• Journal of Ginseng Research
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• v.31 no.1
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• pp.1-13
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• 2007

We found that the main bacterial metabolite M1 is an active component of orally administered protopanxadiol-type ginsenosides, and that the anti-metastatic effect by oral administration of ginsenosides may be primarily mediated through the inhibition of tumor invasion, migration and growth of tumor cells by their metabolite M1. Pharmacokinetic study after oral administration of ginsenoside Rb1 revealed that M1 was detected in serum for 24 h by HPLC analysis but Rb1 was not detected. M1, with anti-metastatic property, inhibited the proliferation of murine and human tumor cells in a time- and concentration-dependent manner in vitro, and also induced apoptotic cell death (the ladder fragmentation of the extracted DNA). The induction of apoptosis by M1 involved the up-regulation of the cyclin-dependent kinase(CDK) inhibitor $p27^{Kip1}$ as well as the down-regulation of a proto-oncogene product c-Myc and cyclin D1 in a time-dependent manner. Thus, M1 might cause the cell-cycle arrest (G1 phase arrest) in honor cells through the up/down-regulation of these cell-growth related molecules, and consequently induce apoptosis. The nucleosomal distribution of fluorescence-labeled M1 suggests that the modification of these molecules is induced by transcriptional regulation. Tumor-induced angiogenesis (neovascularization) is one of the most important events concerning tumor growth and metastasis. Neovascularization toward and into tumor is a crucial step for the delivery of nutrition and oxygen to tumors, and also functions as the metastatic pathway to distant organs. M1 inhibited the tube-like formation of hepatic sinusoidal endothelial (HSE) cells induced by the conditioned medium of colon 26-L5 cells in a concentration-dependent manner. However, M1 at the concentrations used in this study did not affect the growth of HSE cells in vitro.

• Fire Science and Engineering
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• v.28 no.3
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• pp.10-19
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• 2014

The fast evacuation from fire floors to evacuation floors in high-rise building fires can minimize the human damage. In this study, an evacuation instrument, which are applicable to the high-rise buildings of adaptable escape mechanisms by the current NFSC 301 (national fire safety code 301), were selected to analyze the applicability in the high-rise buildings over 11th floor through the site adaptability test. The results of the site test were as follows. The elevator type evacuation instrument of new concept developed as a new technology by compensating the defect of evacuation instrument limiting in the high-rise buildings over 11th floor had completed the stability test and the performance certification test in fire stations, which there were no problems in the introduction of the elevator type evacuation instrument as an escape mechanism in the high-rise buildings. The elevator type evacuation instrument using escapers' weight without using electric power was an escape mechanism that many people could evacuate in a short period of time when a fire broke out in the high-rise buildings. In particular, The elevator type evacuation instrument operated by nonpower had the adaptability as a customized escape mechanism considering user characteristics in the buildings for the disabled or patients with an advanced disease.