• Proceedings of the Korean Society of Sericultural Science Conference
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• 2003.10a
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• pp.147-148
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• 2003

The purpose of this experiment is to confirm whether the recombinant tumstatin revealed from the stably transformed Drosophila melanogaster S2 cells has in vitro capacity. Materials and Methods : Materials - Cell line : Drosophila melanogaster Schneider 2 (S2) cells - vector pMT/BiP/V5-His and pCoHygro (Invitrogen) (omitted)

• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.563-568
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• 2002

Stably transformed Drosophila melanogaster 52 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in 52 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.

• The Korean Journal of Zoology
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• v.9 no.2
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• pp.21-24
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• 1966

1. The differentiation process of the cluster cells of compound eye in wild typed Drosophila melanogaster, born in Heuk San Do, Korea, was observed. 2. The eye disc of Drosophila melanogaster is originated from the frontal sac before hatching. 3. Clusteris bgin to form at the posterior end of the eye disc at the 2 nd instar larval stage, and the completed to differentiate in the eye disc at the 96 hours after hatching . 4. At the pupa stage, the eye cups are formed by clusters. 5. The eye cup begins to elongate and is changed into an ommatidium. 6. All organs of an ommatidium are completed in an omatidium at the old pupa stage(156-160 hours after hatching. )

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.6-11
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• 1990

Analysis of genetic structure in Kimpo natural population of Drosophila was carried out by utilizing the deleterious gene on the second chromosome of Drosophila melanogaster. Male flies tested were continuously collected for eight years; in late September 1974 and 1981-1987. The frequency of deleterious gene (lethal plus semilethal) ranged from 27.02% in 1983 to 41.48% in 1987, and the values estimated from the eight years samples are highly signihcent from each other with a homogenety test (X$^2$=52.0157, d.f.=28, P<0.005). Allelic rates ranged from 1.30% in 1981 to 5.03% in 1974. And the effective population size by using the rate of allelism was estimated average at 3, 300 pairs. Elimination rate by homozygous of lethal gene ranged from 0.0004 in 1984 to 0.0019 in 1974, and that is for smaller than mutation rate(0.005) at second chromosome. We suppose that stable frequency (about 20%) lethal genes of D. melanogaster in Kimpo natual population are maintained by invade of P-type mutator factor (P element) versus eliminated in heterozygous and homozygous condition of lethal gene.

• Journal of Life Science
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• v.13 no.3
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• pp.252-257
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• 2003

The genetic variabilities of second chromosomes of Drosophila melanogaster concealed in Busan natural and experimental populations have been analyzed by the Cy//Pm method. The experimental population was composed of D. melanogaster which had the lethal-free second chromosome, collected Sasang natural population in 1982. The frequencies of deleterious genes were estimated to be 14.3∼25.4% in Busan natural population and 26.5∼27.2% in experimental population. The allelism rates in lethal genes isolated from the natural and experimental populations were calculated to be about 0.76% and 9.76∼14.17%, respectively. The value of elimination by the frequencies of deleterious genes and allelism rate was 0.0106and the effective population size estimated to be about 430 flies at the 6570 days population.

• Journal of Life Science
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• v.22 no.5
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• pp.621-626
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• 2012

$Drosophila$ $melanogaster$ has been used as a useful model to study development and disease. In this study, we established the primary culture method of $Drosophila$ in the intestine to understand how intestinal stem cells (ISCs) mediate tissue repair during infection and disease. To obtain intestinal cells, we separated intestines from adult flies and isolated single cells by enzymatic treatment. The survival of cultured cells was measured using MTS-analysis. The maximum growth rate of the cells was observed on the 9th day after seeding. In addition, the presence of ISCs and enteroendocrine cells was confirmed by delta and prospero staining. Accordingly, we supposed that $Drosophila$ $melanogaster$ gut cells established in this study are probably useful in studies about intestinal stem cell regulation and various diseases occurring in the intestine.

• Applied Biological Chemistry
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• v.44 no.4
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• pp.211-214
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• 2001

Utilizing the foreign gene expression system of Drosophila melanogaster Schneider line 2(S2) cell, the degree of transient protein and mRNA expression was examined with different terminators. In the case of transient expression, the expression level of green fluorescent protein(GFP) was the highest when the transfection agent was eliminated and then cultivated for 36 to 48 hr. The terminators(SV40 p(A), SV40 small T-antigen and human gastrin 3'UTR) of the expression vector system were each cloned with endostatin; thereafter, the expression levels of the endostatin and its mRNA were compared. When the expression levels of endostatin were compared 36 hr after transfection, the SV40 p(A) terminator showed the highest expression level of endostatin and its mRNA.

• The Korean Journal of Zoology
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• v.30 no.1
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• pp.10-28
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• 1987

Changes of protein synthesis in the isolated egg chambers were studied during oogenesis of Drosophila melanogaster. Protein synthesis did not change much in quantity except that the activity was slightly enhanced at the stages 11 and 12, when new structures such as vitelline membranes and yolk granules were made, but considerably changed in quality during oogenesis. Protein synthesis was believed to occur mainly in the follicle cells, and a number of stage-specific proteins were found to differ at various stages. The observations suggest that proteins synthesized in the cells within chambers as well as those from the outside of the chambers might be directly required for the growth and maturation of oocytes in Drosophila.

• Journal of Environmental Science International
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• v.4 no.5
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• pp.157-157
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• 1995

In order to analyze the sex-linked lethal mutagenic effects of ethyl methanesulfonate (EMS) in Drosophila melanogaster, the mutagenicities from the attached-X and Basc method have been detected. The indexes of relative lethal mutations at 2.0 and 4.0mM EMS treated group using the attached-X method were about 1.5 and 2.4 times than that of 1.0mM treated group, respectively. EMS had more pronounced effect in the sperm and spermatid stages in the induction of X-linked lethal mutations during the spermatogenesis. And, in the induction of X-linked recessive lethal mutations from the Basc method, the mutation frequency of 4.0mM EMS treated group as compared with 2.0mM was more than three fold. Two assay systems used in this study can support mutually according to experimental purposes, and the area of its application can be considerably wide.

• Journal of Environmental Science International
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• v.4 no.5
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• pp.543-549
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• 1995

In order to analyze the sex-linked lethal mutagenic effects of ethyl methanesulfonate (EMS) in Drosophila melanogaster, the mutagenicities from the attached-X and Basc method have been detected. The indexes of relative lethal mutations at 2.0 and 4.0mM EMS treated group using the attached-X method were about 1.5 and 2.4 times than that of 1.0mM treated group, respectively. EMS had more pronounced effect in the sperm and spermatid stages in the induction of X-linked lethal mutations during the spermatogenesis. And, in the induction of X-linked recessive lethal mutations from the Basc method, the mutation frequency of 4.0mM EMS treated group as compared with 2.0mM was more than three fold. Two assay systems used in this study can support mutually according to experimental purposes, and the area of its application can be considerably wide.