• Title/Summary/Keyword: Dose report

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Aphicidal Activity of Starfish (Asterina pectinifera) Extracts against Green Peach Aphid (Myzus Persicae) (불가사리 추출물의 복숭아혹진딧물(Myzus Persicae) 살충활성)

  • Jang, Ja-Yeong;Yi, Sol;Jung, Iee-Young;Choi, Eun-Hyun;Jo, Uk-Hee;Seo, Jung-Mi;Yang, Si-Young;Kim, In-Seon
    • Korean Journal of Environmental Agriculture
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    • v.30 no.2
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    • pp.173-178
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    • 2011
  • BACKGROUND: Starfish is one of major sea invertebrates that have become a serious economic threat to aquacultural farms in Korea. Much effort has sacrificed to reduce the economic losses of the farms by predatory starfish, including developing and searching biological resources for medicinal and agricultural purposes. In the present study, we investigated aphicidal activity of the extracts from the starfish Asterina pectinifera against green peach aphid. METHODS AND RESULTS: Fresh starfishes were cut into small pieces, homogenized and soaked in methanol. The methanol extracts were centrifuged and the resulting supernatant was subjected to aphicidal activity assays and a series of silica gel column chromatography. More than 70% mortality of aphids were observed by the extracts at a concentration of 1,000 mg/L, exhibiting dose-dependent mortality. TOF-MS analyses detected polyhydroxysteroid as a main aphicidal compound from the starfish extracts. Transmission electronic microscopy could demonstrate that the extracts with polyhydroxysteroid caused aphids death by affecting their epicuticular membrane. CONCLUSION(s): This is the first report of aphicidal activity of the starfish Asterina pectinifera extracts against green peach aphid. Starfish biological resources may be used as a potential candidate for developing a new type natural insecticide.

Conformal Radiotherapy in a Patient with Cancer at the base of the Tongue in a Previously Irradiated Area (방사선치료 조사영역 내에 발생한 설암 환자에서 입체조형방사선치료 경험 : 증례보고)

  • Cho Moon-June;Kim Ki-Hwan;Kim Byung-Kook;Song Chang-Joon;Kim Jun-Sang;Kim Jae-Sung;Jang Ji-Young
    • Korean Journal of Head & Neck Oncology
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    • v.17 no.1
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    • pp.59-62
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    • 2001
  • Objectives: We report an interim result of conformal radiotherapy in a patient with early stage cancer at the base of the tongue, which developed in a previously irradiated area. Materials and Methods: A 64-year-old male patient was diagnosed with T4N0M0 supraglottic cancer. He received 72Gy of radiation therapy from 21 November 1988 to 24 February 1989. He had local failure and underwent a salvage total laryngectomy on 28 August 1989. Subsequently, he did well. In early 1999, he suffered from throat pain. He had a 2.5cm ulcerative mass at the base of his tongue, in the area that had been irradiated previously. Biopsy showed squamous cell carcinoma. After workup, he was diagnosed with base of tongue cancer with T2N0M0. Surgery was not feasible because the morbidity was not acceptable. Since it was difficult to re-irradiate the area with a curable dose using conventional 2D radiation therapy with an acceptable morbidity, we decided to try conformal radiotherapy. We used 7 static beam ports with field sizes from $7x6.4\;to\;8x8cm^2$, using 6 and 10MV photons. The fractionation regimen was 1.8Gy, 5 times per week. He received 64.8Gy in 36 fractions from 9 April 1999 to 1 June 1999. Results: In the 21 months since radiotherapy, the patient has not experienced any acute or chronic complications, such as xerostomia. He experienced relief of pain shortly after the start of radiotherapy, showed a complete response, and is still doing well. Conclusion: Conformal radiotherapy can be used to treat cancer that develops within a previously irradiated field, with curative intent.

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Optimal Cutoff Points of Rate Pressure Product in Each Stage of Treadmill Exercise Test According to the Degree of Metabolic Syndrome in Korean Adults (한국성인의 대사증후군 예방을 위한 운동부하 검사시 각 단계별 심근부담률의 적정 임계점)

  • Shin, Kyung-A
    • Korean Journal of Clinical Laboratory Science
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    • v.50 no.2
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    • pp.136-143
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    • 2018
  • The rate pressure product (RPP) is expressed as a product of the heart rate and systolic blood pressure as an index indirectly measuring the myocardial oxygen consumption, and it indicates the burden on the myocardium. The aim of this study was to determine the optimal level of RPP for preventing metabolic syndrome in a treadmill exercise test in Korean adults. Metabolic syndrome was the diagnosis of the third executive summary report on the National Cholesterol Education Program Adult Treatment Panel III (NCEP-ATP III) criteria. According to the criteria, the metabolic syndrome diagnosis group (MetS, N=25), pre-metabolic syndrome group (Pre-MetS, N=106), and non-risk factor group (Non-MetS, N=65) were classified. The exercise stress test was performed based on the Bruce protocol. The RPP was calculated as (heart rate${\times}$systolic blood pressure)${\div}1,000$. The results showed that the maximum systolic blood pressure was high despite the low daily dose reached in the diagnostic group of metabolic syndrome. The optimal threshold of the RPP at the time of the exercise treadmill test for a metabolic syndrome prediction was $12.56mmHg{\times}beats/min{\times}10^{-3}$ in the first stage of the exercise stress test. The second stage of the exercise test was $16.94mmHg{\times}beats/min{\times}10^{-3}$, and at the third stage of the exercise test was $21.11mmHg{\times}beats/min{\times}10^{-3}$.

Echinostoma aegyptica (Trematoda: Echinostomatidae) Infection in Five Riparian People in Savannakhet Province, Lao PDR

  • Chai, Jong-Yil;Jung, Bong-Kwang;Chang, Taehee;Shin, Hyejoo;Sohn, Woon-Mok;Eom, Keeseon S.;Yong, Tai-Soon;Min, Duk-Young;Phammasack, Bounlay;Insisiengmay, Bounnaloth;Rim, Han-Jong
    • Parasites, Hosts and Diseases
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    • v.58 no.1
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    • pp.67-72
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    • 2020
  • Human infection with Echinostoma aegyptica Khalil and Abaza, 1924 (Trematoda: Echinostomatidae) is extremely rare. In this study, we confirmed E. aegyptica infection in 5 riparian residents living along the Mekong River in Savannakhet Province, Lao PDR. The patients revealed eggs of Opisthorchis viverrini/minute intestinal flukes, echinostomes, and other parasites in fecal examinations using the Kato-Katz technique. Following treatment with praziquantel 30-40 mg/kg and pyrantel pamoate 10-15 mg/kg in a single dose and purging with magnesium salts, adult specimens of various helminth species were collected. Among the trematodes, echinostome flukes of 4.5-7.6 mm in length (n = 134; av. 22.3 specimens per case) were of taxonomic interest and subjected in this study. The flukes were morphologically characterized by having total 43-45 collar spines arranged in 2 alternating rows (corner spines usually 5 on each side) and compatible with previous descriptions of E. aegyptica. The patients were mixed-infected with other helminths, so specific clinical manifestations due to this echinostome fluke were difficult to determine. The present paper describes for the first time human E. aegyptica infections in Lao PDR. This is the second report of human infection (2nd-6th cases) with E. aegyptica in the world following the first one from China.

Inhibitory Effects of Syzygium aromaticum Ethanol Extracts on IgE Mediated RBL-2H3 cell Activation (IgE 매개 RBL-2H3 세포 활성화에 대한 정향 에탄올 추출물의 억제 효과)

  • Chung, Joon-Hee;Kim, Yong-Min;Park, Jong-Phil;Kim, Tae-Yeon;Kim, Ee-Hwa
    • Korean Journal of Acupuncture
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    • v.31 no.1
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    • pp.14-19
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    • 2014
  • Objectives : In this report, we investigated the effect of ethanol extract of Syzygium aromaticum(L.) Merr. & Perry.(SAE) on the RBL-2H3 cell-mediated allergic response and studied its possible mechanisms of action. Methods : Cytotoxicity on RBL-2H3 cell was evaluated by MTT assay. Anti-allergic activity of SAE was assessed by ${\beta}$-Hexosaminidase and Histamine secretion, ${\beta}$- Hexosaminidase and Histamine secretion were measured by ELISA assay. Evaluate the mechanisms of effect of SAE on the secretion of degranulate mediators, we examined the effect of SAE on the activation of mitogen-activated protein kinases using western blot analysis. Results : SAE had no cytotoxicity on rat basophilic leukemia cell(RBL-2H3). Moreover SAE dose-dependently inhibited RBL-2H3 cell degranulation and histamine release. SAE specifically blocked the IgE-induced p38 mitogen-activated protein kinase activation. Conclusions : Our findings provide evidence that Syzygium aromaticum ethanol extract inhibits mast cell derived allergic reaction, and also demonstrate the involvement of p38 MAPK phosphorylation.

The Coffee Sliver Skin Extracts from Coffee Beans Exhibited Cosmetic Properties with Antioxiant Activity and Inhibitory Effects for Elastase, Collagenase and Tyrosinase (커피 은피 추출물의 항산화 효과와 엘라스타제, 콜라게나제 및 티로시나제 저해효과)

  • Lee, Kyung Eun;Son, Sang Hyeok;Kang, Sang Gu
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.44 no.1
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    • pp.39-48
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    • 2018
  • The coffee silver skin is a part of coffee beans. We report that the coffee sliver skin extracts exhibited cosmetic properties of antioxidant, anti-winkle and whitening effects. The ethanol extracts of silver skin showed free radical scavenging activity up to 92.26% in $50{\mu}g/mL$, especially against DPPH radical and ABTS radical cation. The silver skin extracts showed inhibitory effects for tyrosinase activity and DOPA oxidation in a dose-dependent manner, suggesting the extracts retain for the whitening property in cosmetics. The coffee silver skin extracts effectively inhibited the elastase and collagenase. Cytotoxicity of the coffee silver skin extracts was measured by the colorimetric MTS assay. The viability of the human keratinocytes (HaCaT) treated with the coffee silver skin extracts was same as that of untreated cells, indicating the extracts are safe to human cells. Here, we suggest that the silver skin extracts of coffee bean could be a potential natural substance for anti-winkle, whitening, antioxidant properties for cosmetics.

Sclerotherapy of Multiple Intraoral Venous Malformations with Use of Ethanolamine Oleate: A Case Report (구강내 발생한 다발성 정맥기형 병소에의 Ethanolamine Oleate 경화제 주사 후 치험례)

  • Kim, Tae-Kwang;Yang, Jae-Young;Choi, Seok-Tai;Jeon, Hee-Kyung;Leem, Dae-Ho;Baek, Jin-A;Shin, Hyo-Keun;Ko, Seung-O
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.34 no.6
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    • pp.488-493
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    • 2012
  • Hemangioma and vascular malformation are the most common benign tumors that are caused by congenitally or traumatic events. Theses tumors represent approximately 1/3~1/4 of all hemangiomas and vascular malformations in the head and neck. There are many forms of treatment for hemangioma and vascular malformation including closed observation, surgery, radiotherapy, laser therapy, steroid therapy, compression, embolization, and sclerotherapy. Ethanolamine oleate is an unsaturated fatty acid salt that has been used as a sclerosing agent because of its excellent thrombosing properties. This paper presents 1 case of intraoral multiple venous malformations treatment with 1.25% ethanolamine oleate (3.6~9.6 mg dose) intralesionally injected for 6 to 14 weeks over 2 week intervals. After the sclerotherapy, lesions almost completely disappeared without side effects. In conclusion, sclerotherapy using ethanolamine oleate is very effective against venous malformations, and sufficiently provides alternative support for surgical and other methods.

A brief method for preparation of gintonin-enriched fraction from ginseng

  • Choi, Sun-Hye;Jung, Seok-Won;Kim, Hyun-Sook;Kim, Hyeon-Joong;Lee, Byung-Hwan;Kim, Joon Yong;Kim, Jung-Hyun;Hwang, Sung Hee;Rhim, Hyewon;Kim, Hyoung-Chun;Nah, Seung-Yeol
    • Journal of Ginseng Research
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    • v.39 no.4
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    • pp.398-405
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    • 2015
  • Background: Ginseng has been used as a tonic for invigoration of the human body. In a previous report, we identified a novel candidate responsible for the tonic role of ginseng, designated gintonin. Gintonin induces $[Ca^{2+}]_i$ transient in animal cells via lysophosphatidic acid receptor activation. Gintonin-mediated $[Ca^{2+}]_i$ transient is linked to anti-Alzheimer's activity in transgenic Alzheimer's disease animal model. The previous method for gintonin preparation included multiple steps. The aim of this study is to develop a simple method of gintonin fraction with a high yield. Methods: We developed a brief method to obtain gintonin using ethanol and water. We extracted ginseng with fermentation ethanol and fractionated the extract with water to obtain water-soluble and water-insoluble fractions. The water-insoluble precipitate, rather than the water-soluble supernatant, induced a large $[Ca^{2+}]_i$ transient in primary astrocytes. We designated this fraction as gintonin-enriched fraction (GEF). Results: The yield of GEF was approximately 6-fold higher than that obtained in the previous gintonin preparation method. The apparent molecular weight of GEF, determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, was equivalent to that obtained in the previous gintonin preparation method. GEF induced $[Ca^{2+}]_i$ transient in cortical astrocytes. The effective dose (ED50) was $0.3{\pm}0.09{\mu}g/mL$. GEF used the same signal transduction pathway as gintonin during $[Ca^{2+}]_i$ transient induction in mouse cortical astrocytes. Conclusion: Because GEF can be prepared through water precipitation of ginseng ethanol extract and is easily reproducible with high yield, it could be commercially utilized for the development of gintoninderived functional health food and natural medicine.

Applications of Panax ginseng leaves-mediated gold nanoparticles in cosmetics relation to antioxidant, moisture retention, and whitening effect on B16BL6 cells

  • Jimenez-Perez, Zuly Elizabeth;Singh, Priyanka;Kim, Yeon-Ju;Mathiyalagan, Ramya;Kim, Dong-Hyun;Lee, Myoung Hee;Yang, Deok Chun
    • Journal of Ginseng Research
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    • v.42 no.3
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    • pp.327-333
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    • 2018
  • Background: Bioactive compounds in plant extracts are able to reduce metal ions to nanoparticles through the process of green synthesis. Panax ginseng is an oriental medicinal herb and an adaptogen which has been historically used to cure various diseases. In addition, the P. ginseng leaves-mediated gold nanoparticles are the value-added novel materials. Its potential as a cosmetic ingredient is still unexplored. The aim of this study was to evaluate the antioxidant, moisture retention and whitening properties of gold nanoparticles (PgAuNPs) in cosmetic applications. Methods: Cell-free experiments were performed to evaluate PgAuNP's antioxidant and moisture retention properties and inhibition activity on mushroom tyrosinase. Furthermore, in vitro cell cytotoxicity was evaluated using normal human dermal fibroblast and murine B16BL6 melanoma cells (B16) after treatment with increasing concentrations of PgAuNPs for 24 h, 48 h, and 72 h. Finally, in vitro cell assays on B16 cells were performed to evaluate the whitening effect of PgAuNPs through reduction of cellular melanin content and tyrosinase activity. Results: In vitro DPPH radical scavenging assay results revealed that PgAuNPs exhibited antioxidant activity in a dose-dependent manner. PgAuNPs exhibited moisture retention capacity and effectively inhibited mushroom tyrosinase. In addition, 3-(4,5-dimethyl-thiazol-2yl)-2,5-diphenyl tetrazolium bromide results revealed that PgAuNPs were not toxic to human dermal fibroblast and B16 cells; in addition, they significantly reduced melanin content, tyrosinase activity, and mRNA expression of melanogenesis-associated transcription factor and tyrosinase in B16 cells. Conclusion: Our study is the first report to provide evidence supporting that P. ginseng leaves-capped gold nanoparticles could be used as multifunctional ingredients in cosmetics.

A Case of Bronchiolitis Obliterans Organizing Pneumonia Associated with Wheezing (천식음이 동반된 Bronchiolitis Obliterans Organizing Pneumonia 1예)

  • Lee, Jae-Seok;Kim, Do-Jin;Ahn, Young-Soo;Lee, Sang-Moo;Kim, Hyeon-Tae;Uh, Soo-Taek;Kim, Yong-Hoon;Park, Choon-Sik
    • Tuberculosis and Respiratory Diseases
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    • v.40 no.6
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    • pp.730-735
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    • 1993
  • BOOP is a clinopathologic entity consisting of a flu-like illness, late inspiratory crackles, and pathologically granulation tissue plugs within lumens of small airways sometimes with complete obstruction of small airways and granulation tissue extending into alveolar ducts and alveoli with a variable degree of interstitial infiltration of mononuclear cells and accumulation of foamy macrophages in alveolar spaces in a patch distribution, and preservation of background architecture of the lung. It has patch infiltrates roentgenographically, and restrictive ventilatory defect pysiologically such as decreased vital capacity. and diffusing capacity. The BOOP has been observed in the context of collagen vascular disease, and other autoimmune disease secondary to treatment with penicillamine, bleomycin, acebutolol and amiodarone, following the inhalation of toxic fumes, after several infections including measles, pertussis and influenza and idiopathic. Clinically, response to coricosteroid therapy is good and relapse dose not occur if sufficient theraphy is good. A flu-like illness occurs in one third, cough in one third, cough with dyspnea in the remaining patients. Hemoptysis are rare. The physical examination reveales dry crackles in the majority of the patients with BOOP but rarely associated with wheezing. The duration of illness is less than 2 months in 75% of patients. With a brief review of literature, we report a case of the BOOP which is good response to steroid, but frequent relapse and assoicated with wheezing.

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