• 한국정밀공학회지
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• 제21권9호
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• pp.48-55
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• 2004

This study presents a genetic algorithm-based method fur optimizing control parameters in the pressure control of electro-hydraulic pump with variable displacement. Genetic algorithms are general-purpose optimization methods based on natural evolution and genetics and search the optimal control parameters maximizing a measure that evaluates the performance of a system. Four control gains of the PI-PD cascade controller for an electro-hydraulic pressure control system are optimized using a genetic algorithm in the experiment. Optimized gains are confirmed by inspecting the fitness distribution which represents system performance in gain spaces. It is shown that genetic algorithm is an efficient scheme in optimizing control parameters of the pressure control of electro-hydraulic pump with variable displacement.

• 한국항공운항학회지
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• 제18권1호
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• pp.33-38
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• 2010

Developments of numerical methods are very important to design and analysis for a high subsonic turbine blade. In general, Analysis by experimental investigation has needed a lot of human resources and required time, indispensably, and equipments still have a limit to measure in conditions of high temperature. Rapid technological developments of CPU and integration level of memory make it possible to advance computation with almost exactly simulation so, recent developments of numerical methods are in spotlight. In the present study, the panel method, which is well-known as relatively simplified numerical method, and 2-dimensional ordinary differential Falkner-Skan equation were computed in order to analyze the outer flow, and FVM-based solid heat transfer equation, was also computed to forecast the temperature distribution of the airfoil and the turbine blade. Unstructured grid was constructed in the turbine blade, which has double cooling holes, in order to analyze the internal heat transfer. Cooling fluid was assumed as fully-developed turbulent flow and that circulated in cooling holes.

• International Journal of Fluid Machinery and Systems
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• 제2권2호
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• pp.127-135
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• 2009

A conventional centrifugal pump causes a drastic deterioration of air-water two-phase flow performances even at an air-water two-phase flow condition of inlet void fraction less than 10% in the range of relatively low water flow rate. Then we have developed a two-phase flow centrifugal pump which consists of a tandem arrangement of double rotating cascades and blades of outer cascade have higher outlet angle more than $90^{\circ}$. In design of the two-phase flow pump for various sized and operating conditions, similarity relations of geometric dimensions to hydraulic performances is very useful. The similarity relations of rotational speed, impeller diameter and blade height are investigated for the developed impeller in the present paper. As the results, the similarity law of rotational speed and impeller diameter is clarified experimentally even in two-phase flow condition. In addition, influences of blade height on air-water two-phase flow performances indicate a little difference from the similarity relations.

• Journal of Ginseng Research
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• 제41권2호
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• pp.227-231
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• 2017

Background: Ginsenosides are active components of Panax ginseng that exert various health benefits including kidney protection effect. The medicinal activity of ginsenosides can be enhanced by modulating their stereospecificity by heat processing. Ginsenosides Rk2 and Rh3 represent positional isomers of the double bond at C-20(21) or C-20(22). Methods: The present study investigated the kidney-protective effects of ginsenosides Rk2 and Rh3 against cisplatin, a platinum based anticancer drug, induced apoptotic damage in renal proximal LLC-PK1 cells. Results: As a result, ginsenoside Rh3 shows a stronger protective effect than that shown by Rk2. Cisplatin-induced elevated protein levels of phosphorylated c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), p38, and cleaved caspase-3 decreased after cotreatment with ginsenoside Rh3. The increase in the percentage of apoptotic LLC-PK1 cells induced by cisplatin treatment also significantly reduced after cotreatment with ginsenoside Rh3. Conclusion: These results demonstrate that inhibition of the JNK and ERK mitogen-activated protein kinase signaling cascade plays a critical role in mediating the renoprotective effect of ginsenoside Rh3.

• Nuclear Medicine and Molecular Imaging
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• 제40권2호
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• pp.113-119
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• 2006

Coronary artery disease is a loading cause of morbidity and mortality across the world. Percutaneous coronary intervention has become the major technique of revascularization. However, restenosis remains a major limitation of this procedure. Recently the need for repeat intervention due to restenosis, the most vexing long-term failure of percutaneous coronary intervention, has been significantly reduced owing to the introduction of two major advances, intracoronary brachytherapy and the drug-eluting stents. Intracoronary brachytherapy has been employed in recent years to prevent restenosis lesions with effective results, principally in in-stent restenosis. Restenosis is generally considered as au excessive form of normal wound healing divided up in precesses: elastic recoil, neointimal hyperplasia, and negative vascular remodeling. Restenosis has previously been regarded as a proliferative process in which neointimal thickening, mediated by a cascade of inflammatory mediators and other factors, is the key factor. Ionizing radiation has been shown to decrease the proliferative response to injury in animal models of restenosis. Subsequently, several randomized, double blind trials have demonstrated that intracoronary brachytherapy can reduce the rates of both angiographic restenosis and clinical event rates in patients undergoing percutaneous coronary intervention for in stent restenosis. Some problems, such as late thrombosis and edge restenosis, have been identified as limiting factors of this technique. Brachytherapy is a promising method of preventing and treating coronary artery restenosis.

• 디지털융복합연구
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• 제19권2호
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• pp.245-251
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• 2021

1,2인 가구가 꾸준하게 늘어나고 있는 추세에 비어 있는 시간대에 집을 방문하는 외부인이 누구인지 확인하고 싶은 요구가 증가하고 있다. 얼굴인식 기술은 많은 연구를 통해 여러 가지 모델이 제안되었는데 OpenCV의 Harr Cascade와 Dlib의 Hog가 대표적인 오픈소스 모델이다. 두 모델은 사용 환경에 따른 장단점을 가지고 있는데, 본 연구에서 초점을 둔 실내 현관 앞과 제한된 거리에서는 Dlib의 Hog가 강점을 가진다. 본 논문에서는 딥러닝 오픈 소스인 Dlib에 기반을 둔 얼굴인식 방문자 출입관리 시스템을 설계하고 구현하였다. 전체 시스템은 프론트 모듈과 서버모듈, 모바일모듈로 구성되며 세부적으로는 얼굴등록, 얼굴인식, 실시간 방문자 확인 및 원격제어, 동영상 저장 기능을 포함한다. 인터넷에서 공개된 사진을 이용하여 거리임계 값의 변화에 따른 정밀도, 특이도, 정확도를 구하고 선행연구 결과와 비교하였다. 실험 결과 구현된 시스템이 정상적으로 동작하는 것을 확인 하였으며 Dlib에서 보고한 것과 비슷한 결과를 보이는 것을 확인 하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권18호
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• pp.8177-8186
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• 2016

The present study was aimed at determining the effects of alkylating and oxidative stress inducing agents on a newly identified variant of DNA polymerase beta ($pol{\beta}{\Delta}_{208-304}$) specific for ovarian cancer. $Pol{\beta}{\Delta}_{208-304}$ has a deletion of exons 11-13 which lie in the catalytic part of enzyme. We compared the effect of these chemicals on HeLa cells and HeLa cells stably transfected with this variant cloned into in pcDNAI/neo vector by MTT, colony forming and apoptosis assays. $Pol{\beta}{\Delta}_{208-304}$ cells exhibited greater sensitivity to an alkylating agent and less sensitivity towards $H_2O_2$ and UV when compared with HeLa cells alone. It has been shown that cell death in $Pol{\beta}{\Delta}_{208-304}$ transfected HeLa cells is mediated by the caspase 9 cascade. Exon 11 has nucleotidyl selection activity, while exons 12 and 13 have dNTP selection activity. Hence deletion of this part may affect polymerizing activity although single strand binding and double strand binding activity may remain same. The lack of this part may adversely affect catalytic activity of DNA polymerase beta so that the variant may act as a dominant negative mutant. This would represent clinical significance if translated into a clinical setting because resistance to radiation or chemotherapy during the relapse of the disease could be potentially overcome by this approach.

• Journal of Radiation Protection and Research
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• 제47권1호
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• pp.50-57
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• 2022

Background: There are several proton therapy facilities in operation or planned in Taiwan, and these facilities are anticipated to not only treat cancer but also provide beam services to the industry or academia. The simplified approach based on the Monte Carlo-based data sets (source terms and attenuation lengths) with the point-source line-of-sight approximation is friendly in the design stage of the proton therapy facilities because it is intuitive and easy to use. The purpose of this study is to expand the Monte Carlo-based data sets to allow the simplified approach to cover the application of proton beams more widely. Materials and Methods: In this work, the MCNP6 Monte Carlo code was used in three simulations to achieve the purpose, including the neutron yield calculation, Monte Carlo-based data sets generation, and dose assessment in simple cases to demonstrate the effectiveness of the generated data sets. Results and Discussion: The consistent comparison of the simplified approach and Monte Carlo simulation results show the effectiveness and advantage of applying the data set to a quick shielding design and conservative dose assessment for proton therapy facilities. Conclusion: This study has expanded the existing Monte Carlo-based data set to allow the simplified approach method to be used for dose assessment or shielding design for beam services in proton therapy facilities. It should be noted that the default model of the MCNP6 is no longer the Bertini model but the CEM (cascade-exciton model), therefore, the results of the simplified approach will be more conservative when it was used to do the double confirmation of the final shielding design.

• 대한본초학회지
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• 제27권6호
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• pp.131-137
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• 2012

Objectives : Cyclooxygenase (COX) plays a central role in the inflammatory cascade by converting arachidonic acid into prostaglandin. COX-2 is typically induced by inflammatory stimuli in the majority of tissues, it is responsible for propagating the inflammatory response and thus, considered as the best target for anti-inflammatory drugs. The present study investigated the modulatory effect of ginsenoside Rg3, a principle active ingredient in Panax ginseng, on COX-2 expression in the brain tissue induced by systemic lipopolysaccharide (LPS) treatment in C57BL/6 mice. Methods : Because systemic LPS treatment induces COX-2 expression immediately in the brain, ginsenoside Rg3 was treated orally with doses of 10, 20, and 30 mg/kg at 1 hour before the LPS (3 mg/kg, i.p.) injection. At 4 hours after the LPS injection, COX-2 mRNA was measured by real-time polymerase chain reaction method, COX-2 protein levels were measured by Western blotting. In addition, COX-2 expressions in brain tissue were observed with immunohistochemistry and double immunofluoresence labeling. Results : Ginsenoside Rg3 (20 and 30 mg/kg) significantly attenuates up-regulation of COX-2 mRNA and protein expression in brain tissue at 4 hours after the LPS injection. Moreover, ginsenoside Rg3 (20 mg/kg) significantly reduced the number of COX-2 positive neurons in the cerebral cortex and amygdala. Conclusion : These results indicate that ginsenoside Rg3 plays a modulatory role in neuroinflammation through the inhibition of COX-2 expression in the brain and suggest that ginsenoside Rg3 and ginseng may be effective on neurodegenerative diseases caused by neuroinflammation.

• IMMUNE NETWORK
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• 제20권5호
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• pp.43.1-43.11
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• 2020

Capicua (CIC) is a transcriptional repressor that regulates several developmental processes. CIC deficiency results in lymphoproliferative autoimmunity accompanied by expansion of CD44^hiCD62L^lo effector/memory and follicular Th cell populations. Deletion of Cic alleles in hematopoietic stem cells (Vav1-Cre-mediated knockout of Cic) causes more severe autoimmunity than that caused by the knockout of Cic in CD4⁺CD8⁺ double positive thymocytes (Cd4-Cre-mediated knockout of Cic). In this study, we compared splenic CD4⁺ T cell activation and proliferation between whole immune cell-specific Cic-null (Cic^f/f;Vav1-Cre) and T cell-specific Cic-null (Cic^f/f;Cd4-Cre) mice. Hyperactivation and hyperproliferation of CD4⁺ T cells were more apparent in Cic^f/f;Vav1-Cre mice than in Cic^f/f;Cd4-Cre mice. Cic^f/f;Vav1-Cre CD4⁺ T cells more rapidly proliferated and secreted larger amounts of IL-2 upon TCR stimulation than did Cic^f/f;Cd4-Cre CD4⁺ T cells, while the TCR stimulation-induced activation of the TCR signaling cascade and calcium flux were comparable between them. Mixed wild-type and Cic^f/f;Vav1-Cre bone marrow chimeras also exhibited more apparent hyperactivation and hyperproliferation of Cic-deficient CD4⁺ T cells than did mixed wild-type and Cic^f/f;Cd4-Cre bone marrow chimeras. Taken together, our data demonstrate that CIC deficiency at the beginning of T cell development endows peripheral CD4⁺ T cells with enhanced T cell activation and proliferative capability.