• 한국토양비료학회지
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• 제49권3호
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• pp.251-258
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• 2016

A rhizobacterium LPN-2, which showed strong antifungal activity and auxin producing ability, was isolated from a farmland in North Gyeongsang Province, South Korea. Based on analysis of the 16S rDNA sequence, strain LPN-2 was identified as a novel strain of Burkholderia and was designated as Burkholderia sp. LPN-2. In vitro experiments showed that the isolated stain LPN-2 significantly produced auxin within 48 hr incubation. In order to check for PGPR function we performed in vivo growth promoting test in different crops, including mung bean, pea and cabbage. Application of Burkholderia sp. LPN-2 showed dramatic growth promoting effect on all the tested plants. We also confirmed siderophore and cellulase productions by Burkholderia sp. LPN-2 using CAS blue agar and CMC plate test. Further treatment with LPN-2 and the crude culture broth was effective in suppressing anthracnose in vitro test and also reduced incidence and severity of anthracnose in apple and pepper. Taken together, we conclude that Burkholderia sp. LPN-2 might be used as organic fertilizer for effective crop production in organic farming.

• 한국환경과학회지
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• 제17권12호
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• pp.1413-1419
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• 2008

The Kyoto Protocol, that had been in force from February 16, 2005, requires significant reduction in $CO_2$ emissions for all anthropogenic sources containing transportation, industrial, commercial, and residential fields, etc, and automotive emission standards for air pollutants such as particulate matter (PM) and nitrogen oxides $(NO_x)$ become more and more tight for improving ambient air quality. This paper has briefly reviewed homogeneous charge compression ignition (HCCI) combustion technology offering dramatic reduction in $CO_2,\;NO_x$ and PM emissions, compared to conventional gasoline and diesel engine vehicles, in an effort of automotive industries and their related academic activities to comply with future fuel economy legislation, e.g., $CO_2$ emission standards and corporate average fuel economy (CAFE) in the respective European Union (EU) and United States of America (USA), and to meet very stringent future automotive emission standards, e.g., Tier 2 program in USA and EURO V in EU. In addition, major challenges to the widespread use of HCCI engines in road applications are discussed in aspects of new catalytic emissions controls to remove high CO and unburned hydrocarbons from such engine-equipped vehicles.

• 비교문화연구
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• 제29권
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• pp.201-232
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• 2012

In the world history in the rein of totalitarianism cultural project of government had been activated, and consequently of it official culture had been organized. But at the same time on the other side of it people('narod'), who didn't have cultural means for active expression of own opinion on the reality, had expressed world-view and judgement informally in everyday language. In the literature of autocracy, subjected to censorship, had been expressed and fixed mythically idealized image of sovereign and his works. But in the folklore the image of ruler had been created by liberal fantasy of people. This article examined russian literature and folklore texts of 18 century, when russian people suffered from rapid and dramatic changes, caused by Peter I. Although russian literature of 18 century had gone over to the new literary regime, it still accepted political mechanism as dominant of age, and consequently in the literary texts of this century Peter I was represented as ideal person and great monarch. But various images in folklore texts show that people's opinion on ruler and his activities couldn't be controlled. In other worlds, diverse images of Peter I in folklore texts reflect clear and plain historical consciousness of people. This analysis reveals not only difference between mechanism of idealization of government and historical consciousness of people, but also meaning of cultural memory as indicator of historical reality.

• 간호행정학회지
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• 제4권2호
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• pp.351-361
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• 1998

The purpose of this study was to explore whether there is a point within the range of physical impairment after which the cost of home care exceeds the cost of nursing home care among the elderly who require long-term care. The provision of long-term care for the elderly is a major health policy issue, in part due to the aging of the American population and dramatic increase in health care costs. The framework for this study was guided by Pollak's(1973)model of costs of alternative care settings for the elderly. This study used a retrospective, descriptive correlational design. Physical impairment was measured by the modified Index of Activities of Daily Living(Katz et al. 1963). Cost of care was measured by the average cost per patient per day. The sample for this study included 67 patients receiving long-term care at home from the Long-term Home Health Care Programs (LTHHCPs) and 67 patients receiving long-term care in nursing homes. Data were collected on patient characteristics. including activities of daily living and cognitive impairment. and on the number of physician visits. emergency room visits. and hospitalization from the patient records. For each patient. Medicaid cost data for home care services/or nursing home services were collected from the financial department of each home care agency or nursing home. The living costs and informal care costs were estimated for home care patients. The results indicated that the home care sample and the nursing home sample were similar in terms of gender. ethnic background. and marital status. The elderly patients in the home care sample were: however. younger and less physically impaired than those in the nursing home sample. The hypotheses of this study were supported: For elderly persons with physical impairment scores below 12(possible range of 0 to 14), cost of care was lower in home care than in the nursing home care setting. However, for elderly persons with physical impairment scores above 12. the cost of care was higher in home care than in the nursing home care setting. Thus. in this sample for elderly patients with extreme physical impairment, the cost of home care exceeded the cost of nursing home care.

• 한국자기공명학회논문지
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• 제13권1호
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• pp.27-34
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• 2009

HP (2-20), a 19-residue peptide derived from the N-terminus of Helicobacter pylori Ribosomal Protein L1, has antimicrobial activity but is not cytotoxic to human erythrocytes. Previously, we have synthesized several analogue peptides to investigate the effects of substitutions on the structure and antimicrobial activity. Substitution of $Gln^{16}$ and $Asp^{18}$ with Trp (Anal 3) caused a dramatic increase in bacterial and fungal lytic activities. In this study, analogue peptides were synthesized to investigate the effects of substitution of Gin and Asp with Phe (Anal 6) or Tyr (Anal 7) in HP (2-20) on its structure and antimicrobial activity. Substitution of Gin and Asp with hydrophobic aromatic residues at position 16 and 18 of HP (2-20) caused increase in antibiotic activity without hemolytic effect. Substitution of Gin and Asp with Trp and Try increased antibiotic activity of HP (220) twice more compared to substitution with Phe. The tertiary structures of Anal 6 and Anal 7 in SDS micelles has been investigated using NMR spectroscopy. The structures revealed that substitutions of the aromatic residues at C-terminus resulted in longer and well defined alpha-helix and improved their antibacterial activities

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제6권5호
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• pp.1445-1462
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• 2012

While botnets are used for various malicious activities, it is well known that they are widely used for email spam. Though the spam filtering systems currently in use block IPs that send email spam, simply blocking the IPs of zombie PCs participating in a botnet is not enough to prevent the spamming activities of the botnet because these IPs can easily be changed or manipulated. This IP blocking is also insufficient to prevent crimes other than spamming, as the botnet can be simultaneously used for multiple purposes. For this reason, we propose a system that detects botnets and zombie PCs based on email spam analysis. This study introduces the concept of "group pollution level" - the degree to which a certain spam group is suspected of being a botnet - and "IP pollution level" - the degree to which a certain IP in the spam group is suspected of being a zombie PC. Such concepts are applied in our system that detects botnets and zombie PCs by grouping spam mails based on the URL links or attachments contained, and by assessing the pollution level of each group and each IP address. For empirical testing, we used email spam data collected in an "email spam trap system" - Korea's national spam collection system. Our proposed system detected 203 botnets and 18,283 zombie PCs in a day and these zombie PCs sent about 70% of all the spam messages in our analysis. This shows the effectiveness of detecting zombie PCs by email spam analysis, and the possibility of a dramatic reduction in email spam by taking countermeasure against these botnets and zombie PCs.

• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.277-288
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• 2017

Rhizomucor miehei NRRL 5282 and Rhizopus oryzae NRRL 1526 can produce lipases with high synthetic activities in wheat bran-based solid-state culture. In this study, the purification and biochemical characterization of the lipolytic activities of these lipases are presented. SDS-PAGE indicated a molecular mass of about 55 and 35 kDa for the purified R. miehei and Rh. oryzae enzymes, respectively. p-Nitrophenyl palmitate (pNPP) hydrolysis was maximal at $40^{\circ}C$ and pH 7.0 for the R. miehei lipase, and at $30^{\circ}C$ and pH 5.2 for the Rh. oryzae enzyme. The enzymes showed almost equal affinity to pNPP, but the $V_{max}$ of the Rh. oryzae lipase was about 1.13 times higher than that determined for R. miehei using the same substrate. For both enzymes, a dramatic loss of activity was observed in the presence of 5 mM $Hg^{2+}$, $Zn^{2+}$, or $Mn^{2+}$, 10 mM N-bromosuccinimide or sodium dodecyl sulfate, and 5-10% (v/v) of hexanol or butanol. At the same time, they proved to be extraordinarily stable in the presence of n-hexane, cyclohexane, n-heptane, and isooctane. Moreover, isopentanol up to 10% (v/v) and propionic acid in 1 mM concentrations increased the pNPP hydrolyzing activity of R. miehei lipase. Both enzymes had 1,3-regioselectivity, and efficiently hydrolyzed p-nitrophenyl (pNP) esters with C8-C16 acids, exhibiting maximum activity towards pNP-caprylate (R. miehei) and pNP-dodecanoate (Rh. oryzae). The purified lipases are promising candidates for various biotechnological applications.

• 비교문화연구
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• 제38권
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• pp.115-144
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• 2015

The purpose of this study is to look into the support activities of the 17th century spanish plays which had been taken in various paths in terms of responses of the then-public who had been enthusiastic about great plays and artworks. Such a phenomenon clearly appeared in the open-air theater 'corral' where 'Teatro nacional' was performed at that time. Therefore, this study tries to investigate the functional relation between the public's responses and the prosperity of then-plays from the concrete standpoint of Lope's public-oriented plays. Although Lope's plays basically include system-protection ideology, they are thoroughly based on the public's support and popularity. In short, at that time, Lope's dramatic reform and entailing remarkable hit and success were made possible through thorough compliance with the public's preference and thereby the public's absolute support and affection. The tendency of his public-oriented plays appears in two aspects. One is the common people persecuted and punishment of a wicked man of power. The typical plays in point are Lope's Perib??ez y el comendador de Oca?a and El mejor alcalde, el rey. Through the plays, the playwright Lope not only implemented praise of absolute monarch very effectively, but soothed the wounded heart of the common people who had suffered pains due to their unfair and lowly social status. In this way, his plays might give senses of reliefs and satisfaction to most viewers who identified with the common people in the plays. The other one is the decisive raise of status of an exemplary commoner, the subject matter which had been used by Lope as one of useful means to show the public-oriented tendency of his plays. The cases in point are Los Tellos de Meneses and its succeeding work Valor, fortuna y lealtad. The two-part work of Lope is about the hard journey, ups and downs, and ascent to the nobility of Tello, an exemplary and rich farmer, and hits young son Tello. Through the two-part play, Lope demonstrated how an exemplary and good-mannered commoner rose to the nobility and thereby gave vicarious satisfaction to the then-public and received a lot of praise from them. As mentioned earlier, through his public-oriented plays, Lope not only satisfied the public's desires and achieved his realistic goal of big success. Not all Lope's plays are public-oriented. Nevertheless, the popular success of 'Teatro nacional' made by Lope and his followers is based on the public's preference and the plays' dramatic responses to their desires, and the strategic elaboration to comfort the public suffering lot of pains in daily life.

• 시큐리티연구
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• 제36호
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• pp.227-253
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• 2013

오늘날 범죄에 대한 위협은 전 세계적인 이슈가 되고 있다. 대표적으로 테러, 조직화된 범죄, 범국가적 범죄가 바로 그것이다. 현대사회의 범죄는 전통적 범죄(살인, 강도, 강간, 방화)와는 다르게 언제 어디서 어떻게 일어날지 특정화할 수 없다. 이는 치안환경의 변화에 기인한 결과인데 현대의 범죄위협은 불특정 다수를 목표로 하는 테러위험과 같은 산발적 특성을 가지고 있으며, 조직적 범죄와 같은 체계적 현상이 대두되고 있고, 범국가적 범죄와 같은 광범위적 범죄현상에 대처해야 하는 치안환경으로 변화했기 때문이다. 이와 관련해 2001년에 일어난 9.11사건은 치안환경의 극적인 변화를 보여준 사례로 볼 수 있다. 테러 이후 미국은 물론 전 세계의 범죄를 다루는 관련기관에게 범죄정보의 수집과 관리, 활용에 대해 다시 한 번 고민하게 되는 계기를 제공하였다. 그 결과 범죄정보를 보다 효율적으로 수집하고 관리하기 위한 경찰활동의 변화들이 일어났는데 영미국가를 중심으로 나타난 "정보 지향적 경찰활동(ILP)"이 바로 그것이다. 이는 범죄문제를 해결하기 위해 정보를 수집하고 가공함으로써 보다 직접적으로 범죄를 관리하기 위한 업무체계(framework)를 반영하는 시스템으로 범죄위협을 최소화하는 경찰활동을 지향하고 있다. 치안 환경변화가 세계적 공동화 현상으로 일어나고 있는 만큼, 우리나라의 경우도 이와 같은 치안환경의 변화에 따라 정보 주도형 경찰활동을 지향해야할 필요성이 커졌다. 이에 따라, 본 연구에서는 영미국가의 정보 주도형 경찰활동을 살펴봄으로써 도입 방안과 논의사항을 제시하는데 중점을 두어 연구가 이루어졌다.

• 항공우주정책ㆍ법학회지
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• 제24권1호
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• pp.153-194
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• 2009

우주활동의 증가와 더불어 이는 지구상의 인류와 환경에 대한 지속적인 위협요인이 되고 있으며 그중에서도 우주활동과 관련된 환경문제로서 가장 심각한 것으로 파악되고 있는 것이 우주폐기물의 문제이다. 이는 다른 우주 물체에 대해서도 커다란 위협이 될 뿐 아니라 지구의 대기권으로 재진입하여 인간이 통제(control)할 수 없는 상태에서 지구상에 낙하될 때에 인간의 생명과 재산상에 커다란 위험을 초래하게 된다. 현재 우주활동과정에서의 우주공간이나 지구를 포함한 천체에 대한 환경보호와 그 대처를 위한 제도적 장치는 아직 미흡하며 이를 위한 우주국제법적 해결이 모색되고 있고 부분적으로는 우주환경오염을 방지하고자 하는 규정이 일부 조약에 도입되기도 하였다. UN COPUOS를 중심으로 우주조약이나 책임협약 및 달 협정뿐만 아니라 등록협약의 관련조항에서도 가능한 위험을 최소화하기 위한 규정을 두고 있음은 주지의 사실이다. 그밖에도 부분적 핵실험 금지조약이나 핵무기비확산 조약 그리고 환경변화기술의 군사적 사용금지협약 등을 통해서도 우주나 지구자연의 평화적인 이용에 대한 장애요인을 제거하기 위한 직,간접적인 법적 장치가 마련되어 있는 것으로 평가된다. 특히 별도의 법체계를 정립하는 것이 필요하다는 문제와 관련하여 ILA는 일찍이 1986년 세계국제법협회 (ILA) 서울 총회에서 의제로 제안된 이래로 8년여에 걸쳐 여러 차례 각국에서의 회기를 거쳐 이 문제에 대한 지속적인 논의를 해왔던 바, 그 결과 동 협회는 1994년 Buenos Aires 회의에서 최종 초안인 "우주 폐기물에 기인되는 손해로부터 우주 내지 자구환경을 보호하기 위한 국제문서초안"을 작성하여 COPUOS에 제출한 바 있다. 향후 이것은 COPUOS가 의제로 채택하여 기술분과나 법률분과소위원회에서 논의를 할 때 기초적인 검토 자료로서 중요한 역할을 할 것으로 사료되며 동위원회에서 이를 충분히 심의한 후 사안의 심각성에 비추어 조속한 결실을 맺게 되도록 노력해야 할 것이다.