• Korean Journal of Microbiology
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• v.21 no.3
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• pp.156-162
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• 1983

Conditions for effcient formation and regeneration of protoplasts of Micromonospora rosaria and Micromonospora purpurea were investigated. The state of inoculm, culture stage and growth in a medium containing partially growth-inhibiting concentration of glycing have significant effects on portoplasting. A high frequency of regeneration (up to 30%) was accomplished with a hypertonic regeneration agar medium defined by Okanishi for Strptomyces. Using the optimal conditions for protroplasting and regeneration, protoplast fusion of auxotrophic M.rosaria was carried out. Polyethylene glycol 1,000 was chosen for fusogenic agent. When signgle auxotrophs were used, the recombinant frequency of auxortrophic markers varied from 1.3 to 3.2%. Using two double auxotrophs, the recombinant frequencies of 0.7-4.3% were obtained. Much lower frequencies(three or more orders of magnitude) were observed by the conventional matings.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.22 no.10
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• pp.934-940
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• 2011

A novel reconfigurable beam steering antenna using double loops is proposed. The double loop antenna has a superposed beam which is produced by combining the in-phase beam in the inner loop with the out-of-phase beam in the outer loop. Also, the doble loop antenna uses two artificial switches to connect between inner loop and outer loop, and has the beam directions of three separate cases(Case 1, Case 2, Case 3) by changing ON/OFF states of switches. The operation frequency of the antenna is 14.5 GHz, and three maximum beam directions of the antenna are ${\phi}_{max}=0^{\circ}$, ${\theta}_{max}=0^{\circ}$(Case 1), ${\phi}_{max}=230^{\circ}$, ${\theta}_{max}=40^{\circ}$(Case 2) and ${\phi}_{max}=130^{\circ}$, ${\theta}_{max}=40^{\circ}$ (Case 3). The peak gains of each case are 6.5 dBi(Case 1), 7.6 dBi(Case 2) and 7.8 dBi(Case 3). The half power beam width(HPBW) of each case is $86{\sim}104^{\circ}$, and the overall HPBW is $160^{\circ}$.

• Transactions of Materials Processing
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• v.27 no.1
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• pp.37-47
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• 2018

In this paper, a double spot welding process, utilizing electric resistance heating dies, is suggested for the spot welding of dissimilar metal plates for drawing and concurrent spot welding. This double welding process has two heating methods for the fusion welding at the interfacial zone between steel and aluminum plates, such as heating method by thermal conduction of electric resistance by welding current induced to heating dies, and heating method by electric resistance between contacted surfaces of two plates by welding current induced to copper electrode. This double welding process has welding variables such as each current induced in heating dies and in copper electrode, outer diameters of heating dies, and edge shape of copper electrode. Experiments for current conditions in welding process should be demanded in order to get successful welding strength. It was known that the welding strength could be reached to the value demanded on industry fields under such welding conditions as heating dies of outer ring dia.12mm contacted on steel plate, as heating dies of outer ring dia. 14mm contacted on aluminum plate, and as copper electrode of dia. 6.0mm, and as 3 times continuous heating method by $1^{st}$ current of 11 kA(9cycle), $2^{nd}$ current 11 kA(60cycle), $3^{rd}$ current 7 kA(60cycle) applied in steel heating dies and copper electrodes, flat edge of copper electrode, for double spot welding process of dissimilar metal plates of steel and aluminum of 1.0 mm thickness.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.3 no.5
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• pp.461-474
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• 2009

The cognitive radio (CR) technique is a useful tool for improving spectrum utilization by detecting and using the vacant spectrum bands in which cooperative spectrum sensing is a key element, while avoiding interfering with the primary user. In this paper, we propose a novel cluster-based cooperative spectrum sensing scheme in cognitive radio with two solutions for the purpose of improving in sensing performance. First, for the cluster header, we use the double adaptive energy thresholds and a multi-bit quantization with different quantization interval for improving the cluster performance. Second, in the common receiver, the weighed HALF-voting rule will be applied to achieve a better combination of all cluster decisions into a global decision.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.4 s.175
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• pp.1039-1046
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• 2000

This paper introduces the control and measurement of a double parallel robot manipulator applied for unknown geometric surface grinding. A measurement system is developed to recognize a grinding path by a vision camera and to observe a grinding load by a current sensor. With the measured fusion information, an intelligent controller identifies the unknown geometric surface and moves the robot along the grinding path with a constant grinding load.

• Journal of Environmental Science International
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• v.26 no.1
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• pp.11-21
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• 2017

In this study, bacterial growth was assessed by flow cytometry analysis of fluorescent probes-stained bacteria. Flow cytometry has many advantages of rapid analytical time, a low standard deviation, and highly sensitive detection of live and Dead E.coli over colony forming assay. When untreated bacteria were stained by using Thiazole Orange (TO) and Propidium Iodide (PI), double staining had a short analytical time as compared with that of single staining while its error rate was similar to that of single staining. Through double staining experiments, it was determined that optimal concentrations for TO and PI staining were 420 nM and $9.6{\mu}M$, respectively.

• Proceedings of the KIEE Conference
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• 1993.07b
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• pp.591-593
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• 1993

Magneto plasma has been widely used for controlled fusion and material processings such as etchings, depositions, and sputterings in the various fields. For controlling and guaranting the equipment, precision plasma parameters must be gained. In this experiment, using single probe and double probes, we investigated the effect of magnetic field on magneto plasma. As a result of this experiment, we found the fact that single probe was largely affected by magnetic field for measuring magneto plasma parameters, while double probe was not.

• Journal of Multimedia Information System
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• v.8 no.1
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• pp.23-30
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• 2021

Palmprint has become a popular biometric modality; however, palmprint recognition has not been conducted in video media. Video palmprint recognition (VPR) has some advantages that are absent in image palmprint recognition. In VPR, the registration and recognition can be automatically implemented without users' manual manipulation. A good-quality image can be selected from the video frames or generated from the fusion of multiple video frames. VPR in contactless mode overcomes several problems caused by contact mode; however, contactless mode, especially mobile mode, encounters with several revere challenges. Double-line-single-point (DLSP) assisted placement technique can overcome the challenges as well as effectively reduce the localization error and computation complexity. This paper modifies DLSP technique to reduce the invalid area in the frames. In addition, the valid frames, in which users place their hands correctly, are selected according to finger gap judgement, and then some key frames, which have good quality, are selected from the valid frames as the gallery samples that are matched with the query samples for authentication decision. The VPR algorithm is conducted on the system designed and developed on mobile device.

• Korean Chemical Engineering Research
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• v.53 no.6
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• pp.672-676
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• 2015

Spherical phospholipid bilayers, vesicles, were formed with respect to phase of each layer via a double emulsion technique. The conversion of phosphatidylcholine (PC) to phosphatidic acid (PA) at the outer layer, caused by phospholipase D (PLD), induced a curvature change in the vesicles, which eventually led them to fuse each other. The effect of the lipid layer physical-properties on the PLD-induced vesicle fusion was investigated using the fluorescence intensity change. 8-Aminonaphthalene-1,3,6-trisulfonic acid disodium salt(ANTS) and p-Xylene-bis(N-pyridinium bromide)(DPX) were encapsulated in the vesicles, respectively, for the quantification of the fusion. The fluorescence scale was calibrated with the fluorescence of a 1/1 mixture of ANTS and DPX vesicles in NaCl buffer taken as 100% fluorescence (0% fusion) and the vesicles containing both ANTS and DPX as 0% fluorescence (100% fusion), considering the leakage into the medium studied directly in a separate experiment using vesicles containing both ANTS and DPX. It was observed that the fusion occurred to the liquid-phase of the inner layer only. The fusion behaviors were very similar for both solid and liquid of the outer layer. However, the leakage was faster for the solid-phase outer-layer than the liquid-phase outer-layer. The difference in the leakage seems to be caused by the lipid concentration and the lateral diffusivity in the layer.

• Journal of Korean Neurosurgical Society
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• v.56 no.2
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• pp.103-107
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• 2014

Objective : This study analyzed clinical and radiological outcomes of a zero-profile anchored spacer (Zero-P) and conventional cage-plate (CCP) for single level anterior cervical discectomy and fusion (ACDF) to compare the incidence and difference of postoperative dysphagia with both devices. Methods : We retrospectively reviewed our experiences of single level ACDF with the CCP and Zero-P. From January 2011 to December 2013, 48 patients who had single level herniated intervertebral disc were operated on using ACDF, with CCP in 27 patients and Zero-P in 21 patients. Patients who received more than double-level ACDF or combined circumferential fusion were excluded. Age, operation time, estimated blood loss (EBL), pre-operative modified Japanese Orthopaedic Association (mJOA) scores, post-operative mJOA scores, achieved mJOA scores and recovery rate of mJOA scores were assessed. Prevertebral soft tissue thickness and postoperative dysphagia were analyzed on the day of surgery, and 2 weeks and 6 months postoperatively. Results : The Zero-P group showed same or favorable clinical and radiological outcomes compared with the CCP group. Postoperative dysphagia was significantly low in the Zero-P group. Conclusions : Application of Zero-P may achieve favorable outcomes and reduce postoperative dysphagia in single level ACDF.