• Title/Summary/Keyword: Double stage

Search Result 546, Processing Time 0.035 seconds

The Structual Restoration on Gyeongju-Style Piled Stone-Type Wooden Chamber Tombs (경주식 적석목곽묘의 구조복원 재고)

  • Gweon, Yong Dae
    • Korean Journal of Heritage: History & Science
    • /
    • v.42 no.3
    • /
    • pp.66-87
    • /
    • 2009
  • The definition of the structure of wooden chamber tomb(piled stone-type) is as follows. It is a tomb with wooden chamber, and stones were piled on top of the wooden chamber, and then a wooden structure was placed on top of the piled stones, and more stones were piled on top of the wooden structure, and sealed with clay. Of course this definition can vary according to periods, the buried, etc. Gyeongju-style piled stone type wooden chamber tombs have some distinguished characteristics compared to general definition of piled stone type wooden chamber tombs. Outside the wooden chamber, either stone embankments or filled-in stones were layed out, and pilet-in stones are positioned right above the wooden chamber, and almost every class used this type, and finally, it is exclusively found in Gyeongju area. First generations of this Gyeongju-style piled stone type wooden chamber tombs appeared in first half of 5th century. These tombs inherited characteristics like ground plan, wooden chamber, double chamber(inner chamber and outer chamber), piled stones, burial of the living with the dead, piled stones, from precedent wooden chamber tombs. However these tombs have explicit new characteristics which are not found in the precedent wooden chamber tombs such as stone embankments, wooden pillars, piled stones(above ground level), soil tumuluses. stone embankments and wooden pillars are exclusively found on great piled stone type above-ground level wooden chamber tombs such as the Hwangnamdaechong(皇南大塚). Stone embankments, wooden pillars, piled stones(above ground level) are all elements of building process of soil tumuluses. stone embankments support outer wall of above-ground level wooden chambers and disperse the weight of tumuluses. Wooden pillars functioned as auxiliary supports with wooden structures to prevent the collapse of stone embankments. Piled stones are consists of stones of regular size, placed on the wooden structure. And after the piled stones were sealed with clay, tumulus was built with soil. Piled stones are unique characteristics which reflects the environment of Gyeongju area. Piled stone type wooden chamber tombs are located on the vast and plain river basin of Hyeongsan river(兄山江). Which makes vast source of sands and pebbles. Therefore, tumulus of these tombs contains large amount of sands and are prone to collapse if soil tumulus was built directly on the wooden structure. Consequently, to maintain external shape of the tumulus and to prevent collapse of inner structure, piled stones and clay-sealing was made. In this way, they can prevent total collapse of the tombs even if the tumulus was washed away. The soil tumulus is a characteristic which emerges when a nation or political entity reaches certain growing stage. It can be said that after birth of a nation, growing stage follows and social structure will change, and a newly emerged ruling class starts building new tombs, instead of precedent wooden chamber tombs. In this process, soil tumulus was built and the size and structure of the tombs differ according to the ruling class. Ground plan, stone embankments, number of the persons buried alive with the dead, quantity and quality of artifacts reflect social status of the ruling class. In conclusion, Gyeongju-style piled stone type wooden chamber tombs emerged with different characteristics from the precedent wooden chamber tombs when Shilla reached growing stage.

A Study on Views of Vital Capital in Film (영화 <기생충>에 나타난 생명자본의 관점에 관한 연구)

  • Kang, Byoung-Ho
    • Journal of Korea Entertainment Industry Association
    • /
    • v.15 no.3
    • /
    • pp.75-88
    • /
    • 2021
  • The film won the Golden Palm Award at the Cannes Film Festival, and received the Academy Award for a non-English-speaking film in February 2020, respectively. It has received a monumental evaluation in the world film history. Overall, this film is about class conflict, and critics evaluate the theme of the film as "badly twisted class gap" and "anger from class." The film expresses an intrinsic conflict embodied in culture as a "tragedy in which no bad person appears," rather than the dichotomous composition of the classical class struggle from Marxism. In other words, this can be seen as expressing the substrated class relationship of the modern society that Pierre Bourdieu had argued. This film has been focused as a controversial target under Korea society with excess of ideology. Politics used to adopt the keyword, 'parasite', for political disputes not only in culture contents world. Paradoxically socialism China did not allow to release film 'Parasite.' On the other hand, Lee O-Yong argues that the movie "Parasite" does not look at social phenomena through a dichotomous perspective, but is viewed through a "double perspective" and evaluates that it does not lose eyes looking at humans through tension. This view is based upon 'Vital Capitalism'. Lee. O-Yong looks at the movie "Parasite" from the perspective of "Vital Capitalism". The theory of Vital Capitalism does not seek to find the root of historical development in class struggle conflicts, but rather figuring out history and society pays attention onto the intrinsic characteristics of life, Topophilia, Neophilia, and Biophilia. Lee Eo-ryeong argues that the development of civilization theory evolved from the stage of Hobbes' Darwinism or predatism to the stage of host vs. parasite of Michel Serres, and onto the stage of Margulis's 'Win-Win (inter-dependence)'. In this paper, after overview of vital capital concept and preceeding research, re-interpretations were tried onto scenes based upon fields from habitus, culture capital. This exploration looks for a alternative for excess of ideology in Korea society.

Decentralized Composting of Garbage in a Small Composter for Dwelling House;III. Laboratory Composting of the Household Garbase in a Small Bin with Double Layer Walls (가정용 소형 퇴비화용기에 의한 부엌쓰레기의 분산식 퇴비화;III. 실험실조건에서 이중벽 소형 용기에 의한 퇴비화 연구)

  • Seo, Jeoung-Yoon;Joo, Woo-Hong
    • Korean Journal of Environmental Agriculture
    • /
    • v.14 no.2
    • /
    • pp.232-245
    • /
    • 1995
  • The garbage from the dwelling house was composted in two kinds of small composter in the laboratory, and the possibility of garbage composting was examined. The composters were general small. One (type 3) was constructed with the double layer walls and the other (type 4) was the same as the first except for being insulated. Because it was found that type 3 was not available for composting under our meteorological conditions through the winter experiment, only type 4 was tested in spring and summer. The experiment was performed for 8 weeks in each season. The seasonal variation of several components in the compost was evaluated and discussed. The results summarized below were those obtained at the end of the experiment, if the time was not specified. 1) The maximum temperature was $43^{\circ}C$ in winter, $55^{\circ}C$ in spring and $56^{\circ}C$ in summer. 2) The mass was reduced to an average of 63% and the volume reduction was an average of 78%. 3) The density was estimated as 1.5 kg/l in winter and 0.8 kg/l in spring and summer. 4) The water content was not much changed during the composting periods. It was 79.3% in winter, 75.0% in spring and 70.0% in summer. 5) After pH value increased during the first week, it decreased until the second week and increased again continuously thereafter. It reached pH 6.19 in winter, pH 7.59 in spring and pH 8.69 in summer. 6) The faster the organic matter was decomposed, the greater the ash content increased. The contents of cellulose and lignin increased, but that of hemicellulose decreased during the composting period. 7) Nitrogen contents were in the range of 3.3-6.8% and especially high in summer. After ammonium contents increased at the early stage of the composting period, they decreased. The maximum ammonium-nitrogen content was 2,404mg/kg after 8 weeks in winter, 12,400mg/kg after 3 weeks in spring and 20,718mg/kg after 3 weeks in summer. C/N-ratios decreased with the lapse of composting time, but they were not much changed. Nitrification occurred actively in summer. 8) The contents of volatile and higher fatty acids increased at the early stage of composting and reduced after that. The maximum content of total fatty acid was 9.7% after 6 weeks in winter, 14.8% after 6 weeks in spring and 15.8% after 2 weeks in summer. 9) The contents of inorganic components were not accumulated as composting proceeded. They were in the range of 0.9-4.4% $P_2O_5$, 1.6-2.4% $K_2O$, 2.2-5.4% CaO and 0.30-0.61% MgO. 10) CN and heavy metal contents did not show any tendency. They were in the range of 0.21-14.55mg/kg CN, 11-166mg/kg Zn, 5-65mg/kg Cu, 0.5-10.8mg/kg Cd, 6- 35mg/kg Pb, ND-33 mg/kg Cr and ND-302.04 g/kg Hg.

  • PDF

Estimation of Fresh Weight and Leaf Area Index of Soybean (Glycine max) Using Multi-year Spectral Data (다년도 분광 데이터를 이용한 콩의 생체중, 엽면적 지수 추정)

  • Jang, Si-Hyeong;Ryu, Chan-Seok;Kang, Ye-Seong;Park, Jun-Woo;Kim, Tae-Yang;Kang, Kyung-Suk;Park, Min-Jun;Baek, Hyun-Chan;Park, Yu-hyeon;Kang, Dong-woo;Zou, Kunyan;Kim, Min-Cheol;Kwon, Yeon-Ju;Han, Seung-ah;Jun, Tae-Hwan
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.23 no.4
    • /
    • pp.329-339
    • /
    • 2021
  • Soybeans (Glycine max), one of major upland crops, require precise management of environmental conditions, such as temperature, water, and soil, during cultivation since they are sensitive to environmental changes. Application of spectral technologies that measure the physiological state of crops remotely has great potential for improving quality and productivity of the soybean by estimating yields, physiological stresses, and diseases. In this study, we developed and validated a soybean growth prediction model using multispectral imagery. We conducted a linear regression analysis between vegetation indices and soybean growth data (fresh weight and LAI) obtained at Miryang fields. The linear regression model was validated at Goesan fields. It was found that the model based on green ratio vegetation index (GRVI) had the greatest performance in prediction of fresh weight at the calibration stage (R2=0.74, RMSE=246 g/m2, RE=34.2%). In the validation stage, RMSE and RE of the model were 392 g/m2 and 32%, respectively. The errors of the model differed by cropping system, For example, RMSE and RE of model in single crop fields were 315 g/m2 and 26%, respectively. On the other hand, the model had greater values of RMSE (381 g/m2) and RE (31%) in double crop fields. As a result of developing models for predicting a fresh weight into two years (2018+2020) with similar accumulated temperature (AT) in three years and a single year (2019) that was different from that AT, the prediction performance of a single year model was better than a two years model. Consequently, compared with those models divided by AT and a three years model, RMSE of a single crop fields were improved by about 29.1%. However, those of double crop fields decreased by about 19.6%. When environmental factors are used along with, spectral data, the reliability of soybean growth prediction can be achieved various environmental conditions.

Improvement of Straw Loading Method on Survival of Mouse IVF/IVC Blastocysts Cryopreserved by Vitrification (체외수정과 체외배양에서 생산된 생쥐 배반포기배의 초자화 동결에 있어서 Straw Loading 방법의 개선)

  • 김선의;엄상준;김은영;윤산현;박세필;임진호
    • Korean Journal of Animal Reproduction
    • /
    • v.20 no.1
    • /
    • pp.35-42
    • /
    • 1996
  • This study was carried out to investigate the effect of straw loading method and thawing protocol on the in vitro development of in vitro produced mouse blastocysts cryopreserved by vitrification. Three loading types of straw I, Il and III on loading and sealing method were made for vitrification. The ability of the solution on straw loading methods to remain vitreous during warming was tested by exposed in air for 1 to 10 s sec. and then plunged the vitrified straws into water bath at 25°C. Embryos to be vitrified were equilibrated to the 20% EG for 5min. and exposed in EFS 40 for 1min. The plug ends of Straw I and Straw II were sealed with straw powder and Straw III was treated straw powder, followed by heat sealing and then plunged into LN$_2$. The results obtained in these experiments were summarized as follows; 1) Straw I embryo column mostly changed from transparent to opaque upon thawing without exposure in air for 3-6 sec. Straw II embryo column was I improved partially but was not remained completely vitreous during warming. However, when Straw lll loading method was used, the embryo column was remained vitreous completely. 2) High survival rates and development rates of each groups (middle blastocysts and hatching blastocysts) of vitrified embryos were obtained by using Straw III loading method than Straw I method (P<0.05). And the range of s standard error was low in Straw lll method. 3) When the embryos vitrified-frozen were placed in air for 3, 5 and l0sec. and then warmed rapidly in water bath at 25$^{\circ}C$, the survival rates after 24h of culture were 72.7-87.1% and the development rates to hatching stage after 48h of culture were 34.0-48.4%. There were no significantly differences according to exposure time in air during warming. In conclusion, the present results showed that highly survival and low standard error of vitrified-frozen mouse bIastocysts were obtained by using straw lll loading, double sealing and appropriate 2 step warming method.

  • PDF

Comparative Functional Analysis of the Malate Dehydrogenase(Mor2) during in vitro Maturation of the Mouse and Porcine Oocytes (체외성숙 과정 중 생쥐와 돼지 난자의 Malate Dehydrogenase(Mor2)의 기능에 대한 비교 분석)

  • Kim, Eun-Young;Kim, Kyeoung-Hwa;Kim, Yun-Sun;Lee, Hyun-Seo;Kim, Yu-Nna;Lee, Kyung-Ah
    • Development and Reproduction
    • /
    • v.11 no.3
    • /
    • pp.263-272
    • /
    • 2007
  • Contrast to mouse where its in vitro maturation rates are high without specific supplements or presence of the cumulus cells, there are some species, such as porcine, where its in vitro oocyte maturation rates are still very low. This comparative study was conducted to investigate the role of malate dehydrogenase(Mor2) during oocyte maturation by RNAi in the mouse and porcine. The Mor2 double-stranded RNA(dsRNA) was prepared speciesspecifically and microinjected into the cytoplasm of denuded germinal vesicle(GV) oocytes. Oocytes were cultured for 48 h(porcine) and 16 h(mouse) in M199 with 10% porcine follicular fluid, pyruvate, p-FSH, EGF, cystein, and estradiol-$17{\beta}$. We measured changes in oocyte morphology, maturation rates and mRNA levels after Mor2 RNAi. We confirmed gene sequence-specific knock down of Mor2 mRNA in both species after Mor2 RNAi. In contrast to our previous finding that mMor2 RNAi resulted in GV arrest in the mouse, we found that pMor2 RNAi resulted in MI arrest in denuded porcine oocytes(58%), but developed to MII(84.4%) in COCs. To determine whether this difference between mouse and porcine RNAi is due to differences in culture media, we cultured mouse oocytes in the M199 media for 16 h after mMor2 RNAi. Mouse oocytes were developed to MII stage(62%) and there was no statistical difference compared to that of non-injected(76.8%) and buffer-injected(73.3%) control groups. Therefore, we concluded that the mouse and porcine oocytes are having different metabolic systems in relation to malate dehydrogenase for oocyte maturation. This could be a basis for differences in maturation rates in vitro in two species. Further scrutinized studies on the metabolic pathways would led us in finding better culture system to improve oocyte maturation rates in vitro, especially in more challenging species like the porcine.

  • PDF

Effects of Amo-1618 on the Yield, Behavior of Mineral Nutritions and Uptake Ratio Employing P-32 Labelled Double Calcium Super-phosphate in Rice Plants (水稻(수도)에 대한 질소(窒素) 및 인산효율증진(燐酸效率增進)에 관한 연구(硏究) -(수도(水稻)에 대한 Amo-1618 처리(處理)가 수량(收量), 무기영양요소(無機營養要素)의 동태(動態) 및 $P^{32}$ 표식중과석(標識重過石)의 이용율(利用率)에 미치는 영향(影響))-)

  • Ahn, Hak-Soo
    • Applied Biological Chemistry
    • /
    • v.11
    • /
    • pp.173-184
    • /
    • 1969
  • To elucidate the effect of Amo-1618(4-hydroxyl-5-isopropyl-2-methlphenyl trimethyl ammonium chloride, 1-piperidine carboxylate) known as a kind of growth retardant, on the growth, grain yield, increasing the efficiency of nitrogen fertilizer, behavior of mineral nutritions and the rate of phosphorus utilization, this experiment was conducted pot culture method in a vinyl house. Two nitrogen level, namely, practical nitrogen level(1 N) and three times nitrogen level(3 N) was made and labelled double-calcium-superphosphate $Ca(H_2P^{32}O_4)_2.\;2H_2O)$ as a source of radioactive phosphorus(P-32) was employed $80\;{\mu}c/pot$, respectively. Rice seedlings, variety 'Suwon No. 82', was transplanted to a 1/50,000 a china pot on June 13 in 1968. For treatment, at early stage of tillering, 10,000 ppm solution of Amo-1618 was foliar sprayed only one time. The Duncan's new mutiple-range test was adopted for statistical analysis evaluating experimental data at 5% level significance. The results obtained may be summarized as follows; 1) No significant difference was found among the treatments in plant height, but in plot of Amo-1618 treatment and 3 N level, number of tillers was significantly increased than that of others. 2) Weight of 10,000 kernels and seed-setting rate was also remarkably increased in same treatment above. 3) Grain yield per pot was significantly increased in Amo-1618 and 3 N level application. This results seemed to be due to the increased the factors on the yield. 4) Contents of nitrogen and phosphorus per cent in the grain was likewise increased in Amo-1618 and 3 N application. There is, however, no difference among treatments in the content of nitrogen and phosphorus in the leaves and culms of rice plants. 5) On the other hand, the contents of potassium and magnesium, no distinctly tendeny showed among treatments. 6) The rate of phosphorus utilization was significantly increased in the plot of Amo-1618 and 3 N application.

  • PDF

Suitability Grouping System of Paddy Soils for Multiple Cropping -Part I: Basic Experiments (다모작(多毛作)을 위한 답토양(畓土壤) 적성등급(適性等級) 구분(區分) -제(第) 1 보(報) : 기초시험(基礎試驗))

  • Jung, Yeun-Tae;Park, Eun-Ho;No, Yeong-Pal;Um, Ki-Tae
    • Korean Journal of Soil Science and Fertilizer
    • /
    • v.19 no.3
    • /
    • pp.211-216
    • /
    • 1986
  • To establish a suitability grouping system of paddy soils for double or multiple cropping with rice which is intensively practiced in the southern parts of Korea, a few basic experiments were carried out for two years. The results are summarized as follows; 1. The potential productivities of the paddy soils which were tested without any fertilizer in the pots of subsoil samples by the double cropping of rice and other upland crops were resulted that the soils of "Moderately well drained" fine silty textured were the highest while the soils of "Poorly drained" sandy were the lowest, and the productivities could be clearly comparable according to the differences of soil conditions. 2. The decomposability of organic matter also was higher in the soils of "Moderately well drained" than the "Imperfectly drained". The coarse loamy and coarse silty textured soils were high in the upland condition and in the early stages of submerging while the fine loamy and fine silty textured soils were high at the late stage of submerging in the rates of organic matter decomposition. 3. The days to be reached to tillable condition after rainfall in fine loamy textured soils were about 5 days earlier than the clayey soils. The period of tillable condition of fine clayey soils with "Moderately well drained" was the longest and that of the fine loamy textured soils was the shortest. But the soils with "Imperfectly drained" were not clear among soil textural classes. 4. The lower the ground water table the higher was the productivity indices. The variation of ground water table in the medium textured soils was higher than the both of coarse and fine textured soils among "Moderately well drained". But it was observed the opposite in the soils of "Imperfectly drained".

  • PDF

Thymocyte Differentiation is Regulated by a Change in Estradiol Levels during the Estrous Cycle in Mouse

  • Lee, Hyojin;Kim, Heejung;Chung, Yiwa;Kim, Jinhee;Yang, Hyunwon
    • Development and Reproduction
    • /
    • v.17 no.4
    • /
    • pp.441-449
    • /
    • 2013
  • Recent study showed that T cells in the immune organs and peripheral blood are influenced by estradiol, leading to a dysfunction of the immune system. However, little is known about the thymic-gonadal relationship during the estrous cycle in mouse. Therefore, the purpose of this study was to elucidate the mechanism by which a change in estradiol levels during the estrous cycle regulates the development of T cells in the mouse thymus. Six-week-old ICR mice were used and divided into four groups, including diestrous, proestrous, estrous, and metestrous. We first confirmed that ER-${\alpha}$ and - ${\beta}$ estrogen receptors were expressed in thymic epithelial cells, showing that their expression was not different during the estrous cycle. There was also no significant difference in thymic weight and total number of thymocytes during the estrous cycle. To determine the degree of thymocyte differentiation during the estrous cycle, we analyzed thymocytes by flow cytometry. As a result, the percentage of CD4+CD8+ double-positive (DP) T cells was significantly decreased in the proestrous phase compared to the diestrous phase. However, CD4+CD8- or CD4-CD8+ (SP) T cells were significantly increased in the proestrous phase compared to the diestrous phase. In addition, the percentage of CD44+CD25- (DN1) T cells was significantly decreased in the estrous phase compared to other phases, whereas the percentages of CD44+CD25+ (DN2), CD44-CD25+ (DN3), and CD44-CD25- (DN4) were not changed during the estrous cycle. These results indicate that the development of thymocytes may arrest in the DP to SP transition stage in the proestrous phase displaying the highest serum level of estradiol. This study suggests that a change in estradiol levels during the estrous cycle may be involved in the regulation of thymocyte differentiation in the mouse thymus.

Nitric oxide-induced immune switching in experimental inflammatory autoimmune diseases

  • Kwak, Hyun-Jeong;Kim, Hyung-Jin;Park, Jae-Sung;Jun, Chang-Duk;Lee, Mun-Young;Shin, Tae-Kyun;Chung, Hun-Taeg
    • IMMUNE NETWORK
    • /
    • v.1 no.2
    • /
    • pp.116-125
    • /
    • 2001
  • Background: Nitric oxide (NO) production has been described as a double-edged sword eliciting both pro- and anti-inflammatory effects in different immune reactions. This work was undertaken to investigate the immunoregulatory role of NO in experimental allergic encephalomyelitis (EAE) and experimental allergic uveitis (EAU). Method: We examined whether molsidomine (MSDM), a NO donor, administration to the myelin basic protein (MBP)- or interphotoreceptor retinoid binding protein (IRBP)-immunized rats could suppress EAE development by shifting toward the Th2 cytokine response. In the EAE experiments, the rats were treated orally with MSDM (10 mg/kg/day) at the early stage (-1~4 days) or throughout the experimental period (-1~15 days). Results: This resulted in significant amelioration of the disease and mild clinical symptoms, while MBP-immunization without MSDM administration showed severe EAE development. A marked reduction in inflammation was also observed in the spinal cord, indicating the crucial role of NO in the pathogenesis of EAE in in vivo. In the EAU experiments, a 24 h pre-treatment with MSDM prior to IRBP immunization resulted in significant inhibition of the disease. Furthermore, MSDM administration for 2 1 days completely reduced the incidence and severity of EAU. To investigate whether MSDM could modulate cytokine switching from Th 1 to Th2, culture supernatants of MBP- or IRBP-stimulated inguinal lymphocytes were analyzed. MSDM treatment enhanced IL-10 secretion but decreased IFN-${\gamma}$. IL-4 was undetectable in all groups. In contrast, the MBP-or IRBP-immunized rats without MSDM secreted high concentrations of IFN-${\gamma}$, but low concentrations of IL-10. Conclusion: In conclusion, NO administation suppresses EAE and EAU by modulating the Th1/Th2 balance during inflammatory immune responses. This work further suggests that NO may be useful in the therapeutic control of autoimmune disease.

  • PDF