• Korean Journal of Clinical Pharmacy
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• v.7 no.2
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• pp.86-90
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• 1997

This study was to find a more accurate method fur measuring small vancomycin dosages which are commonly used in neonates by comparing single and double dilution method. For single dilution method, 500 mg of vancomycin powder was accurately measured and reconstituted with 5 ml of distilled water to make a concentration of 100 mg/ml. Volumes of 0.05, 0.1, 0.15, 0.2, 0.3, 0.4, and 0.5 ml, which equal the target dosages of 5, 10, 15, 20, 30, 40, and 50 mg, were measured using syringes made by Shina and each sample was further diluted with 2 ml of $5\%$ dextrose. The solution of 100 mg/ml concentration was further diluted with $5\%$ dextrose to make a concentration of 20 mg/ml. Volumes of 0.25, 0.5, 0.75, 1.0, 1.5, 2.0, and 2.5 ml, which correspond to 5, 10, 15, 20, 30, 40, and 50 mg, were sampled by the same Shina's syringe as in single dilution method and then each sample was further diluted to make a total volume of 10 ml. Each sample was analyzed by HPLC. The measured dosages of each sample in both single and double dilution methods were lower than the target dosages; however, e values in double dilution method were higher than those in single dilution method for seven target dosages. Percent target dosages in single dilution method were 65 to $90\%$, while in double dilution method 91 to $94\%$. Statistically significant difference between two groups was shown in 5, 10, 15, 20, and 40 mg dosages (p<0.05). In conclusion, when preparing small vancomycin dosages lower an 20 mg $(volume{\leq}0.2\;ml)$, using Shina's syringes, the double dilution method has a closer value to the target dosage than single dilution method.

• Journal of the Korean Chemical Society
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• v.28 no.5
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• pp.302-308
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• 1984

X-ray spectrometric determination of $Ta_2O_54, $Nb_2O_5$ and $ZrO_2$ in tin-slag samples using the double dilution technique was conducted. Tin-slag samples and one synthesized standard sample having similar composition to the samples were diluted with anhydrous $Li_2B_4O_7$ at the level of 1.00, 2.00 and 3.00% of the sample content, respectively. The diluted samples were fused at $1,150^{\circ}C$ for 30 minutes, and these glass beads were finely ground and pelletized. With the X-ray intensities measured, analytical results were calculated by the double dilution equation. The results agreed to the reference values obtained by the standard calibration method and the simple dilution technique within allowable error range and were reproducible.

• Analytical Science and Technology
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• v.16 no.1
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• pp.82-89
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• 2003

Isotope dilution mass spectrometry (IDMS) was applied to the determination of Ni, Cr, Mo in low alloy steel reference materials. The Mo isotope ratio measurement was performed by dynamic reaction cell inductively coupled plasma mass spectrometry (DRC-ICP/MS) using ammonia as a reaction cell gas. In the case of Ni and Cr measurement, all data were obtained at medium resolution mode (m/${\Delta}m=3000$) of double focusing sector field high resolution inductively coupled plasma mass spectrometry (HR-ICP/MS). For the method validation of the technique was assessed using the certified reference materials such as NIST SRM 361, NIST SRM 362, NIST SRM 363, NIST SRM 364, NIST SRM 36b. This method was applied to the determination of Ni, Cr and Mo in low alloy steel sample (CCQM-P25) provided by NMIJ for international comparison study.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.27 no.8
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• pp.440-447
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• 2015

In the shipbuilding process, the confined work spaces of the ship are formed continuously; the ventilation method can therefore be limited to dilution ventilation due to the complex structure and limited opening of the confined space. Also, it is difficult to evaluate air-quality using measurement and an adequate ventilation method. CFD simulation methods are typically used in analyzing ventilation efficiency in these cases. In this study, a method is suggested to analyze the air quality of the complex enclosures in shipbuilding using CFD. Especially, among the conventional indices, the ventilation efficiency scales or indices, the supply air contribution (SVE4), and the age of the air (SVE3) (fit for the confined and complex enclosure) were applied.

• Food Science of Animal Resources
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• v.24 no.2
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• pp.182-188
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• 2004

To determine the concentration of Lactoperoxidase (LPO), an indirect enzyme-linked immunosorbant assay(ELISA) was developed. Anti-LPO egg yolk immunoglobulin(IgY) was transferred to egg yolk by immunizing of Brown hens with LPO. The titer of purified anti-LPO IgY was 1: 520,000. The immunological response of anti- LPO IgY with ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin, casein and lysozyme were evaluated, resulting that the anti-LPO IgY found to be a specific antibody toward LPO and no cross-reaction was observed against ${\alpha}$-lactalbumin, ${\beta}$-lactoglobulin, casein, and lysozyme in double immunodiffusion test and ELISA test. In indirect ELISA method, coating concentration of LPO and dilution rate of anti-LPO IgY was 0.25$\mu\textrm{g}$/mL and 1:8,000 respectively. Sensitivity in the standard curve of LPO was ranged from 0.01 to 1$\mu\textrm{g}$/mL using anti-LPO IgY.

• Journal of Korean Society for Atmospheric Environment
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• v.10 no.E
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• pp.325-331
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• 1994

Organic extracts of diesel- exhaust particulates were analyzed for mutagenicity using Ames Salmonella typhimurium assay system. An experimental diesel microbus used was drived on the chassis dynamometer according to CVS-75 mode. The samples were taken from the mixed gases in a dilution tunnel. With a high-volume air sampler equipped with double filter holders, particulate matters were collected on a teflon-coated glass fiber filter placed behind a activated carbon filter. After ultrasonic extraction with benzene- ethanol and evaporation to dryness, the residue was dissolved in dimethylsulfoxide. Each sample was tested toward 2 strains, TA100 and TA98 by the pre-incubation method in the absence and presence of S-9mix. Average concentration of diesel- exhaust particulates was about 116.6mg/m$^3$, and 44.1~62.2 ％ to the total weight of particulates, consisted of organic matters. The mutagenicities of diesel- exhaust particulate organic matters were 4,512 and 2,205 revertants/m$^3$ toward TA100 without and with S-9mix, respectively. Those toward TA98 were 13,367 and 3,715 revertants/m$^3$, respectively.

• Elastomers and Composites
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• v.51 no.1
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• pp.43-48
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• 2016

Multi-Surface (MS) treatment is a new technique of surface treatment to reduce the static friction factor on the surface of rubber. MS treatments include 4 methods which names are MS-V (UV-irradiation on the rubber surface), MS-M (doing the chemical reaction with double bond of rubber), MS-Q (dilution of rubber surface by silicone surfactant), and MS-P (coating and heating of rubber surface). The experiment and test of every MS-treatment had been carried out using acrylonitrile-butadiene rubber (NBR), ethylene-propylene-diene rubber (EPDM), and chlorosulphonated rubber (CSM) as rubber materials. It had introduced the steps of every MS-treatment process and the result of the properties test. From the research, it was found that the best method was MS-V treatment because it suited all the samples and the effect was obviously.

• Journal of the Korea Convergence Society
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• v.9 no.3
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• pp.217-222
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• 2018

In this paper, a new micro polymer chip platform and protocol were developed for rare cell sample preparation. The proposed platform and protocol overcome the current limitation of the dilution method which is based on statistics and the FACS method which expensive and requires fluorescence staining. It allows collecting exact number of target cells simply and selectively because the cells are visually confirmed during the collecting process. The collected cells can be transported or spiked into a desired locations, such as a microchamber, without cell loss. This research may applicable not only to a rare cell sample preparation for Lab on a Chip cancer diagnosis, but also to a single/double/multiple cell sample preparation for a cell analysis field. To verify this platform and protocol, five human breast cancer cells (MCF-7) were collected and transported into a hemocytometer chamber.

• Microbiology and Biotechnology Letters
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• v.34 no.4
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• pp.342-351
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• 2006

The aim of this study was to survey susceptibilities of Escherichia coli and Klebsiella pneumoniae isolates against cefotaxime and to determine the prevalences of CTX-M type extended-spectrum $\beta$-lactamases (ESBLs) producing E. coli and K. pneumoniae in Korea. During the period of February to July, 2005, 153 E. coli and 52 K. neumoniae isolates were collected from 2 hospitals in Busan. Antimicrobial susceptibilities to cefotaxime were tested by the disk diffusion method. ESBL production of E. coli and K. pneumoniae was determined by the double disk synergy test. MICs of $\beta$-lactam antibiotics were determined by the agar dilution method. Blac$_{CTX-M}$ genes of the organism were detected by PCR. Among 153 isolates of E. coli and 52 isolates of K. neumoniae, 27 (17.6%) and 25 (48.0%) were intermediate or resistant to cefotaxime, respectively. Twenty-three (15.0%) isolates out of 153 E. coli and 13 (25.0%) out of 52 K. neumoniae isolates showed positive results for ESBL by the double disk synergy test. Twenty isolates out of 23 ESBL producing E. coli and 12 out of 13 ESBL producing K. neumoniae isolates harbored biacTx-M gene,11 of ESBL producing E. coli and 12 of ESBL producing K. neuinoniae isolates harbored bla$_{CTX-M}$ gene, 11 of the ESBL producing E. coli and 2 of ESBL producing K. neumoniae isolates harbored bla$_{TEM}$ gene, and 1 of the ESBL producing E. coli and 12 of ESBL producing K. neumoniae isolates harbored bla$_{SHV}$ gene. E. coli and K. neumoniae isolates producing CTX-M-type ESBLs were not uncommon in Korea. It is thought that continuous survey are necessary for inspecting the spread and novel variants of CTX-M-type ESBL genes. Further me]'e investigation and research on ESBL producing strains are needed in order to prevent the spread of resistant bacteria.

• Bulletin of the Korean Chemical Society
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• v.34 no.12
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• pp.3817-3824
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• 2013

Various separation modes in HPLC, such as anion exchange (AE), reversed-phase (RP), and affinity (AF) chromatography were examined for the separation of selenium species in human blood serum and urine. While RP- and AE-HPLC were mainly used for the separation of small molecular selenium species, double column AF-HPLC achieved the separation of selenoproteins in blood serum efficiently. Further, the effluent of AF-HPLC was enzymatically hydrolyzed and then analyzed with RP HPLC for selenoamino acid study. The versatility of the hybrid technique makes the in-depth study of selenium species possible. For quantification, post column isotope dilution (ID) with $^{78}Se$ spike was performed. ORC ICP/MS (octapole reaction cell inductively coupled plasma/mass spectrometry) was used with 4 mL $min^{-1}$ Hydrogen as reaction gas. In urine sample, inorganic selenium and SeCys were identified. In blood serum, selenoproteins GPx, SelP and SeAlb were detected and quantified. The concentration for GPx, SelP and SeAlb was $22.8{\pm}3.4\;ng\;g^{-1}$, $45.2{\pm}1.7\;ng\;g^{-1}$, and $16.1{\pm}2.2\;ng\;g^{-1}$, respectively when $^{80}Se/^{78}Se$ was used. The sum of these selenoproteins ($84.1{\pm}4.4\;ng\;g^{-1}$) agrees well with the total selenium concentration measured with the ID method of $87.0{\pm}3.0\;ng\;g^{-1}$. Enzymatic hydrolysis of each selenium proteins revealed that SeCys is the major amino acid for all three proteins and SeMet is contained in SeAlb only.