Browse > Article

Bis Is Involved in Glial Differentiation of PI9 Cells Induced by Retinoic Acid  

Yoon, Jung-Sook (Departments of Biomedical Science, The Catholic University of Korea)
Lee, Mun-Yong (Departments of Anatomy, Graduate School, College of Medicine, The Catholic University of Korea)
Lee, Jae-Seon (Division of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences)
Park, Chan-Sun (Bioindustry Research Center, Korea Research Institute Bioscience and Biotechnology)
Youn, Ho-Joong (Internal Medicine, The Catholic University of Korea)
Lee, Jeong-Hwa (Biochemistry, College of Medicine, The Catholic University of Korea)
Publication Information
The Korean Journal of Physiology and Pharmacology / v.13, no.3, 2009 , pp. 251-256 More about this Journal
Abstract
Previous observations suggest that Bis, a Bcl-2-binding protein, may playa role the neuronal and glial differentiation in vivo. To examine this further, we investigated Bis expression during the in vitro differentiation of P19 embryonic carcinoma cells induced by retinoic acid (RA). Western blotting and RT-PCR assays showed that Bis expression was temporarily decreased during the free floating stage and then began to increase on day 6 after the induction of differentiation. Double immunostaining indicated that Bis-expressing cells do not express several markers of differentiation, including NeuN, MAP-2 and Tuj-1. However, some of the Bis-expressing cells also were stained with GFAP-antibodies, indicating that Bis is involved glial differentiation. Using an shRNA strategy, we developed bis-knock down P19 cells and compared them with control P19 cells for the expression of NeuroD, Mash-1 and GFAP during RA-induced differentiation. Among these, only GFAP induction was significantly attenuated in Pl9-dnbis cells and the population showing GFAP immunoreactivity was also decreased. It is noteworthy that distribution of mature neurons and migrating neurons was disorganized, and the close association of migrating neuroblasts with astrocytes was not observed in P19-dnbis cells. These results suggest that Bis is involved in the migration-inducing activity of glial cells.
Keywords
Bis; GFA; Gliogenesis; Neuronal differentiation; P19;
Citations & Related Records

Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 1
연도 인용수 순위
1 Choi JS, Lee JH, Shin YJ, Lee JY, Yun H, Chun MH, Lee MY. Transient expression of Bis protein in the midline glial structure in the developing rat brainstem and spinal cord. Cell Tissue Res 337: 27−36, 2009   DOI   PUBMED   ScienceOn
2 Hatten ME. Central nervous system neuronal migration. Annu Rev Neurosci 22: 511−539, 1999   DOI   PUBMED   ScienceOn
3 Iwasaki M, Homma S, Hishiya A, Dolezal SJ, Reed JC, Takayama S. BAG3 regulates motility and adhesion of epithelial cancer cells. Cancer Res 67: 10252−10259, 2007   DOI   ScienceOn
4 Jing XT, Wu HT, Wu Y, Ma X, Liu SH, Wu YR, Ding XF, Peng XZ, Qiang BQ, Yuan JG, Fan WH, Fan M. DIXDC1 promotes retinoic acid-induced neuronal differentiation and inhibits gliogenesis in P19 cells. Cell Mol Neurobiol 29: 55−67, 2009   DOI   ScienceOn
5 Jones-Villeneuve EM, Rudnicki MA, Harris JF, McBurney MW. Retinoic acid-induced neural differentiation of embryonal carcinoma cells. Mol Cell Biol 3: 2271−2279, 1983   PUBMED   ScienceOn
6 Kassis JN, Guancial EA, Doong H, Virador V, Kohn EC. CAIR-1/ BAG-3 modulates cell adhesion and migration by downregulating activity of focal adhesion proteins. Exp Cell Res 312: 2962−2971, 2006   DOI   ScienceOn
7 Lim DA, Alvarez-Buylla A. Interaction between astrocytes and adult subventricular zone precursors stimulates neurogenesis. Proc Natl Acad Sci U S A 96: 7526−7531, 1999   DOI   ScienceOn
8 Romano MF, Festa M, Pagliuca G, Lerose R, Bisogni R, Chiurazzi F, Storti G, Volpe S, Venuta S, Turco MC, Leone A. BAG3 protein controls B-chronic lymphocytic leukaemia cell apoptosis. Cell Death Differ 10: 383−385, 2003   DOI   ScienceOn
9 Santiago MF, Liour SS, Mendez-Otero R, Yu RK. Glial-guided neuronal migration in P19 embryonal carcinoma stem cell aggregates. J Neurosci Res 81: 9−20, 2005   DOI   ScienceOn
10 Takayama S, Xie Z, Reed JC. An evolutionarily conserved family of Hsp70/Hsc70 molecular chaperone regulators. J Biol Chem 274: 781−786, 1999   DOI   ScienceOn
11 Lee MY, Kim SY, Shin SL, Choi YS, Lee JH, Tsujimoto Y. Reactive astrocytes express bis, a bcl-2-binding protein, after transient forebrain ischemia. Exp Neurol 175: 338−346, 2002   DOI   ScienceOn
12 Lee JH, Takahashi T, Yasuhara N, Inazawa J, Kamada S, Tsujimoto Y. Bis, a Bcl-2-binding protein that synergizes with Bcl-2 in preventing cell death. Oncogene 18: 6183−6190, 1999   DOI   ScienceOn
13 Carra S, Seguin SJ, Lambert H, Landry J. HspB8 chaperone activity toward poly(Q)-containing proteins depends on its association with Bag3, a stimulator of macroautophagy. J Biol Chem 283: 1437−1444, 2008   DOI   ScienceOn
14 Hong S, Heo J, Lee S, Heo S, Kim SS, Lee YD, Kwon M. Methyltransferase-inhibition interferes with neuronal differentiation of P19 embryonal carcinoma cells. Biochem Biophys Res Commun 377: 935−940, 2008   DOI   ScienceOn
15 Pagliuca MG, Lerose R, Cigliano S, Leone A. Regulation by heavy metals and temperature of the human BAG-3 gene, a modulator of Hsp70 activity. FEBS Lett 541: 11−15, 2003   DOI   ScienceOn
16 Bonelli P, Petrella A, Rosati A, Romano MF, Lerose R, Pagliuca MG, Amelio T, Festa M, Martire G, Venuta S, Turco MC, Leone A. BAG3 protein regulates stress-induced apoptosis in normal and neoplastic leukocytes. Leukemia 18: 358−360, 2004   DOI   ScienceOn
17 Chen L, Wu W, Dentchev T, Zeng Y, Wang J, Tsui I, Tobias JW, Bennett J, Baldwin D, Dunaief JL. Light damage induced changes in mouse retinal gene expression. Exp Eye Res 79: 239−247, 2004   DOI   ScienceOn
18 Choi JS, Lee JH, Kim HY, Chun MH, Chung JW, Lee MY. Developmental expression of Bis protein in the cerebral cortex and hippocampus of rats. Brain Res 1092: 69−78, 2006   DOI   ScienceOn
19 Liour SS, Yu RK. Differentiation of radial glia-like cells from embryonic stem cells. Glia 42: 109−117, 2003   DOI   ScienceOn
20 Park HJ, Choi JS, Chun MH, Chung JW, Jeon MH, Lee JH, Lee MY. Immunohistochemical localization of Bis protein in the rat central nervous system. Cell Tissue Res 314: 207−214, 2003   DOI   ScienceOn
21 Jones-Villeneuve EM, McBurney MW, Rogers KA, Kalnins VI. Retinoic acid induces embryonal carcinoma cells to differentiate into neurons and glial cells. J Cell Biol 94: 253−262, 1982   DOI   ScienceOn
22 Doong H, Price J, Kim YS, Gasbarre C, Probst J, Liotta LA, Blanchette J, Rizzo K, Kohn E. CAIR-1/BAG-3 forms an EGF- regulated ternary complex with phospholipase C-gamma and Hsp70/Hsc70. Oncogene 19: 4385−4395, 2000   DOI   ScienceOn
23 Jacobs AT, Marnett LJ. HSF1-mediated BAG3 expression attenuates apoptosis in 4-hydroxynonenal-treated colon cancer cells via stabilization of anti-apoptotic Bcl-2 proteins. J Biol Chem 284: 9176−9183, 2009   DOI   ScienceOn