• Title/Summary/Keyword: Dried Layer

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DEVELOPMENT OF BIOCOMPATIBLE DRESSING MATERIAL MADE OF COLLAGEN AND AMNIOTIC MEMBRANE AND WOUND HEALING EXPERIMENT IN RAT (양막과 콜라겐을 이용한 생체 적합 드레싱 소재 개발 및 백서 창상치유 실험)

  • Ahn, Kang-Min;Lee, Ji-Ho;Lee, Ui-Lyong;Lee, Jong-Ho;Lee, Jong-Won;Kim, Sung-Po;Yang, Eun-Kyung;Kim, Ki-Ho
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.32 no.3
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    • pp.189-199
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    • 2006
  • Purpose of study: Partial thickness skin graft is the golden standard regimen for full-thickness skin defect caused by burn or trauma. However, in case of extensive burns of more than 50% of total body surface area, the donor site is not sufficient to cover all defects. As a second choice, allograft, xenograft and synthetic materials have been used to treat skin defect. Among them the amniotic membrane(AM) was used as a biological dressing for centuries because of its potential for wound healing. In this study, quantification of EGF in AM and effect of AM-collagen complex on full thickness skin defects was examined. Materials & Methods: The concentration of EGF in fresh, deep frozen and freeze-dried AM was evaluated by ELISA. EGF-R immunostaining was performed in freeze-dried AM. SD rats weighing 250${\sim}$300g was used for wound healing experiment. Three full thickness skin defects(28mm diameter) were made on dorsal surface of SD rat. The control group was covered by Vaselin gauze and AM-collagen complex and $Terudermis^{(R)}$. was grafted in two other defects. Healing area, Cinamon's score were evaluated before biopsy. Grafted sites were retrieved at 3 days, 1 week, 2 weeks and 4 weeks after operation. H & E and Factor VIII immunohistochemical stain was performed to evaluate the microscopic adhesion and structural integrity and microvessel formation. Results: 1. EGF concentration of fresh, deep frozen and freeze-dried AM showed similar level and EGF-R was stained in epithelial layer of freeze-dried AM. 2. At 4 weeks after grafting, the healing area of AM-collagen and Terudermis group was 99.29${\pm}$0.71% and 99.19${\pm}$0.77 of original size. However, that of control group was 24.88${\pm}$2.90. 3. The Cinamon's score of AM-Collagen and $Terudermis^{(R)}$. group at 4 weeks was 15.6${\pm}$1.26 and 14.6${\pm}$3.13 and that of control group was 3.7${\pm}$0.95. Significant difference was observed among control and experimental groups(p<0.05). 4. Histologic examination revealed that AM protected leukocyte infiltration and epithelial migration was nearly completed at 4 weeks. $Terudermis^{(R)}$. group showed mild neutrophil infiltration until 2 weeks and completion of epithelization at 4 weeks. Control group showed massive leukocyte infiltration until 4 weeks. 5. Microvessels were increased sharply at 1 week and control group at 1 and 4 week showed significant differences with $Terudermis^{(R)}$. group of same interval(p<0.05) but no differences were found with AM group(p<0.05). Conclusion: EGF and EGF-R were well preserved in freeze-dried AM. AM attached to collagen acted as excellent biologic dressing which had similar effect with $Terudermis^{(R)}$. AM showed anti-inflammatory action and healing was completed at 4 weeks after full-thickness skin defect.

Effects of Cynanchum spp. on the hyperlipidemia in rats induced by Triton WR-1339 (국산 및 중국산 백수오(白首烏)의 Triton WR-1339 유발 고지혈증(高脂血症) 흰쥐에 대한 효능 연구)

  • Choi, Ho-Young;Ham, In-Hye;Lee, Ju-Yeong;Yoon, Ye-Jin;Yang, Gab-Sik;Tae, Jin;Bu, Young-Min;Kim, Ho-Cheol
    • The Korea Journal of Herbology
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    • v.22 no.4
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    • pp.279-286
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    • 2007
  • Objective : In order to compare of hyperlipidemia effect of C. wilfordii, C. auriculatum in korea and C. auriculatum in China, it was performed on the hyperlipidemia in rats induced by Triton WR-1339. Method : The 70% MeOH extracts from dried root of C. wilfordii and C. auriculatum in korea and C. auriculatum in china were fractionated into H2O layer and CHCl3 layer. The level of total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol, AST and ALT in serum were used to observe the effectiveness of C. wilfordii korean C. auriculatum and chinese C. auriculatum. Results : In the hyperlipidemic rats induced by Triton WR-1339, C. wilfordii and korean C. auriculatum significantly decreased total cholesterol, triglyceride, otherwise significantly increased HDL. And C. wilfordii and chinese C. auriculatum significantly decreased AST and ALT. Conclusion : This study indicate that C. wilfordii korean C. auriculatum and chinese C. auriculatum were effective in the hyperlipidemic rats induced by Triton WR-1339. According to the results, C. wilfordii korean C. auriculatum and chinese C. auriculatum need to considered for the application in hyperlipidemia.

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Analysis of Factors Affecting the Slope Stability of Uncontrolled Waste Landfill (비위생 폐기물 매립지 사면의 안정성에 관한 영향인자 분석)

  • Yoo, Han-Kyu;Choi, Bong-Hyuck
    • Journal of the Korean GEO-environmental Society
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    • v.3 no.1
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    • pp.5-12
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    • 2002
  • The effects of ground water level, shear strength parameters of refuse, and geological condition of ground on the slope stability of uncontrolled waste landfill were studied. The Janbu method of slices based on the limit equilibrium method was used to calculate the minimum factor of safety with respect to slope stability of landfill. The analytical results showed that the factor of safety for a fully dried condition of landfill increased 2.4~2.8 times as great as that for a fully saturated condition of landfill. Under the condition of actual ground water level, the factor of safety linearly increased with increasing both cohesion and internal friction angle of refuse. Also, when the potential failure surface passed through the underlying layer, the factor of safety and shape of potential failure surface were found to depend on geological conditions of underlying layer.

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Isolation and Identification of Antimicrobial Compound from Mokdan Bark (Paeonia suffruticosa ANDR) (목단피로부터 식품부패 미생물에 대한 항균성 물질의 분리 및 동정)

  • 황재선;한영실
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.7
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    • pp.1059-1065
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    • 2003
  • Antimicrobial activity of Mokdan bark (Paeonia suffruticosa $A_{NDR}$) was investigated. Methanol extract of dried Mokdan was fractionated to hexane, chloroform, ethylacetate, butanol and aqueous fraction. Ethylacetate fraction among these fractions showed the highest inhibitory effect on the microorganisms such as L. monocytogenes, and E. coli at 500 $\mu\textrm{g}$/disc. Ethylacetate fraction was further fractionated into 3 fractions by silica gel column and thin layer chromatography (TLC). The results showed that ethylacetate fractions No. 1 and 2 had the highest antimicrobial activity. They were mixed again, reseparated, and 3 fractions were obtained. Among them, No. 1 had the highest inhibitory effect on the microorganisms, No. 1 fraction was identified as isobutyl isopentanoate by HPLC, and GC-MS.

The Characterization of Metal Silicon and Compacts for the Nitridation (질화반응용 금속규소 및 그 Compacts의 Characterization(Densification of Silocon Nitride 1보))

  • 박금철;최상욱
    • Journal of the Korean Ceramic Society
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    • v.20 no.3
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    • pp.211-216
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    • 1983
  • This work aims at characterizing silicon grains and its compacts. In order to remove iron silicon grains were washed with 5N hydrochloride at 60-7$0^{\circ}C$ for 170 hrs, and then followed the chemical analysis by atomic absorption spectrophotometer X-ray diffraction analysis SEM observation and specific surface area determination by B. E. T. Mixtures of graded silicon particles with two or three different sizes were made into packings by mechanical vibration. The mixtures were used to make compacts with 10 mm in diameter and 70mm in length by isostatically pressing at 1, 208 kg/$cm^2$ (20 kpsi) and 4, 255kg/$cm^2$ (60 kpsi) respectively. Bulk densities of packings and compacts were measured. A slip made of magnesium nitrate solution and fine silicon particles was spray-dried and then decomposed at 30$0^{\circ}C$ for the purpose of coating the uniform layer of magnesium oxide on the surface of particles. The results obtained are as follows: (1) About two thirds of iron content could be removed from silicon by washing silicon powders with hydrochloride. (2) Uniform layer of magnesium oxide on the surface of silicon could be prepared by spray-drying suspension and by decomposing it. (3) B. E. T. specific surface area of fine silicon particles was 2, 826.753$m^3$/kg. (4) In the binary system with two sizes of 40-53$\mu\textrm{m}$ particles and <10$\mu\textrm{m}$ particles the maximum bulk density of packing was 55% of theoretical value and that of compacts made at the pressure of 4, 255 kg./$cm^2$ (60 kpsi) was 73% of theoretical value. (5) In the ternary system with three sizes the maximum bulk density of packing was 1.43 g/$cm^3$and that of compacts was 1.80g/$cm^3$which is equivalent to 77.6% of theoretical value. The composition of the closest compact was consisted of 50% of 40-53$\mu\textrm{m}$ particles 20% of 10-30$\mu\textrm{m}$ particles and 30% of <10$\mu\textrm{m}$ parti-cles.

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Evaluation of Durability for Al Alloy with Anodizing Condition (알루미늄 합금의 양극산화 조건에 따른 내구성 평가)

  • Lee, Seung-Jun;Han, Min-Su;Kim, Seong-Jong
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2016.11a
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    • pp.152-152
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    • 2016
  • Anodizing is a technology to generate thicker and high-quality films than natural oxide films by treating metals via electrochemical methods. It is a technique to develop metals for various uses, and extensive research on the commercial use has been performed for a long time. Aluminum anodic oxide (AAO) is generate oxide films, whose sizes and characteristics depending on the types of electrolytes, voltages, temperatures and time. Electrochemical manufacturing method of nano structure is an efficient technology in terms of cost reduction, high productivity and complicated shapes, which receives the spotlight in diverse areas. The sulfuric acid was used as an anodizing electrolyte, controlling its temperature to $10^{\circ}C$. The anode was 5083 Al alloy with dimension of $5(t){\times}20{\times}20mm$ while the cathode was the platinum. The distance between the anode and the cathode was maintained at 3 cm. Agitation was introduced by magnetic stirrer at 300 rpm to prevent localized temperature rise that hinders stable growth of oxide layer. In order to observe surface characteristics with applied current density, the electrolyte temperature, concentration was maintained at constant condition for $10^{\circ}C$, 10 vol.%, respectively. To prevent hindrance of stable growth of oxide layer due to local temperature increase during the experiment, stirring was maintained at constant rate. In addition, using galvanostatic method, it was maintained at current density of $10{\sim}30mA/cm^2$ for 40 minutes. The cavitation experiment was carried out with an ultrasonic vibratory apparatus using piezo-electric effect with modified ASTM-G32. The peak-to-peak amplitude was $30{\mu}m$ and the distance between the horn tip and specimen was 1 mm. The specimen after the experiment was cleaned in an ultrasonic, dried in a vacuum oven for more than 24 hours, and weighed with an electric balance. The surface damage morphology was observed with 3D analysis microscope. As a result of the investigation, differences were observed surface hardness and anti-cavitation characteristics depending on the development of oxide film with applied current density.

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Sectional Composition of Minerals in Domestic Deer Antler (국내산 녹용(Cervi parvum Cornu) 의 부위별 무기질 조성)

  • Kim, Hye-Young;Rhyu, Mee-Ra
    • Korean Journal of Food Science and Technology
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    • v.32 no.1
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    • pp.31-36
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    • 2000
  • The mineral compositions were analysed of the tip and the upper section of dried domestic antlers. The upper section was divided longitudinally into four parts. The tip and the upper section were divided traversely into the velvet and the spongy bone layers. The contents of ash, calcium, phosphorus and magnesium increased (p<0.05), and those of manganese, zinc, copper and iron decreased (p<0.05) downward from the tip both in the velvet and the spongy bone layers. The contents of ash, calcium, phosphorus, manganese and zinc of the spongy bone layer were significantly (p<0.05) higher than those of the velvet layer. The tip, which is the growth center of the antler, had the significantly (p<0.05) lowest proportions of Ca/ash, Ca/P and Ca/Fe ratios both in the velvet and spongy bone layers.

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Quality Characteristics of Yellow Layer Cake Added with Sparassis crispa Powder (꽃송이버섯 분말을 첨가한 옐로우 레이어 케이크의 품질특성)

  • Jang, Woo Hyuk;Yoo, Young-Bok;Kim, Byung Hee;Bae, Song-Hwan
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.42 no.12
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    • pp.1988-1993
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    • 2013
  • The aims of this study were to investigate the quality characteristics of yellow layer cake added with Sparassis crispa powder that were abundant in dietary fiber, and also to determine the most suitable amount of added S. crispa powder. Dried powder of S. crispa containing 61.8 g/100 g insoluble dietary fiber was added to the cake batter in Baker's percentage of 1%, 2%, 4%, and 8%. Cake batter containing 1% and 2% S. crispa powder showed similar pH, specific gravity, and viscosity values to the batter without S. crispa powder (control). As the amount of added S. crispa power increased, the volume, specific volume, baking loss, and brightness (for both crust and crumb) of the cake containing the powder tended to decrease. A greater amount of added S. crispa powder resulted in a decrease in hardness, gumminess, and chewiness of the cake containing the powder and also reduced the changes in hardness of the cake during 8 days of storage. The sensory analysis showed that cake containing 2% S. crispa had several desirable kinds of sensory attributes, such as color, flavor, taste, and texture compared to the control; whereas an addition of more than 2% S. crispa deteriorated the sensory quality of the cake. These results suggest that the most suitable amount of added S. crispa powder for preparing yellow layer cake was 2% in Baker's percentage.

Strength Properties of GFRP Reinforced Glulam Beams Bonded with Polyvinyl Acetate-Based Emulsion Adhesive (초산비닐수지계 접착제를 사용한 유리섬유강화플라스틱 복합집성재의 강도 성능 평가)

  • Park, Jun-Chul;Hong, Soon-Il
    • Journal of the Korean Wood Science and Technology
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    • v.36 no.4
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    • pp.19-25
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    • 2008
  • This study was carried out to investigate the bending strength properties of the unreinforced glulam beams and the GFRP laminated glulam beams according to the volume ratio of GFRP. The 7-layer glulam beams ($10cm(b){\times}14cm(h){\times}180cm(l)$) were manufactured, using Larch (Larix kaempferi Carr.) laminae ($2cm(h){\times}10cm(b){\times}360cm(l)$), which were dried to the moisture content of 8% and specific gravity of 0.54. GPRP of 0.1 and 0.3 cm was reinforced between the outmost layer of bottom and next layer. When the glulam beams were reinforced with GFRP at the volume ratio of 0.7% and 2.1%, respectively, the bending strength was increased by 12% and 28%, respectively, in the reinforced beams than in control glulam beams. Also, the GFRP reinforced layer of the glulam beams with GFRP laminations blocked the progression of rupture, and the unbroken part held about 90% of the bending strength. In the results of glue joints test, the block shear strength is higher than $7.1N/mm^2$, the standard of KS F3021, and in the result of delamination, the adhesive strength is good as the water soaking and boiling delamination was less than 5%.

Effect of Recycling on the Papermaking Properties of Wood Pulp Fibers (펄프섬유(纖維)의 제지특성(製紙特性)에 미치는 회수처리(回收處理)의 영향(影響))

  • Kim, Hyoung-Jin;Jo, Byoung-Muk
    • Journal of the Korean Wood Science and Technology
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    • v.21 no.1
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    • pp.21-38
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    • 1993
  • In order to investigate the influence of recycling, a laboratory method simulating the papermaking process was used for assessing the effects of recycling on fiber properties. Sw-BKP, Hw-BKP and BGP were disintegrated and beaten to about 42$^{\circ}$SR-44$^{\circ}$SR by a valley beater. After beating, these pulps were dewatered by centrifuge and dried at 90$^{\circ}C$ for 72hrs. This recycling process(sequence of wetting, defiberating, dewatering and drying) was repeated seven times. Physical, mechanical and optical properties of recycled pulps were evaluated by TAPPI Standards. Morphological changes occurred through recycling process was observed by SEM. Sheet density decreased with recycling. The largest drop in density occurred during the first recycling. The porosity values decreased with recycling. Mechanical properties such as tensile, burst strength and folding endurance, decreased with recycling. However tear strength of Sw-BKP and mixtured pulp increased at the first recycling. Optical properties such as brightness, opacity and light scattering coefficient, increased with recycling. However, brightness of mixtured pulp gradually decreased with recycling. Fibrillated outer layer of the fiber was gradually removed from the surface with recycling. As a result of recycling, crinkles on the fiber surface were found to be more folded.

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