• The Plant Pathology Journal
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• v.30 no.4
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• pp.450-455
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• 2014

This study sought to control the root-knot nematode (RKN) Meloidogyne incognita using benign organo-chemicals. Second-stage juveniles (J2) of RKN were exposed to dilutions (1.0%, 0.5%, 0.2%, and 0.1%) of acetic acid (AA), lactic acid (LA), and their mixtures (MX). The nematode bodies were disrupted severely and moderately by vacuolations in 0.5% of MX and single organic acids, respectively, suggesting toxicity of MX may be higher than AA and LA. The mortality of J2 was 100% at all concentrations of AA and MX and only at 1.0% and 0.5% of LA, which lowered slightly at 0.2% and greatly at 0.1% of LA. This suggests the nematicidal activity of MX may be mostly derived from AA together with supplementary LA toxicity. MX was applied to chili pepper plants inoculated with about 1,000 J2, for which root-knot gall formations and plant growths were examined 4 weeks after inoculation. The root gall formation was completely inhibited by 0.5% MX and standard and double concentrations of fosthiazate; and inhibited 92.9% and 57.1% by 0.2% and 0.1% MX, respectively. Shoot height, shoot weight, and root weight were not significantly ($P{\leq}0.05$) different among all treatments and the untreated and non-inoculated controls. All of these results suggest that the mixture of the organic acids may have a potential to be developed as an eco-friendly nematode control agent that needs to be supported by the more nematode control experiments in fields.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2004.11a
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• pp.9-10
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• 2004

We developed a new panel of markers for the characterization of chicken PGCs. The results of immunostaining demonstrated that anti-SSEA-3, anti-SSEA-4, anti-integrin 6, and anti-integrin 1 antibodies. and STA and DBA bound specifically to chicken PGCs. These reagents could be used to characterize chicken PGCs together with conventional marker reagents such as PAS and anti-SSEA-1 antibody. We also showed that double staining of PGCs with the newly developed markers was feasible, which might contribute to rapid detection and accurate characterization of chicken PGCs.

• Journal of Plant Biotechnology
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• v.7 no.2
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• pp.87-95
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• 2005

The tomato ripening associated membrane protein (TRAMP) (Fray et al., 1994) is a member of the major intrinsic protein (MIP) family, defined as channels facilitating the passage of water and small solutes through membranes. During normal fruit ripening the TRAMP mRNA levels were increased whereas the expression levels of TRAMP in low ethylene ACO1-sense suppressed lines, Nr and rin fruits, were lower than at the breaker stage of wild type fruit. TRAMP mRNA is inhibited by $LaCl_3$, which is an inhibitor of $Ca^{2+}$-stimulated responses, treatment but drought condition did not affect TRAMP expression. The levels of TRAMP mRNA transcripts were substantially higher in the dark treated seedlings and fruits. These suggest that TRAMP function as a water channel may be doubted because of several reasons; no water content was changed during ripening in wild type, antisense and overexpression lines, TRAMP expression under light condition was lower than dark condition and TRAMP expression was not changed in drought condition. Co-suppression plant, 3588 was one of sense suppression lines, which contain CaMV 35S promoter and sense pNY507 cDNA, produced small antisense RNA, approximately 21-25 nucleotides in length, mediated post-transcriptional gene silencing. Therefore, TRAMP expression was inhibited by small antisense and multiple copies might induce gene silencing without any production of double strand RNA. Total seven selected volatile productions, isobutylthiazole, 6-methyl-5-hepten-2-one, hexanal, hexenal methylbutanal, hexenol, and methylbutanol, were highly reduced in sense line whereas total volatile production was increased in TRAMP antisense line. These results suggested TRAMP might change volatile related compounds.

• Journal of Preventive Medicine and Public Health
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• v.5 no.1
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• pp.43-48
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• 1972

5 bed sand filter, applying biological oxidation, was designed and studied on the treatment of water works. Never using any coagulant agent (drugs), which may cause water pollution in pre-treatment of head water, the auther attempt a high rate filtration by the microorganism (nitrofication bacteria) end plant which populate in multi layer sand beds. The result are as follows : In order to evaluate the oxygen effect on filtration, oxygen was injected in aeration tank attached to each filter tank while filtration, and $NH_3$ was tested as a representaiive ingredient. It was found out that the aeration method was more effective, with over 33% of $NH_3$ removal capacity, than the anerobic and this 5 bed filter showed double removal capacity of $NH_3$ by comparing with conventional sand bed (2 stage bed). According to the examination of two kind of head water, pre-treated with coagulant agent and activated carton, the filtration capacity was affected by the polluted condition of head water, resulting that lower value of pollution and slower velocity of filtration showed more efficiency of $NH_3$ removal. In this experiment $NH_3$ content tested in treated water had a fairly good correlation with others.

• Explosives and Blasting
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• v.30 no.1
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• pp.37-51
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• 2012

In this case of "Seongnam~Yeoju double-lanes railroad construction", there were safety facilities which were concerned about damages from vibration and noise. In the project design stage, the rock-splitter method was designed to prevent them. The electronic blasting was considered to improve construction speed and economic value as an alternative tunnelling method, complying with the site's vibration criteria(cowhouse : 0.09cm/sec, residence : 0.2cm/sec). In the environment evaluation report of the eDev, tunnel electronic blasting systems, the blasting pollutions can be managed by the electronic blasting method. The results were successfully conducted with high speed construction without any damages to adjacent facilities.

• Journal of Korean Tunnelling and Underground Space Association
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• v.15 no.3
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• pp.321-331
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• 2013

This paper describes the evaluation of shield TBM disc cutter penetration depth in practice. In this study the disc cutter penetration depth used to design the excavation speed of tunnel is reviewed. The characteristics of ground encountered in the investigation site are analysed and evaluated. The shield TBM used in the field is reviewed to verify the applicability of machine in the site. The thrust and torque capacities of each TBM disc cutter are also evaluated. Based on the field data, the excavation volume and speed are re-analysed to evaluate the disc cutter penetration depth used in the design stage. It is clearly found that the design value of disc cutter penetration depth needs to modify when estimation of the TBM capacities in very hard rock formation ($S_c$ >150 MPa).

• Asian Journal of Atmospheric Environment
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• v.6 no.1
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• pp.14-22
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• 2012

This paper is focused on the comprehensive and detailed interpretation for the chemical transformation of individual Asian dust (hereafter called "AD") particles during long-range transport from source regions to receptor area. A multi-stage particle sampler was operated at a ground-based site in Taean, Korea directly exposed to the outflow of air masses from China during AD period in April 2003. Both quantitative and qualitative analyses for size-classified individual particles were carried out by a microbeam X-ray fluorescence (XRF) method and a microbeam Particle Induced X-ray Emission (micro-PIXE), respectively. Among major characteristic elements, the elemental masses of soil derived components, sulfur, and chloride varied as a function of particle size showing the monomodal maximum with a steeply increasing at 3.3-4.7 ${\mu}m$ particle size. Although the details on chemical composition of AD particle collected on a straight line from source area to our ground-based site are needed, a large amount of Cl coexisted in and/or on AD particles suggests that AD particles collected in the present study might be actively engaged in chemical transformation by sea-salt and other Cl containing pollutants emitted from the China's domestic sources. Through the statistical analyses it was possible to classify individual AD particles into six distinct groups. The internally mixed AD particles with Cl, which has various sources (e.g., sea-salt, coal combustion origin HCl, gaseous HCl derived from the adsorption of acids to sea-salt, and Cl containing man-made particles) were thoroughly fractionated by the elemental spectra drivened by the double detector system of micro-PIXE.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.4
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• pp.395-400
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• 1983

Rock trout, Agrammus agrammus(Temminck et Schlegel) is commonly found at the coast of Korean waters. Mature adult of the rock trout were collected at the shore of Dongbaeksom in Pusan during the period from Nov. 18 to Nov. 25, 1983 through know the early developmental characters. The authors carried out the artificial insemination in the laboratory on Nov. 24, 1982, and roared the eggs and the hatched larvae. The eggs of this species are demersal and adhesive, and their diameter were varied within 1.92-2.14mm. The color of yolk is light blue in the early developmental stage, and then turned to orange before hatching out. The yolk contained numerous tiny oil globules. Hatchig toot plnace in ca. 463 hours after insemination at $11.8-17.9^{\circ}C$ of water temperature. The newly hatched larvae were elongate in share and 6.55-8.20 mm in total length with 12+26=48 myomers. Many branched melanophores were distributed on the supra-orbital, dorsal double body contour, and peritoneal, and also under the notochord of the tail.

• The Korean Journal of Physiology and Pharmacology
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• v.13 no.3
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• pp.251-256
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• 2009

Previous observations suggest that Bis, a Bcl-2-binding protein, may playa role the neuronal and glial differentiation in vivo. To examine this further, we investigated Bis expression during the in vitro differentiation of P19 embryonic carcinoma cells induced by retinoic acid (RA). Western blotting and RT-PCR assays showed that Bis expression was temporarily decreased during the free floating stage and then began to increase on day 6 after the induction of differentiation. Double immunostaining indicated that Bis-expressing cells do not express several markers of differentiation, including NeuN, MAP-2 and Tuj-1. However, some of the Bis-expressing cells also were stained with GFAP-antibodies, indicating that Bis is involved glial differentiation. Using an shRNA strategy, we developed bis-knock down P19 cells and compared them with control P19 cells for the expression of NeuroD, Mash-1 and GFAP during RA-induced differentiation. Among these, only GFAP induction was significantly attenuated in Pl9-dnbis cells and the population showing GFAP immunoreactivity was also decreased. It is noteworthy that distribution of mature neurons and migrating neurons was disorganized, and the close association of migrating neuroblasts with astrocytes was not observed in P19-dnbis cells. These results suggest that Bis is involved in the migration-inducing activity of glial cells.

• Journal of Korean Physical Therapy Science
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• v.12 no.4
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• pp.33-41
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• 2005

The purpose of this report was to describe the gait pattern and parameters of the complicated bilateral amputee with right transtibial and left tarsometatarsal amputation. Using a Vicon 370 three dimensional gait analysis system, the gait analysis was performed at pre and post-test. Treadmill Training with 15 degree, incline was practiced for 8weeks, 3times per week. In linear parameters, the Velocity, Stride length and Single limb support were increased than pre-test. but Cadence and Double limb support were less post-test than pre-test. In kinematics, the maximal pelvic tilt angle showed right side $21.87^{\circ}$, left side $20.67^{\circ}$ at pre-swing phase, and decreased as compared with pre-test. Especially, the inimal hip flexion angle showed right side $-6.83^{\circ}$, left side $1.52^{\circ}$ at pre-swing phase and increased as compared with pre-test. The maximal knee flexion angle disclosed right side $2.66^{\circ}$, left side $21.71^{\circ}$ at stance phase, and decreased as compared with pre-test. In kinetics, the hip extension moment on initial contact stage was right side 0.938NM/Kg, left side 0.09NM/Kg, which was impaired compared with normal person.