체중관리 프로그램이 만성정신질환자의 체중, 자아존중감 및 자기효능감에 미치는 효과)

  • Kim, Su Yeon;Kim, Soo Jin
    • Journal of Korean Public Health Nursing
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    • v.29 no.3
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    • pp.594-607
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    • 2015
  • Purpose: The purpose of this study was to test the effectiveness of a weight reduction program on the body weight, BMI, self-esteem, and self-efficacy of chronic mentally ill persons. Methods: A nonequivalent control group pre-posttest design was employed. Participants were 46 chronic mentally ill persons who agreed to participate in this study, and were assigned to an experimental group (n=23) and a control group (n=23). The weight reduction program was modified by the author, based on the Wellness program (Lilly Pharmaceutical Company, 2003), Lee (2007) and Kim (2013). Data analysis with frequency, ${\chi}^2$-test, Fisher's exact test, and t-test was performed using SPSS/Win 18.0 version. Results: The results showed a significant decrease in body weight and BMI and an increase in self-esteem in the experimental group compared to the control group. Conclusion: The weight reduction program had a positive effect on body weight, BMI, and self-esteem. Evaluation of drug compliance and vocational rehabilitation is recommended for long term effect of the program and conduct of further studies will be required for use of various resources for self-efficacy for inclusion in drug education in a program for improved health outcome.

The Association between Adolescents Risk Behavior and Smoking (청소년 위험행동과 흡연과의 상관성)

  • Lee, Jun Beom;Hahm, Myung-Il;Kim, Dong Jun;Cho, Ha-Hyeon;Min, In Soon
    • Korea Journal of Hospital Management
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    • v.24 no.2
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    • pp.12-22
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    • 2019
  • Purposes: The purpose of this study was to examine the association between risk behaviors and smoking in Korean adolescents. Methods: This study used data from online survey of youth health behavior in 2017. Data from a total of 54,411 people (27,139 male, 27,272 female) were included in the analysis. chi-square test, simple logistic regression, multiple logistic regression were performed using SAS 9.4. Findings: Multiple logistic regression analysis showed that risk behaviors such as drinking alcohol experience, sexual experience, drug use experience and high caffeine energy drinks intake experience had a significant effect on smoking. Adolescents with drinking experience were more likely to smoking than those who had no experience(OR=8.58, 95% CI: 7.67~9.60). Adolescents with sexual experience were more likely to smoking than those who had no experience(OR=4.47, 95% CI: 3.91~5.11). Adolescents with drug use experience were more likely to smoking than those who had no experience(OR=2.32, 95% CI: 1.63~3.32). Also, adolescents with high-caffeine energy drinks intake experience were more likely to smoking than those who had no experience(OR=1.37, 95% CI: 1.23~1.53). Practical Implications: All the risk behaviors were significantly associated with smoking rates. Results of this study suggest that physicians and health workers in medical institutions and health centers should simultaneously serve education and consultation for the smoking cessation as well as for the prevention of risk behaviors.

Ustekinumab pharmacokinetics after subcutaneous administration in swine model

  • Grabowski, Tomasz;Burmanczuk, Artur;Derlacz, Rafal;Stefaniak, Tadeusz;Rzasa, Anna;Borkowski, Jacek
    • Journal of Veterinary Science
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    • v.22 no.5
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    • pp.47.1-47.10
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    • 2021
  • Background: Due to multiple similarities in the structure and physiology of human and pig skin, the pig model is extremely useful for biological drug testing after subcutaneous administration. Knowledge of the differences between subcutaneous injection sites could have a significant impact on the absorption phase and pharmacokinetic profiles of biological drugs. Objectives: This study aimed to analyze the impact of administration site on pharmacokinetics and selected biochemical and hematological parameters after a single subcutaneous administration of ustekinumab in pigs. Drug concentrations in blood plasma were analyzed by enzyme-linked immunosorbent assay. Pharmacokinetic analyses were performed based on raw data using Phoenix WinNonlin 8.1 software and ThothPro v 4.1. Methods: The study included 12 healthy, female, large white piglets. Each group received a single dose of ustekinumab given as a 1 mg/kg subcutaneous injection into the internal part of the inguinal fold or the external part of the inguinal fold. Results: The differences in absorption rate between the internal and external parts of the inguinal fold were not significant. However, the time of maximal concentration, clearance, area under the curve calculated between zero and mean residence time and mean residence time between groups were substantially different (p > 0.05). The relative bioavailability after administration of ustekinumab into the external part of the inguinal fold was 40.36% lower than after administration of ustekinumab into the internal part of the inguinal fold. Conclusions: Healthy breeding pigs are a relevant model to study the pharmacokinetic profile of subcutaneously administered ustekinumab.

A Study on the Drug Prevention Education of VR Content Model (마약 예방 교육을 위한 가상현실(VR) 콘텐츠 모델 설계)

  • Cho Jae-cheon;Jeon Seong-hwan;Moon Tae-woong;Lee Jae-ho;Jang Sangmin;Kim Junbae;Lee Sang-min;Kim Seungwon;Kang Sangyoon;Yoo Jeong-hyun;Sun-Geo Hong;Seok-Hun Kim
    • Proceedings of the Korean Society of Computer Information Conference
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    • 2023.07a
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    • pp.401-402
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    • 2023
  • 2023년 기준 대한민국은 전체 마약 사범 가운데 30대 이하의 마약 사범이 전체 마약 사범의 59.7%를 차지하고 있고, 인터넷에 익숙한 청소년이 '다크 웹'이나 SNS를 통한 밀매는 일상이 되고 있는 상황이다. 본 논문에서는 VR로 마약의 부작용을 간접체험할 수 있도록 상대적으로 VR에 친숙한 젊은 층 연령에 맞춰 실제 마약 범죄 사례를 재현하고 이를 통해 가상 현실 내 여러 가지 콘텐츠로 다각도 지식 함향을 할 수 있는 VR 교육 콘텐츠를 제안하고자 한다.

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Understanding centrosome amplification in cancer: A pathway toward precision-targeted cancer drug development (암의 중심체 증폭 이해를 통한 표적 항암제 개발)

  • Taekyung Kim
    • Journal of Life Science
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    • v.33 no.11
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    • pp.950-955
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    • 2023
  • Cell division is an essential process for the survival and development of living organisms. It is critical that duplicated chromosomes are properly segregated into daughter cells during mitosis. The centrosome is the core organelle that forms the microtubule-organizing center (MTOC), which generates the microtubules that make up the mitotic spindle during cell division. The centrosome is also involved in cell signaling and motility. In normal cells, there is one centrosome in G1 that replicates into two in the S phase and matures through G2. During the M phase, duplicated centrosomes move to both ends of the cell, and spindle microtubules that are generated from MTOC move the chromosome to both ends. The cells then split into two to complete the cell division. However, a phenomenon called centrosome amplification (CA), in which the number of centrosomes is higher than normal, is common in cancer cells and can lead to chromosome instability (CIN). This paper discusses the process of centrosome replication and the role of PLK4 in this process. The possible consequences of centrosome amplification and how the PLK4 inhibitor may be able to treat certain types of cancer cells, such as breast cancer and neuroblastoma, will also be discussed.

Antibiotic Reversal Activity of Piper longum Fruit Extracts against Staphylococcus aureus Multi-Drug Resistant Phenotype

  • Maryam Salah Ud Din;Umar Farooq Gohar;Hamid Mukhtar;Ibrar Khan;John Morris;Soisuda Pornpukdeewattana;Salvatore Massa
    • Microbiology and Biotechnology Letters
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    • v.51 no.4
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    • pp.432-440
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    • 2023
  • Irrational and injudicious use of antibiotics, easy availability of them as over-the-counter drugs in economically developing countries, and unavailability of regulatory policies governing antimicrobial use in agriculture, animals, and humans, has led to the development of multi-drug resistance (MDR) bacteria. The use of medicinal plants can be considered as an alternative, with a consequent impact on microbial resistance. We tested extracts of Piper longum fruits as new natural products as agents for reversing the resistance to antibiotics. Six crude extracts of P. longum fruits were utilized against a clinical isolate of multidrug-resistant Staphylococcus aureus.The antibiotic susceptibility testing disc method was used in the antibiotic resistance reversal analysis. Apart from cefoxitin and erythromycin, all other antibiotics used (lincosamides [clindamycin], quinolones [levofloxacin and ciprofloxacin], and aminoglycosides [amikacin and gentamicin]) were enhanced by P. longum extracts. The extracts that showed the greatest synergy with the antibiotics were EAPL (ethyl acetate [extract of] P. longum), n-BPL (n-butanol [extract of] P. longum), and MPL (methanolic [extract of] P. longum The results of this study suggest that P. longum extracts have the ability to increase the effectiveness of different classes of antibiotics and reverse their resistance. However, future studies are needed to elucidate the molecular mechanisms behind the synergy between antibiotic and phytocompound(s) and identify the active biomolecules of P. longum responsible for the synergy in S. aureus.